Progesterone Induces NFκB DNA Binding Activity through a PI3K/Akt-Dependent Pathway in MCF-7 Breast Cancer Cells
Cancer Research Journal
Volume 2, Issue 4, July 2014, Pages: 63-69
Received: Jun. 13, 2014; Accepted: Jun. 28, 2014; Published: Jul. 10, 2014
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Authors
Fernando Candanedo-Gonzalez, Departamento de Biologia Celular. Cinvestav-IPN. Av IPN # 2508. San Pedro Zacatenco. Mexico, DF. 07360. Mexico
Octavio Galindo-Hernandez, Departamento de Biologia Celular. Cinvestav-IPN. Av IPN # 2508. San Pedro Zacatenco. Mexico, DF. 07360. Mexico
Nathalia Serna-Marquez, Departamento de Biologia Celular. Cinvestav-IPN. Av IPN # 2508. San Pedro Zacatenco. Mexico, DF. 07360. Mexico
Roberto Espinosa-Neira, Departamento de Biologia Celular. Cinvestav-IPN. Av IPN # 2508. San Pedro Zacatenco. Mexico, DF. 07360. Mexico
Adriana Soto-Guzman, Departamento de Biologia Celular. Cinvestav-IPN. Av IPN # 2508. San Pedro Zacatenco. Mexico, DF. 07360. Mexico
Pedro Cortes-Reynosa, Departamento de Biologia Celular. Cinvestav-IPN. Av IPN # 2508. San Pedro Zacatenco. Mexico, DF. 07360. Mexico
Eduardo Perez Salazar, Departamento de Biologia Celular. Cinvestav-IPN. Av IPN # 2508. San Pedro Zacatenco. Mexico, DF. 07360. Mexico
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Abstract
Progesterone (PG) is a steroid hormone that regulates normal reproductive functions including uterine and mammary gland development. The PG receptor belongs to the nuclear receptor superfamily of ligand dependent transcription factors that mediates gene expression, however, it also promotes cell signaling pathways through a non-genomic pathway including the activation of PI3K/Akt and Src/ERK1/2 pathways. However the role of PG in NFκB-DNA complex formation remains to be studied. We demonstrate here that PG induces Akt2 activation and an increase of NFκB DNA binding activity in MCF-7 breast cancer cells. Akt2 activation requires PI3K activity, whereas increase of NFκB DNA binding activity requires PI3K, mTOR, Akt, Src, and G-proteins activity, as well as the integrity of cytoskeleton. In summary, our findings demonstrate that PG induces an increase of NFκB DNA binding activity in MCF-7 cells.
Keywords
Breast Cancer, Progesterone, PI3K/Akt, NFκB, MCF-7
To cite this article
Fernando Candanedo-Gonzalez, Octavio Galindo-Hernandez, Nathalia Serna-Marquez, Roberto Espinosa-Neira, Adriana Soto-Guzman, Pedro Cortes-Reynosa, Eduardo Perez Salazar, Progesterone Induces NFκB DNA Binding Activity through a PI3K/Akt-Dependent Pathway in MCF-7 Breast Cancer Cells, Cancer Research Journal. Vol. 2, No. 4, 2014, pp. 63-69. doi: 10.11648/j.crj.20140204.11
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