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Susceptibility of Vegetatively Propagated Khaya anthoteca to Arbuscular Mycorrhizae Fungi (AMF) Soil Inoculum Infection

Received: 03 August 2014    Accepted: 19 August 2014    Published: 15 February 2015
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Abstract

Stem tip cuttings of Khaya anthoteca seedlings were experimented for the trapping of Arbuscular Mycorrhizae Fungi (AMF). This study aimed to determine the effect of hormone and wounding treatments on the vegetative propagation of K. anthoteca seedlings and the susceptibility of these cuttings to AMF soil inoculums infection compared to some other bait species such as Sorghum bicolor and Puereria javanica. The results indicated that, K. anthoteca can successfully be propagated through stem cutting with only wounding and without hormone (auxin) applications. Analysis of variance tested at 5% significance level revealed no differences between the treatment means of hormone and wounding on the number of roots. Again, the lengths of the longest roots were not significantly different over the control treatment. In the trapping experiment, there were no significant differences in terms of root segment colonization between K. anthoteca cuttings and the other trapping species. This observation was however, frequent in the young and meristematic segments of the roots for K. anthoteca. An average root colonization of 32% was observed for K. anthoteca and the highest recorded by S. bicolor of 43%. Thus, about a third of root segments of K. anthoteca were colonized by AMF. There was also a significant positive correlation between percentage root segment colonization and the number of counted spores. The study concludes that wounding of K. anthoteca six-month old seedlings prior to vegetative propagation by cutting play vital role with respect to the rooting ability of the propagules compared to non-wounded propagules and also not significantly different from that of synthetic hormone application. Again, K. anthoteca is susceptible to AMF just like most tropical plant species in Indonesia. Finally, AMF soil inoculums can also be a good source of inoculants for establishing K. anthoteca in areas of degraded lands for revegetation purposes.

DOI 10.11648/j.sr.20150301.13
Published in Science Research (Volume 3, Issue 1, February 2015)
Page(s) 13-18
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This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Khaya anthoteca, Vegetative propagation, Hormone, Wounding, AMF soil inoculums

References
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[2] Berhe D, Negash L. 1998. Asexual propagation of Juniperus procera from Ethiopia: a contribution to the conservation of African pencil cedar,” Forest Ecology and Management. 112: 179–190
[3] Bever JD et al. 1996. Host-dependent sporulation and species diversity of arbuscular mycorrhizal fungi in mown grassland. J. Ecol. 84, 71–82
[4] Bhardwaj DR, Mishra VK. 2005 Vegetative propagation of Ulmus villosa: effects of plant growth regulators, collection time, type of donor and position of shoot on adventitious root formation in stem cuttings,” New Forests. 29: 105–116.
[5] Bonga JM, von Aderkas P. 1993. Rejuvenation of tissues from mature conifers and its implications for propagation in vitro, [In: Clonal Forestry I, Genetics and Biotechnology, M. R. Ahuja and W. J. Libby, Eds., Springer, Berlin Heidelberg, Germany. Pp. 182–199]
[6] Browne RD, Davidson CG, Steeves TA, Dunstan DI. 1997. Effects of ortet age on adventitious rooting of jack pine (Pinus banksiana) long-shoot cuttings, Canadian Journal of Forest Research. 27: 91–96.
[7] Brundrett M, Bougher N, Dell B, Grove T, Malajczuk N. 1996. Working with Mycorrhizae in Forestry and Agriculture. AClAR Monograph. Pirie Printers, Canberra, Australia Pp 179-185
[8] Douds DD, Millner PD. 1999. Biodiversity of arbuscular mycorrhizal fungi in agroecosystems. Agric. Ecosyst. Environ. 74, 77–93
[9] Eom AH et al. 2000. Host plant species effects on arbuscular mycorrhizal fungal communities in tall grass prairie. Oecologia 122, 435–444
[10] Gaur A, Adholeya A. 2004. Prospect of arbuscular mycorrhizal fungi in phytoremediation of heavy metal contaminated soils. Curr. Sci., 86: 528.
[11] Greenwood MS, Hutchison KW. 1993. Maturation as a developmental process, in Clonal forestry I, Genetics and Biotechnology, M. R. Ahuja and W. J. Libby, Eds., Springer, Berlin Heidelberg, Germany, pp. 14–33.
[12] Hartmann HT, Kester DE, Davies FT, Geneve RL. 2002. Hartmann and Kester’s Plant Propagation: Principles and Practices, 7th edition, Prentice Hall, New Jersey, USA, Pp 300
[13] Hartmann HT, Kester DE, Davies FT, Geneve RL. 1997. Plant propagation; principles and practices. 6th Ed. USA, Prentice-Hall Inc. Pp 298-355
[14] Hawthorne W. 1998. Khaya anthotheca. The IUCN Red List of Threatened Species. Version 2014.3. www.iucnredlist.org. Downloaded on 24 January 2015.
[15] Hopkins GW, Norman Hüner NPA. 2009. Introduction to Plant Physiology. 4th Ed. John Wiley & Sons, Inc. USA. Pp 338-448.
[16] Jaenicke H, Jan Beniest (ed) 2002. Vegetative Tree Propagation in Agroforestry; Training Guidelines and References. Kul Graphics Ltd, Nairobi, Kenya. Pp 1-4
[17] Kiers ET et al. 2000. Differential effects of tropical arbuscular mycorrhizal fungal inocula on root colonization and tree seedling growth: implications for tropical forest diversity. Ecol. Lett. 3, 106–113
[18] Leakey RRB, Mesen JF, Tchoundjeu Z. 1990. Low-technology techniques for the vegetative propagation of tropical trees. Commonwealth Forest Review 69: 247-257
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[20] Smith SE, Read DJ. 2008. Mycorrhizal Symbiosis, 3rd ed. Elsevier and Academic, New York
[21] Smith SE, Read DJ. 1996. Mycorrhizal Symbiosis. Academic Press, San Diego.
[22] Toju H, Sato H, Yamamoto S, Kadowaki K, Tanabe AS, Yazawa S, Nishimura O, Agata K. 2013. A massively parallel pyrosequencing analysis of the association specificity of root-associated fungi and their host plants. Journal of Ecology and Evolution 3: 112–124
[23] Wilson PJ, van Staden J. 1990. Rhizocaline, rooting co-factor and the concept of promoters and inhibitors of adventitious rooting; a review. Annals Botany 66:476-490.
Author Information
  • Department of Silviculture, Faculty of Forestry, Bogor Agricultural University, Dramaga Campus –Bogor 16680, Indonesia

  • Department of Silviculture, Faculty of Forestry, Bogor Agricultural University, Dramaga Campus –Bogor 16680, Indonesia; SEMEO Biotrop, Jalan Raya Tajur, Bogor 16134, Indonesia

  • Department of Silviculture, Faculty of Forestry, Bogor Agricultural University, Dramaga Campus –Bogor 16680, Indonesia

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    Philip Worlanyo Dugbley, Irdika Mansur, Basuki Wasis. (2015). Susceptibility of Vegetatively Propagated Khaya anthoteca to Arbuscular Mycorrhizae Fungi (AMF) Soil Inoculum Infection. Science Research, 3(1), 13-18. https://doi.org/10.11648/j.sr.20150301.13

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    Philip Worlanyo Dugbley; Irdika Mansur; Basuki Wasis. Susceptibility of Vegetatively Propagated Khaya anthoteca to Arbuscular Mycorrhizae Fungi (AMF) Soil Inoculum Infection. Sci. Res. 2015, 3(1), 13-18. doi: 10.11648/j.sr.20150301.13

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    AMA Style

    Philip Worlanyo Dugbley, Irdika Mansur, Basuki Wasis. Susceptibility of Vegetatively Propagated Khaya anthoteca to Arbuscular Mycorrhizae Fungi (AMF) Soil Inoculum Infection. Sci Res. 2015;3(1):13-18. doi: 10.11648/j.sr.20150301.13

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  • @article{10.11648/j.sr.20150301.13,
      author = {Philip Worlanyo Dugbley and Irdika Mansur and Basuki Wasis},
      title = {Susceptibility of Vegetatively Propagated Khaya anthoteca to Arbuscular Mycorrhizae Fungi (AMF) Soil Inoculum Infection},
      journal = {Science Research},
      volume = {3},
      number = {1},
      pages = {13-18},
      doi = {10.11648/j.sr.20150301.13},
      url = {https://doi.org/10.11648/j.sr.20150301.13},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.sr.20150301.13},
      abstract = {Stem tip cuttings of Khaya anthoteca seedlings were experimented for the trapping of Arbuscular Mycorrhizae Fungi (AMF). This study aimed to determine the effect of hormone and wounding treatments on the vegetative propagation of K. anthoteca seedlings and the susceptibility of these cuttings to AMF soil inoculums infection compared to some other bait species such as Sorghum bicolor and Puereria javanica. The results indicated that, K. anthoteca can successfully be propagated through stem cutting with only wounding and without hormone (auxin) applications. Analysis of variance tested at 5% significance level revealed no differences between the treatment means of hormone and wounding on the number of roots. Again, the lengths of the longest roots were not significantly different over the control treatment. In the trapping experiment, there were no significant differences in terms of root segment colonization between K. anthoteca cuttings and the other trapping species. This observation was however, frequent in the young and meristematic segments of the roots for K. anthoteca. An average root colonization of 32% was observed for K. anthoteca and the highest recorded by S. bicolor of 43%. Thus, about a third of root segments of K. anthoteca were colonized by AMF. There was also a significant positive correlation between percentage root segment colonization and the number of counted spores. The study concludes that wounding of K. anthoteca six-month old seedlings prior to vegetative propagation by cutting play vital role with respect to the rooting ability of the propagules compared to non-wounded propagules and also not significantly different from that of synthetic hormone application. Again, K. anthoteca is susceptible to AMF just like most tropical plant species in Indonesia. Finally, AMF soil inoculums can also be a good source of inoculants for establishing K. anthoteca in areas of degraded lands for revegetation purposes.},
     year = {2015}
    }
    

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    T1  - Susceptibility of Vegetatively Propagated Khaya anthoteca to Arbuscular Mycorrhizae Fungi (AMF) Soil Inoculum Infection
    AU  - Philip Worlanyo Dugbley
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    PB  - Science Publishing Group
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    AB  - Stem tip cuttings of Khaya anthoteca seedlings were experimented for the trapping of Arbuscular Mycorrhizae Fungi (AMF). This study aimed to determine the effect of hormone and wounding treatments on the vegetative propagation of K. anthoteca seedlings and the susceptibility of these cuttings to AMF soil inoculums infection compared to some other bait species such as Sorghum bicolor and Puereria javanica. The results indicated that, K. anthoteca can successfully be propagated through stem cutting with only wounding and without hormone (auxin) applications. Analysis of variance tested at 5% significance level revealed no differences between the treatment means of hormone and wounding on the number of roots. Again, the lengths of the longest roots were not significantly different over the control treatment. In the trapping experiment, there were no significant differences in terms of root segment colonization between K. anthoteca cuttings and the other trapping species. This observation was however, frequent in the young and meristematic segments of the roots for K. anthoteca. An average root colonization of 32% was observed for K. anthoteca and the highest recorded by S. bicolor of 43%. Thus, about a third of root segments of K. anthoteca were colonized by AMF. There was also a significant positive correlation between percentage root segment colonization and the number of counted spores. The study concludes that wounding of K. anthoteca six-month old seedlings prior to vegetative propagation by cutting play vital role with respect to the rooting ability of the propagules compared to non-wounded propagules and also not significantly different from that of synthetic hormone application. Again, K. anthoteca is susceptible to AMF just like most tropical plant species in Indonesia. Finally, AMF soil inoculums can also be a good source of inoculants for establishing K. anthoteca in areas of degraded lands for revegetation purposes.
    VL  - 3
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