The effect of high fat diet on lipid and carbohydrate metabolic changes was studied in mature male albino rats. The animals were rendered obese by feeding a high fat diet for 60 days. Half of obese rats were become diabetic by injecting with a reduced dose of alloxan; to produce a non insulin dependent diabetes mellitus state. These obese models of non diabetic and diabetic rats were compared with normal male rats kept on a basal diet for 60 days. The fat fed rats beside gaining weight showed a significant rise in their hepatic lipid contents and observed an insulin resistant state. Lipid profile of the omental adipose tissue exhibited a significantly higher total lipid content, but lower total cholesterol, triglycerides and phospholipids contents in both obese (non diabetic and diabetic) as compared with the normal rats. On the other hand, the brown adipose tissue showed a reduced fat catabolism with a significant rise in its phospholipid contents. Correlation between plasma atherogenic index and plasma fatty acids pattern showed that in the obese diabetic rats atherogenic index was positively correlated with total polyunsaturated fatty acids, while significantly negative correlation with total saturated fatty acids. This work showed that high fat substrates have been got remarkable impacts on rats body weight and metabolism.
| Published in | International Journal of Nutrition and Food Sciences (Volume 2, Issue 2) |
| DOI | 10.11648/j.ijnfs.20130202.11 |
| Page(s) | 24-30 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2024. Published by Science Publishing Group |
Obesity, Diabetes, Male Albino Rats
| [1] | M. Croze, R. Vella, N. Pillon, H. Soula, L. Hadji, M. Gui-chardant, and C. Soulage, "Chronic treatment with myo-inositol reduces white adipose tissue accretion and im-proves insulin sensitivity in female mice", The Journal of Nutritional Biochemistry, 2013, 24(2): 457–466. |
| [2] | T. Pischon, H. Boeing, and K. Hffmann, "General and ab-dominal adiposity and risk of death in Europe", N. Engl. J. Med. 2008, 359(20): 2105-2120. |
| [3] | L. Storlien, A. Jenkkins, D. Chisholm, W. Pascoe, S. Khouri, and E. Kraegen, (1991): "Influence of dietary fat composition on development of insulin resistance in rats relationship to muscle triglycerides and n-3 fatty acids in muscles phospho-lipids", Diabetes, 40: 280-289. |
| [4] | T. Pelinkanova, M. Kohout, J. Valek, J. Base, and L. Kazdova, "Insulin secretion and insulin action related to the serum phospholipids fatty acids pattern in healthy men", Metabolism, 1989, 38(2): 188-192. |
| [5] | E. Berry, J. Hirsch, J. Most, D. McNamara, and J. Thornton, "The relationship of dietary fat to plasma lipid levels as studied by factor analysis of adipose tissue fatty acid composition in a free-living population of middle aged American men", Am. J. Clin. Nutr. 1986, 44: 220-231. |
| [6] | J. Triscari, N. Koral, B. Levin, and A. Sullivan, "Changes in lipid metabolism in diet-induced obesity", Metabolism 1985, 34(6): 580-587. |
| [7] | M. Osfor, "Protective role of green Tea and Panax ginseng in cancer-promoting Diets: a sub-chronic study on the male albino rats", Bull. NRC, Egypt, 2003, 28(5): 591 – 621. |
| [8] | J. Folch, and G. Stanley, "A simple method for the isolation and purification of total lipids from animal tissue", J. boil. Chem. 1957, 226: 497. |
| [9] | G. Siest, J. Henny, and F. Schiele, (1981): Interpretation des examens de Laboratorire. Karger ed. pp: 206-223. |
| [10] | R. Yalow, and W. Bouman, Plasma Insulin in Health and Disease. In: Diabetes mellitus: Theory and Practice, M. Edinberg and H. Rifkin (Eds.), Excerpta Medica, New York, pp: 119-150, 1983. |
| [11] | J. Schmit, and B. Drevon, "Sulfo-phospho-Vanillic reaction in lipid and lipoprotein evaluation in baboon serum comparison with human serum", Compt. Rend. Soc. Boil. 1964, 158(4), 778-780. |
| [12] | V. Dole, "Fractionation of plasma non- esterifies fatty acids Proc", Soc. Exptl. Biol. Med. 1956, 93: 532-533. |
| [13] | P. Fossati, and L. Prencipe, "Serum triglycerides determined calorimetrically with an enzyme that produces hydrogen peroxide", Clin. Chem. 1982, 28: 2077. |
| [14] | E. King, and I. Woolton, Microanalysis in Medical Bioche-mistry, 3rd ed. Churchill LTD: London, 1956. |
| [15] | C. Allain, L. Poon, C. Chan, W. Richmond, and P. Fu, "En-zymatic determination of total cholesterol", J. Clin. Chem. 1974, 20:470-475. |
| [16] | C. Ratliff, and F. Hall, Laboratory Manual of Clinical Bio-chemistry. USA, Scott and White Memorial Hospital Publi-cations Office Temple, IX, 1977. |
| [17] | M. Lopez-Virella, P. Stoine, S. Ellis, and J. Acollwall, "Cholesterol determination in high density lipoprotein by three different methods", Clin. Chem. 1977, 53(5): 882-884. |
| [18] | T. Glatter, "Hyperlipidemia, What is normal, who should be treated and how?", Post-Grad Med. 1984, 76(6): 49-59. |
| [19] | P. Armitage, "Statistical Methods in Medical Research", 3rd ed. Blackwell scientific Publications, Oxford and Einburgh, England, 1974. |
| [20] | C. Lavie, R. Milani, and H. Ventura, "Obesity and cardi-ovascular disease; risk factor, Paradox, and impact of weight loss", J. Am. Coll. Cardiol. 2009, 53: 1925-1932. |
| [21] | S. Grundy, J. Cleeman, and R. Daniels, "Diagnosis and management of the metabolic syndrome, Circulation, 2005, 112: 2735-3752. |
| [22] | S. Grundy, B. Hansen, and Jr S. Smith, "American heart association; national heart, Lung and blood Institute; American diabetes association 2004 Clinical management of the metabolic syndrome: report of the American Heart association/ National heart, lund, and Blood institute/ American Diabetes association Conference on scientific issues related to management", Circulation, 2004, 109: 551-556. |
| [23] | J. Baker, L. Olsen, and T. Soprensen, "Childhood body-mass index and the risk of coronary heart disease in adulthood", N. Engl. J. Med. 2007, 357: 2329-2337. |
| [24] | A. Oreopoulos, R. Paswal, and K. Kalantar-zadeh, "Body mass index and mortality in heart failure: a meta analysis", Am. Heart J. 2008, 156: 13-22. |
| [25] | L. Barness, J. Opitz, and E. Gilbert-Barness, "Obesity: genetic, molecular, and environmental aspects", Am. J. Med. Genet. 2007, 143A (24): 3016-34. |
| [26] | T. Constantine, H. Vojtech, B. Arnaud, F. Nick, F. Martin, M. Elisabeth, M. Dragan, and M. Mximo, "Management of ob-esity in adults: European Clinical Practice Guidelines", The European Journal of Obesity, 2008, 1(2): 106-116. |
| [27] | R. Ness-Abramof, and C. Apovian, "Diet modification for treatment and prevention of obesity", Endocrine, 2006, 29 (1): 5-9. |
| [28] | P. De Varies, H. Folkers, and C. Defijter, "Adipose tissue fatty acid composition and its relation to diet and plasma lipid concentrations in haemodialysis patients", Am. Clin. Nutr. 1991, 53:468-473. |
| [29] | G. Whitlock, S. Lewington, and P. Sherliker, "Body mass index and cause specific mortality in 900 000 adults: Colla-borative analysis of 57 prospective studies", Lancet, 2009, 373: 1083-1096. |
| [30] | R. Brownson, T. Boehmer, and D. Luke, "Declining rates of physical activity in the united states: what are the contributors?", Annu Rev. public Health, 2005, 26: 421-443. |
| [31] | K. Borodulin, T. Laatikainen, A. Juolevi, and P. Jousilahti, "Thirsty year trends of physical activity in relation to age, calendar time and birth cohort in finnish adults", Eur. J. Public Health, 2008, 18 (3): 339-344. |
| [32] | M. Fried, V. Hainer, and A. Basdevnt, "Inter-disciplinary European guidelines on surgery of severs obesity", Int. J. Obes. (Lond), 2007, 31(4): 569-577. |
| [33] | I. Cusin, F. Rohner-Jeanreenaud, J. Jenettaz, and B. Jeanrenaud, "Hyperinsulinemia and its impact on obesity and insulin resistance", Int. JK, 1992, Obesity 16 (4), SI-SII. |
| [34] | W. Mckane, A. Stevers, R. Woods, W. Andrews, R. Henry, and P. Bell, "The assessment of hepatic and peripheral insulin sensitivity in htpertriglyceridemia", Metabolism, 1990, 39 (12), 1240-1245. |
| [35] | R. Harris, and H. Kor, "Insulin insensitivity is rapidly reversed in rats by reucing dietary fat from 40 to 30 % of energy", J Nutr. 1992, 122: 1811-1822. |
| [36] | R. Krauss, M. Winston, B. Fletcher, and S. Graundy, "Obesity is not an independent risk factor for adverse peri-operative and long-term Clinical outcomes following Open AAA Repair or Evar VASC", Endovascular Surg. 2011, 45: 607-613. |
| [37] | E. Schaefer, R. Levy, N. Renst, F. Van sant, and H. Brewer, "The effect of low cholesterol, high polyunsaturated fat, and low fat diet on plasma lipids and cholesterol levels in normal and hypercholesterolemic subjects", Am. J. Clin. Nutr. 1981, 34, 1758-1763. |
| [38] | M. Osfor, W. Nicola, E. Badawy, and M. Emam, "Composition of plasma lipoproteins and their impact on metabolic complication in fatness rats treated with metformin and ni-cotinic acid", Med. J. Cairo Univ., 1999, 67(1) (suppl.) 59 – 73. |
APA Style
Mostafa M. Osfor, Osama Kensarah, Firas Azzeh, Mohammad A. Elmadbouly, Mona El-Qutry. (2013). Biochemical Changes in LIPID and Car-bohydrate Metabolism in Obese Diabetic Male Albino Rats. International Journal of Nutrition and Food Sciences, 2(2), 24-30. https://doi.org/10.11648/j.ijnfs.20130202.11
ACS Style
Mostafa M. Osfor; Osama Kensarah; Firas Azzeh; Mohammad A. Elmadbouly; Mona El-Qutry. Biochemical Changes in LIPID and Car-bohydrate Metabolism in Obese Diabetic Male Albino Rats. Int. J. Nutr. Food Sci. 2013, 2(2), 24-30. doi: 10.11648/j.ijnfs.20130202.11
AMA Style
Mostafa M. Osfor, Osama Kensarah, Firas Azzeh, Mohammad A. Elmadbouly, Mona El-Qutry. Biochemical Changes in LIPID and Car-bohydrate Metabolism in Obese Diabetic Male Albino Rats. Int J Nutr Food Sci. 2013;2(2):24-30. doi: 10.11648/j.ijnfs.20130202.11
Share This Article
Twitter
Linked In
Facebook
Copy
Download@article{10.11648/j.ijnfs.20130202.11,
author = {Mostafa M. Osfor and Osama Kensarah and Firas Azzeh and Mohammad A. Elmadbouly and Mona El-Qutry},
title = {Biochemical Changes in LIPID and Car-bohydrate Metabolism in Obese Diabetic Male Albino Rats},
journal = {International Journal of Nutrition and Food Sciences},
volume = {2},
number = {2},
pages = {24-30},
doi = {10.11648/j.ijnfs.20130202.11},
url = {https://doi.org/10.11648/j.ijnfs.20130202.11},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijnfs.20130202.11},
abstract = {The effect of high fat diet on lipid and carbohydrate metabolic changes was studied in mature male albino rats. The animals were rendered obese by feeding a high fat diet for 60 days. Half of obese rats were become diabetic by injecting with a reduced dose of alloxan; to produce a non insulin dependent diabetes mellitus state. These obese models of non diabetic and diabetic rats were compared with normal male rats kept on a basal diet for 60 days. The fat fed rats beside gaining weight showed a significant rise in their hepatic lipid contents and observed an insulin resistant state. Lipid profile of the omental adipose tissue exhibited a significantly higher total lipid content, but lower total cholesterol, triglycerides and phospholipids contents in both obese (non diabetic and diabetic) as compared with the normal rats. On the other hand, the brown adipose tissue showed a reduced fat catabolism with a significant rise in its phospholipid contents. Correlation between plasma atherogenic index and plasma fatty acids pattern showed that in the obese diabetic rats atherogenic index was positively correlated with total polyunsaturated fatty acids, while significantly negative correlation with total saturated fatty acids. This work showed that high fat substrates have been got remarkable impacts on rats body weight and metabolism.},
year = {2013}
}
TY - JOUR T1 - Biochemical Changes in LIPID and Car-bohydrate Metabolism in Obese Diabetic Male Albino Rats AU - Mostafa M. Osfor AU - Osama Kensarah AU - Firas Azzeh AU - Mohammad A. Elmadbouly AU - Mona El-Qutry Y1 - 2013/03/10 PY - 2013 N1 - https://doi.org/10.11648/j.ijnfs.20130202.11 DO - 10.11648/j.ijnfs.20130202.11 T2 - International Journal of Nutrition and Food Sciences JF - International Journal of Nutrition and Food Sciences JO - International Journal of Nutrition and Food Sciences SP - 24 EP - 30 PB - Science Publishing Group SN - 2327-2716 UR - https://doi.org/10.11648/j.ijnfs.20130202.11 AB - The effect of high fat diet on lipid and carbohydrate metabolic changes was studied in mature male albino rats. The animals were rendered obese by feeding a high fat diet for 60 days. Half of obese rats were become diabetic by injecting with a reduced dose of alloxan; to produce a non insulin dependent diabetes mellitus state. These obese models of non diabetic and diabetic rats were compared with normal male rats kept on a basal diet for 60 days. The fat fed rats beside gaining weight showed a significant rise in their hepatic lipid contents and observed an insulin resistant state. Lipid profile of the omental adipose tissue exhibited a significantly higher total lipid content, but lower total cholesterol, triglycerides and phospholipids contents in both obese (non diabetic and diabetic) as compared with the normal rats. On the other hand, the brown adipose tissue showed a reduced fat catabolism with a significant rise in its phospholipid contents. Correlation between plasma atherogenic index and plasma fatty acids pattern showed that in the obese diabetic rats atherogenic index was positively correlated with total polyunsaturated fatty acids, while significantly negative correlation with total saturated fatty acids. This work showed that high fat substrates have been got remarkable impacts on rats body weight and metabolism. VL - 2 IS - 2 ER -
Information
Email: