Incident Monitoring of Glyphosate and Aminomethylphosphonic Acid in Natural Waters – Experimental Set-up and Validation
International Journal of Environmental Monitoring and Analysis
Volume 4, Issue 3, June 2016, Pages: 75-81
Received: Feb. 27, 2016; Accepted: Mar. 8, 2016; Published: Apr. 20, 2016
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Britta Jahnke, Institute of Coastal Research / Molecular Recognition and Separation, Helmholtz-Zentrum Geesthacht, Geesthacht, Germany
Bernd Niemeyer, Institute of Coastal Research / Molecular Recognition and Separation, Helmholtz-Zentrum Geesthacht, Geesthacht, Germany; Institute of Thermodynamics, Helmut-Schmidt-University / University of the Federal Armed Forces Hamburg, Hamburg, Germany
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A measuring system based on sequential injection analysis (SIA) for the detection of the herbicide glyphosate and its main metabolite aminomethylphosphonic acid (AMPA) in natural waters is presented. The system is automated to enable an unattended monitoring of the analytes. Due to its compact manifold it can easily be integrated into existing observing systems or could be used on board of research vessels. Here we show the experimental setup and the results of the system’s performance during experimental periods of 20 hours in the laboratory as well as in an observing station situated at the Elbe river in Hamburg, Germany. An incident with elevated glyphosate and AMPA concentrations was simulated by injecting spiked river water samples. The results show a good stability of the system over the experimental period.
Glyphosate, Roundup, AMPA, Sequential Injection Analysis, SIA, Phosphororganic Analysis
To cite this article
Britta Jahnke, Bernd Niemeyer, Incident Monitoring of Glyphosate and Aminomethylphosphonic Acid in Natural Waters – Experimental Set-up and Validation, International Journal of Environmental Monitoring and Analysis. Vol. 4, No. 3, 2016, pp. 75-81. doi: 10.11648/j.ijema.20160403.12
Copyright © 2016 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License ( which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Glyphosate herbicide sales boom powers global biotech industry. Sustainable pulse. 21. 08. 2014. (accessed 16.06.2015).
Deutscher Bundestag. Drucksache 16/13993. Einsatz von Pestiziden auf Strecken der deutschen Bahn. 07. 09. 2009.
S. Daouk, L. F. de Alencastro, H. R. Pfeifer. The herbicide glyphosate and its metabolite AMPA in the Lavaux vineyard area, western Switzerland: Proof of widespread export to surface waters. Part II: The role of infiltration and surface runoff. J Environ Sci Heal B. vol. 48, pp. 725-736, 2013.
H. Horth. Monitoring results for surface water and groundwater. European glyphosate environmental information source. 2010.
T. Tang, W. Boenne, N. Desmet, P. Seuntjens, J. Bronders, A. van Griensven. Quantification and characterization of glyphosate use and loss in a residential area. Sci Total Environ. vol. 517, pp. 207-214, 2015.
A. E. Rosenbom, P. Olsen, F. Plauborg, R. Grant, R. K. Juhler, W. Brusch, J. Kjaer. Pesticide leaching through sandy and loamy fields - long-term lessons learnt from the Danish pesticide leaching assessment programme. Environ Pollut. vol. 201, pp. 75-90, 2015.
L. Puertolas, J. Damasio, C. Barata, A. M. V. M. Soares, N. Prat. Evaluation of side-effects of glyphosate mediated control of giant reed (Arundo donax) on the structure and function of a nearby Mediterranean river ecosystem. Environ Res. vol. 110, pp. 556-564, 2010.
K. R. Solomon, D. G. Thompson. Ecological risk assessment for aquatic organisms from over-water uses of glyphosate. J Toxicol Env Heal B. vol. 6, pp. 289-324, 2003.
K. Z. Guyton, D. Loomis, Y. Grosse, F. El Ghissassi, L. Benbrahim-Tallaa, N. Guha, C. Scoccianti, H. Mattock, K. Straif. Carcinogenicity of tetrachlorvinphos, parathion, malathion, diazinon, and glyphosate. Lancet Oncol. vol. 16, pp. 490-491, 2015.
Fragen und Antworten zu Glyphosat. Bundesministerium für Ernährung und Landwirtschaft. (accessed 28. 12. 2015).
K. M. A. Holvoet, P. Seuntjens, P. A. Vanrolleghem. Monitoring and modeling pesticide fate in surface waters at the catchment scale. Ecol Model. vol. 209, pp. 53-64, 2007.
I. D. McKelvie, S. D. Kolev, P. J. Worsfold. More with less: Advances in flow and paper-based monitoring of nutrients in aquatic systems. Pure Appl Chem. vol. 84, pp. 1973-1982, 2012.
D. T. E. Hunt, A. L. Wilson. The chemical analysis of water. 2nd ed. Oxford: The Alden Press, 1986, pp. 92 ff.
J. Ruzicka, G. D. Marshall. Sequential injection - a new concept for chemical sensors, process analysis and laboratory assays. Anal Chim Acta. vol. 237, pp. 329-343, 1990.
N. Dantan. Fließinjektionsanalyse und sequentielle Injektionsanalyse für Prozessmonitoringaufgaben - Einsatzmöglichkeiten in der pharmazeutischen Industrie. Dissertation, Humboldt-Universität zu Berlin, Berlin, Germany, 2002.
K. N. Andrew, N. J. Blundell, D. Price, P. J. Worsfold. Flow-injection techniques for water monitoring. Anal Chem. vol. 66, pp. 916 A-922 A, 1994.
C. E. Lenehan, N. W. Barnett, S. W. Lewis. Sequential injection analysis. Analyst. vol. 127, pp. 997-1020, 2002.
H. Xu, C. F. Duan, Z. F. Zhang, J. Y. Chen, C. Z. Lai, M. Lian, L. J. Liu, H. Cui. Flow injection determination of p-aminophenol at trace level using inhibited luminol-dimethylsulfoxide-NaOH-EDTA chemiluminescence. Water Res. vol. 39, pp. 396-402, 2005.
K. Agrawal, G. Agnihotri, K. Shrivas, G. L. Mundhara, K. S. Patel, P. Hoffmann. Determination of cationic surfactants in environmental samples by flow injection analysis. Microchim Acta. vol. 147, pp. 273-278, 2004.
P. Mulchandani, W. Chen, A. Mulchandani. Flow injection amperometric enzyme biosensor for direct determination of organophosphate nerve agents. Environ Sci Technol. vol. 35, pp. 2562-2565, 2001.
K. Koshy, M. Mataki. Photochemical oxidation and flow injection conductivity determination of dissolved organic carbon in estuarine and coastal waters. Lab Robotics Automat. vol. 12, pp. 157-163, 2000.
DIN 38407-22:2001-10 - Gemeinsam erfassbare Stoffgruppen (Gruppe F) Teil 22: Bestimmung von Glyphosat und Aminomethylphosphonsäure (AMPA) in Wasser durch Hochleistungs-Flüssigkeitschromatographie (HPLC). Deutsches Institut für Normung e. V.: Berlin, Germany, 2001.
Method 547 - Determination of glyphosate in drinking water by direct-aqueous-injection HPLC, post-column derivatization, and fluorescence detection. U.S. Environmental Protection Agency: Cincinnati, OH, USA, 1990.
B. Jahnke, C. Frank, J. F. Fernández, B. Niemeyer. A sequential injection analysis method for the determination of glyphosate and aminomethylphosphonic acid in water samples. Am Chem Sci J. vol. 5, pp. 163-173, 2015.
S. D. Colombo, J. C. Masini. Developing a fluorimetric sequential injection methodology to study adsorption/desorption of glyphosate on soil and sediment samples. Microchem J. vol. 98, pp. 260-266, 2011.
S. D. Colombo, J. C. Masini. A sequential-injection reversed-phase chromatography method for fluorimetric determination of glyphosate and aminomethylphosphonic acid. Anal Methods-UK. vol. 6, pp. 490-496, 2014.
C. Frank, F. Schroeder. Using sequential injection analysis to improve system and data reliability of online methods: Determination of ammonium and phosphate in coastal waters. J Autom Method Manag. vol. 2007, pp. 1-6, 2007.
C. Frank, F. Schroeder, R. Ebinghaus, W. Ruck. A fast sequential injection analysis system for the simultaneous determination of ammonia and phosphate. Microchim Acta. vol. 154, pp. 31-38, 2006.
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