Study on Biodegradation of Mechanic Workshop Polluted Soil Amended with Lime Fertilizer
International Journal of Environmental Monitoring and Analysis
Volume 4, Issue 1, February 2016, Pages: 21-26
Received: Nov. 7, 2015; Accepted: Nov. 19, 2015; Published: Feb. 1, 2016
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Stephen Emmanuel, Department of Microbiology, Kogi State University, Anyigba-Kogi State, Nigeria
Okwute Loretta Ojonoma, Department of Microbiology, University of Abuja, Gwagwalada- Abuja, Nigeria
Idoko Peter Arome, Department of Microbiology, Kogi State University, Anyigba-Kogi State, Nigeria
Makolo Daniel, School of Preliminary Studies, Kogi State Polytechnic, Kogi State, Nigeria
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Biodegradation of mechanic workshop polluted soil amended with 2.4kg lime (Confluence fertilizer) was studied for a period of eight weeks (56 days). This was done by physicochemical analysis of the soil samples and microbiological analysis by spread plate inoculation on Nutrient agar (NA). The results revealed higher bacteria population in amended soil (AS) compared to oil free soil (OFS) and polluted soil (PS). The range of bacterial counts was Oil free soil (1.6×104 – 6.8 ×104 cfu/g), polluted soil (2.2×104 – 9.8×104 cfu/g) and Amended soil (1.62×104 – 1.12×105 cfu/g). The fungi counts range were Oil free soil (2.5×103 – 3.0×104 cfu/g), polluted soil (2.2×103 – 4.0×104 cfu/g) and Amended soil (2.1×103 – 2.7×103/sup> cfu/g). Eleven (11) organisms were isolated in the course of this study. The bacteria isolated were Pseudomonas putida, Bacillus subtilis, Micrococcus luteus, Proteus mirabilis and Staphylococcus aureus while the fungi isolated include Mucor mucedo, Candida tropicalis, Aspergillus niger, Aspergillus flavus, Aspergillus fumigatus and Rhizopus nigricans. The moisture content (MC), organic carbon, organic matter, pH and nitrate were higher in amended soil compared to unpolluted soil and polluted soil. The result of the study shows that lime (confluence fertilizer) can be used to enhanced biodegradation of oil polluted soil.
Lime, Polluted Soil, Biodegradation, Inoculation
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Stephen Emmanuel, Okwute Loretta Ojonoma, Idoko Peter Arome, Makolo Daniel, Study on Biodegradation of Mechanic Workshop Polluted Soil Amended with Lime Fertilizer, International Journal of Environmental Monitoring and Analysis. Vol. 4, No. 1, 2016, pp. 21-26. doi: 10.11648/j.ijema.20160401.14
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