Empirical Studies about Inhibiting Proliferation of Zedoary Turmeric oil, Cinobufacini and Solanine on Gastric Carcinoma Cells
Cancer Research Journal
Volume 2, Issue 3, May 2014, Pages: 42-46
Received: Apr. 17, 2014;
Accepted: May 23, 2014;
Published: Jun. 10, 2014
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Xuejun Zhan, Department of Detection Center, Key Laboratory of Biological Medical of Jiangxi, Jiangxi Academy of Medical Science, Jiangxi, Nanchang, China
Daze Xie, Department of Detection Center, Key Laboratory of Biological Medical of Jiangxi, Jiangxi Academy of Medical Science, Jiangxi, Nanchang, China
Yingyin Hu, Cancer Research Laboratory, Key Laboratory of Biological Medical of Jiangxi, Jiangxi Academy of Medical Science, Jiangxi, Nanchang, China
Ge Dai, Department of Nuclear Medicine, Jiangxi Academy of Medical Science, Jiangxi, Nanchang, China
Yanping Xu, Department of Detection Center, Key Laboratory of Biological Medical of Jiangxi, Jiangxi Academy of Medical Science, Jiangxi, Nanchang, China
Purpose: To investigate the anti-proliferative effects of anti-cancer Chinese medicinal materials such as zedoary turmeric oil, cinobufacini and solanine on gastric carcinoma cell line NKM45. Methods: Bioluminescence assay (BLA) was applied to determine the ATP concentration when NKM45 was exposed to the three Chinese medicinal materials. The influence of zedoary turmeric oil on the growth curve of NKM45 cells was also analyzed. And the concentration of ATP was compared with NKM45 cells that were exposed to 5-Fluorouracil(5-Fu). Results: The concentration of ATP was significantly decreased when the NKM45 cells were exposed to the three Chinese medicinal materials and 5-Fu (P<0.05~P<0.01). There was no significant difference in the ATP concentration between MKM45 cells that were treated with the three Chinese medicinal materials and those exposed to 5-Fu (P>0.05). The zedoary turmeric oil significantly inhibited the survival rate of NKM45 cells. Conclusions: Zedoary turmeric oil, cinobufacin, and solanine can inhibit the proliferative activity of NKM45 cells. They have exploitation potential with broad market prospect.
Empirical Studies about Inhibiting Proliferation of Zedoary Turmeric oil, Cinobufacini and Solanine on Gastric Carcinoma Cells, Cancer Research Journal.
Vol. 2, No. 3,
2014, pp. 42-46.
Zhou H, Wang Q, Jiang Zh Sh. Advance in anti-tumor of zedoary turmeric oil. Tumor Journal of World.2003; 2（4）:325-328.
Yang F Q, L i S P, Chen Y, et al Identification and quantitation of eleven sesquiterpenes in three species of Curcuma rhizomes by pressurized liquid extraction and gas chromatography–mass spectrometry. Journal of Pharmaceutical and Biomedical Analysis. 2006;39（3-4）: 662-668. DOI:10.1016/j.jpbc.2005.05.001
You J, Cui F D, H an X, et al. Study of the preparation of sus-tained-release microspheres containing zedoary turmeric oil by the emulsion-solvent-diffusion method and evaluation of the self-emulsification and bioavailability of the oil. Colloids and Surfaces B: Biointerfaces.2006;48（1）：35-41. DOI:10.1016/j.colsurfb.2005.12.011
Zhang BL, Wu H. Study progress on anti-tumor and its mechanism of cinobufagini. Chinese and Foreign Medical Research.2012; 10（14）:152-154
Gui XL. Mechanism research advance in anti-tumor of solanine. Chinese Journal of Information on Traditional Chinese Medi-cine.2009;16(suppl):80-82
Zhao X Q, Zeng X F. Clinical study of Longkui tablet on primary carcinoma of the liver. Liaoning Traditional Chinese Medical.2002;29(11)；671-672
JasonM Walvaven, JohnOTrent, DavidW. Hein. Structure- function analyses of single nucleotide polymorphism in humanN-acetyltransferase1. Drug Metabolism Reviews, 2008;40(1):169-184. DOI:10.1080/03602530701852917
Liu WW, Liu YQ, Dai XJ. Study advance on anti-cancer effects of Solanum NigrumL..Journal of Chinese Medicinal Materials. 2009;32(3): 462-465
Qi F, Li A, Inagaki Y, etal. Antitumor activity of extracts and compounds from the skin of the toad Bufobufo gargarizans Cantor. International Immunopharmacogogy.2011;11(3):342-349. DOI:10.1016/j.intimp.2010.12.007
Tang Y, Li XH. Inhibitory effects of Zedoary Rhizome abstraction hepatic cell line HepG-2 and the mechanisms. Pharmacological Bulletin. 2007; 23(6):790-794
.Kangas L, Gronroos M, Nieminen AL. Bioluminescence of cellular ATP: a new method for evaluating cytotoxic agents in vitro.Medical Biology.1984;62:338- 343. PMID: 6543460
Kurbacher CM, Bruckner HW, Andreotti PE, et al. In vitro activity of titanocenedichloride versus cisplatin in four ovarian carcinoma cell lines evaluated by a microtiter plate ATP bioluminescence assay. Anticancer Drugs. 1995;6(5): 697-704. PM-ID:8845481
Crouch SP, Kozlowski R, Slater KJ, et al. The use of ATP bioluminescence as a measure of cell proliferation and cytotoxicity.Journal of ImmunologicalMethods. 1993;160 (1): 81-88. DOI:10.1016/0022-1769(93)90011-U
Chen DC. Work book of Chinese Medicine Chemical Contrasts. Chinese Medicine Scientific and Technical Press, Beijing 2000;.6-7
Sevin BU, Peng ZL, Pervas JP, et al. Application on ATP-bioluminescence assay in human tumor chemosensitivity test-ing.GynecologicOncology.1988;31(1):191-204.DOI:1016/0090-8258(88)9093-4
Xu RC, Chen XY, Chen L, et al. Preliminary study on apoptosis of human leukemic cells induced by bufalin. China Journal of Chinese Materia Medica.2001;26:59-61. PMID:12525125
Hou Y, Wu XQ, Wang XH. Research on bioluminescent theory and applying of ATP. China Medical Herald, 2010;7(12):12-18.
Sevin BU, Pervas JP, Averette HE, et al. Chemosensitivity test in ginovarian cancer. Cancer. 1993; 71(4):1613-1620. DOI:10.1002/cncr2820710428
Xu ChG. Clinical efficacy of integration in the treatment of advanced malignant tumor and development of anticancer traditional Chinese Medicine. Medical Innovation of China. 2013;10(18):141-142 DOI:10.3969/j.issn.1674-4985.2013.18.069
Peng BX, He X, Shi JSh, et al. Effects of volatile oil and three main components from curcum a phaeocaulis valeton on liver cancer and endometrial carcinoma cell lines . West China Journal of Pharmaceutical Science.2007; 22(3):312-313
Li GD, Xu F, Shen AJ. Studies progress of zedoary turmeric oil. Chinese Pharmaceutical Journal. 2002; 37(11):.806-809.
Li D, Qu X, Hou K, et al. PI3K/Akt is involved in bufalin-induced apoptosis in gastric cancer cells. AnticancerDrugs.2009;20(1):59-64. DOI:10.1097/ CAD.0b013e32831160fd6
Zuo XD, Cui YA. Clinical studies advance in anti-tumor of cinobufacini. Chinese Clinical Oncology. 2003; 8:232-235.
Son YO, Kim J ,Lim JC, et al. Ripe fruits of Solanum nigrum L. inhibits cell growth and induces apoptosis in MCF-7 cells. Food and Chemical Toxicology.2003;41(10):1421-1428. DOI:10.1016/S0278-6915(03)00161-3
Yen GC, Chen HY, Peng HH. Evaluation of the cytotoxicity, mu-tagenicity and anti-mutagenicity of emerging edible plants.Food and Chemical Toxicology.2001;39,(11):1045-1053. PMID:11527563
Li PW. Practical clinical anti-tumor Chinese medicinal materials. Liaoning Scientific and Technical Press, Shenyang. 2000:22-23
Li Y, Lin AH. The latest research progress of zedoary turmeric oil. China practical Medicine. 2012;7(5): 243-244
Mei QX, Zhang ZhQ, Guan J, et al. Research progress in clinical application of solanum nigrumL. Asia-Pacific Traditional Medicine. 2011;7(11):168-170
Wang TT, Xu GX. Advancement on study of pharmacological action and clinical application of cinobufacini. International Journal of Ophthalmolgy. 2009;9(7):1330-1333