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An Easy-to-Use Colorimetric Enzymatic Test-System for Organophosphorus Insecticides Detection in Olive Oil

Received: 26 September 2014    Accepted: 30 September 2014    Published: 17 October 2014
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Abstract

Immobilization of a low amount of electric eel acetylcholinesterase on microtiter plates by bioencapsulation in a sol-gel composite led to the preparation of a simple colorimetric enzymatic-assay for pesticides detection in olive oil. This bioassay has been used to carry out inhibition studies with three pesticides mainly used in the treatment of olive trees: Malaoxon, Methidathion-oxon and Omethoate, and tested using standard solutions and real samples of olive oil. The developed bio-tools showed a good reproducibility and stability as well as good analytical performances with limits of detection at least 10 times less than the maximum residue limit tolerated by international regulations. The obtained LODs were as low as 10-9 M for the widely used pesticide Malaoxon, 10-8 M for Methidathion oxon and 10-7 M for Omethoate. The developed test was designed for fast detection of the three target insecticides in such a complex matrix as olive oil without a laborious pre-treatment and after a simple liquid-liquid extraction, which demonstrate the potentiality of this technique to be used for commercial purpose.

DOI 10.11648/j.si.s.2014020601.12
Published in Science Innovation (Volume 2, Issue 6-1, December 2014)

This article belongs to the Special Issue Biosensors: Innovative Technique for Food Safety and Environmental Monitoring

Page(s) 5-9
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Electric Eel Acetylcholinesterase, Bioassays, Organophosphorus Insecticides, Olive Oil, Colorimetric Test, Sol-Gel

References
[1] Garcıa-Reyes, J. F., Ferrer, C, Gomez-Ramos, M. J., Molina-Diaz, A, Fernandez-Alba, A. R. Determination of pesticide residues in olive oil and olives. Trend. Anal. Chem., 26 (2007) 239-251.
[2] Luchetti, F. Importance and future of olive oil in the world market e an introduction to olive oil. Eur. J. Lipid Sci. Technol., 104 (2002) 559-563.
[3] Lentza-Rizos, C., & Avramides, E. J. Pesticide residues in olive oil. Arch. Environ. Contam. Toxicol., 141, (1995), 111-134.
[4] Istamboulie, G., Andreescu, S., Marty, J. L., & Noguer, T. Highly sensitive detection of organophosphorus insecticides using magnetic microbeads and genetically engineered acetylcholinesterase. Biosens. Bioelectron., 23 (2007) 506-512.
[5] Ellman, G.L., Courtney, K.D., Andres, V., Featherstone, R.M. A new and rapid colorimetric determination of acetylcholi-nesterase activity. Biochem. Pharmacol. 7 (1961) 88–92.
[6] Ebelmen, J. J. Sur les éthers siliciques. C.R. Acad. Sci., 19. (1844) 398-400.
[7] Andreescu, S., Barthelmebs, L., Marty, J-L. Anal. Immobilization of acetylcholinesterase on screen-printed electrodes: comparative study between three immobilization methods and applications to the detection of organophosphorus insecticides. Anal. Chim. Acta., 464 (2002) 171-180.
[8] Alvarez, G. S., Desimone, M. F., Diaz, L. E. Immobilization of bacteria in silica matrices using citric acid in the sol-gel process. Applied Microbiol. Biotechnol. 73 (2007) 1059–1064.
[9] Sassolas, A., Blum, L.-J., & Leca-Bouvier, B.-D. Immobilization strategies to develop enzymatic biosensors. Biotechnol. Adv., 30 (2012) 489-511.
[10] Hayat, A., Barthelmebs, L., Marty, J.-L. A simple colorimet-ric enzymatic-assay for okadaic acid detection based on the immobilization of protein phosphatase 2A in sol-gel. Appl. Biochem. Biotech. 166 (2012), 47-56.
[11] Bavcon Kralj, M., Treb se, P., & Franko, M. Oxidation as a pre-step in determination of organophosphorus compounds by the AChE-TLS bioassay. Acta Chim. Slov., 53 (2006) 43-51.
[12] Ben Oujji, N., Bakas, I., Istamboulié, G., Ait-Ichou, I., Ait-Addi, E., Rouillon, R., Noguer, T. A Simple Colorimetric Enzymatic-Assay, based on immobilization of acetylcholi-nesterase by adsorption, for sensitive detection of organo-phosphorus insecticides in olive oil. Food Control. 46, 2014, 75-80.
[13] Ben Oujji, N., Bakas, I., Istamboulié, G., Ait-Ichou, I., Ait-Addi, E., Rouillon, R., Noguer, T. Sol-gel immobilization of acetylcholinesterase for the determination of orga-nophosphate pesticides in olive oil with biosensors. Food Control, 30 (2013) 657-661.
Author Information
  • University of Perpignan, IMAGES EA4218, 52 Av. Paul Alduy, 66860 Perpignan Cedex, France; University Ibn Zohr, Photocatalysis and Environment Team, Chemistry Department/Faculty of Science, Agadir, Morocco

  • University of Perpignan, IMAGES EA4218, 52 Av. Paul Alduy, 66860 Perpignan Cedex, France; University Ibn Zohr, Photocatalysis and Environment Team, Chemistry Department/Faculty of Science, Agadir, Morocco

  • University of Perpignan, IMAGES EA4218, 52 Av. Paul Alduy, 66860 Perpignan Cedex, France

  • University Ibn Zohr, Photocatalysis and Environment Team, Chemistry Department/Faculty of Science, Agadir, Morocco

  • University Ibn Zohr, Photocatalysis and Environment Team, Chemistry Department/Faculty of Science, Agadir, Morocco

  • University of Perpignan, IMAGES EA4218, 52 Av. Paul Alduy, 66860 Perpignan Cedex, France

  • University of Perpignan, IMAGES EA4218, 52 Av. Paul Alduy, 66860 Perpignan Cedex, France

Cite This Article
  • APA Style

    Najwa Ben Oujji, Idriss Bakas, Georges Istamboulie, Ihya Ait-Ichou, Elhabib Ait-Addi, et al. (2014). An Easy-to-Use Colorimetric Enzymatic Test-System for Organophosphorus Insecticides Detection in Olive Oil. Science Innovation, 2(6-1), 5-9. https://doi.org/10.11648/j.si.s.2014020601.12

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    ACS Style

    Najwa Ben Oujji; Idriss Bakas; Georges Istamboulie; Ihya Ait-Ichou; Elhabib Ait-Addi, et al. An Easy-to-Use Colorimetric Enzymatic Test-System for Organophosphorus Insecticides Detection in Olive Oil. Sci. Innov. 2014, 2(6-1), 5-9. doi: 10.11648/j.si.s.2014020601.12

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    AMA Style

    Najwa Ben Oujji, Idriss Bakas, Georges Istamboulie, Ihya Ait-Ichou, Elhabib Ait-Addi, et al. An Easy-to-Use Colorimetric Enzymatic Test-System for Organophosphorus Insecticides Detection in Olive Oil. Sci Innov. 2014;2(6-1):5-9. doi: 10.11648/j.si.s.2014020601.12

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  • @article{10.11648/j.si.s.2014020601.12,
      author = {Najwa Ben Oujji and Idriss Bakas and Georges Istamboulie and Ihya Ait-Ichou and Elhabib Ait-Addi and Régis Rouillon and Thierry Noguer},
      title = {An Easy-to-Use Colorimetric Enzymatic Test-System for Organophosphorus Insecticides Detection in Olive Oil},
      journal = {Science Innovation},
      volume = {2},
      number = {6-1},
      pages = {5-9},
      doi = {10.11648/j.si.s.2014020601.12},
      url = {https://doi.org/10.11648/j.si.s.2014020601.12},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.si.s.2014020601.12},
      abstract = {Immobilization of a low amount of electric eel acetylcholinesterase on microtiter plates by bioencapsulation in a sol-gel composite led to the preparation of a simple colorimetric enzymatic-assay for pesticides detection in olive oil. This bioassay has been used to carry out inhibition studies with three pesticides mainly used in the treatment of olive trees: Malaoxon, Methidathion-oxon and Omethoate, and tested using standard solutions and real samples of olive oil. The developed bio-tools showed a good reproducibility and stability as well as good analytical performances with limits of detection at least 10 times less than the maximum residue limit tolerated by international regulations. The obtained LODs were as low as 10-9 M for the widely used pesticide Malaoxon, 10-8 M for Methidathion oxon and 10-7 M for Omethoate. The developed test was designed for fast detection of the three target insecticides in such a complex matrix as olive oil without a laborious pre-treatment and after a simple liquid-liquid extraction, which demonstrate the potentiality of this technique to be used for commercial purpose.},
     year = {2014}
    }
    

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  • TY  - JOUR
    T1  - An Easy-to-Use Colorimetric Enzymatic Test-System for Organophosphorus Insecticides Detection in Olive Oil
    AU  - Najwa Ben Oujji
    AU  - Idriss Bakas
    AU  - Georges Istamboulie
    AU  - Ihya Ait-Ichou
    AU  - Elhabib Ait-Addi
    AU  - Régis Rouillon
    AU  - Thierry Noguer
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    DO  - 10.11648/j.si.s.2014020601.12
    T2  - Science Innovation
    JF  - Science Innovation
    JO  - Science Innovation
    SP  - 5
    EP  - 9
    PB  - Science Publishing Group
    SN  - 2328-787X
    UR  - https://doi.org/10.11648/j.si.s.2014020601.12
    AB  - Immobilization of a low amount of electric eel acetylcholinesterase on microtiter plates by bioencapsulation in a sol-gel composite led to the preparation of a simple colorimetric enzymatic-assay for pesticides detection in olive oil. This bioassay has been used to carry out inhibition studies with three pesticides mainly used in the treatment of olive trees: Malaoxon, Methidathion-oxon and Omethoate, and tested using standard solutions and real samples of olive oil. The developed bio-tools showed a good reproducibility and stability as well as good analytical performances with limits of detection at least 10 times less than the maximum residue limit tolerated by international regulations. The obtained LODs were as low as 10-9 M for the widely used pesticide Malaoxon, 10-8 M for Methidathion oxon and 10-7 M for Omethoate. The developed test was designed for fast detection of the three target insecticides in such a complex matrix as olive oil without a laborious pre-treatment and after a simple liquid-liquid extraction, which demonstrate the potentiality of this technique to be used for commercial purpose.
    VL  - 2
    IS  - 6-1
    ER  - 

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