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Analysis of Circulating Immune Complexes and Detection of Microbial Antigenic Components in Breast Tumours in Anambra State Nigeria

Received: 13 December 2016     Accepted: 27 December 2016     Published: 25 January 2017
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Abstract

Quantitative measurement of circulating immune complexes in cancer patients is a widely accepted measure linking immune complexes as useful for treatment monitoring. However, the components of these immune complexes are generally understudied. By the analysis of immune complexes, this work elicited some microbial agents that could be associated with breast tumours. Total of 99 female subjects were randomly recruited for this study, of which 24 had benign tumour, 25 had malignant tumour and 50 were tumour free controls subjects. The mean age of the female subjects with benign breast tumour was 37.9±10.1 years; malignant 49.6±10.8 and control subjects 35.9±9.0, and they were confirmed free from chemotherapy, autoimmune diseases and HIV infection. Immune complexes were precipitated and dissociated. Characterization of the antigens/antibodies was done using Enzyme linked immunoassay technique. Microbial antigenic components were detected in 21 benign tumour subjects, 20 malignant tumour subjects and 11 control subjects. Homogeneity and heterogeneity patterns of microbial antigenic distribution were seen in different groups. Heterogeneity pattern was prevalent in subjects with tumours. Detection of HCV was significantly high in subjects with benign and malignant tumours, while presence of Plasmodium falciparum, Helicobacter pylori and Salmonella typhi was also prominent. HBV and treponema pallidum were implicated. Heterogeneity pattern was prevalent in late stages of breast cancer while Plasmodium falciparum, HCV and Salmonella typhi were most prominent. The burden of microbial infection is prevalent in breast tumour subjects. Presence of the microbial antigens would represent the burden of the microbial infection in tumour development and the consequences of the persistent circulation of these microbial proteins were discussed.

Published in Cancer Research Journal (Volume 5, Issue 1)
DOI 10.11648/j.crj.20170501.11
Page(s) 1-8
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2017. Published by Science Publishing Group

Keywords

Circulating Immune Complexes, Breast Tumourigenesis, Microbial Agents, Antigenic Components

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Cite This Article
  • APA Style

    Michael Chukwudi Ezeani, Charles Chinedum Onyenekwe, Samual Chukwuemeka Meludu, Gabriel Udeze Chianakwana, Daniel Chukwuemeka Anyiam, et al. (2017). Analysis of Circulating Immune Complexes and Detection of Microbial Antigenic Components in Breast Tumours in Anambra State Nigeria. Cancer Research Journal, 5(1), 1-8. https://doi.org/10.11648/j.crj.20170501.11

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    ACS Style

    Michael Chukwudi Ezeani; Charles Chinedum Onyenekwe; Samual Chukwuemeka Meludu; Gabriel Udeze Chianakwana; Daniel Chukwuemeka Anyiam, et al. Analysis of Circulating Immune Complexes and Detection of Microbial Antigenic Components in Breast Tumours in Anambra State Nigeria. Cancer Res. J. 2017, 5(1), 1-8. doi: 10.11648/j.crj.20170501.11

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    AMA Style

    Michael Chukwudi Ezeani, Charles Chinedum Onyenekwe, Samual Chukwuemeka Meludu, Gabriel Udeze Chianakwana, Daniel Chukwuemeka Anyiam, et al. Analysis of Circulating Immune Complexes and Detection of Microbial Antigenic Components in Breast Tumours in Anambra State Nigeria. Cancer Res J. 2017;5(1):1-8. doi: 10.11648/j.crj.20170501.11

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  • @article{10.11648/j.crj.20170501.11,
      author = {Michael Chukwudi Ezeani and Charles Chinedum Onyenekwe and Samual Chukwuemeka Meludu and Gabriel Udeze Chianakwana and Daniel Chukwuemeka Anyiam and Comfort Nne Akujobi and Chiemelu Dickson Emegoakor and Martin Ositadimma Ifeanyichukwu and Ujuamala Uloma Ezeani and Nnadozi Okwudili Josiah and Obi Ejeatuluchkwu and Jonathan Madukwe},
      title = {Analysis of Circulating Immune Complexes and Detection of Microbial Antigenic Components in Breast Tumours in Anambra State Nigeria},
      journal = {Cancer Research Journal},
      volume = {5},
      number = {1},
      pages = {1-8},
      doi = {10.11648/j.crj.20170501.11},
      url = {https://doi.org/10.11648/j.crj.20170501.11},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.crj.20170501.11},
      abstract = {Quantitative measurement of circulating immune complexes in cancer patients is a widely accepted measure linking immune complexes as useful for treatment monitoring. However, the components of these immune complexes are generally understudied. By the analysis of immune complexes, this work elicited some microbial agents that could be associated with breast tumours. Total of 99 female subjects were randomly recruited for this study, of which 24 had benign tumour, 25 had malignant tumour and 50 were tumour free controls subjects. The mean age of the female subjects with benign breast tumour was 37.9±10.1 years; malignant 49.6±10.8 and control subjects 35.9±9.0, and they were confirmed free from chemotherapy, autoimmune diseases and HIV infection. Immune complexes were precipitated and dissociated. Characterization of the antigens/antibodies was done using Enzyme linked immunoassay technique. Microbial antigenic components were detected in 21 benign tumour subjects, 20 malignant tumour subjects and 11 control subjects. Homogeneity and heterogeneity patterns of microbial antigenic distribution were seen in different groups. Heterogeneity pattern was prevalent in subjects with tumours. Detection of HCV was significantly high in subjects with benign and malignant tumours, while presence of Plasmodium falciparum, Helicobacter pylori and Salmonella typhi was also prominent. HBV and treponema pallidum were implicated. Heterogeneity pattern was prevalent in late stages of breast cancer while Plasmodium falciparum, HCV and Salmonella typhi were most prominent. The burden of microbial infection is prevalent in breast tumour subjects. Presence of the microbial antigens would represent the burden of the microbial infection in tumour development and the consequences of the persistent circulation of these microbial proteins were discussed.},
     year = {2017}
    }
    

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    T1  - Analysis of Circulating Immune Complexes and Detection of Microbial Antigenic Components in Breast Tumours in Anambra State Nigeria
    AU  - Michael Chukwudi Ezeani
    AU  - Charles Chinedum Onyenekwe
    AU  - Samual Chukwuemeka Meludu
    AU  - Gabriel Udeze Chianakwana
    AU  - Daniel Chukwuemeka Anyiam
    AU  - Comfort Nne Akujobi
    AU  - Chiemelu Dickson Emegoakor
    AU  - Martin Ositadimma Ifeanyichukwu
    AU  - Ujuamala Uloma Ezeani
    AU  - Nnadozi Okwudili Josiah
    AU  - Obi Ejeatuluchkwu
    AU  - Jonathan Madukwe
    Y1  - 2017/01/25
    PY  - 2017
    N1  - https://doi.org/10.11648/j.crj.20170501.11
    DO  - 10.11648/j.crj.20170501.11
    T2  - Cancer Research Journal
    JF  - Cancer Research Journal
    JO  - Cancer Research Journal
    SP  - 1
    EP  - 8
    PB  - Science Publishing Group
    SN  - 2330-8214
    UR  - https://doi.org/10.11648/j.crj.20170501.11
    AB  - Quantitative measurement of circulating immune complexes in cancer patients is a widely accepted measure linking immune complexes as useful for treatment monitoring. However, the components of these immune complexes are generally understudied. By the analysis of immune complexes, this work elicited some microbial agents that could be associated with breast tumours. Total of 99 female subjects were randomly recruited for this study, of which 24 had benign tumour, 25 had malignant tumour and 50 were tumour free controls subjects. The mean age of the female subjects with benign breast tumour was 37.9±10.1 years; malignant 49.6±10.8 and control subjects 35.9±9.0, and they were confirmed free from chemotherapy, autoimmune diseases and HIV infection. Immune complexes were precipitated and dissociated. Characterization of the antigens/antibodies was done using Enzyme linked immunoassay technique. Microbial antigenic components were detected in 21 benign tumour subjects, 20 malignant tumour subjects and 11 control subjects. Homogeneity and heterogeneity patterns of microbial antigenic distribution were seen in different groups. Heterogeneity pattern was prevalent in subjects with tumours. Detection of HCV was significantly high in subjects with benign and malignant tumours, while presence of Plasmodium falciparum, Helicobacter pylori and Salmonella typhi was also prominent. HBV and treponema pallidum were implicated. Heterogeneity pattern was prevalent in late stages of breast cancer while Plasmodium falciparum, HCV and Salmonella typhi were most prominent. The burden of microbial infection is prevalent in breast tumour subjects. Presence of the microbial antigens would represent the burden of the microbial infection in tumour development and the consequences of the persistent circulation of these microbial proteins were discussed.
    VL  - 5
    IS  - 1
    ER  - 

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Author Information
  • Department of Immunology, Faculty of Medicine, Nnamdi Azikiwe University, Awka, Nigeria

  • Department of Medical Laboratory Science, Faculty of Health Science and Technology, Nnamdi Azikiwe University, Awka, Nigeria

  • Department of Human Biochemistry, Faculty of Basic Medical Sciences, Nnamdi Azikiwe University, Awka, Nigeria

  • Department of Surgery, Faculty of Medicine, Nnamdi Azikiwe University, Awka, Nigeria

  • Histopathology Department, Faculty of Medicine, Nnamdi Azikiwe University, Awka, Nigeria

  • Department of Medical Microbiology/Parasitology, Faculty of Medicine, Nnamdi Azikiwe University, Awka, Nigeria

  • Department of Surgery, Faculty of Medicine, Nnamdi Azikiwe University, Awka, Nigeria

  • Department of Medical Laboratory Science, Faculty of Health Science and Technology, Nnamdi Azikiwe University, Awka, Nigeria

  • Medical Centre, Pharmaceutical Unit, Nnamdi Azikiwe University, Nnewi Campus, Nigeria

  • Department of Chemical Pathology, Medical Laboratory Section, Nnamdi Azikiwe University Teaching Hospital, Nnewi, Nigeria

  • Pharmacology Department, Faculty of Medicine, Nnamdi Azikiwe University Awka, Nigeria

  • Histopathology Department, National Hospital, Abuja, Nigeria

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