Electrophoresis Study of Wheat (<i>Triticumaestivum</i> L.) Protein Changes Under Salinity Stress

Neda Sobhanian; Hassan Pakniyat; Mahmood Ahmadi Kordshooli; Saeideh Dorostkar; Massumeh Aliakbari; Ziba Faghih Nasiri

doi:doi:10.11648/j.sr.20160402.12

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Electrophoresis Study of Wheat (Triticumaestivum L.) Protein Changes Under Salinity Stress

Neda Sobhanian, Hassan Pakniyat, Mahmood Ahmadi Kordshooli, Saeideh Dorostkar, Massumeh Aliakbari, Ziba Faghih Nasiri

Published in Science Research (Volume 4, Issue 2)

Received: 26 November 2015 Accepted: 29 December 2015 Published: 9 March 2016

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Abstract

Salinity problem with its vast spread on the earth is one of the main factors which limits crop production. One of the methods to overcome this problem is taking advantage of the resistant genotypes. Investigation of changes resulting from the stress in an electrophoretic profile of proteins and understanding its relation with the tolerance and susceptibility of cultivars is an important criterion for identifying such cultivars. In this study, 4 wheat cultivars with different degrees of tolerance were grown in hydroponic culture under salinity treatment (0, 70, 140 and 210 mM NaCl). Leaf sampling was done on 5 leaf stage. Studying the electrophoretic pattern of the leaf soluble proteins in salinity and control treatments showed fundamental similarities among the cultivars. No polypeptide bands belonging to the specific cultivars or to one of the salinity treatments were observed. The study of protein changes by electrophoretic analysis under salinity treatment may be useful for understanding the salinity tolerance of genotypes.

Published in	Science Research (Volume 4, Issue 2)
DOI	10.11648/j.sr.20160402.12
Page(s)	33-36
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Salinity, Electrophoretic Pattern, Leaf Soluble Proteins, Salt Tolerance

References

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[2]	Bradford MM. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Annual review of Biochemistry 72, 247-254.
[3]	Bressan RA, SinghNK, Handa AK, Mount R, Clithero J, Hasegawa JH. 1987. Stability of altered genetic expression in cultured plant cells adapted to salt. In: Monit L, Proceddu E. EEc symposium on "Drought Resistance in Plants: Genetic and Physiological Aspects". 41-57.
[4]	Flowers TJ, Hajibagheri MA, Clipson NJW. 1986. Halophytes. The Quarterly Review of Biology 61, 313-337.
[5]	Flowers TJ. 2004. Improving crop salt tolerance. Journal of Experimental Botany 55, 307-319.
[6]	Gao L, Yan X, Li X, Guo G, Hu Y, Ma W, Yan Y. 2011. Proteome analysis of wheat leaf under salt stress by two-dimensional difference gel electrophoresis (2D-DIGE). Phytochemistry 72 (10), 1180-1191.
[7]	Gorham J, McDonell E, Budrewicz E, WynJones RG. 1985. Salt tolerance in the Triticeae: growth and solute accumulation in leaves of Thinopyrumbessarabicum. Journal of Experimental Botany 36, 1021-1031.
[8]	Hurkman WJ, Tanaka CK. 1987. The effects of salt on the pattern of protein synthesis in barely roots. Plant Physiology 83, 517-521.
[9]	Mahajan S, Tuteja N. 2005. Cold, salinity and drought stresses: an overview. Archives Biochemistry Biophysics 444, 139–158.
[10]	Maslenkova LT. 1992. Changes polypeptides patterns of barley seedlings exposed to jasmonic acid and salinity. Plant Physiology 98, 700-707.
[11]	Moreno J, Garcia-Murria MJ, Marin-Navarro J. 2008. Redox modulation of rubisco conformation and activity through its cysteine residues. Journal of Experimental Botany 59, 1605-1614.
[12]	Munns R. 2002. Comparing physiology of salt and water stress. Plant Cell and Environment 25, 239-250.
[13]	Nato A, Mirshahi A, Cavalacnte JM. 1995. Are Arestin-like proteins involve in plant signal transduction pathway? Plant Molecular Cell Biology, 519-524.
[14]	Rashid A, Qureshi RH, Hollington PA, Wyn Jones RG. 1998. Comparative responses of wheat cultivars to salinity. Journal of Agronomy and Crop Science 182, 199-207.
[15]	Yıldız M. 2007. Two-dimensional electrophoretic analysis of soluble leaf proteins of a salt ‎sensitive (Triticumaestivum) and a salt-tolerant (Triticum durum) cultivar in response to NaCl‎ stress. Journal of Integrative Plant Biology 49, 975-981.‎

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APA Style

Neda Sobhanian, Hassan Pakniyat, Mahmood Ahmadi Kordshooli, Saeideh Dorostkar, Massumeh Aliakbari, et al. (2016). Electrophoresis Study of Wheat (Triticumaestivum L.) Protein Changes Under Salinity Stress. Science Research, 4(2), 33-36. https://doi.org/10.11648/j.sr.20160402.12

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ACS Style

Neda Sobhanian; Hassan Pakniyat; Mahmood Ahmadi Kordshooli; Saeideh Dorostkar; Massumeh Aliakbari, et al. Electrophoresis Study of Wheat (Triticumaestivum L.) Protein Changes Under Salinity Stress. Sci. Res. 2016, 4(2), 33-36. doi: 10.11648/j.sr.20160402.12

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AMA Style

Neda Sobhanian, Hassan Pakniyat, Mahmood Ahmadi Kordshooli, Saeideh Dorostkar, Massumeh Aliakbari, et al. Electrophoresis Study of Wheat (Triticumaestivum L.) Protein Changes Under Salinity Stress. Sci Res. 2016;4(2):33-36. doi: 10.11648/j.sr.20160402.12

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@article{10.11648/j.sr.20160402.12,
  author = {Neda Sobhanian and Hassan Pakniyat and Mahmood Ahmadi Kordshooli and Saeideh Dorostkar and Massumeh Aliakbari and Ziba Faghih Nasiri},
  title = {Electrophoresis Study of Wheat (Triticumaestivum L.) Protein Changes Under Salinity Stress},
  journal = {Science Research},
  volume = {4},
  number = {2},
  pages = {33-36},
  doi = {10.11648/j.sr.20160402.12},
  url = {https://doi.org/10.11648/j.sr.20160402.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.sr.20160402.12},
  abstract = {Salinity problem with its vast spread on the earth is one of the main factors which limits crop production. One of the methods to overcome this problem is taking advantage of the resistant genotypes. Investigation of changes resulting from the stress in an electrophoretic profile of proteins and understanding its relation with the tolerance and susceptibility of cultivars is an important criterion for identifying such cultivars. In this study, 4 wheat cultivars with different degrees of tolerance were grown in hydroponic culture under salinity treatment (0, 70, 140 and 210 mM NaCl). Leaf sampling was done on 5 leaf stage. Studying the electrophoretic pattern of the leaf soluble proteins in salinity and control treatments showed fundamental similarities among the cultivars. No polypeptide bands belonging to the specific cultivars or to one of the salinity treatments were observed. The study of protein changes by electrophoretic analysis under salinity treatment may be useful for understanding the salinity tolerance of genotypes.},
 year = {2016}
}

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TY  - JOUR
T1  - Electrophoresis Study of Wheat (Triticumaestivum L.) Protein Changes Under Salinity Stress
AU  - Neda Sobhanian
AU  - Hassan Pakniyat
AU  - Mahmood Ahmadi Kordshooli
AU  - Saeideh Dorostkar
AU  - Massumeh Aliakbari
AU  - Ziba Faghih Nasiri
Y1  - 2016/03/09
PY  - 2016
N1  - https://doi.org/10.11648/j.sr.20160402.12
DO  - 10.11648/j.sr.20160402.12
T2  - Science Research
JF  - Science Research
JO  - Science Research
SP  - 33
EP  - 36
PB  - Science Publishing Group
SN  - 2329-0927
UR  - https://doi.org/10.11648/j.sr.20160402.12
AB  - Salinity problem with its vast spread on the earth is one of the main factors which limits crop production. One of the methods to overcome this problem is taking advantage of the resistant genotypes. Investigation of changes resulting from the stress in an electrophoretic profile of proteins and understanding its relation with the tolerance and susceptibility of cultivars is an important criterion for identifying such cultivars. In this study, 4 wheat cultivars with different degrees of tolerance were grown in hydroponic culture under salinity treatment (0, 70, 140 and 210 mM NaCl). Leaf sampling was done on 5 leaf stage. Studying the electrophoretic pattern of the leaf soluble proteins in salinity and control treatments showed fundamental similarities among the cultivars. No polypeptide bands belonging to the specific cultivars or to one of the salinity treatments were observed. The study of protein changes by electrophoretic analysis under salinity treatment may be useful for understanding the salinity tolerance of genotypes.
VL  - 4
IS  - 2
ER  -

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Author Information

Neda Sobhanian

Department of Crop Production and Plant Breeding, Shiraz University, Shiraz, Iran
Hassan Pakniyat

Department of Crop Production and Plant Breeding, Shiraz University, Shiraz, Iran
Mahmood Ahmadi Kordshooli

Department of Crop Production and Plant Breeding, Shiraz University, Shiraz, Iran
Saeideh Dorostkar

Department of Crop Production and Plant Breeding, Shiraz University, Shiraz, Iran
Massumeh Aliakbari

Department of Crop Production and Plant Breeding, Shiraz University, Shiraz, Iran
Ziba Faghih Nasiri

Department of Crop Production and Plant Breeding, Shiraz University, Shiraz, Iran

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APA Style

Neda Sobhanian, Hassan Pakniyat, Mahmood Ahmadi Kordshooli, Saeideh Dorostkar, Massumeh Aliakbari, et al. (2016). Electrophoresis Study of Wheat (Triticumaestivum L.) Protein Changes Under Salinity Stress. Science Research, 4(2), 33-36. https://doi.org/10.11648/j.sr.20160402.12

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ACS Style

Neda Sobhanian; Hassan Pakniyat; Mahmood Ahmadi Kordshooli; Saeideh Dorostkar; Massumeh Aliakbari, et al. Electrophoresis Study of Wheat (Triticumaestivum L.) Protein Changes Under Salinity Stress. Sci. Res. 2016, 4(2), 33-36. doi: 10.11648/j.sr.20160402.12

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AMA Style

Neda Sobhanian, Hassan Pakniyat, Mahmood Ahmadi Kordshooli, Saeideh Dorostkar, Massumeh Aliakbari, et al. Electrophoresis Study of Wheat (Triticumaestivum L.) Protein Changes Under Salinity Stress. Sci Res. 2016;4(2):33-36. doi: 10.11648/j.sr.20160402.12

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@article{10.11648/j.sr.20160402.12,
  author = {Neda Sobhanian and Hassan Pakniyat and Mahmood Ahmadi Kordshooli and Saeideh Dorostkar and Massumeh Aliakbari and Ziba Faghih Nasiri},
  title = {Electrophoresis Study of Wheat (Triticumaestivum L.) Protein Changes Under Salinity Stress},
  journal = {Science Research},
  volume = {4},
  number = {2},
  pages = {33-36},
  doi = {10.11648/j.sr.20160402.12},
  url = {https://doi.org/10.11648/j.sr.20160402.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.sr.20160402.12},
  abstract = {Salinity problem with its vast spread on the earth is one of the main factors which limits crop production. One of the methods to overcome this problem is taking advantage of the resistant genotypes. Investigation of changes resulting from the stress in an electrophoretic profile of proteins and understanding its relation with the tolerance and susceptibility of cultivars is an important criterion for identifying such cultivars. In this study, 4 wheat cultivars with different degrees of tolerance were grown in hydroponic culture under salinity treatment (0, 70, 140 and 210 mM NaCl). Leaf sampling was done on 5 leaf stage. Studying the electrophoretic pattern of the leaf soluble proteins in salinity and control treatments showed fundamental similarities among the cultivars. No polypeptide bands belonging to the specific cultivars or to one of the salinity treatments were observed. The study of protein changes by electrophoretic analysis under salinity treatment may be useful for understanding the salinity tolerance of genotypes.},
 year = {2016}
}

Copy | Download

TY  - JOUR
T1  - Electrophoresis Study of Wheat (Triticumaestivum L.) Protein Changes Under Salinity Stress
AU  - Neda Sobhanian
AU  - Hassan Pakniyat
AU  - Mahmood Ahmadi Kordshooli
AU  - Saeideh Dorostkar
AU  - Massumeh Aliakbari
AU  - Ziba Faghih Nasiri
Y1  - 2016/03/09
PY  - 2016
N1  - https://doi.org/10.11648/j.sr.20160402.12
DO  - 10.11648/j.sr.20160402.12
T2  - Science Research
JF  - Science Research
JO  - Science Research
SP  - 33
EP  - 36
PB  - Science Publishing Group
SN  - 2329-0927
UR  - https://doi.org/10.11648/j.sr.20160402.12
AB  - Salinity problem with its vast spread on the earth is one of the main factors which limits crop production. One of the methods to overcome this problem is taking advantage of the resistant genotypes. Investigation of changes resulting from the stress in an electrophoretic profile of proteins and understanding its relation with the tolerance and susceptibility of cultivars is an important criterion for identifying such cultivars. In this study, 4 wheat cultivars with different degrees of tolerance were grown in hydroponic culture under salinity treatment (0, 70, 140 and 210 mM NaCl). Leaf sampling was done on 5 leaf stage. Studying the electrophoretic pattern of the leaf soluble proteins in salinity and control treatments showed fundamental similarities among the cultivars. No polypeptide bands belonging to the specific cultivars or to one of the salinity treatments were observed. The study of protein changes by electrophoretic analysis under salinity treatment may be useful for understanding the salinity tolerance of genotypes.
VL  - 4
IS  - 2
ER  -

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