Apitherapy of Septic Metacarpal and Metatarsal Wounds (An Experimental Study on Donkeys)
Clinical Medicine Research
Volume 8, Issue 4, July 2019, Pages: 77-84
Received: Jul. 26, 2019;
Accepted: Aug. 22, 2019;
Published: Sep. 10, 2019
Views 110 Downloads 14
Sayed Mohammed Hamouda, Department of Microbiology, Animal Health Research Institute, Agriculture Research Center, Assiut, Egypt
Heba Mohammed Mohammed Kuraa, Vet. Surgery, General Organization of Vet. Med, Assiut Directory, Egypt
Mahmoud Abed Zaher Abd El-Samieh, Department of Pathology, Faculty of Veterinary Medicine, Assiut University, Assiut, Egypt
Magda Mahmoud Ali, Department of Surgery, Anesthesiology and Radiology, Faculty of Veterinary Medicine, Assiut University, Assiut, Egypt
Haroun Ali Youssef, Department of Surgery, Anesthesiology and Radiology, Faculty of Veterinary Medicine, Assiut University, Assiut, Egypt
Non-healed wounds are a significant problem in the healthcare system of the distal portions of the limbs. Nine types of honey were tested for wound healing judged by clinical gross healing parameters, rate of wound contraction and sings of epithelization histopathologically. Rounded full thickness skin wounds (1.5cm diameter) were performed at the distal portion of limbs. The wounds were infected by standard dose of MRSA (methicillin resistance Staph. aureus) for 72 hours. The tested types of honey were tested for MIC & MBC against clinical MRSA strain. Different types of honey were applied unprocessed as daily topical wound dressing for the infected wounds. Propolis with black seed honey followed by Hadramout sidr honey, then Egyptian sidr honey proved to have the most antimicrobial activity. Clinical healing appearance revealed that propolis with black seed honey, Egyptian sidr honey and Hadramout sidr honey showed the most preferable clinical parameters (gross appearance and rate of wound contraction), and histopathologically achieved the best healing epithelization 28 days after treatment. The study concluded that among different nine honey types retailed in Egyptian markets, all tested honey types had wound healing activities but with different potencies. Propolis with black seed, Egyptian sidr honey and Hadramout honey were the most potent with highly significance differences (P < 0.01), since achieving the most wound contraction rates, rapid epithelization post 28 days rather than the least MICs & MBCs values. While the marjoram and clover honey were the least effective.
Sayed Mohammed Hamouda,
Heba Mohammed Mohammed Kuraa,
Mahmoud Abed Zaher Abd El-Samieh,
Magda Mahmoud Ali,
Haroun Ali Youssef,
Apitherapy of Septic Metacarpal and Metatarsal Wounds (An Experimental Study on Donkeys), Clinical Medicine Research.
Vol. 8, No. 4,
2019, pp. 77-84.
Molan, P. C. Potential of Honey in the Treatment of Wounds and Burns." Am. J. Clin. Dermatol. 2001; 2 (1): 13-19.
Jull AB, Cullum N, and Dumville JC. Honey as a topical treatment for wounds. Copyright © 2015 The Cochrane Collaboration. Published by John Wiley & Sons, Ltd.
Hussain MB. Role of Honey in Topical and Systemic Bacterial Infections. J. Altern Complement Med 2018 Jan; 24 (1): 15-24. doi: 10.1089/acm.2017.0017.
Ali MA, Saleh AS, Abdul-Hafeez MM and Koreim AM. Honey and medicinal plants in the management of certain surgical bovine clow affections. International Journal of Complementary & Alternative Medicine 2017; 9 (3): 00300.
Abdul-Hafeez MM. Testimony for veterinary apitherapy. Int. J Complement Alt Med; 2019; 2 (1): 15‒22.
Giusto G, Vercelli C, Comino F, et al. A new, easy-to-make pectin-honey hydrogel enhances wound healing in rats. BMC Complementary and Alternative Medicine 2017; 17: 266
Giusto G, Beretta G, Vercelli C, et al. Pectin-honey hydrogel: Characterization, antimicrobial activity and biocompatibility. Biomed Mater Eng. 2018; 29 (3): 347-356. doi: 10.3233/BME-181730.
El-Kased Reham F, Reham IA, Dalia A, and Elmazar MM. Honey-based hydrogel: In vitro and comparative In vivo evaluation for burn wound healing. Scientific Reports 2017; 7: 9692 | DOI: 10.1038/s41598-017-08771-8.
Schell A, Jonathan C, Kath MB and Robert Wetzel. Honey-Based Salve and Burdock Leaf dressings as an alternative to surgical debridement of a traumatic wound eschar. Advances in Wound Care 2019; volume 8, number, 3, 101- 107.
Gomez JH, Schumacher J, Lauten SD, et al. Effects of 3 biologic dressings on healing of cutaneous wounds on the limbs of horses. The Canadian J. of Vet Rese 2004; 68: 49–55.
Theoret CL, Barber SM, Gordon JR. Temporal localization of immunoreactive transforming growth factor beta1 in normal equine skin and in full-thickness dermal wounds. Vet. Surg. 2002; 31: 274–280.
Theoret CL. The pathophysiology of wound repair. Vet Clin North Am Equine Pract 2005; 21: 1–13.
Governa P, Carullo G, Biagi M, et al. Evaluation of the in Vitro wound-healing activity of Calabrian honeys. Antioxidants 2019; 8: 36; doi: 10.3390.
Santos AMN, Ana Paula Duarte Moreira APD, Carvalho C WP, et al. Physically cross-linked gels of PVA with natural polymers as matrices for Manuka honey release in wound-care application. Materials 2019; 12, 559.
Al-Waili Noori. Mixing two different propolis samples potentiates their antimicrobial activity and wound healing property: A novel approach in wound healing and infection. Veterinary World. 2018; Vol. 11, 1188-1195.
Zeleníková, R, Vyhlídalová D. Applying honey dressings to non-healing wounds in elderly persons receiving home care. J. Tissue Viability. 2019 Apr 13. pii: S0965-206X (18)30137-2. doi: 10.1016/j.jtv.2019.04.002.
Quinn PJ, Carter ME, Markey B, et al. Bacteriology: Clinical veterinary microbiology. 6th Ed. Mosby Edinburgh London New York: Oxord Philadelphia St Louis Sydney Toronto; 2011.
Bancroft JD and Stevens A. Theory and practice of Histologic techniques. 3rd Ed. Long Man Group Limited; 1993.
Rosseto HC, Toledo LAS, Francisco LMB. Nanostructured lipid systems modified with waste material of propolis for wound healing: Design, in vitro and in vivo evaluation. Colloids Surf B Biointerfaces 2017; Oct 1; 158: 441-452.
Krupp T, Dos Santos BD, Gama LA, et al. Natural rubber - propolis membrane improves wound healing in second-degree burning model. Int. J Biol. Macromol 2019; 15; 131: 980-988.
Takzaree N, Hadjiakhondi A, Hassanzadeh G, et al. Synergistic effect of honey and propolis on cutaneous wound healing in rats. Acta Medica Iranica 2016; Vol. 54, No. 4: 233-239.
Sharaf S and El-Naggar ME. Wound dressing properties of cationized cotton fabric treated with carrageenan/cyclodextrin hydrogel loaded with honey bee propolis extract. Int J Biol Macromol 2019; Apr 18; 133: 583-591.
23-Gül A and Pehlivan T. Antioxidant activities of some monofloral honey types produced across Turkey. Saudi Journal of Biological Sciences 2018; 25: 1056–1065
Firdose A, Nisar A, Dsouza MR. Evaluation of in vitro antimicrobial activity of Indian honey on burn wound isolates. J. of Chemical and Pharma Research. 2016; 8 (3): 1027–1034.
Patel A, Chauhan PB. Antimicrobial effect of honey on MRSA isolated from pus samples. Int J Drug Res Tech. 2016; 6 (2): 58–63.
Almasaudi SB, Al–Nahari AAM, Abd El–Ghany ESM, et al. Antimicrobial effect of different types of honey on Staphylococcus aureus. Saudi J Biol Sci. 2017; 24 (6): 1255–1261.
Poovelikunnel TT, Gethin G, Solanki D, et al. Randomized controlled trial of honey versus mupirocin to decolonize patients with nasal colonization of meticillin–resistant Staphylococcus aureus. J. Hosp Infect. 2017; 98 (2): 141–148.
Hamouda SM, Abd El Rahman MF, Abdul-Hafeez MM and Gerges AE. Egyptian fennel honey and/or propolis against MRSA harboring both mecA & icaA genes. Int. J. Complement Alt Med. 2018; 11 (3): 180‒185.
Benjamin A, Minden-Birkenmaier and Gary L. Bowlin. Honey-based templates in wound healing and tissue engineering. Bioengineering; 2018, 5, 46.
Abdul-Hafeez MM. Testimony for veterinary apitherapy. Int. J. Complement. Alt. Med. 2019; 12 (1): 15‒22.
Mama M, Teshome T, Detamo J. Antibacterial activity of honey against methicillin-resistant Staphylococcus aureus: A laboratory-based experimental study. Int J. Microbiol. 2019; Apr 3; 2019: 7686130. doi: 10.1155/2019/7686130.
Raghukumar R, Vali L, Watson D, Fearnley J and Seidel V. Antimethicillin-resistant Staphylococcus aureus (MRSA) activity of 'pacific propolis' and isolated prenylflavanones. Phytother Rese 2010; 24 (8): 1181-1187.
Bryan J, Redden P and Traba C. The mechanism of action of Russian propolis ethanol extracts against two antibiotic-resistant biofilm-forming bacteria. Lett. Appl. Microbiol (2016); 62 (2): 192-198.
Al–Ani I, Zimmermann S, Reichling J, et al. Antimicrobial activities of European propolis collected from various geographic origins alone and in combination with antibiotics. Medicines 2018; 5 (1): 2.
Al–Waili N, Al–Ghamdi A, Ansari MJ, et al. Synergistic effects of honey and propolis toward drug multi–resistant Staphylococcus aureus, Escherichia coli and Candida albicans isolates in single and polymicrobial cultures. Int J Med Sci. 2012; 9 (9): 793–800.
Živković L, Bajić V, Dekanski D, et al. Manuka honey attenuates oxidative damage induced by H2O2 in human whole blood in vitro. Food Chem Toxicol. 2018; 119: 61–65.
Mani R, Natesan V. Chrysin: Sources, beneficial pharmacological activities, and molecular mechanism of action. Phytochemistry 2018; 145: 187–196.
Cianciosi D, Forbes–Hernández TY, Afrin S, et al. Phenolic compounds in honey and their associated health benefits: A Review. Molecules. 2018; 23 (9): pii: E2322.
Martinotti S, Laforenza U, Patrone M, Francesco Moccia F, and Ranzato E. Honey-mediated wound healing: h2o2 entry through AQP3 determines extracellular Ca2+ influx. Int. J. Mol. Sci. 2019, 20, 764; doi: 10.3390/ijms 20030764.
Georgieva K, Milena Popova M, Dimitrova L, et al. Phytochemical analysis of Vietnamese propolis produced by the stingless bee Lisotrigona cacciae. journal. pone. 2019; 24, https://doi.org/10.1371
Ghaderi R, Afshar M, Akhbarie H, and Golalipour MJ. Comparison of the efficacy of honey and animal oil in accelerating healing of full thickness wound of mice skin. Int. J. Morphol., 2010; 28 (1): 193-198.
Imtara H, Al-Waili N, Bakour M, Al-Waili W, and Badiaa Lyoussi B. Evaluation of antioxidant, diuretic, and wound healing effect of Tulkarm honey and its effect on kidney function in rats. Veterinary world.org/Vol.11/October-2018/21.
Haryanto RN MS, Tamae Urai RN MS, Kanae Mukai RN, MS, et al. Effectiveness of Indonesian honey on the acceleration of cutaneous wound healing: An experimental study in mice. Wounds 2012; 24 (4): 110–119.
Kotian S, Bhat K, Pai S, et al. The role of natural medicines on wound healing: a biomechanical, histological, biochemical and molecular study. Ethiop J. Health Sci. 2018; Vol. 28, No. 6.
Aderounmu AO, Omonisi AE, Akingbasote JA, et al. Wound-healing and potential anti-keloidal properties of the latex of calotropis procera (aiton) asclepiadaceae in rabbits. Afr J Tradit Complement Altern Med. 2013; 10 (3): 574-579.
Zohdi RM, Zuki ABZ, and Norimah Y, et al. Gelam (Melaleuca spp.) honey-based hydrogel as burn wound dressing. Evidence-Based Complementary and Alternative Medicine Volume 2012, Article ID 843025, 7 pages.
Abdel-Wahed RE, El-Kammar MH, Korittum AS and Edrees IR. Surgical and histological evaluation of the effectiveness of propolis on wound healing. Alexandria Journal of Veterinary Sciences 2013; 39: 52-63.
Rufatto LC, Denis Amilton dos Santos, Fl´avio Marinho. Red propolis: Chemical composition and pharmacological activity. Asian Pac. J. Trop. Biomed.; 2017; 7 (7): 591–598.
Picolotto A, Pergher D, Guilherme Pacheco Pereira, et al. Bacterial cellulose membrane associated with red propolis as phytomodulator: Improved healing effects in experimental models of diabetes mellitus. Biomedicine & Pharmacotherapy 2019; 112; 108640.
Gupta SS, Singh O, Bhagel PS, et al. Honey dressing versus Silver Sulfadiazene dressing for wound healing in burn patients: A retrospective study. Journal of Cutaneous and Aesthetic Surgery 2018; Volume 4, Issue 3.
Pasupuleti VR, Sammugam L, Ramesh N, Siew Hua Gan SH. Honey, Propolis, and Royal Jelly: A comprehensive review of their biological actions and health benefits. Oxidative Medicine and Cellular Longevity, Volume 2017, Article ID 1259510, 21 pages.
Weiß KT, Fante M, Köhl G, et al. Proton-sensing G protein-coupled receptors as regulators of cell proliferation and migration during tumor growth and wound healing. Experimental Dermatology 2017; Volume 26 Issue 2, 127 -132.
Majtan J (2014): Honey: An immunomodulator in wound healing. Wound Rep Reg 2014; 22 187–192.
Majtan J, Jana Bohova, Rocio Garcia-Villalba, et al. Fir honeydew honey flavonoids inhibit TNF-a-induced MMP-9 expression in human keratinocytes: a new action of honey in wound healing. Arch. Dermatol. Res. 2013; 305: 619–627.
Martinotti S, Bucekova M, Majtan J, Ranzato E. Honey: An effective regenerative medicine product in wound management. Curr. Med. Chem. 2018; May 10.
Alizadeh AM, Sohanaki H, Mahmod Khaniki M, et al. The effect of Teucrium Polium honey on the wound healing and tensile strength in rat. Iranian Journal of Basic Medical Sciences 2011; Vol. 14, No. 6: 499-505.
Elkhenany Hoda, Nagwa El-Badri, Madhu Dhar. Green propolis extract promotes in vitro proliferation, differentiation, and migration of bone marrow stromal cells. Biomedicine & Pharmacotherapy 2019; 115 108861.
Takzaree Nasrin, Hassanzadeh G, Rouini MR, et al. Evaluation of the effects of local application of thyme honey in open cutaneous wound healing. Iran J Public Health 2017; Vol. 46, No. 4: 545-551.
Anjum SI, Ullah A, Khalid Ali Khan KA, et al. Composition and functional properties of propolis (bee glue): A review. Saudi Journal of Biological Sciences 2018; 08.013, doi.org/10.1016/j.sjbs.
Albuquerque-Júnior RLC, Barreto ALS, Pires AJ, Reis FP, Lima SO, et al. Effect of bovine type-I collagen-based films containing red propolis on dermal wound healing in rodent model. Int J Morphol 2009; 27: 1105-1110.
Almeida EB, Cardoso JC, Lima AK, Oliveira NL, et al. The incorporation of Brazilian propolis into collagen-based dressing films improves dermal burn healing. J Ethnopharmacol 2013; 147: 419-25.
Ranzato E, Martinotti S, Burlando B. Epithelial mesenchymal transition traits in honey-driven keratinocyte wound healing: Comparison among different honeys. Wound Repair Regener. 2012; 20, 778–785.