Potential Impact of Some Soil Borne Fungi on Biodegradation of Some Organophosphorous Nematicides
American Journal of Environmental Protection
Volume 3, Issue 6, December 2014, Pages: 299-304
Received: Nov. 4, 2014;
Accepted: Nov. 24, 2014;
Published: Nov. 27, 2014
Views 2775 Downloads 179
Tamer M. A. Thabit, Central Agric, Pesticides Lab. (CAPL) Agric, Research Center, Giza, Egypt; Central Lab. of Research, Grain Silos & Flour Mills Org. (GSFMO) B.O. Box 3402Riyadh 11471, KSA
Medhat A. H. El-Naggar, Central Lab. of Research, Grain Silos & Flour Mills Org. (GSFMO) B.O. Box 3402Riyadh 11471, KSA; Plant Pathology Research Institute, Agric, Research Center, Giza, Egypt
Organophosphorous nematicides are highly toxic pesticides used to control nematodes in agriculture soil. An in vitro Biodegradation study was conducted to determine the biodegradability of, ethoprophos, fenamiphos and triazophos nematicides, using fungi strains isolated from sandy agriculture soil under date palm trees. Five fungi strains labeled as S1 (Fusarium oxysporum), S2(Aspergillus flavus), S3 (Aspergillus fumigatus), S4 (Fusarium moniliforme) and S5 (Trichothecium roseum) were isolated and identified, then incubated with nematicides at successive intervals untill 45 days in liquid medium paralleled with control samples. Recovery rates were performed at two levels 0.1 and 1 mg kg-1, values were over 90% for all nematicides. Limit of detection values (LOD) were 0.010, 0.012 and 0.011 mg kg-1 and limit of quantitation values (LOQ) were 0.033, 0.040 and 0.036 mg kg-1 respectively. Data indicated that S1 (Fusarium oxysporum) and S2 (Aspergillus flavus) accelerated the degradation rate of all mentioned nematicise, and S2 had the highest impact more than S1, while the other strains had no significant effect. Half-life values (RL50) for nematicides with S1 were 18.15, 16.65 and 15.24 days, respectively, and with S2 were 10.35, 13.87 and 11.18 days, respectively, while control values were 26.30, 24.28 and 26.70 days, respectively.
Tamer M. A. Thabit,
Medhat A. H. El-Naggar,
Potential Impact of Some Soil Borne Fungi on Biodegradation of Some Organophosphorous Nematicides, American Journal of Environmental Protection.
Vol. 3, No. 6,
2014, pp. 299-304.
Barnett, H.L. (1960): Illustrated Genera of Imperfect Fungi. – In: Burgess Publishing Company, Minneapolis, Minnesota.
Boopathy, R. (2000): Factors limiting bioremediation technologies. – Bioresource Technology 74: 63-67.
Carlile, M. J., Watkinson, S. C., Gooday, G. W. (2001): The Fungi. – Elsevier Academic Press, London.
Christian, G. (1990): HPLC tips and tricks. – Iden Press, Oxford.
Gan, J., Koskinen, W.C. (1998): Pesticide fate and behaviour in soil at elevated concentrations. – In: Kearney, P.C. (ed.) Pesticide Remediation in Soils and Water. John Wiley & Sons, Chichester.
Geraldo, M. R. F., Tessmann, D. J., Kemmelmeier, C. (2006): Production of mycotoxins by Fusarium graminearum isolated from small cereals (wheat, triticale and barley) affected with scab disease in southern Brazil. – Brazilian Journal of Microbiology 37: 58-63.
Harms, H., Schlosser, D., Wick, L.Y. (2011): Untapped potential: exploiting fungi in bioremediation of hazardous chemicals. – Natural Reviews Microbiology 9: 177-192.
Hasan, H. A. (1999): Fungal utilization of organophosphate pesticides and degradation by Aspergillus flavus and A. sydowii in soil. – Folia Microbiologica 44(1): 77-84.
Hawksworth, D. L. (1991): The fungal dimension of biodiversity: magnitude, significance, and conservation. – Mycological Research. 95: 641-655.
Hawksworth, D. L. (2001): The magnitude of fungal diversity: the 1.5 million species estimate revisited. – Mycological Research 105: 1422-1432.
ICH (1996): Validation of Analytical Procedure. International Conference on Harmonisation (ICH) of Technical Requirements for Registration of Pharmaceuticals for Human Use. – Methodology, Q2B, Geneva.
Juwarkar, A., Singh, S., Mudhoo, A. (2010):. A comprehensive overview of elements in bioremediation. – Reviews in Environmental Science and Biotechnology 9: 215-288.
Koehler, P. E., Hanlin, R. T., Beraha, L. (1975): Production of Aflatoxins B1 and G1 by Aspergillus flavus and Aspergillus parasiticus Isolated from Market Pecans. – American Society for Microbiology 30(4): 581-583.
Korn-Wendisch, F., Kutzner, H.J. (1992): The family Streptomycetaceae. In the Procaryotes. – In: Balows, A., Truper H.G, Dworkin, M., Harder, W., Schleifer, K.H. (eds.). Springer-Verlag, New York.
Kuster, E., Williams, S.T. (1964): Selection of media for isolation of streptomycetes. – Nature 202: 928-929.
Lechevalier, M.P., Lechevalier, H. (1970): Chemical composition as a criterion in the classification of aerobic actinomycetes. – International journal of systematic bacteriology 20: 435-443.
Lopez-Blanco, C., Gomez-Alvarez, S., Rey-Garrote, M., Cancho-Grande, B., Simal-Gandara, J. (2006): Determination of pesticides by solid phase extraction followed by gas chromatography with nitrogen-phosphorous detection in natural water and comparison with solvent drop microextraction. – Analytical and Bioanalytical Chemistry 384: 1002-1006.
Moye, H. A., Malagodi, M. H., Yoh, J., Leibee, G. L., Ku, C. C., Wislocki, P. G. (1987): Residues of avermectin B1a rotational crop and soils following soil treatment with (14C) avermectin B1a. – Journal of Agriculture and Food Chemistry 35: 859-864.
Nyakundi, W. O., Magoma, G., Ochora, J., Nyende, A. B. (2011): Biodegradation of diazinon and methomyl pesticides by white rot fungi from selected horticultural farms in rift valley and central kenya. – Journal of Applied Technology in Environmental Sanitation 1 (2): 107-124.
Pointing, S. (2001): Feasibility of bioremediation by white-rot fungi. – Applied Microbiology and Biotechnology 57: 20-33.
Salama, A. K., Al-Mihanna, A. A., Abdalla, M. Y.(1999): Microbial degradation of pirimiphos-methyl and carbaryl by culture of two soil fungi. – Journal of King Saud University of Agricultural Science 11(1): 25-32.
Schoefs, O., Perrier, M., Samson, R. (2004): Estimation of contaminant depletion in unsaturated soils using a reduced-order biodegradation model and carbon dioxide measurement. – Applied Microbiology and Biotechnology 64: 256-61.
Singh, H. (2006): Mycoremediation: Fungal bioremediation. – John Wi ley & Sons Inc, New Jersey.
Smalla, K., Wachtendorf, U., Heuer, H. (1998): Analysis of BIOLOG GN substrate utilization patterns by microbial communities. – Applied and Environmental Microbiology 64: 1220-1225.
Snedecor, G. W., Cochran, W. G. (1967): Statistical methods 6th ed. – Press Ames, Iowa state University, Iowa.
Stefanowicz, A. (2006): The Biolog Plates Technique as a Tool in Ecological Studies of Microbial Communities. – Polish Journal of Environmental Studies 15(5): 669-676.
Stubblefield, R. D., Greer, J. I., Shotwell, O. L. (1988): Liquid chromatographic method for determination of citreoviridin in corn and rice. – Journal of AOAC 71: 721-724.
Subramanian, C.V. (1971): Hyphomycetes. – Indian Council of Agricultural Research, New Delhi.
Timme, G., Frehse, H., (1980): Statistical interpretation and graphic representation of the degradation behaviour of pesticide residues. – Pflanzenschutz Nachrichten Bayer 33: 47-60.
Tomlin, C. D. S., (2006): The Pesticide Manual. – 14th Ed. World Compendium, British Crop Protection Council (BCPC), Alton, Hampshire.
Tousson, T.A, Nelson, P.E. (1976): A Pictorial Guide to the Identification of Fusarium Species according to the taxonomic system of Snyder and Hansen, 2 nd ed. – The Pennsylvania State University Press, University Park, Pennsylvania.
Zhang, J., Chiao, C. (2002): Novel Approaches for remediation of pesticide pollutants. – International Journal Environment and Pollution 18(5): 423-433.