Cancer Research Journal

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Evaluation of the Performance of PCR and Hybrid Capture 2 in the Detection of Human Papillomavirus Associated with Cervical Cancer in Algeria

Received: 28 May 2018    Accepted: 02 July 2018    Published: 27 July 2018
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Abstract

To evaluate and compare the performance of PCR (polymerase chain reaction) and second-generation capture hybridization (HC2) in the detection of high-risk Human Papillomavirus (HPV-HR) and to estimate their prevalence among normal cervical, dysplastic and cervical cancer in the Algerian population. 264 women were subjected to cyto-histological and colposcopic diagnostics and to the HPV test by PCR and Hybrid capture 2. The search for HPV DNA by conventional PCR was carried out with MY09 / 11 consensus primers and the typing of HPV with the hybridization technique in solution, Digene Sharp Signal for PCR. Hybrid Capture® 2 was performed with the digene HPV HC2 DNA Test kit. The PCR and HC2 performance tests included sensitivity, specificity, positive and negative predictive values, Youden's index, and Yule's Q coefficient. HPV-HR research has shown that these viruses are associated with 100% high-grade lesions, 88.2% by PCR and 83.3% by HC2 (p = 0.8) with invasive cancer. The PCR and HC2 performance tests were in favor of HCV HPV testing which was more sensitive, more specific, more predictive and more effective. The results on the prevalence of HPV-HR associated with cervical lesions are comparable to those in the literature. In comparison with PCR, the capture hybridization technique 2 is recommended for HPV diagnosis because of its better performance and simplicity.

DOI 10.11648/j.crj.20180603.13
Published in Cancer Research Journal (Volume 6, Issue 3, September 2018)
Page(s) 84-91
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Cervical Cancer, Precancerous Lesions, Prevalence, HPV Testing, PCR, Capture Hybridization 2

References
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Author Information
  • Biology Department, Faculty of Science of Nature and Life, University of Oran 1 Ahmed Ben Bella, Oran, Algeria

  • Biology Department, Faculty of Science of Nature and Life, University of Oran 1 Ahmed Ben Bella, Oran, Algeria

  • Institut Pasteur of Algeria, Algiers, Algeria

  • Department of Epidemiology and Preventive Medicine, EHU of Oran, Oran, Algeria

  • Department of Epidemiology and Preventive Medicine, EHU of Oran, Oran, Algeria

  • Gynecology-Obstetrics Department. EPH of Zeralda, Algiers Algeria

  • Pathology Department, Mustapha Pasha University Hospital, Algiers, Algeria

  • Pathology Department, Beni Messous University Hospital, Algiers, Algeria

  • Biology Department, Faculty of Science of Nature and Life, University of Oran 1 Ahmed Ben Bella, Oran, Algeria

Cite This Article
  • APA Style

    Melhag Mohamed, Kerroucha Rabah, Melouli Hamid, Midoun Nori, Zemour Lakhdar, et al. (2018). Evaluation of the Performance of PCR and Hybrid Capture 2 in the Detection of Human Papillomavirus Associated with Cervical Cancer in Algeria. Cancer Research Journal, 6(3), 84-91. https://doi.org/10.11648/j.crj.20180603.13

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    ACS Style

    Melhag Mohamed; Kerroucha Rabah; Melouli Hamid; Midoun Nori; Zemour Lakhdar, et al. Evaluation of the Performance of PCR and Hybrid Capture 2 in the Detection of Human Papillomavirus Associated with Cervical Cancer in Algeria. Cancer Res. J. 2018, 6(3), 84-91. doi: 10.11648/j.crj.20180603.13

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    AMA Style

    Melhag Mohamed, Kerroucha Rabah, Melouli Hamid, Midoun Nori, Zemour Lakhdar, et al. Evaluation of the Performance of PCR and Hybrid Capture 2 in the Detection of Human Papillomavirus Associated with Cervical Cancer in Algeria. Cancer Res J. 2018;6(3):84-91. doi: 10.11648/j.crj.20180603.13

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  • @article{10.11648/j.crj.20180603.13,
      author = {Melhag Mohamed and Kerroucha Rabah and Melouli Hamid and Midoun Nori and Zemour Lakhdar and Ayyach Ghassan and Asselah Fatima and Bendissari Kheira and EL Kebir Fatima Zohra},
      title = {Evaluation of the Performance of PCR and Hybrid Capture 2 in the Detection of Human Papillomavirus Associated with Cervical Cancer in Algeria},
      journal = {Cancer Research Journal},
      volume = {6},
      number = {3},
      pages = {84-91},
      doi = {10.11648/j.crj.20180603.13},
      url = {https://doi.org/10.11648/j.crj.20180603.13},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.crj.20180603.13},
      abstract = {To evaluate and compare the performance of PCR (polymerase chain reaction) and second-generation capture hybridization (HC2) in the detection of high-risk Human Papillomavirus (HPV-HR) and to estimate their prevalence among normal cervical, dysplastic and cervical cancer in the Algerian population. 264 women were subjected to cyto-histological and colposcopic diagnostics and to the HPV test by PCR and Hybrid capture 2. The search for HPV DNA by conventional PCR was carried out with MY09 / 11 consensus primers and the typing of HPV with the hybridization technique in solution, Digene Sharp Signal for PCR. Hybrid Capture® 2 was performed with the digene HPV HC2 DNA Test kit. The PCR and HC2 performance tests included sensitivity, specificity, positive and negative predictive values, Youden's index, and Yule's Q coefficient. HPV-HR research has shown that these viruses are associated with 100% high-grade lesions, 88.2% by PCR and 83.3% by HC2 (p = 0.8) with invasive cancer. The PCR and HC2 performance tests were in favor of HCV HPV testing which was more sensitive, more specific, more predictive and more effective. The results on the prevalence of HPV-HR associated with cervical lesions are comparable to those in the literature. In comparison with PCR, the capture hybridization technique 2 is recommended for HPV diagnosis because of its better performance and simplicity.},
     year = {2018}
    }
    

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    T1  - Evaluation of the Performance of PCR and Hybrid Capture 2 in the Detection of Human Papillomavirus Associated with Cervical Cancer in Algeria
    AU  - Melhag Mohamed
    AU  - Kerroucha Rabah
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    AU  - Asselah Fatima
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    AB  - To evaluate and compare the performance of PCR (polymerase chain reaction) and second-generation capture hybridization (HC2) in the detection of high-risk Human Papillomavirus (HPV-HR) and to estimate their prevalence among normal cervical, dysplastic and cervical cancer in the Algerian population. 264 women were subjected to cyto-histological and colposcopic diagnostics and to the HPV test by PCR and Hybrid capture 2. The search for HPV DNA by conventional PCR was carried out with MY09 / 11 consensus primers and the typing of HPV with the hybridization technique in solution, Digene Sharp Signal for PCR. Hybrid Capture® 2 was performed with the digene HPV HC2 DNA Test kit. The PCR and HC2 performance tests included sensitivity, specificity, positive and negative predictive values, Youden's index, and Yule's Q coefficient. HPV-HR research has shown that these viruses are associated with 100% high-grade lesions, 88.2% by PCR and 83.3% by HC2 (p = 0.8) with invasive cancer. The PCR and HC2 performance tests were in favor of HCV HPV testing which was more sensitive, more specific, more predictive and more effective. The results on the prevalence of HPV-HR associated with cervical lesions are comparable to those in the literature. In comparison with PCR, the capture hybridization technique 2 is recommended for HPV diagnosis because of its better performance and simplicity.
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