Journal of Food and Nutrition Sciences

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Differential Inhibition of the Rhythm and Amplitude of Acetylcholine-Dependent Contraction in the Murine Jejunum and Ileum In Vitro by Thiamin and Quinine

Received: 05 July 2018    Accepted: 16 July 2018    Published: 22 October 2018
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Abstract

Previously, the effects of several bitter substances have been investigated in the contraction of the murine jejunum and ileum, reporting that these independently suppress the rhythm generation of the interstitial cells of Cajal. Recently, it was reported that thiamin, which binds to a bitter taste receptor, modifies the early phase of the ileum contraction, whereas the physiological effects on the rhythm and amplitude of jejunum and ileum contractions remain unclear. In this study, it was investigated the physiological effects of thiamin and quinine on the in vitro contraction of the murine jejunum and ileum using mice for all experiments. the periodic contraction of the jejunum was observed before the administration of acetylcholine (Ach) and other substances, and the tonic amplitudes induced by the substances. These bitter substances variably affect the Ach-induced rhythmic contraction of the jejunum and ileum in vitro. In addition, quinine hydrochloride (Qui) and thiamin hydrochloride (Thi) variably affect the Ach-induced rhythmic contraction of the jejunum and ileum in vitro. Both Qui and Thi markedly increase the rhythmic contraction in the jejunum. Although Thi does not change the rhythmic contraction in the ileum, it gradually reduces the amplitude in the jejunum. Conversely, Qui gradually reduces the amplitude and almost inhibits the contraction in the jejunum. Furthermore, an antagonist of the adrenalin-beta3 receptor, SR59230A, enhances the Qui-induced inhibition of the contraction in the jejunum.

DOI 10.11648/j.jfns.20180605.11
Published in Journal of Food and Nutrition Sciences (Volume 6, Issue 5, September 2018)
Page(s) 115-122
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Thiamin, Quinine, Small Intestine, Mouse

References
[1] Sanders, K. M., Ward S. M., Koh, S. D. (2014). Interstitial cells: regulators of smooth muscle function. Physiol. Rev. 94:859-907.
[2] Huizinga, J. D., Chen, J. H. (2014). Interstitial cells of Cajal: update on basic and clinical science. Curr. Gastroenterol. Rep. 16:363.
[3] Lies, B., Gil, V., Groneberg, D., Seidler, B., Saur, D., Wischmeyer, E., Jiménez, M., Friebe, A. (2014). Interstitial cells of Cajal mediate nitrergic inhibitory neurotransmission in the murine gastrointestinal tract. Am. J. Physiol. Gastrointest. Liver Physiol. 307:G98-106.
[4] Al-Shboul, O. A. (2013). The importance of interstitial cells of cajal in the gastrointestinal tract. Saudi J. gastroenterol. 19 (1): 3.
[5] Lee, H. T., Hennig, G. W., Fleming, N. W., Keef, K. D., Spencer, N. J., Ward, S. M., Smith, T. K. (2007). The mechanism and spread of pacemaker activity through myenteric interstitial cells of Cajal in human small intestine. Gastroenterol. 132: 1852–1865.
[6] Farrugia, G. (2008). Interstitial cells of Cajal in health and disease. Neurogastroenterol. Motility, 20: 54–63.
[7] Garcia-Lopez, P., Garcia-Marin, V., Martínez-Murillo, R., Freire, M. (2009). Updating old ideas and recent advances regarding the Interstitial Cells of Cajal. Brain Res. Reviews, 61: 154–169.
[8] Huizinga, J. D., Chen, J., Mikkelsen, H. B., Wang, X. Y., Parsons, S., Zhu, Y. (2013). Interstitial cells of Cajal, from structure to function. Front. neurosci. 7:43.
[9] Blair, P. J., Rhee, P. L., Sanders, K. M., Ward, S. M. (2014). The significance of interstitial cells in neurogastroenterology. J. Neurogastroenterol. Motil. 20: 294–317.
[10] Baker, S. A., Drumm, B. T., Skowronek, K. E., Rembetski, B. E., Peri, L. E. Hennig, G. W., Perrino, B. A., Sanders, K. M. (2018). Excitatory Neuronal Responses of Ca2+ Transients in Interstitial Cells of Cajal in the Small Intestine. eNeuro. 5: 0080–18.
[11] Sanders, K. M., Koh, S. D., Ro, S., Ward, S. M. (2012). Regulation of gastrointestinal motility- insights from smooth muscle biology. Nature Reviews: Gastroenterol. Hepatol. 9: 633–645.
[12] Koh, S. D., Ward, S. M., Sanders, K. M. (2012). Ionic conductances regulating the excitability of colonic smooth muscles. Neurogastroenterol. Motil. 24: 705–718.
[13] Hanani, M., Farrugia, G., Komuro, T. (2005). Intercellular coupling of interstitial cells of Cajal in the digestive tract. Int. Rev. Cytol. 242: 249–282.
[14] Seki, K., Komuro, T. (2001). Immunocytochemical demonstration of the gap junction proteins connexin 43 and connexin 45 in the musculature of the rat small intestine. Cell Tissue Res. 306: 417–422.
[15] Miduturu, S., Hoppersta, M. G., Spray, D. C. (2001). Quinine blocks specific gap junction channel subtypes. Proc. Natl. Acad. Sci. USA. 98: 10942–10947.
[16] Gajda, Z., Szupera, Z., Blazsó, G., Szente, M. (2005). Quinine, a blocker of neuronal cx36 channels, suppresses seizure activity in rat neocortex in vivo. Epilepsia. 46: 1581–1591.
[17] Uchiyama, T., Chess-Williams, R. (2004). Muscarinic receptor subtypes of the bladder and gastrointestinal tract. J. Smooth Muscle Res. 40: 237–247.
[18] Stephens, G. J., Mochida, S. (2005). G protein β subunits mediate presynaptic inhibition of transmitter release from rat superior cervical ganglion neurones in culture. J. Physiol 563: 765–776.
[19] Tanaka, Y., Horinouchi, T., Koike, K. (2005). Newinsightsinto β-adrenoceptorsin smooth muscle: distribution of receptor subtypes and molecular mechanisms triggering muscle relaxation. Clin. Exp. Pharmacol. Physiol. 32: 503–514.
[20] Kim, H. J., Han, T., Kim, Y. T., So, I., Kim, B. J. (2017). Magnolia Officinalis Bark Extract Induces Depolarization of Pacemaker Potentials Through M2 and M3 Muscarinic Receptors in Cultured Murine Small Intestine Interstitial Cells of Cajal. Cell. Physiol. Biochem. 43: 1790–1802.
[21] Gim, H., Nam, J. H., Lee, S., Shim, J. H., Kim, H. J., Ha, K. T., Kim, B. J. (2015). Quercetin inhibits pacemaker potentials via nitric oxide/cGMP-dependent activation and TRPM7/ANO1 channels in cultured interstitial cells of Cajal from mouse small intestine. Cell. Physiol. Biochem. 35: 2422–2436.
[22] Jin, N. G., Koh, S. D., Sanders, K. M. (2009). Caffeine inhibits nonselective cationic currents in interstitial cells of Cajal from the murine jejunum. Amer. J. Physiol. Cell Physiol. 297: C971–C978.
[23] Tazzeo, T. Bates, G. Roman, H. N. Lauzon, A. M. Khasnis, M. D. Eto, M. Janssen, L. J. (2012) Caffeine relaxes smooth muscle through actin depolymerization. Amer. J. Physiol. Lung. Cell Mol. Physiol. 303: L334–42.
[24] Yamashita, A., Shimamoto, N., Morita, K., Sugiyama, H., Kimoto, M., Toda, K., Ota M. (2018) Thiamin and Quinine Differently Inhibit the Early Phase of Acetylcholine-Dependent Contraction of Mouse Ileum in vitro. Int. J. Nutr. Food Sci. 7: 94–99.
[25] Wu, M. J., Shin, D. H., Kim, M. Y., Park, C. G., Kim, Y. D., Lee, J., Park, I. K., Choi, S., So, I., Park, J. S., Jun, J. Y. (2015). Functional effects of β3-adrenoceptor on pacemaker activity in interstitial cells of Cajal from the mouse colon. Eur. J. Pharmacol. 754:32–40.
[26] Goyal, R. K., Chaudhury, A. (2010). Mounting evidence against the role of ICC in neurotransmission to smooth muscle in the gut. Am. J. Physiol. Gastrointest. Liver Physiol. 298: G10–3.
[27] Klein, S., Seidler, B., Kettenberger, A., Sibaev, A., Rohn, M., Feil, R., Allescher, H. D., Vanderwinden, J. M., Hofmann, F., Schemann, M., Rad, R., Storr, M. A., Schmid, R. M., Schneider, G., Saur, D. (2013). Interstitial cells of Cajal integrate excitatory and inhibitory neurotransmission with intestinal slow-wave activity. Nat. Commun. 4: 1630.
[28] Hutchinson, D. S., Evans, B. A., Summers R. J. (2001). Beta (1)-Adrenoceptors compensate for beta (3)-adrenoceptors in ileum from beta (3)-adrenoceptor knock-out mice. Br. J. Pharmacol. 132: 433–42.
[29] Jun, J. Y., Choi, S., Yeum, C. H., Chang, I. Y., Park, C. K., Kim, M. Y., Kong, I. D., So, I., Kim, K. W., You H. J. (2004). Noradrenaline inhibits pacemaker currents through stimulation of β1‐adrenoceptors in cultured interstitial cells of Cajal from murine small intestine. Br. J. pharmacol. 141: 670–677.
[30] Lee, M. C., Ha, W., Park, J., Kim, J., Jung, Y., Kim, B. J. (2016). Effects of Lizhong Tang on gastrointestinal motility in mice. World J. gastroenterol. 22: 7778.
[31] Sung, S. K., Kim, S. J., Ahn, T. S., Hong, N. R., Park, H. S., Kwon, Y. K., Kim, B. J. (2015). Effects of Dangkwisoo‑san, a traditional herbal medicine for treating pain and blood stagnation, on the pacemaker activities of cultured interstitial cells of Cajal. Mol. Med. Reports. 12: 6370–6376.
[32] Jalali-Nezhad, A. A., Frajan-Mahhadi, F., Komeili, G., Barkhordari-Ahmadi, F. (2015) The effect of ginger hydroalcholicextract on rat ileal contraction in vitro. Zahedan J. Res. Med. Sci. 15:29-33.
[33] Kim, H. J., Park, S. Y., Kim, D. G., Park, S. H., Lee, H., Hwang, D. Y., Kim, B. J. (2016). Effects of the roots of Liriope Platyphylla Wang Et tang on gastrointestinal motility function. J. Ethnopharmacol., 184: 144–153.
[34] Kim, H. J., Kim, B. J. (2017). Naringenin inhibits pacemaking activity in interstitial cells of Cajal from murine small intestine. Integrative Med. Res, 6:149–155.
[35] Sharma, S., Sheehy, T., Kolonel, L. N. (2013). Ethnic differences in grains consumption and their contribution to intake of B-vitamins: results of the Multiethnic Cohort Study. Nutr. J. 12: 65.
[36] Romanenko, A. V., Gnatenko, V. M., Vladimirova, I. A. (1994). Effect of thiamin on neuromuscular transmission in smooth muscles. Neurophysiol. 26: 370–377.
[37] Bettendorff, L., Wins, P. (2013). Biological functions of thiamin derivatives: Focus on non-coenzyme roles. OA Biochem., 1:10.
[38] Waldenlind, L. (1979). Possible role of thiamin in neuromuscular transmission. Acta Physiol. Scand. 105:1-10.
[39] Manzetti, S., Zhang, J., van der Spoel, D. (2014). Thiamin function, metabolism, uptake, and transport. Biochemistry. 53:821-35.
[40] Brown, G. (2014). Defects of thiamine transport and metabolism. J. Inherit. Metab. Dis. 37:577-85.
Author Information
  • Laboratory of Anatomy, Physiology, and Food Biological Science, Department of Food and Nutrition, Japan Women's University, Tokyo, Japan

  • Laboratory of Anatomy, Physiology, and Food Biological Science, Department of Food and Nutrition, Japan Women's University, Tokyo, Japan

  • Laboratory of Anatomy, Physiology, and Food Biological Science, Department of Food and Nutrition, Japan Women's University, Tokyo, Japan

  • Laboratory of Anatomy, Physiology, and Food Biological Science, Department of Food and Nutrition, Japan Women's University, Tokyo, Japan

  • Laboratory of Anatomy, Physiology, and Food Biological Science, Department of Food and Nutrition, Japan Women's University, Tokyo, Japan

  • Laboratory of Anatomy, Physiology, and Food Biological Science, Department of Food and Nutrition, Japan Women's University, Tokyo, Japan

  • Laboratory of Anatomy, Physiology, and Food Biological Science, Department of Food and Nutrition, Japan Women's University, Tokyo, Japan

  • Integrative Sensory Physiology, Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki, Japan

  • Laboratory of Anatomy, Physiology, and Food Biological Science, Department of Food and Nutrition, Japan Women's University, Tokyo, Japan

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    Atsuko Yamashita, Nana Shimamoto, Kyoko Morita, Hasumi Sugiyama, Shiho Tadakuma, et al. (2018). Differential Inhibition of the Rhythm and Amplitude of Acetylcholine-Dependent Contraction in the Murine Jejunum and Ileum In Vitro by Thiamin and Quinine. Journal of Food and Nutrition Sciences, 6(5), 115-122. https://doi.org/10.11648/j.jfns.20180605.11

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    Atsuko Yamashita; Nana Shimamoto; Kyoko Morita; Hasumi Sugiyama; Shiho Tadakuma, et al. Differential Inhibition of the Rhythm and Amplitude of Acetylcholine-Dependent Contraction in the Murine Jejunum and Ileum In Vitro by Thiamin and Quinine. J. Food Nutr. Sci. 2018, 6(5), 115-122. doi: 10.11648/j.jfns.20180605.11

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    AMA Style

    Atsuko Yamashita, Nana Shimamoto, Kyoko Morita, Hasumi Sugiyama, Shiho Tadakuma, et al. Differential Inhibition of the Rhythm and Amplitude of Acetylcholine-Dependent Contraction in the Murine Jejunum and Ileum In Vitro by Thiamin and Quinine. J Food Nutr Sci. 2018;6(5):115-122. doi: 10.11648/j.jfns.20180605.11

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  • @article{10.11648/j.jfns.20180605.11,
      author = {Atsuko Yamashita and Nana Shimamoto and Kyoko Morita and Hasumi Sugiyama and Shiho Tadakuma and Maki Kato and Mari Kimoto and Kazuo Toda and Masato Ota},
      title = {Differential Inhibition of the Rhythm and Amplitude of Acetylcholine-Dependent Contraction in the Murine Jejunum and Ileum In Vitro by Thiamin and Quinine},
      journal = {Journal of Food and Nutrition Sciences},
      volume = {6},
      number = {5},
      pages = {115-122},
      doi = {10.11648/j.jfns.20180605.11},
      url = {https://doi.org/10.11648/j.jfns.20180605.11},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.jfns.20180605.11},
      abstract = {Previously, the effects of several bitter substances have been investigated in the contraction of the murine jejunum and ileum, reporting that these independently suppress the rhythm generation of the interstitial cells of Cajal. Recently, it was reported that thiamin, which binds to a bitter taste receptor, modifies the early phase of the ileum contraction, whereas the physiological effects on the rhythm and amplitude of jejunum and ileum contractions remain unclear. In this study, it was investigated the physiological effects of thiamin and quinine on the in vitro contraction of the murine jejunum and ileum using mice for all experiments. the periodic contraction of the jejunum was observed before the administration of acetylcholine (Ach) and other substances, and the tonic amplitudes induced by the substances. These bitter substances variably affect the Ach-induced rhythmic contraction of the jejunum and ileum in vitro. In addition, quinine hydrochloride (Qui) and thiamin hydrochloride (Thi) variably affect the Ach-induced rhythmic contraction of the jejunum and ileum in vitro. Both Qui and Thi markedly increase the rhythmic contraction in the jejunum. Although Thi does not change the rhythmic contraction in the ileum, it gradually reduces the amplitude in the jejunum. Conversely, Qui gradually reduces the amplitude and almost inhibits the contraction in the jejunum. Furthermore, an antagonist of the adrenalin-beta3 receptor, SR59230A, enhances the Qui-induced inhibition of the contraction in the jejunum.},
     year = {2018}
    }
    

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    T1  - Differential Inhibition of the Rhythm and Amplitude of Acetylcholine-Dependent Contraction in the Murine Jejunum and Ileum In Vitro by Thiamin and Quinine
    AU  - Atsuko Yamashita
    AU  - Nana Shimamoto
    AU  - Kyoko Morita
    AU  - Hasumi Sugiyama
    AU  - Shiho Tadakuma
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    AU  - Mari Kimoto
    AU  - Kazuo Toda
    AU  - Masato Ota
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    JF  - Journal of Food and Nutrition Sciences
    JO  - Journal of Food and Nutrition Sciences
    SP  - 115
    EP  - 122
    PB  - Science Publishing Group
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    AB  - Previously, the effects of several bitter substances have been investigated in the contraction of the murine jejunum and ileum, reporting that these independently suppress the rhythm generation of the interstitial cells of Cajal. Recently, it was reported that thiamin, which binds to a bitter taste receptor, modifies the early phase of the ileum contraction, whereas the physiological effects on the rhythm and amplitude of jejunum and ileum contractions remain unclear. In this study, it was investigated the physiological effects of thiamin and quinine on the in vitro contraction of the murine jejunum and ileum using mice for all experiments. the periodic contraction of the jejunum was observed before the administration of acetylcholine (Ach) and other substances, and the tonic amplitudes induced by the substances. These bitter substances variably affect the Ach-induced rhythmic contraction of the jejunum and ileum in vitro. In addition, quinine hydrochloride (Qui) and thiamin hydrochloride (Thi) variably affect the Ach-induced rhythmic contraction of the jejunum and ileum in vitro. Both Qui and Thi markedly increase the rhythmic contraction in the jejunum. Although Thi does not change the rhythmic contraction in the ileum, it gradually reduces the amplitude in the jejunum. Conversely, Qui gradually reduces the amplitude and almost inhibits the contraction in the jejunum. Furthermore, an antagonist of the adrenalin-beta3 receptor, SR59230A, enhances the Qui-induced inhibition of the contraction in the jejunum.
    VL  - 6
    IS  - 5
    ER  - 

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