Spectrum and Drug Susceptibility Profile of Bacteria Recovered from Patients with Wound Infection Referred to Arsho Advanced Medical Laboratory

Adane Bitew; Mesele Admassie; Tigist Getachew

doi:doi:10.11648/j.cmr.20180701.12

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Spectrum and Drug Susceptibility Profile of Bacteria Recovered from Patients with Wound Infection Referred to Arsho Advanced Medical Laboratory

Adane Bitew, Mesele Admassie, Tigist Getachew

Published in Clinical Medicine Research (Volume 7, Issue 1)

Received: 4 January 2018 Accepted: 9 February 2018 Published: 7 March 2018

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Abstract

Wound infection still remains a significant cause of morbidly and mortality. Hence, studying the spectrum of bacterial etiological agents and their drug susceptibility profile is critical. A prospective study was conducted at Arsho Advanced Medical Laboratory from June 2016 to July 2017. Wound specimens were collected from 366 patients following standard procedures. Specimens were plated and incubated at 37°C for 48 hours. Identification and drug susceptibility testing of cultures were carried out by using the VITEK 2 compact system. Among 366 wound samples cultured, bacteria grew in 271(74%) samples. The highest (81.9%) wound infections were documented among patients with an age group of 15-64 years. Two hundred twenty one bacterial isolates were recovered of which 43.2% were Gram-negative while, 56.8% were Gram-positive. Staphylococcus aureus and Coagulase-Negative Staphylococci were major Gram-positive bacteria while Escherichia coli and Pseudomonas spp. were the commonest Gram-negative bacteria. Gram-negative bacteria had the highest overall drug resistance rate against ampicillin. Tobramycin and piperacillin/tazobactam combination were effective antimicrobial agents against Gram-negative bacteria. The highest overall resistance rate to Gram-positive bacteria was observed against erythromycin. Vancomycin and linezolid were the most active antimicrobial agents against Gram-positive bacteria. High culture positivity rate of wound infections reported in the present study initiates many similar studies to be conducted on wound in the country. High level of drug resistance to the commonly prescribed drugs dictates a search for better choices.

Published in	Clinical Medicine Research (Volume 7, Issue 1)
DOI	10.11648/j.cmr.20180701.12
Page(s)	8-17
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Wound Infections, Drug Suscetibity Pattern, Etiological Agents, Ethiopia

References

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APA Style

Adane Bitew, Mesele Admassie, Tigist Getachew. (2018). Spectrum and Drug Susceptibility Profile of Bacteria Recovered from Patients with Wound Infection Referred to Arsho Advanced Medical Laboratory. Clinical Medicine Research, 7(1), 8-17. https://doi.org/10.11648/j.cmr.20180701.12

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ACS Style

Adane Bitew; Mesele Admassie; Tigist Getachew. Spectrum and Drug Susceptibility Profile of Bacteria Recovered from Patients with Wound Infection Referred to Arsho Advanced Medical Laboratory. Clin. Med. Res. 2018, 7(1), 8-17. doi: 10.11648/j.cmr.20180701.12

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AMA Style

Adane Bitew, Mesele Admassie, Tigist Getachew. Spectrum and Drug Susceptibility Profile of Bacteria Recovered from Patients with Wound Infection Referred to Arsho Advanced Medical Laboratory. Clin Med Res. 2018;7(1):8-17. doi: 10.11648/j.cmr.20180701.12

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@article{10.11648/j.cmr.20180701.12,
  author = {Adane Bitew and Mesele Admassie and Tigist Getachew},
  title = {Spectrum and Drug Susceptibility Profile of Bacteria Recovered from Patients with Wound Infection Referred to Arsho Advanced Medical Laboratory},
  journal = {Clinical Medicine Research},
  volume = {7},
  number = {1},
  pages = {8-17},
  doi = {10.11648/j.cmr.20180701.12},
  url = {https://doi.org/10.11648/j.cmr.20180701.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.cmr.20180701.12},
  abstract = {Wound infection still remains a significant cause of morbidly and mortality. Hence, studying the spectrum of bacterial etiological agents and their drug susceptibility profile is critical. A prospective study was conducted at Arsho Advanced Medical Laboratory from June 2016 to July 2017. Wound specimens were collected from 366 patients following standard procedures. Specimens were plated and incubated at 37°C for 48 hours. Identification and drug susceptibility testing of cultures were carried out by using the VITEK 2 compact system. Among 366 wound samples cultured, bacteria grew in 271(74%) samples. The highest (81.9%) wound infections were documented among patients with an age group of 15-64 years. Two hundred twenty one bacterial isolates were recovered of which 43.2% were Gram-negative while, 56.8% were Gram-positive. Staphylococcus aureus and Coagulase-Negative Staphylococci were major Gram-positive bacteria while Escherichia coli and Pseudomonas spp.  were the commonest Gram-negative bacteria. Gram-negative bacteria had the highest overall drug resistance rate against ampicillin. Tobramycin and piperacillin/tazobactam combination were effective antimicrobial agents against Gram-negative bacteria. The highest overall resistance rate to Gram-positive bacteria was observed against erythromycin. Vancomycin and linezolid were the most active antimicrobial agents against Gram-positive bacteria. High culture positivity rate of wound infections reported in the present study initiates many similar studies to be conducted on wound in the country. High level of drug resistance to the commonly prescribed drugs dictates a search for better choices.},
 year = {2018}
}

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TY - JOUR
T1 - Spectrum and Drug Susceptibility Profile of Bacteria Recovered from Patients with Wound Infection Referred to Arsho Advanced Medical Laboratory
AU - Adane Bitew
AU - Mesele Admassie
AU - Tigist Getachew
Y1 - 2018/03/07
PY - 2018
N1 - https://doi.org/10.11648/j.cmr.20180701.12
DO - 10.11648/j.cmr.20180701.12
T2 - Clinical Medicine Research
JF - Clinical Medicine Research
JO - Clinical Medicine Research
SP - 8
EP - 17
PB - Science Publishing Group
SN - 2326-9057
UR - https://doi.org/10.11648/j.cmr.20180701.12
AB - Wound infection still remains a significant cause of morbidly and mortality. Hence, studying the spectrum of bacterial etiological agents and their drug susceptibility profile is critical. A prospective study was conducted at Arsho Advanced Medical Laboratory from June 2016 to July 2017. Wound specimens were collected from 366 patients following standard procedures. Specimens were plated and incubated at 37°C for 48 hours. Identification and drug susceptibility testing of cultures were carried out by using the VITEK 2 compact system. Among 366 wound samples cultured, bacteria grew in 271(74%) samples. The highest (81.9%) wound infections were documented among patients with an age group of 15-64 years. Two hundred twenty one bacterial isolates were recovered of which 43.2% were Gram-negative while, 56.8% were Gram-positive. Staphylococcus aureus and Coagulase-Negative Staphylococci were major Gram-positive bacteria while Escherichia coli and Pseudomonas spp. were the commonest Gram-negative bacteria. Gram-negative bacteria had the highest overall drug resistance rate against ampicillin. Tobramycin and piperacillin/tazobactam combination were effective antimicrobial agents against Gram-negative bacteria. The highest overall resistance rate to Gram-positive bacteria was observed against erythromycin. Vancomycin and linezolid were the most active antimicrobial agents against Gram-positive bacteria. High culture positivity rate of wound infections reported in the present study initiates many similar studies to be conducted on wound in the country. High level of drug resistance to the commonly prescribed drugs dictates a search for better choices.
VL - 7
IS - 1
ER -

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Author Information

Adane Bitew

Department of Medical Laboratory Sciences, College of Health Sciences, Addis Ababa University, Addis Ababa, Ethiopia
Mesele Admassie

Arsho Advanced Medical Laboratory Private Limited Company, Addis Ababa, Ethiopia
Tigist Getachew

Department of Medical Laboratory Sciences, College of Health Sciences, Addis Ababa University, Addis Ababa, Ethiopia

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APA Style

Adane Bitew, Mesele Admassie, Tigist Getachew. (2018). Spectrum and Drug Susceptibility Profile of Bacteria Recovered from Patients with Wound Infection Referred to Arsho Advanced Medical Laboratory. Clinical Medicine Research, 7(1), 8-17. https://doi.org/10.11648/j.cmr.20180701.12

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ACS Style

Adane Bitew; Mesele Admassie; Tigist Getachew. Spectrum and Drug Susceptibility Profile of Bacteria Recovered from Patients with Wound Infection Referred to Arsho Advanced Medical Laboratory. Clin. Med. Res. 2018, 7(1), 8-17. doi: 10.11648/j.cmr.20180701.12

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AMA Style

Adane Bitew, Mesele Admassie, Tigist Getachew. Spectrum and Drug Susceptibility Profile of Bacteria Recovered from Patients with Wound Infection Referred to Arsho Advanced Medical Laboratory. Clin Med Res. 2018;7(1):8-17. doi: 10.11648/j.cmr.20180701.12

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@article{10.11648/j.cmr.20180701.12,
  author = {Adane Bitew and Mesele Admassie and Tigist Getachew},
  title = {Spectrum and Drug Susceptibility Profile of Bacteria Recovered from Patients with Wound Infection Referred to Arsho Advanced Medical Laboratory},
  journal = {Clinical Medicine Research},
  volume = {7},
  number = {1},
  pages = {8-17},
  doi = {10.11648/j.cmr.20180701.12},
  url = {https://doi.org/10.11648/j.cmr.20180701.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.cmr.20180701.12},
  abstract = {Wound infection still remains a significant cause of morbidly and mortality. Hence, studying the spectrum of bacterial etiological agents and their drug susceptibility profile is critical. A prospective study was conducted at Arsho Advanced Medical Laboratory from June 2016 to July 2017. Wound specimens were collected from 366 patients following standard procedures. Specimens were plated and incubated at 37°C for 48 hours. Identification and drug susceptibility testing of cultures were carried out by using the VITEK 2 compact system. Among 366 wound samples cultured, bacteria grew in 271(74%) samples. The highest (81.9%) wound infections were documented among patients with an age group of 15-64 years. Two hundred twenty one bacterial isolates were recovered of which 43.2% were Gram-negative while, 56.8% were Gram-positive. Staphylococcus aureus and Coagulase-Negative Staphylococci were major Gram-positive bacteria while Escherichia coli and Pseudomonas spp.  were the commonest Gram-negative bacteria. Gram-negative bacteria had the highest overall drug resistance rate against ampicillin. Tobramycin and piperacillin/tazobactam combination were effective antimicrobial agents against Gram-negative bacteria. The highest overall resistance rate to Gram-positive bacteria was observed against erythromycin. Vancomycin and linezolid were the most active antimicrobial agents against Gram-positive bacteria. High culture positivity rate of wound infections reported in the present study initiates many similar studies to be conducted on wound in the country. High level of drug resistance to the commonly prescribed drugs dictates a search for better choices.},
 year = {2018}
}

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