Background: Breast cancer is the second most common cancer among women after cervical cancer. As Cancer development and progression is dictated by chain of alterations in genes. Over the past few years, the Kashmir valley has witnessed a tremendous increase in the incidence of breast cancer in its unexplored ethnic population. The aim of present study was to find out the role of Promoter Hypermethylation of BRCA1 gene in Breast cancer patients. Material Methods: The DNA was extracted from all the samples and was modified using bisulphite modification kit. Methylation-specific polymerase chain reaction was used for the analysis of the promoter hypermethylation status of BRCA1 gene. Results: The epigenetic analysis revealed that unlike other high risk regions, Kashmiri population has a different promoterhypermethylation profile of BRCA1 gene as 68% of the cases showed BRCA1 promoter hypermethylation in comparison to 20% of the normal cases, the association of promoter hypermethylation with breast cancer and normal cases was found to be significant (P=0.0006). The frequency of BRCA1 promoter hypermethylation was found to be certainly higher in Stage III/IV (75%) compared to Stage I/ II (62%) but the difference was not statistically significant(P =0.0674). The frequency of promoter methylation was foundhigher (77.1%) in age group above 60- years) than ages below 60 years. Conclusion: These results suggest that BRCA1 aberrant promoter hypermethylation in Kashmiri population contributes to the process of carcinogenesis in breast cancer and is reportedly one of the commonest epigenetic changes in the development of breast cancer.
| Published in | Cancer Research Journal (Volume 5, Issue 2) |
| DOI | 10.11648/j.crj.20170502.11 |
| Page(s) | 9-13 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2024. Published by Science Publishing Group |
Breast Cancer, BRCA1, Hypermethylation, Bisulphite Treatment
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APA Style
Tehseen Hassan, Showkat Ahmad Bhat, Sabhiya Majid, Manzoor R. Mir, Purnima Shrivastava. (2017). BRCA1 Promoter Hypermethylationas an Early Diagnostic Tool for Breast Cancer. Cancer Research Journal, 5(2), 9-13. https://doi.org/10.11648/j.crj.20170502.11
ACS Style
Tehseen Hassan; Showkat Ahmad Bhat; Sabhiya Majid; Manzoor R. Mir; Purnima Shrivastava. BRCA1 Promoter Hypermethylationas an Early Diagnostic Tool for Breast Cancer. Cancer Res. J. 2017, 5(2), 9-13. doi: 10.11648/j.crj.20170502.11
AMA Style
Tehseen Hassan, Showkat Ahmad Bhat, Sabhiya Majid, Manzoor R. Mir, Purnima Shrivastava. BRCA1 Promoter Hypermethylationas an Early Diagnostic Tool for Breast Cancer. Cancer Res J. 2017;5(2):9-13. doi: 10.11648/j.crj.20170502.11
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Download@article{10.11648/j.crj.20170502.11,
author = {Tehseen Hassan and Showkat Ahmad Bhat and Sabhiya Majid and Manzoor R. Mir and Purnima Shrivastava},
title = {BRCA1 Promoter Hypermethylationas an Early Diagnostic Tool for Breast Cancer},
journal = {Cancer Research Journal},
volume = {5},
number = {2},
pages = {9-13},
doi = {10.11648/j.crj.20170502.11},
url = {https://doi.org/10.11648/j.crj.20170502.11},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.crj.20170502.11},
abstract = {Background: Breast cancer is the second most common cancer among women after cervical cancer. As Cancer development and progression is dictated by chain of alterations in genes. Over the past few years, the Kashmir valley has witnessed a tremendous increase in the incidence of breast cancer in its unexplored ethnic population. The aim of present study was to find out the role of Promoter Hypermethylation of BRCA1 gene in Breast cancer patients. Material Methods: The DNA was extracted from all the samples and was modified using bisulphite modification kit. Methylation-specific polymerase chain reaction was used for the analysis of the promoter hypermethylation status of BRCA1 gene. Results: The epigenetic analysis revealed that unlike other high risk regions, Kashmiri population has a different promoterhypermethylation profile of BRCA1 gene as 68% of the cases showed BRCA1 promoter hypermethylation in comparison to 20% of the normal cases, the association of promoter hypermethylation with breast cancer and normal cases was found to be significant (P=0.0006). The frequency of BRCA1 promoter hypermethylation was found to be certainly higher in Stage III/IV (75%) compared to Stage I/ II (62%) but the difference was not statistically significant(P =0.0674). The frequency of promoter methylation was foundhigher (77.1%) in age group above 60- years) than ages below 60 years. Conclusion: These results suggest that BRCA1 aberrant promoter hypermethylation in Kashmiri population contributes to the process of carcinogenesis in breast cancer and is reportedly one of the commonest epigenetic changes in the development of breast cancer.},
year = {2017}
}
TY - JOUR T1 - BRCA1 Promoter Hypermethylationas an Early Diagnostic Tool for Breast Cancer AU - Tehseen Hassan AU - Showkat Ahmad Bhat AU - Sabhiya Majid AU - Manzoor R. Mir AU - Purnima Shrivastava Y1 - 2017/06/23 PY - 2017 N1 - https://doi.org/10.11648/j.crj.20170502.11 DO - 10.11648/j.crj.20170502.11 T2 - Cancer Research Journal JF - Cancer Research Journal JO - Cancer Research Journal SP - 9 EP - 13 PB - Science Publishing Group SN - 2330-8214 UR - https://doi.org/10.11648/j.crj.20170502.11 AB - Background: Breast cancer is the second most common cancer among women after cervical cancer. As Cancer development and progression is dictated by chain of alterations in genes. Over the past few years, the Kashmir valley has witnessed a tremendous increase in the incidence of breast cancer in its unexplored ethnic population. The aim of present study was to find out the role of Promoter Hypermethylation of BRCA1 gene in Breast cancer patients. Material Methods: The DNA was extracted from all the samples and was modified using bisulphite modification kit. Methylation-specific polymerase chain reaction was used for the analysis of the promoter hypermethylation status of BRCA1 gene. Results: The epigenetic analysis revealed that unlike other high risk regions, Kashmiri population has a different promoterhypermethylation profile of BRCA1 gene as 68% of the cases showed BRCA1 promoter hypermethylation in comparison to 20% of the normal cases, the association of promoter hypermethylation with breast cancer and normal cases was found to be significant (P=0.0006). The frequency of BRCA1 promoter hypermethylation was found to be certainly higher in Stage III/IV (75%) compared to Stage I/ II (62%) but the difference was not statistically significant(P =0.0674). The frequency of promoter methylation was foundhigher (77.1%) in age group above 60- years) than ages below 60 years. Conclusion: These results suggest that BRCA1 aberrant promoter hypermethylation in Kashmiri population contributes to the process of carcinogenesis in breast cancer and is reportedly one of the commonest epigenetic changes in the development of breast cancer. VL - 5 IS - 2 ER -
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