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Determination of Microbial Growth and Survival in Salad Vegetables through in Vitro Challenge Test

Received: 4 November 2013    Accepted:     Published: 30 November 2013
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Abstract

Current study attempted to examine the growth and subsequent survival of the common spoilage bacteria in vegetable samples collected from Dhaka, Bangladesh. Carrot, cucumber, tomato and lettuce samples were obtained from local markets and rendered free of contaminants. Each sample was then inoculated separately with an array of 9 test bacteria, resulting in the initial load of 105 cfu/g. The results revealed more than 6-log reduction of Salmonella spp. in carrot and tomato samples, Shigella spp. in carrot, lettuce and cucumber samples, Aeromonas spp. in tomato samples, Pseudomonas spp. in lettuce samples, and Listeria spp. in cucumber samples. No significant reduction in E. coli was observed in the cucumber samples, while in carrots and tomato samples, approximately 2- log reductions was found. Demonstration of the capacity of vegetables to influence microbial growth would further aid in the maintenance of the food quality and stability as well as their shelf life. Conducting such experiments after the quantification of spoiling microorganisms thus imparts a complete bacteriological profile, which is of public health significance.

Published in International Journal of Nutrition and Food Sciences (Volume 2, Issue 6)
DOI 10.11648/j.ijnfs.20130206.18
Page(s) 312-319
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Vegetables, Microorganisms, Growth, Challenge Test, Consumer Safety

References
[1] Abadias, M., Usall, J., Anguera, M., Solsona, C. and Viñas I. "Microbiological quality of fresh, minimally processed fruit and vegetables, and sprouts from retail establishments", International Journal of Food Microbiology, vol. 123, No. 1-2, pp. 121-129, 2008.
[2] AFSSA (Agence Franc¸aise de Se´curite´ Sanitaire des Aliments) (2008), "Technical guidance document on shelf-life studies for Listeria monocytogenes in ready-to-eat foods", Available at: http://ec.europa.eu/food/food/biosafety/salmonella/docs/shelflife_ listeria_monocytogenes _en.pdf (Accessed 18 April 2013).
[3] Alfrad, E.B, Bensons Microbiological Applications, Mcgraw-Hill: New York, 2007.
[4] APHA (American Public Health Association), Standard Methods for the Examination of Water and Wastewater, American Public Health Association: Washington, D.C., 1998.
[5] Beuchat, L.R., "Pathogenic microorganisms associated with fresh produce", J. Food Prot., Vol. 59, No. 2, pp. 204-216, 1996.
[6] Bibek R., Fundamental of Food Microbiology, CRC press, Boca Raton: London New York Washington D.C., 2005.
[7] Burnett, S.L. and Beuchat, L.R., "Human pathogens associated with raw produce and unpasteurized juices, and difficulties in contamination", J. Indust. Microbiol. Biotechnol., Vol. 27, No. 2, pp. 104-110, 2001.
[8] Cappuccino, J.G. and Sherman, N., Microbiology- A Laboratory Manual, The Benjamin/Cummings Publishing Co. Inc.: California, 1996.
[9] Colwell, R.R., "Bacterial death revisited", in Colwell, R. R. and Grimes D. J. (Eds), Non-culturable microorganisms in the environment, American Society of Microbiology: Washington DC, USA, pp. 325-342, 2001.
[10] Cordano, A.M. and Jacquet, C., "Listeria monocytogenes isolated from vegetable salads sold at supermarkets in Santiago, Chile: Prevalence and strain characterization", International Journal of Food Microbiology, Vol. 132, No. 2-3, pp. 176-179, 2009.
[11] Cray, W.C.J. and Moon, W.H., "Experimental infection of calves and adult cattle with Escherichia coli O157:H7." Appl. Environ. Microbiol., Vol. 61, No. 4, pp. 1586-1590, 1995.
[12] Fain, A.R., "A review of the microbiological safety of fresh salads", Dairy, Food, and Environmental Sanitation, Vol. 16, No. 3, pp. 146-149, 1996.
[13] FDA (U.S. Food and Drug Administration) (2009), "Elimination of Microbial Hazards on Fresh and Fresh-Cut
[14] Produce", Chapter IV, Outbreaks Associated with Fresh and Fresh-Cut Produce. Incidence, Growth, and Survival of Pathogens in Fresh and Fresh-Cut Produce.
[15] FDA (U.S. Food and Drug Administration) (2001a), "Evaluation and Definition of Potentially Hazardous Foods", Chapter 3, Factors that Influence Microbial Growth, available at: http://www.fda.gov/Food/FoodScienceResearch/SafePracticesforFoodProcesses/ucm094145.htm (Accessed 25 April 2013).
[16] FDA (U.S. Food and Drug Administration) (2001b), "Evaluation and Definition of Potentially Hazardous Foods", Chapter 6, Microbiological Challenge Testing. Available at: http://www.fda.gov/Food/FoodScienceResearch/SafePracticesforFoodProcesses/ucm094154.htm (Accessed 25 April 2013).
[17] Gould, G.W., "Preservation: past, present and future", Br. Med. Bull., Vol, 56, No. 1, pp. 84–96, 2004.
[18] Guo, X., van Iersel, M.W., Chen, J., Brackett, R.E., and Beuchat, L. R. , "Evidence of association of salmonellae with tomato plants grown hydroponically in inoculated nutrient solution", Appl. Environ. Microbiol., Vol. 68, No. 7, pp. 3639-3643, 2002.
[19] Kabir, M.S., Yamashita, D., Noor, R. and Yamada, M., "Effect of σS on σE-directed cell lysis in Escherichia coli early stationary phase", J. Mol. Microbiol. Biotechnol., Vol. 8, No. 3, pp. 189–194, 2004.
[20] King, A.D. and Bolin, H.R., "Physiological and microbiological storage stability of minimally processed fruits and vegetables", Food Technol., Vol. 43, No. 2, pp. 132-135, 1989.
[21] Manou, I., Bouillard, L., Devleeschouwer, M.J. and Barel, A.O., "Evaluation of the preservative properties of Thymus vulgaris essential oil in topically applies formulations under a challenge test", Journal of Applied Microbiology, Vol. 84, No. 3, pp 368-367, 1988.
[22] NACMCF (National Advisory Committee on Microbiological Criteria for Foods), "Parameters for Determining Inoculated Pack/Challenge Study Protocols", Journal of Food Protection, Vol. 73, No. 1, pp. 140–202, 2010.
[23] Nipa, M.N., Mazumdar, R.M., Hasan, M.M., Fakruddin, M., Islam, S., Bhuiyan, H.R. and Iqbal, A., "Prevalence of Multi Drug Resistant Bacteria on Raw Salad Vegetables Sold in Major Markets of Chittagong City, Bangladesh", Middle East Journal of Scientific Research, Vol. 10, No. 1, pp. 70-11, 2011.
[24] Noor, R., Murata, M. and Yamada, M., "Oxidative Stress as a Trigger for Growth Phase-Specific σE - Dependent Cell Lysis in Escherichia coli", J. Mol. Microbiol. Biotechnol, Vol. 17, pp. 177–187, 2009b.
[25] Noor, R., Murata, M., Nagamitsu, H., Klein, G., Raina, S. and Yamada, M., "Dissection of σE -dependent cell lysis in Escherichia coli: roles of RpoE regulators RseA, RseB and periplasmic folding catalyst PpiD", Genes to Cells, Vol. 14, pp. 885–899, 2009a.
[26] Notermans, S. and Veld, P., "Microbiological challenge testing for ensuring safety of food products", International Journal of Food Microbiology, Vol. 24, No. 1-2, pp. 33-39, 1994.
[27] Notermans, S., Veld, P., Wijtzes, T. and Mead G.C., "A user's guide to microbiological challenge testing for ensuring the safety and stability of food products", Food Microbiol, Vol. 10, No. 2, pp. 145-57, 1993.
[28] Rahman, F. and Noor, R., "Prevalence of pathogenic bacteria in common salad vegetables of Dhaka metropolis", Bangladesh Journal of Botany, Vol. 41, No. 2, pp. 159-162, 2012.
[29] Robbs, P.G., Bartz, J.A., McFie G. and Hodge N. C., "Causes of decay of fresh-cut celery", Journal of Food Science, Vol. 61, No. 2, pp. 444–448, 1996.
[30] Scott, V.N., Swanson, K.M. J., Freier, T.A., Pruett, W.P., Sveum, W.H., Hall, P.A., Smoot, L. A. and. Brown D. G., "Guidelines for conducting Listeria monocytogenes challenge testing of foods", Food Prot. Trends Vol. 25, No. 11, pp. 818–825, 2005.
[31] Solomon, E.B., Yaron, S. and Matthews, K.R., "Transmission of Escherichia coli O157:H7 from contaminated manure and irrigation water to lettuce plant tissue and its subsequent internalization", Applied Environmental Microbiology, Vol. 68, No. 1, pp. 397-400, 2002.
[32] Southon, S., "Increased fruit and vegetable consumption within the EU: potential health benefits", Food Res. Intl., Vol. 33, No. 3-4, pp. 211-217, 2000.
[33] Starutch, D., "Survival of pathogenic microorganisms and parasites in excreta, manure sand sewage sludge", Rev. Sci. Tech. Off. Int. Epiz., Vol. 10, No. 3, pp. 813-846, 1991.
[34] Wachtel, M.R., Whitehand, L.C. and Mandrell, R.E., "Association of Escherichia coli O157:H7 with preharvest leaf lettuce upon exposure to contaminated irrigation water", Journal of Food Protection, Vol. 65, No.1, pp. 18-25, 2002.
[35] Wargovich, M.J., "Anticancer properties of fruits and vegetables", Horticulture Science, Vol. 35, No. 4, pp. 573-575, 2000.
[36] Zepp, G., Kuchler, F. and Lucier, G., "Food safety and fresh fruits and vegetables: is there a difference between imported and domestically produced products?" Vegetables and Specialties, Situation and Outlook Report, Vol. 274, pp. 23-28, 1998.
Cite This Article
  • APA Style

    Farahnaaz Feroz, Jessica Das Senjuti, Rashed Noor. (2013). Determination of Microbial Growth and Survival in Salad Vegetables through in Vitro Challenge Test. International Journal of Nutrition and Food Sciences, 2(6), 312-319. https://doi.org/10.11648/j.ijnfs.20130206.18

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    ACS Style

    Farahnaaz Feroz; Jessica Das Senjuti; Rashed Noor. Determination of Microbial Growth and Survival in Salad Vegetables through in Vitro Challenge Test. Int. J. Nutr. Food Sci. 2013, 2(6), 312-319. doi: 10.11648/j.ijnfs.20130206.18

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    AMA Style

    Farahnaaz Feroz, Jessica Das Senjuti, Rashed Noor. Determination of Microbial Growth and Survival in Salad Vegetables through in Vitro Challenge Test. Int J Nutr Food Sci. 2013;2(6):312-319. doi: 10.11648/j.ijnfs.20130206.18

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  • @article{10.11648/j.ijnfs.20130206.18,
      author = {Farahnaaz Feroz and Jessica Das Senjuti and Rashed Noor},
      title = {Determination of Microbial Growth and Survival in Salad Vegetables through in Vitro Challenge Test},
      journal = {International Journal of Nutrition and Food Sciences},
      volume = {2},
      number = {6},
      pages = {312-319},
      doi = {10.11648/j.ijnfs.20130206.18},
      url = {https://doi.org/10.11648/j.ijnfs.20130206.18},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijnfs.20130206.18},
      abstract = {Current study attempted to examine the growth and subsequent survival of the common spoilage bacteria in vegetable samples collected from Dhaka, Bangladesh. Carrot, cucumber, tomato and lettuce samples were obtained from local markets and rendered free of contaminants. Each sample was then inoculated separately with an array of 9 test bacteria, resulting in the initial load of 105 cfu/g. The results revealed more than 6-log reduction of Salmonella spp. in carrot and tomato samples, Shigella spp. in carrot, lettuce and cucumber samples, Aeromonas spp. in tomato samples, Pseudomonas spp. in lettuce samples, and Listeria spp. in cucumber samples. No significant reduction in E. coli was observed in the cucumber samples, while in carrots and tomato samples, approximately 2- log reductions was found. Demonstration of the capacity of vegetables to influence microbial growth would further aid in the maintenance of the food quality and stability as well as their shelf life. Conducting such experiments after the quantification of spoiling microorganisms thus imparts a complete bacteriological profile, which is of public health significance.},
     year = {2013}
    }
    

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  • TY  - JOUR
    T1  - Determination of Microbial Growth and Survival in Salad Vegetables through in Vitro Challenge Test
    AU  - Farahnaaz Feroz
    AU  - Jessica Das Senjuti
    AU  - Rashed Noor
    Y1  - 2013/11/30
    PY  - 2013
    N1  - https://doi.org/10.11648/j.ijnfs.20130206.18
    DO  - 10.11648/j.ijnfs.20130206.18
    T2  - International Journal of Nutrition and Food Sciences
    JF  - International Journal of Nutrition and Food Sciences
    JO  - International Journal of Nutrition and Food Sciences
    SP  - 312
    EP  - 319
    PB  - Science Publishing Group
    SN  - 2327-2716
    UR  - https://doi.org/10.11648/j.ijnfs.20130206.18
    AB  - Current study attempted to examine the growth and subsequent survival of the common spoilage bacteria in vegetable samples collected from Dhaka, Bangladesh. Carrot, cucumber, tomato and lettuce samples were obtained from local markets and rendered free of contaminants. Each sample was then inoculated separately with an array of 9 test bacteria, resulting in the initial load of 105 cfu/g. The results revealed more than 6-log reduction of Salmonella spp. in carrot and tomato samples, Shigella spp. in carrot, lettuce and cucumber samples, Aeromonas spp. in tomato samples, Pseudomonas spp. in lettuce samples, and Listeria spp. in cucumber samples. No significant reduction in E. coli was observed in the cucumber samples, while in carrots and tomato samples, approximately 2- log reductions was found. Demonstration of the capacity of vegetables to influence microbial growth would further aid in the maintenance of the food quality and stability as well as their shelf life. Conducting such experiments after the quantification of spoiling microorganisms thus imparts a complete bacteriological profile, which is of public health significance.
    VL  - 2
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    ER  - 

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Author Information
  • Dept. of Microbiology, Stamford University Bangladesh, Dhaka, Bangladesh

  • Dept. of Microbiology, Stamford University Bangladesh, Dhaka, Bangladesh

  • Dept. of Microbiology, Stamford University Bangladesh, Dhaka, Bangladesh

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