Please enter verification code
Confirm
Comparative Investigation of Border Disease Virus Infection in Sheep Flocks with Abortion Problems in Konya Province
Science Research
Volume 2, Issue 5, October 2014, Pages: 119-124
Received: Sep. 30, 2014; Accepted: Oct. 15, 2014; Published: Oct. 30, 2014
Views 3152      Downloads 152
Authors
Oguzhan Avci, Department of Virology, Faculty of Veterinary Medicine, University of Selcuk, 42075, Konya, Turkey
Sibel Yavru, Department of Virology, Faculty of Veterinary Medicine, University of Selcuk, 42075, Konya, Turkey
Article Tools
Follow on us
Abstract
The aim of the present study was to determine the serological and virological status of animals persistently infected (PI) with border disease virus (BDV), with focus on the prevalence of pestiviruses in sheep and lambs. This study is the first to our knowledge, to investigate the role of viral infections in abortions in the sheep population of Konya province. The prevalence of antibodies against BDV was as follows: sheep, 79%; lambs, 43.4%; and rams, 6%. Of 1000 leukocyte (sheep) samples examined with a direct ELISA, 11 (1.1%) (first sampling) were antibody-positive. Three sheep were determined to be PI and were sacrificed. Of 1011 sheep leukocyte samples, 14 were examined using a direct immunoperoxidase (IP) assay for BDV antigen, and 11 of 327 (3.36%) tissue samples were positive. The BDV genome was detected in 14 of 63 (22.2%) sheep leukocyte samples and 11 of 327 (3.36%) tissue samples by one-step RT-PCR. The BDV genome could not be detected in any vaginal swab samples. The sensitivity and specificity rates between direct ELISA and direct IP were respectively 100% and 98.8% and the values were, respectively, 78.57% and 98.6% between direct ELISA and one-step RT-PCR and 100% and 97.5% between one-step RT-PCR and direct IP. In conclusion it was determinate that BDV has an important role as an etiologic agent in abortion causes in sheep population in Konya.
Keywords
Border Disease Virus, Direct ELISA, Immunoperoxidase, One Step RT-PCR
To cite this article
Oguzhan Avci, Sibel Yavru, Comparative Investigation of Border Disease Virus Infection in Sheep Flocks with Abortion Problems in Konya Province, Science Research. Vol. 2, No. 5, 2014, pp. 119-124. doi: 10.11648/j.sr.20140205.17
References
[1]
Krametter-Froetscher, R., Kohler, H., Benetka, V., Moestl, K., Golja, F., Vilcek, S. and Baumgartner W. (2007). Influence of communal alpine pasturing on the spread of pestiviruses among Sheep and Goats in Austria: First identification of border disease virus in Austria. Zoonoses and Public Health, 54: 209-213.
[2]
Kale, M., Yavru, S., Ata, A., Kocamuftuoglu, M., Yapici, O. and Hasircioglu, S. (2011). Bovine viral diarrhoea virus infection in relation to fertility in heifers. Journal of Veterinary Medical Science, 73: 331-336.
[3]
Rasmussen, T. B., Reimann, I., Hoffmann, B., Depner, K., Uttenthal, A. and Beer, M. (2008). Direct recovery of infectious pestivirus from a full-length RT-PCR amplicon. Journal of Virological Methods, 149: 330-333.
[4]
Rumenapf, T., Meyers, G., Stark, R. and Thiel, H. J. (1991). Molecular characterization of hog cholera virus. Archives of Virology, Supplementum, 3: 7-18.
[5]
Fulton, R. W., Briggs, R. E., Ridpath, J. F., Saliki, J. T., Confer, A. W., Payton, M. E., Duff, G. C., Step, D. L. and Walker, D. A. (2005). Transmission of bovine viral diarrhoea virus 1b to susceptible and vaccinated calves by exposure to persistently infected calves. Canadian Journal of Veterinary Research, 69: 161-169.
[6]
Berriatua, E., Barandika, J. F., Aduriz, G., Hurtado, A., Estevez, L., Atxaerandio, R. and Garcia-Perez, A. L. (2006). Flock-prevalence of border disease virus infection in Basque dairy-sheep estimated by bulk-tank milk analysis. Veterinary Microbiology, 118: 37-46.
[7]
Dubois, E., Russo, P., Prigent, M. and Thiery, R. (2008). Genetic characterization of ovine pestiviruses isolated in France, between 1985 and 2006. Veterinary Microbiology, 130: 69-79.
[8]
Moennig, V., Frey, H. R., Liebler, E., Pohlenz, J. and Liess, B. (1990). Reproduction of mucosal disease with cytopathogenic bovine viral diarrhea virus selected in vitro. The Veterinary Record, 127: 200-203.
[9]
Hughes, L. E., Kershaw, G. F. and Shaw, I. G. (1959). "B" or Border Disease: An undescribed disease of sheep. The Veterinary Record, 71: 313-317.
[10]
Sandvik, T. (2005). Selection and use of laboratory diagnostic assays in BVD control programmes. Preventive Veterinary Medicine, 72: 3-16.
[11]
Yavru, S., Kale, M., Gulay, M. S., Yapici, O., Bulut, O. and Ata, A. (2013). Effects of bovine viral diarrhoea virus on the fertility of cows. Acta Veterinaria Hungarica, 61: 2. DOI 10.1556/AVet.2013.008.
[12]
Marco, I., Rosell, R., Cabezon, O., Beneria, M., Mentaberre, G., Casas, E., Hurtado, A., Lopez-Olvera, J. R. and Lavin, S. (2009). Serologic and virologic investigations into pestivirus infection in wild and domestic ruminants in the Pyrenees (NE Spain). Research in Veterinary Science, 87: 149-153.
[13]
Xia, H., Vijayaraghavan, B., Belak, S. and Liu, L. (2011). Detection and identification of the atypical bovine pestiviruses in commercial foetal bovine serum batches. PloS One, 6: e28553.
[14]
Hurtado, A., Sanchez, I., Bastida, F., Minguijon, E., Juste, R. A. and Garcia-Perez, A. L. (2009). Detection and quantification of pestivirus in experimentally infected pregnant ewes and their progeny. Virology Journal, 6: 189-196.
[15]
Kittelberger, R. and Pigott, C. (2008). The use of pestivirus antigen ELISA currently available for the detection of hairy shaker disease/border disease virus in sheep. New Zealand Veterinary Journal, 56: 343-344.
[16]
Gardiner, A. C., Nettleton, P. F. and Barlow, R. M. (1983). Virology and immunology of a spontaneous and experimental mucosal disease-like syndrome in sheep recovered from clinical border disease. Journal of Comparative Pathology, 93: 463-469.
[17]
Monies, R. J., Paton, D. J. and Vilcek, S. (2004). Mucosal disease-like lesions in sheep infected with Border disease virus. The Veterinary Record, 155: 765-769.
[18]
Hyera, J. M. K., Liess, B. and Frey, H. R. (1987). A direct neutralizing peroxidase-linked antibody assay for detection and titration of antibodies to bovine viral diarrhea virus. Journal of Veterinary Medicine B, 34: 227-239.
[19]
Vilcek, S. and Paton, D. J. (2000). A RT-PCR assay for the rapid recognition of border disease virus. Veterinary Research, 31: 437-445.
[20]
Garcia-Perez, A. L., Minguijon, E., Jesus, F. B., Aduriz, G., Povedano, I., Juste, R. A. and Hurtado, A. (2009). Detection of Border disease virus in fetuses, stillbirths, and newborn lambs from natural and experimental infections. Journal of Veterinary Diagnostic Investigation, 21: 331-337.
[21]
Burgu, I., Ozturk, F., Akca, Y., Toker, A., Frey, H. R. and Liess, B. (1987). Investigations on the occurence and impact of bovine viral diarrhea (BVD) virus infections in sheep in Turkey. Dtsch Tierarztl Wochenschr, 94: 292-294.
[22]
Simsek, A., Yavru, S. and Ozturk, F. (1997). The serologic survey on bovine viral diarrhea virus infections in sheep in Konya. Veterinarium, 8: 45-47.
[23]
Cokcaliskan, C. (2000). Pestivirus infections of pregnant sheep and their fetuses. Ankara University Graduate School of Health Sciences. Doctorate Thesis, Ankara, Turkey.
[24]
Hasircioglu, S., Kale, M. and Acar, A. (2009). Investigation of pestivirus infections in aborted sheep and goats in Burdur region. Kafkas Universitesi Veteriner Fakültesi Dergisi, 15: 163-167.
[25]
Burgu, I., Akca, Y., Alkan, F., Ozkul, A., Karaoglu, T., Bilge-Dagalp, S., Oguzoglu, T. C. and Yesilbag, K. (2001). The serological and virological investigations and pathogenesis of BVDV infection in sheep during pre- and post-partum periods. Turkish Journal of Veterinary and Animal Sciences, 25: 551-557.
[26]
O’Neill, G. R., O’connor, M. and O’rolly, J. P. (2004). A survey of antibodies to pestivirus in sheep in the Republic of Ireland. Irish Veterinary Journal, 57: 525-530.
[27]
Ataseven, V. S., Ataseven, L., Tan, T., Babur, C. and Oguzoglu, T. C. (2006). Seropositivity of agents causing abortion in local goat breeds in Eastern and South-eastern Anatolia, Turkey. Revue de Medecine Veterinaire, 157: 545-550.
[28]
Okur-Gumusova, S., Yazici, Z. and Albayrak, H. (2006). Pestivirus seroprevalence in sheep populations from inland and coastal zones of Turkey. Revue de Medecine Veterinaire, 157: 595-598.
[29]
Oguzoglu, T. C., Tan, M. T., Toplu, N., Demir, A. B., Dagalp, S. B., Karaoglu, T., Ozkul, A., Alkan, F., Burgu, I., Haas, L. and Greiser-Wilke, I. (2009). Border disease virus (BDV) infections of small ruminants in Turkey: A new BDV subgroup? Veterinary Microbiology, 135: 374-379.
[30]
Valdazo-Gonzalez, B., Alvarez-Martinez, M. and Greiser-Wilke, I. (2006). Genetic typing and prevalence of Border disease virus (BDV) in small ruminant flocks in Spain. Veterinary Microbiology, 117: 141-153.
[31]
Berriatua, E., Barandika, J., Aduriz, G., Atxaerandio, R., Garrido, J. and Garcia-Perez, A. L. (2004). Age-spesific seroprevalence of border disease virus and presence of persistently infected sheep in Basque dairy-sheep flocks. The Veterinary Journal, 168: 336-342.
[32]
Willoughby, K., Valdazo-Gonzalez, B., Maley, M., Gilray, J. and Nettleton, P. F. (2006). Development of a real time RT-PCR to detect and type ovine pestiviruses. Journal of Virological Methods, 132: 187-194.
[33]
Fenton, A., Sinclair, J. A., Entrican, G., Herring, J. A., Malloy, C. and Nettleton, P. F. (1991). A monoclonal antibody capture ELISA to detect antibody to border disease virus in sheep serum. Veterinary Microbiology, 28: 327-333.
ADDRESS
Science Publishing Group
1 Rockefeller Plaza,
10th and 11th Floors,
New York, NY 10020
U.S.A.
Tel: (001)347-983-5186