International Journal of Nutrition and Food Sciences

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Rat Hepatoma BRL Cells Proliferation by Acrylamide and Alcohol Combination

Received: 14 May 2020    Accepted: 29 May 2020    Published: 23 June 2020
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Abstract

Acrylamide, exists in carbohydrate-rich food heated at high temperatures, is a probable carcinogen to humans. Excessive alcohol intake can also lead to a variety of pathological changes in the body. Acrylamide in the foods and alcohol in the drinks are unavoidable. And studies have demonstrated that combination of the two substances which are taken into the body via diet may cause adverse effects in the cells, even induce impairments on testicular spermatogenesis in male offsprings. Both acrylamide and alcohol are mainly metabolized in the liver, where cytochrome P450 2E1 (CYP2E1) acts as the common metabolic enzyme of the two xenobiotics. This study aimed to explore the effects of acrylamide and alcohol combination on rat hepatoma BRL cells proliferation and the probable mechanisms. MTT, western blotting, EdU fluorescence staining, flow cytometry and PCR were used. Results showed that combination of acrylamide and alcohol at low doses promoted BRL cells proliferation through CYP2E1/Akt/ NF-κB/cyclinB1/cyclinD1 activation and ROS production. The combined effects of acrylamide and alcohol largely depended on ROS level. Furthermore, acrylamide and alcohol could synergistically induce miR-21, which was related to the progress of liver regeneration. These data showed that combination of acrylamide and alcohol at low doses promoted BRL cells proliferation through inducing ROS production, which indicated that intake of fried starch food with small dose of alcohol might have positive effects on liver regeneration.

DOI 10.11648/j.ijnfs.20200903.12
Published in International Journal of Nutrition and Food Sciences (Volume 9, Issue 3, May 2020)
Page(s) 78-85
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Acrylamide, Alcohol, Proliferation, ROS, miR-21

References
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[4] SCHETTGEN, T., K TTING, B., HORNIG, M., BECKMANN, M. W., WEISS, T., DREXLER, H., ANGERER, J. (2004). Trans-placental exposure of neonates to acrylamide—a pilot study. International Archives of Occupational & Environmental Health, 77: 213-216.
[5] EXON, J. H. (2006). A review of the toxicology of acrylamide. Journal of Toxicology & Environmental Health Part B Critical Reviews, 9: 397-412.
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[7] RONKSLEY, P. E., BRIEN, S. E., TURNER, B. J., MUKAMAL, K. J., GHALI, W. A. (2011). Association of alcohol consumption with selected cardiovascular disease outcomes: a systematic review and meta-analysis. Bmj, 342: d671.
[8] BRIEN, S. E., RONKSLEY, P. E., TURNER, B. J., MUKAMAL, K. J., GHALI, W. A. (2011). Effect of alcohol consumption on biological markers associated with risk of coronary heart disease: systematic review and meta-analysis of interventional studies. Bmj, 342: 480-480.
[9] SEN, E., TUNALI, Y., ERKAN, M. (2015). Testicular development of male mice offsprings exposed to acrylamide and alcohol during the gestation and lactation period. Human & Experimental Toxicology, 34: 401-14.
[10] SHAN, X. Y., LI, Y., MENG, X. L., WANG, P. Q., JIANG, P., FENG, Q. (2014). Curcumin and (-)-epigallocatechin-3-gallate attenuate acrylamide-induced proliferation in HepG2 cells. Food & Chemical Toxicology An International Journal Published for the British Industrial Biological Research Association, 66: 194-202.
[11] SUN, H. Z., YANG, T. W., ZANG, W. J., WU, S. F. (2010). Dehydroepiandrosterone-induced proliferation of prostatic epithelial cell is mediated by NFKB via PI3K/AKT signaling pathway. Journal of Endocrinology, 204: 311-318.
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[14] MARQUEZ, R. T., WENDLANDT, E., GALLE, C. S., KECK, K., MCCAFFREY, A. P. (2010). MicroRNA-21 is upregulated during the proliferative phase of liver regeneration, targets Pellino-1, and inhibits NF-kappaB signaling. American Journal of Physiology Gastrointestinal & Liver Physiology, 298: 535-41.
[15] NG, R., SONG, G., ROLL, G. R. (2012). A microRNA-21 surge facilitates rapid cyclin D1 translation and cell cycle progression in mouse liver regeneration. Journal of Clinical Investigation, 122: 1097-108.
[16] LI, J. J., CHAN, W. H., LEUNG, W. Y., WANG, Y., XU, C. S. (2015). MicroRNA-21 promotes proliferation of rat hepatocyte BRL-3A by targeting FASLG. Genetics & Molecular Research Gmr, 14: 4150-4160.
[17] WOLFGANG E, T. (2006). Repression of cytochrome P450 activity in human hepatocytes in vitro by a novel hepatotrophic factor, augmenter of liver regeneration. The Journal of pharmacology and experimental therapeutics, 2.
[18] LAMY, E., V LKEL, Y., ROOS, P. H., KASSIE, F., MERSCH-SUNDERMANN, V. (2008). Ethanol enhanced the genotoxicity of acrylamide in human, metabolically competent HepG2 cells by CYP2E1 induction and glutathione depletion. International journal of hygiene and environmental health, 211: 74-81.
[19] SHI, X. M., ZHANG, G. J., CHANG, Z. S., WU, X. L. (2014). Viability Reduction of Melanoma Cells by Plasma Jet via Inducing G1/S and G2/M Cell Cycle Arrest and Cell Apoptosis. Plasma Science IEEE Transactions on, 42: 1640-1647.
[20] DAY, R. M., SUZUKI, Y. J. (2004). Cell proliferation, reactive oxygen and cellular glutathione. Dose Response, 3: 425-442.
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Author Information
  • School of Public Health, University of South China, Hengyang, China; Department of Nutrition and Food Hygiene, Nanjing Medical University, Nanjing, China

  • Lianyungang Health Inspection Bureau, Lianyungang, China

  • Department of Nutrition and Food Hygiene, Nanjing Medical University, Nanjing, China

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    Xiaoyun Shan, Pengqi Wang, Qing Feng. (2020). Rat Hepatoma BRL Cells Proliferation by Acrylamide and Alcohol Combination. International Journal of Nutrition and Food Sciences, 9(3), 78-85. https://doi.org/10.11648/j.ijnfs.20200903.12

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    ACS Style

    Xiaoyun Shan; Pengqi Wang; Qing Feng. Rat Hepatoma BRL Cells Proliferation by Acrylamide and Alcohol Combination. Int. J. Nutr. Food Sci. 2020, 9(3), 78-85. doi: 10.11648/j.ijnfs.20200903.12

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    AMA Style

    Xiaoyun Shan, Pengqi Wang, Qing Feng. Rat Hepatoma BRL Cells Proliferation by Acrylamide and Alcohol Combination. Int J Nutr Food Sci. 2020;9(3):78-85. doi: 10.11648/j.ijnfs.20200903.12

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  • @article{10.11648/j.ijnfs.20200903.12,
      author = {Xiaoyun Shan and Pengqi Wang and Qing Feng},
      title = {Rat Hepatoma BRL Cells Proliferation by Acrylamide and Alcohol Combination},
      journal = {International Journal of Nutrition and Food Sciences},
      volume = {9},
      number = {3},
      pages = {78-85},
      doi = {10.11648/j.ijnfs.20200903.12},
      url = {https://doi.org/10.11648/j.ijnfs.20200903.12},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.ijnfs.20200903.12},
      abstract = {Acrylamide, exists in carbohydrate-rich food heated at high temperatures, is a probable carcinogen to humans. Excessive alcohol intake can also lead to a variety of pathological changes in the body. Acrylamide in the foods and alcohol in the drinks are unavoidable. And studies have demonstrated that combination of the two substances which are taken into the body via diet may cause adverse effects in the cells, even induce impairments on testicular spermatogenesis in male offsprings. Both acrylamide and alcohol are mainly metabolized in the liver, where cytochrome P450 2E1 (CYP2E1) acts as the common metabolic enzyme of the two xenobiotics. This study aimed to explore the effects of acrylamide and alcohol combination on rat hepatoma BRL cells proliferation and the probable mechanisms. MTT, western blotting, EdU fluorescence staining, flow cytometry and PCR were used. Results showed that combination of acrylamide and alcohol at low doses promoted BRL cells proliferation through CYP2E1/Akt/ NF-κB/cyclinB1/cyclinD1 activation and ROS production. The combined effects of acrylamide and alcohol largely depended on ROS level. Furthermore, acrylamide and alcohol could synergistically induce miR-21, which was related to the progress of liver regeneration. These data showed that combination of acrylamide and alcohol at low doses promoted BRL cells proliferation through inducing ROS production, which indicated that intake of fried starch food with small dose of alcohol might have positive effects on liver regeneration.},
     year = {2020}
    }
    

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  • TY  - JOUR
    T1  - Rat Hepatoma BRL Cells Proliferation by Acrylamide and Alcohol Combination
    AU  - Xiaoyun Shan
    AU  - Pengqi Wang
    AU  - Qing Feng
    Y1  - 2020/06/23
    PY  - 2020
    N1  - https://doi.org/10.11648/j.ijnfs.20200903.12
    DO  - 10.11648/j.ijnfs.20200903.12
    T2  - International Journal of Nutrition and Food Sciences
    JF  - International Journal of Nutrition and Food Sciences
    JO  - International Journal of Nutrition and Food Sciences
    SP  - 78
    EP  - 85
    PB  - Science Publishing Group
    SN  - 2327-2716
    UR  - https://doi.org/10.11648/j.ijnfs.20200903.12
    AB  - Acrylamide, exists in carbohydrate-rich food heated at high temperatures, is a probable carcinogen to humans. Excessive alcohol intake can also lead to a variety of pathological changes in the body. Acrylamide in the foods and alcohol in the drinks are unavoidable. And studies have demonstrated that combination of the two substances which are taken into the body via diet may cause adverse effects in the cells, even induce impairments on testicular spermatogenesis in male offsprings. Both acrylamide and alcohol are mainly metabolized in the liver, where cytochrome P450 2E1 (CYP2E1) acts as the common metabolic enzyme of the two xenobiotics. This study aimed to explore the effects of acrylamide and alcohol combination on rat hepatoma BRL cells proliferation and the probable mechanisms. MTT, western blotting, EdU fluorescence staining, flow cytometry and PCR were used. Results showed that combination of acrylamide and alcohol at low doses promoted BRL cells proliferation through CYP2E1/Akt/ NF-κB/cyclinB1/cyclinD1 activation and ROS production. The combined effects of acrylamide and alcohol largely depended on ROS level. Furthermore, acrylamide and alcohol could synergistically induce miR-21, which was related to the progress of liver regeneration. These data showed that combination of acrylamide and alcohol at low doses promoted BRL cells proliferation through inducing ROS production, which indicated that intake of fried starch food with small dose of alcohol might have positive effects on liver regeneration.
    VL  - 9
    IS  - 3
    ER  - 

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