Grape Seed Proanthocyanidin Extract Down-Regulates Autophagic Proteins (LC3II and Beclin-1) in a Mouse Model of Neonatal Hypoxic-Ischemic Brain Injury
Clinical Medicine Research
Volume 8, Issue 1, January 2019, Pages: 16-20
Received: Jan. 31, 2019; Accepted: Mar. 11, 2019; Published: Mar. 25, 2019
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Authors
Li Luo, School of Biosciences & Biopharmaceutics, Guangdong Pharmaceutical University, Guangzhou, People's Republic of China
Xing Tu, School of Biosciences & Biopharmaceutics, Guangdong Pharmaceutical University, Guangzhou, People's Republic of China
Wei Liu, School of Clinical Medicine, the First Affiliated Hospital of Guangdong Pharmaceutical University, Guangzhou, People's Republic of China
Xuexia Zhang, Department of Anesthesiology, the Fifth Affiliated Hospital, Sun Yat-Sen University, Zhuhai, People's Republic of China
Yilin Liu, School of Clinical Medicine, the First Affiliated Hospital of Guangdong Pharmaceutical University, Guangzhou, People's Republic of China
Wenyan Zhao, School of Clinical Medicine, the First Affiliated Hospital of Guangdong Pharmaceutical University, Guangzhou, People's Republic of China
Junhua Yang, School of Biosciences & Biopharmaceutics, Guangdong Pharmaceutical University, Guangzhou, People's Republic of China
Mengxia Wang, Intensive Care Unit, Guangdong No. 2 Provincial People's Hospital, Guangzhou, People's Republic of China
Jing Liu, School of Biosciences & Biopharmaceutics, Guangdong Pharmaceutical University, Guangzhou, People's Republic of China
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Abstract
Neonatal hypoxic-ischemic encephalopathy (HIE) is a severe brain disease that often leads to death or irreversible sequelae. The aim of this study was to determine the effect of a naturally active drug (grape seed proanthocyanidin extract [GSPE]) on the expression of autophagic proteins in a mouse model of neonatal hypoxic-ischemic brain injury. In this study immunofluorescence staining and Western blotting were used to detect the expression of autophagy markers (LC3II and beclin1) in the brains of neonatal mice with hypoxic-ischemic brain injuries after GSPE administration. Our study showed that GSPE pre-treatment down-regulates LC3II and beclin-1 expression, thus GSPE may be a potential drug for the treatment of HIE.
Keywords
Grape Seed Proanthocyanidins Extract, Hypoxic-Ischemic Brain Injury, Autophagy
To cite this article
Li Luo, Xing Tu, Wei Liu, Xuexia Zhang, Yilin Liu, Wenyan Zhao, Junhua Yang, Mengxia Wang, Jing Liu, Grape Seed Proanthocyanidin Extract Down-Regulates Autophagic Proteins (LC3II and Beclin-1) in a Mouse Model of Neonatal Hypoxic-Ischemic Brain Injury, Clinical Medicine Research. Vol. 8, No. 1, 2019, pp. 16-20. doi: 10.11648/j.cmr.20190801.13
Copyright
Copyright © 2019 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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