International Journal of Nutrition and Food Sciences

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Lactulose Crystals Beneficially Affect Community Composition Along Entire Human Colon in Vitro, Resulting in Donor-Dependent Prebiotic Effects at Metabolic Level

Received: 12 February 2019    Accepted: 20 March 2019    Published: 29 April 2019
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Abstract

A validated in vitro gut model (i.e. SHIME®) was used to assess the effect of repeated daily administration of lactulose on microbial metabolic activity and community composition in different colonic areas, with the focus on inter-individual differences among three human subjects. An initial rise in acetate and lactate levels was observed in the proximal colon after lactulose administration, which could be linked to an overall strong bifidogenic effect as well as higher Lactobacilli levels in donors 2 and 3. Particularly two operational taxonomic units (OTUs) related to Bifidobacterium adolescentis and Bifidobacterium longum increased with lactulose addition. The enhanced acetate and lactate production subsequently stimulated microbial species involved in cross-feeding interactions, resulting in the donor-dependent production of propionate and/or butyrate. Additionally, a reduction in markers of proteolytic fermentation was detected upon lactulose supplementation. A wide spectrum of propionate- and especially butyrate-producing microbes, such as the next-generation probiotics Faecalibacterium prausnitzii and Akkermansia muciniphila, were donor-dependently enhanced in the distal colon, which is of specific interest as many colonic diseases originate in the distal part of the colon. For the first time beneficial effects of lactulose on the microbiota as well as metabolic activity could be demonstrated over the entire colon in vitro.

DOI 10.11648/j.ijnfs.20190801.12
Published in International Journal of Nutrition and Food Sciences (Volume 8, Issue 1, January 2019)
Page(s) 10-22
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Bifidobacteria, Faecalibacterium, Akkermansia, SHIME®, Microbial

References
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Author Information
  • Pro Digest Bvba, Ghent, Belgium

  • Pro Digest Bvba, Ghent, Belgium

  • Fresenius-Kabi Deutschland GmbH, Bad Homburg, Germany

  • Fresenius-Kabi Deutschland GmbH, Oberursel, Germany

  • Fresenius-Kabi Austria GmbH, Linz, Austria

  • Fresenius-Kabi Deutschland GmbH, Oberursel, Germany

  • Pro Digest Bvba, Ghent, Belgium; Center of Microbial Ecology and Technology (CMET), Ghent University, Ghent, Belgium

Cite This Article
  • APA Style

    Cindy Duysburgh, Pieter Van Den Abbeele, Melanie Bothe, John Stover, Angelika Kuchinka-Koch, et al. (2019). Lactulose Crystals Beneficially Affect Community Composition Along Entire Human Colon in Vitro, Resulting in Donor-Dependent Prebiotic Effects at Metabolic Level. International Journal of Nutrition and Food Sciences, 8(1), 10-22. https://doi.org/10.11648/j.ijnfs.20190801.12

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    ACS Style

    Cindy Duysburgh; Pieter Van Den Abbeele; Melanie Bothe; John Stover; Angelika Kuchinka-Koch, et al. Lactulose Crystals Beneficially Affect Community Composition Along Entire Human Colon in Vitro, Resulting in Donor-Dependent Prebiotic Effects at Metabolic Level. Int. J. Nutr. Food Sci. 2019, 8(1), 10-22. doi: 10.11648/j.ijnfs.20190801.12

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    AMA Style

    Cindy Duysburgh, Pieter Van Den Abbeele, Melanie Bothe, John Stover, Angelika Kuchinka-Koch, et al. Lactulose Crystals Beneficially Affect Community Composition Along Entire Human Colon in Vitro, Resulting in Donor-Dependent Prebiotic Effects at Metabolic Level. Int J Nutr Food Sci. 2019;8(1):10-22. doi: 10.11648/j.ijnfs.20190801.12

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  • @article{10.11648/j.ijnfs.20190801.12,
      author = {Cindy Duysburgh and Pieter Van Den Abbeele and Melanie Bothe and John Stover and Angelika Kuchinka-Koch and Susann Schwejda-Guettes and Massimo Marzorati},
      title = {Lactulose Crystals Beneficially Affect Community Composition Along Entire Human Colon in Vitro, Resulting in Donor-Dependent Prebiotic Effects at Metabolic Level},
      journal = {International Journal of Nutrition and Food Sciences},
      volume = {8},
      number = {1},
      pages = {10-22},
      doi = {10.11648/j.ijnfs.20190801.12},
      url = {https://doi.org/10.11648/j.ijnfs.20190801.12},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.ijnfs.20190801.12},
      abstract = {A validated in vitro gut model (i.e. SHIME®) was used to assess the effect of repeated daily administration of lactulose on microbial metabolic activity and community composition in different colonic areas, with the focus on inter-individual differences among three human subjects. An initial rise in acetate and lactate levels was observed in the proximal colon after lactulose administration, which could be linked to an overall strong bifidogenic effect as well as higher Lactobacilli levels in donors 2 and 3. Particularly two operational taxonomic units (OTUs) related to Bifidobacterium adolescentis and Bifidobacterium longum increased with lactulose addition. The enhanced acetate and lactate production subsequently stimulated microbial species involved in cross-feeding interactions, resulting in the donor-dependent production of propionate and/or butyrate. Additionally, a reduction in markers of proteolytic fermentation was detected upon lactulose supplementation. A wide spectrum of propionate- and especially butyrate-producing microbes, such as the next-generation probiotics Faecalibacterium prausnitzii and Akkermansia muciniphila, were donor-dependently enhanced in the distal colon, which is of specific interest as many colonic diseases originate in the distal part of the colon. For the first time beneficial effects of lactulose on the microbiota as well as metabolic activity could be demonstrated over the entire colon in vitro.},
     year = {2019}
    }
    

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  • TY  - JOUR
    T1  - Lactulose Crystals Beneficially Affect Community Composition Along Entire Human Colon in Vitro, Resulting in Donor-Dependent Prebiotic Effects at Metabolic Level
    AU  - Cindy Duysburgh
    AU  - Pieter Van Den Abbeele
    AU  - Melanie Bothe
    AU  - John Stover
    AU  - Angelika Kuchinka-Koch
    AU  - Susann Schwejda-Guettes
    AU  - Massimo Marzorati
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    T2  - International Journal of Nutrition and Food Sciences
    JF  - International Journal of Nutrition and Food Sciences
    JO  - International Journal of Nutrition and Food Sciences
    SP  - 10
    EP  - 22
    PB  - Science Publishing Group
    SN  - 2327-2716
    UR  - https://doi.org/10.11648/j.ijnfs.20190801.12
    AB  - A validated in vitro gut model (i.e. SHIME®) was used to assess the effect of repeated daily administration of lactulose on microbial metabolic activity and community composition in different colonic areas, with the focus on inter-individual differences among three human subjects. An initial rise in acetate and lactate levels was observed in the proximal colon after lactulose administration, which could be linked to an overall strong bifidogenic effect as well as higher Lactobacilli levels in donors 2 and 3. Particularly two operational taxonomic units (OTUs) related to Bifidobacterium adolescentis and Bifidobacterium longum increased with lactulose addition. The enhanced acetate and lactate production subsequently stimulated microbial species involved in cross-feeding interactions, resulting in the donor-dependent production of propionate and/or butyrate. Additionally, a reduction in markers of proteolytic fermentation was detected upon lactulose supplementation. A wide spectrum of propionate- and especially butyrate-producing microbes, such as the next-generation probiotics Faecalibacterium prausnitzii and Akkermansia muciniphila, were donor-dependently enhanced in the distal colon, which is of specific interest as many colonic diseases originate in the distal part of the colon. For the first time beneficial effects of lactulose on the microbiota as well as metabolic activity could be demonstrated over the entire colon in vitro.
    VL  - 8
    IS  - 1
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