Histological Effects of Different Levels of Sorghum Grain on the Liver and Kidney of Ghezel×Arkhar-Merino Crossbred Lambs
Animal and Veterinary Sciences
Volume 2, Issue 4, July 2014, Pages: 130-134
Received: Jul. 21, 2014;
Accepted: Jul. 31, 2014;
Published: Aug. 10, 2014
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Hamid Karimi, Department of Basic science, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran
Hossein Daghigh Kia, Faculty of Agriculture, University of Tabriz, Tabriz, Iran
Ali Hosseinkhani, Faculty of Agriculture, University of Tabriz, Tabriz, Iran
Sorghum grains with variable concentrations of tannin (antinutritional factor) are becoming an increasingly important source of energy in animal nutrition. A large intake of tannins may cause gastrointestinal irritation, kidney and liver damage. Our experiment was conducted to study the effects of replacement different levels of barley grain with sorghum grain on the kidney and liver tissue. In this study sixteen male Ghezel×Arkhar-merino crossbred lambs were used. Dietary treatment were contain alfalfa hay (20% total DM), as roughage part of the diets and grain part (80% total DM) had different levels of the barley grain substituted with sorghum grain during 90 days. Lambs were randomly assigned to to receive one of the four dietary treatments in a completely randomized design assignment, in which sorghum grain was used in the levels of 60, 70 and 80 percent of total ration. The gross examination of kidney and liver did not reveal any significant histological changes. The microscopic results showed that, increases of the sorghum grain levels resulting histological changes in kidney and liver parenchyma. Histological examination of kidney of group A showed a little change as degeneration of brush borders in some of the proximal convoluted tubule (PCT) cells. In B group, results appeared which brush borders of PCT were destroyed therefore the high cuboidal cells of PCT were observed as low cuboidal epithelium and diameter of PCT increased. Hemorrhage and congestion is not observed in cortex but it was observed in medulla. In C group, degeneration of cells of PCT and DCT, hemorrhage in cortex, congestion in medulla which they are more in area near apex of renal pyramids were observed. Decrease length of cuboidal cells in medullary collecting ducts. Amyloidal sedimentation in PCT and DCT. In the liver of A and B group animals, histological structure of liver was normal, but sinusoidal abnormality was observed in A group and only a little congestion observed in some central venules in B group animals. Liver of C group animals had many histological changes as shrinkage of hepatocyte, degeneration and necrosis of hepatocytes around central venules, degeneration of some hepatic sinusoids, enlargement hepatic sinusoid in order to congestion and reduce length of hepatocytes, hemorrhage around central venuls, a little distribution of cirrhosis. In generally high level of sorghum grains in diet can induced hisological changs in kidney and liver of sheep.
Hossein Daghigh Kia,
Histological Effects of Different Levels of Sorghum Grain on the Liver and Kidney of Ghezel×Arkhar-Merino Crossbred Lambs, Animal and Veterinary Sciences.
Vol. 2, No. 4,
2014, pp. 130-134.
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