Green Synthesis and Antibacterial Properties of Silver Nanoparticles Using Sudanese Azadirachta indica Leaves Extract
Volume 8, Issue 1, February 2020, Pages: 14-19
Received: Jan. 26, 2020;
Accepted: Feb. 19, 2020;
Published: Apr. 28, 2020
Views 378 Downloads 203
Ayat Ahmed Alrasheid, Department of Pharmacognosy, Faculty of Pharmacy, University of Medical Sciences and Technology, Khartoum, Sudan
Sahar Hussein Eltilib, Department of Pharmacology, Faculty of Pharmacy, University of Medical Sciences and Technology, Khartoum, Sudan
Shimaa Abdel Rahman Ahmed, Department of Pharmaceutical Analysis, Faculty of Pharmacy, University of Medical Sciences and Technology, Khartoum, Sudan
Alaa Abdulmoneim Mohamed, Department of Clinical Pharmacy, Faculty of Pharmacy, University of Medical Sciences and Technology, Khartoum, Sudan
Hiba Abbas Widatalla, Department of Pharmacology, Faculty of Pharmacy, University of Medical Sciences and Technology, Khartoum, Sudan
Layla Fathi Yassin, Department of Pharmaceutical Analysis, Faculty of Pharmacy, University of Medical Sciences and Technology, Khartoum, Sudan
Maida Musa Ali, Department of Pharmaceutical Analysis, Faculty of Pharmacy, University of Medical Sciences and Technology, Khartoum, Sudan
Marvit Osman Widdatallh, Department of Pharmacology, Faculty of Pharmacy, University of Medical Sciences and Technology, Khartoum, Sudan
Follow on us
Nanoparticles have emerged as a key player in modern medicine and involved in drug delivery, it can be manufactured using various approaches including chemical, physical, and biological methods. Nanoparticles are generally characterized by their size, morphology and surface charge using advanced microscopic techniques such as SEM and TEM. This study was aimed for synthesis and characterization of silver nanoparticle and to detect their antibacterial activity using Azadirachta indica (Neem) leaves extract. The results showed a visible change of colour after the addition of Neem leaf extract to AgNO3 solution to dark brown which indicates the formation of silver nanoparticle. UV spectrophotometer showed absorbance peak in range of 390-700 nm, the result revealed the highest peak showed by concentration 1 ml at 428.80 nm. SEM and XRD analysis were used to characterize synthesize silver nanoparticles. The antibacterial activity against Gram positive Staphylococcus aureus and Gram negative Escherichia coli and Klebsiella spp Microorganisms was carried out using disc diffusion method, the results showed that the highest activity was 14 mm against S. aureus. A. indica silver nanoparticles can be used as a new nano drug in formulation and delivery. The synthesized silver nanoparticles are extremely important in various areas of life and are widely used for the benefit of human being.
Green Synthesis, Silver Nanoparticles, Azadirachta indica, SEM, XRD, Antibacterial Properties, Sudan
To cite this article
Ayat Ahmed Alrasheid,
Sahar Hussein Eltilib,
Shimaa Abdel Rahman Ahmed,
Alaa Abdulmoneim Mohamed,
Hiba Abbas Widatalla,
Layla Fathi Yassin,
Maida Musa Ali,
Marvit Osman Widdatallh,
Green Synthesis and Antibacterial Properties of Silver Nanoparticles Using Sudanese Azadirachta indica Leaves Extract, Science Research.
Vol. 8, No. 1,
2020, pp. 14-19.
Copyright © 2020 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/
) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Sriram, M. I., Kalishwaralal, K., Barathmanikanth, S and Gurunathani, S. (2012). Size-based cytotoxicity of silver nanoparticles in bovine retinal endothelial cells. Nanosci Methods; 1 (1): 56–77.
Riley, M. A., Robinson, S. M., Roy, C. M., Dennis, M., Liu, V., Dorit, R. L. (2012). Resistance is futile: the bacteriocin model for addressing the antibiotic resistance challenge. Biochem Soc Trans. 40 (6): 1438–1442.
Ovais, M., Khalil, A. T., Raza, A., Khan, M. A., Ahmad, I., Islam, N. U., Saravanan, M., Ubaid, M. F., Ali, M and Shinwari, Z. K. (2016). Green synthesis of silver nanoparticles via plant extracts: beginning a new era in cancer theranostics. Nanomedicine; 12 (23): 3157-77.
Khan, M., Adil, S. F., Musarrat, J., Al-Khedhairy, A., Al-Warthan, A., HSiddiqui, M. R., Rafiq, M and Alkhathlan, H. (2014). Antibacterial properties of silver nanoparticles synthesized using Pulicaria glutinosa plant extract as a green bioreductant, Mujeeb Khan Shams Tabrez Khan, International Journal of Nanomedicine; 2014: 9 3551–3565.
Bulent, U., Turkdemir, M. H., Cicek, A and Mete, A. (2015). Role of irradiation in the green synthesis of silver nanoparticles mediated by fig (Ficus carica) leaf extract. Spectrochim Acta Part A Mol Biomol Spectrosc; 135: 153–161.
Siddiqi, K. S., Husen, A., Rao, R. A. K. (2018). A review on biosynthesis of silver nanoparticles and their biocidal properties. J Nanobiotechnol; 16: 14.
Karar, M. G and Kuhnert, N. (2017). Herbal drugs from Sudan: Traditional uses and phytoconstituents. Pharmacognosy reviews; 11 (22): 83.
Abdeen, S., Isaac, R. R., Geo, S., Sornalekshmi, S., Rose, A and Praseetha, P. K. (2013). Evaluation of Antimicrobial Activity of Biosynthesized Iron and Silver Nanoparticles Using the Fungi Fusarium Oxysporum and Actinomycetes sp. on Human Pathogens. Nano Biomedicine & Engineering; 1; 5 (1).
Mahfuzul Hoque, M. D., Bari, M. L., Inatsu, Y., Juneja, V. K. and Kawamoto, S. (2007). Antibacterial activity of guava (Psidium guajava L.) and neem (Azadirachta indica A. Juss.) extracts against foodborne pathogens and spoilage bacteria. Foodborne pathogens and disease. Dec 1; 4 (4): 481-8.
Veerasamy, R., Xin, T. Z., Gunasagaran, S., Xiang, T. F., Yang, E. F., Jeyakumar, N. and Dhanaraj, S. A. (2011). Biosynthesis of silver nanoparticles using mangosteen leaf extract and evaluation of their antimicrobial activities. Journal of Saudi Chemical Society; 1; 15 (2): 113-20.
Vijayakumar, M., Priya, K., Nancy, F. T., Noorlidah, A. and Ahmed, A. B. A. (2013). Biosynthesis, characterisation and anti-bacterial effect of plant-mediated silver nanoparticles using Artemisia nilagirica. Industrial Crops and Products, 41, 235-240.
Bindhu, M. R., &Umadevi, M. (2013). Synthesis of monodispersed silver nanoparticles using Hibiscus cannabinus leaf extract and its antimicrobial activity. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 101, 184-190.