Clinical Medicine Research

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Carboxymethyllysine, an Advanced Glycation End-Product, Promotes the Invasion and Migration of Lung Cancer A549 Cells

Received: 08 August 2017    Accepted: 07 September 2017    Published: 16 October 2017
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Abstract

Effects of carboxymethyllysine (CML), an advanced glycation end-product (AGE), at 1, 2, 4, 8 or 16 μmol/l upon invasion and migration of A549 cells, non-small cell lung cancer (NSCLC) cells, were investigated. Results showed that CML at used test doses did not affect A549 cell growth. However, CML at 4-16 μmol/l enhanced both invasion and migration, and stimulated the release of reactive oxygen species, interleukin-6 and tumor necrosis factor-alpha in A549 cells. CML at 2-16 μmol/l increased protein expression of AGE receptor, p47phox, intercellular adhesion molecule-1, fibronectin, kappa-B (NF- κB) p65 and p-p38 in A549 cells. CML only at 4-16 μmol/l increased matrix metalloproteinase-2 expression in A549 cells. These findings indicated that CML might benefit NSCLC metastasis through promoting invasion and migration.

DOI 10.11648/j.cmr.20170605.11
Published in Clinical Medicine Research (Volume 6, Issue 5, September 2017)
Page(s) 149-156
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Carboxymethyllysine, Advanced Glycation End Products, NSCLC, A549 Cell, Migration, Invasion

References
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Author Information
  • Department of Respiratory Therapy, China Medical University, Taichung City, Taiwan; Department of Internal Medicine, China Medical University Hospital, Taichung City, Taiwan

  • Department of Food Nutrition and Health Biotechnology, Asia University, Taichung City, Taiwan

  • Department of Food Nutrition and Health Biotechnology, Asia University, Taichung City, Taiwan

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  • APA Style

    Te-chun Hsia, Mei-chin Yin, Zhi-hong Wang. (2017). Carboxymethyllysine, an Advanced Glycation End-Product, Promotes the Invasion and Migration of Lung Cancer A549 Cells. Clinical Medicine Research, 6(5), 149-156. https://doi.org/10.11648/j.cmr.20170605.11

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    ACS Style

    Te-chun Hsia; Mei-chin Yin; Zhi-hong Wang. Carboxymethyllysine, an Advanced Glycation End-Product, Promotes the Invasion and Migration of Lung Cancer A549 Cells. Clin. Med. Res. 2017, 6(5), 149-156. doi: 10.11648/j.cmr.20170605.11

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    AMA Style

    Te-chun Hsia, Mei-chin Yin, Zhi-hong Wang. Carboxymethyllysine, an Advanced Glycation End-Product, Promotes the Invasion and Migration of Lung Cancer A549 Cells. Clin Med Res. 2017;6(5):149-156. doi: 10.11648/j.cmr.20170605.11

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  • @article{10.11648/j.cmr.20170605.11,
      author = {Te-chun Hsia and Mei-chin Yin and Zhi-hong Wang},
      title = {Carboxymethyllysine, an Advanced Glycation End-Product, Promotes the Invasion and Migration of Lung Cancer A549 Cells},
      journal = {Clinical Medicine Research},
      volume = {6},
      number = {5},
      pages = {149-156},
      doi = {10.11648/j.cmr.20170605.11},
      url = {https://doi.org/10.11648/j.cmr.20170605.11},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.cmr.20170605.11},
      abstract = {Effects of carboxymethyllysine (CML), an advanced glycation end-product (AGE), at 1, 2, 4, 8 or 16 μmol/l upon invasion and migration of A549 cells, non-small cell lung cancer (NSCLC) cells, were investigated. Results showed that CML at used test doses did not affect A549 cell growth. However, CML at 4-16 μmol/l enhanced both invasion and migration, and stimulated the release of reactive oxygen species, interleukin-6 and tumor necrosis factor-alpha in A549 cells. CML at 2-16 μmol/l increased protein expression of AGE receptor, p47phox, intercellular adhesion molecule-1, fibronectin, kappa-B (NF- κB) p65 and p-p38 in A549 cells. CML only at 4-16 μmol/l increased matrix metalloproteinase-2 expression in A549 cells. These findings indicated that CML might benefit NSCLC metastasis through promoting invasion and migration.},
     year = {2017}
    }
    

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    T1  - Carboxymethyllysine, an Advanced Glycation End-Product, Promotes the Invasion and Migration of Lung Cancer A549 Cells
    AU  - Te-chun Hsia
    AU  - Mei-chin Yin
    AU  - Zhi-hong Wang
    Y1  - 2017/10/16
    PY  - 2017
    N1  - https://doi.org/10.11648/j.cmr.20170605.11
    DO  - 10.11648/j.cmr.20170605.11
    T2  - Clinical Medicine Research
    JF  - Clinical Medicine Research
    JO  - Clinical Medicine Research
    SP  - 149
    EP  - 156
    PB  - Science Publishing Group
    SN  - 2326-9057
    UR  - https://doi.org/10.11648/j.cmr.20170605.11
    AB  - Effects of carboxymethyllysine (CML), an advanced glycation end-product (AGE), at 1, 2, 4, 8 or 16 μmol/l upon invasion and migration of A549 cells, non-small cell lung cancer (NSCLC) cells, were investigated. Results showed that CML at used test doses did not affect A549 cell growth. However, CML at 4-16 μmol/l enhanced both invasion and migration, and stimulated the release of reactive oxygen species, interleukin-6 and tumor necrosis factor-alpha in A549 cells. CML at 2-16 μmol/l increased protein expression of AGE receptor, p47phox, intercellular adhesion molecule-1, fibronectin, kappa-B (NF- κB) p65 and p-p38 in A549 cells. CML only at 4-16 μmol/l increased matrix metalloproteinase-2 expression in A549 cells. These findings indicated that CML might benefit NSCLC metastasis through promoting invasion and migration.
    VL  - 6
    IS  - 5
    ER  - 

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