Germination and Root Nodule Formation of Soybean (Glycine max (L.) Merr.) in Ridomil and Chlorpyriphos Treated Soil
American Journal of Environmental Protection
Volume 4, Issue 1, February 2015, Pages: 17-22
Received: Dec. 19, 2014;
Accepted: Dec. 27, 2014;
Published: Jan. 14, 2015
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Ibiang Young Bassey, Department of Genetics and Biotechnology, University of Calabar, PMB 1115, Calabar, Nigeria
Ekanem Bassey Effiong, Department of Genetics and Biotechnology, University of Calabar, PMB 1115, Calabar, Nigeria; Department of Science Technology, Akwa Ibom State Polytechnic Ikot Osurua, PMB 1200, Ikot Ekpene, Nigeria
Usanga Daniel Archibong, Department of Genetics and Biotechnology, University of Calabar, PMB 1115, Calabar, Nigeria
Williams Uduak Ita, Department of Genetics and Biotechnology, University of Calabar, PMB 1115, Calabar, Nigeria
Germination and root nodule formation of soybean (Glycine max) was evaluated in soil treated with Ridomil (R), Chlorpyriphos (CH), and a Combination of both (COM); using a completely randomized design, and a 3×3 factorial arrangement. Factor 1 was the pesticide type with 3 levels - R, CH, and COM; while factor 2 was the contamination rate (v/wt) with 3 levels - 0% (control), 2%, and 4%. Aqueous pesticides preparations (as recommended by manufacturers) were applied once, before seeds were planted. More than Ridomil and Combination, significantly (P<0.05) diminished percentage germination due to Chlorpyriphos was observed at 2, 3, and 4 weeks post application. The control had significantly higher germination than 2% and 4% groups, up until the 5th week. The Chlorpyriphos and pesticide Combination groups both had significantly lower root nodule number/plant, than Ridomil. Root nodule number/plant, and nodule biomass/plant were significantly reduced due to pesticide stress at 2% and 4%. Pesticides application at 2% and 4% negatively affected germination and root nodule formation; but there was no indication of an enhanced effect due to pesticides combination.
Ibiang Young Bassey,
Ekanem Bassey Effiong,
Usanga Daniel Archibong,
Williams Uduak Ita,
Germination and Root Nodule Formation of Soybean (Glycine max (L.) Merr.) in Ridomil and Chlorpyriphos Treated Soil, American Journal of Environmental Protection.
Vol. 4, No. 1,
2015, pp. 17-22.
Ashraf M., Harris P.J.C. 2004. Potential biochemical indicators of salinity tolerance in plants. Plant Science, 166, pp 3-16
Gürsoy M., Balkan A., Ulukan H. 2012. Ecophysiological responses to stress in plants: a general approach. Pakistan Journal of Biological Science, 15(11), pp 506-516, doi:10.3923/pjbs.2012.506.516
Singh T.B., Mukhopadhayay S.K., Sar T.K., Ganguly S. 2012 Acetamiprid Induces Toxicity in Mice under Experimental Conditions with Prominent Effect on the Hematobiochemical Parameters. J. Drug Metab Toxicol, 3:134. doi:10.4172/2157-7609.1000134
Tijani, A. A. 2006. Pesticide Use Practices and Safety Issues: The case of Cocoa farmers in Ondo State Nigeria. J. Hum. Eco., 19, pp 183-190.
Asogwa, E.U., Dongo, L.N. 2009. Problems associated with pesticide usage and application in Nigerian cocoa production: A review. African Journal of Agricultural Research, 4, pp 675-683.
Ibiang, Y.B., Ekaluo U.B., Nta A.I., Ikpeme E.V., Ekanem B.E., Erem, F.A. 2013. Effect Of Deltamethrin And Ridomil On Serum Biochemical Parameters In The Rat (Rattus Norvegicus). European Journal of Toxicological Sciences, 2013:5.
Römbke J., Schmelz R.M., Knaebe S. 2009. Field studies for the assessment of with soil mesofauna, in particular enchytraeids, mites and nematodes: Design and first results. Soil Organisms, 81(2), pp 237-264
Toscano N.C., Sances F.V., Johnson M.W., Laprel F. 1982. The effect of various pesticides on lettuce physiology and yield. Journal of Economical Entomology, 75, pp 738-741
Jones V.P., Toscano N.C., Johnson M.W., Welter S.C., Youngman R.R. 1986. Pesticide effect on plant physiology: Integration into a pest management program. Bulletin of the Entomological Society of America, 32, pp 103-109.
Dunfield K.E., Siciliano S.D., Germida J.J. 2000. The fungicides thiram and captan affect the phenotypic characteristics of Rhizobium leguminosarum strain C1 as determined by FAME and Biolog analyses. Biology and Fertility of Soils, 31 (3), pp 303-309
Ahemad M. (2011). A Comparative analysis of Tebuconazole mediated phytotoxicity to legumes. Journal of Environmental Science and Technology 4(6), pp 630-637 10.3923/jest.2011.630.637
Rasool, N., Reshi, Z. 2010. Effect of the fungicide Mancozeb at different application rates on enzyme activities in a silt loam soil of the Kashmir Himalaya India. Tropical Ecology, 51(2), pp 199-205
Goswami M.R., Pati U.K., Chowdhury A., Mukhopadhyay A. 2013. Studies on the effect of cypermethrin on soil microbial biomass and its activity in an alluvial soil. International Journal of Agriculture and Food Science, 3(1), pp 1-9
Raven P.H., Johnson G.B., Losos J.B., Singer S.R. 2005. BIOLOGY, 7th Edn, McGraw Hill, New York, p 741.
Hodgson, E., Mailman B. R., Chambers J. E. (Eds) 1998. Dictionary of Toxicology, 2nd ed, Macmillan Reference Ltd, London, pp 186-190.
Nta, A.I., Ibiang Y.B., Uyoh E.A., Edu N.E., Ekanem B.E., John Q.E. 2013. Insect pest damage to leaves of cowpea (Vigna unguiculata L. Walp): comparative effects of aqueous extracts of Piper guineensis, Allium sativum and Myristica fragrans. IOSR Journal of Environmental Science, Toxicology and Food Technology, 3 (2), pp 17-20
O.M. Agbogidi (2011). Susceptibility of five cultivars of soyabean (Glycine max (L.) Merr) seeds to spent engine oil. International Journal of Science and Nature, 2(3), pp 447-451
Dubey K. K., Fulekar M. H. 2011. Effect of pesticides on the seed germination of Cenchrus setigerus and Pennisetum pedicellatum as monocropping and co-cropping system: implications for rhizospheric bioremediation. Romanian Biotechnological Letters, 16 (1), pp 5909-5918.
Siddiqui Z.S., Ahmed S. 2006. Combined effects of pesticide on growth and nutrient composition of soybean plants. Pak. J. of Botany, 38, pp721-733
Einhellig, F.A., Muth M.S., Schon M.K. 1985. Effects of allelochemicals on plant-water relationship. In: Thomson A.C. (Ed.) The chemistry of Allelopathy. American Chemical Society, Washington.
Macias, F.A., Galindo J.C.G., Massanot G.M. 1992. Potential allelopathic activity of several sesquterpene lactone models. Phytochem., 31, pp 1969-1777.
Mersie, W., Singh, M. 1993. Phenolic acids affect photosynthesis and protein synthesis by isolated leaf cells of velvet-leaf. J. Chem. Ecol., 19, pp 1293-1301.
Ahemad M. and Khan M. S. 2011. Comparative study of the growth parameters of legumes grown in fipronil-stressed soils. Eurasia J. Biosci. 5, pp 29-36.doi:10.5053/ejobios.2011.5.0.4
Anderson A., Baldock J. A., Rogers S. L., Belloti W., Gill G. 2004. Influence of chlorsulfuron on rhizobial growth, nodule formation, and nitrogen fixation with chickpea. Australian Journal of Agriculture Research, 55, pp 1059-1070.
Kimmel, E.C., Casida, J.E., Ruzo, L.O. 1986. Formamide Insecticide and chimoacetanide herbicides; disubstituted Anilines and Nitro benzenes as mammalian metabultes and bacterial metagens. Journal of Agriculture and Food Chemistry, 34, pp 157-161.
Sing, B.K., Walker A., Wright D.J. 2002. Degradation of chlorpyrifos, fenamiphos and chlorothelonil alone and in combination and their effects on microbial activity. Environmental Toxicology and Chemistry, 21, pp 2600-2605.
Glesy J.P., Solomon K.R., Mackay D., Anderson J. 2014. Evaluation of evidence that the organophosphorus insecticide chlorpyrifos is a potential persistent organic pollutant (POP) or persistent, bioaccumulative, and toxic (PBT). Environmental Sciences Europe, 26:29. doi:10.1186/s12302-014-0029-y