Hydroxyapatite and Demineralized Bone Matrix from Marine Food Waste – A Possible Bone Implant

Rethinam Senthil; Sathyaraj Weslen Vedakumari; Thotapalli Parvathaleswara Sastry

doi:doi:10.11648/j.ajmsp.20180301.11

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Hydroxyapatite and Demineralized Bone Matrix from Marine Food Waste – A Possible Bone Implant

Rethinam Senthil, Sathyaraj Weslen Vedakumari, Thotapalli Parvathaleswara Sastry

Published in American Journal of Materials Synthesis and Processing (Volume 3, Issue 1)

Received: 28 September 2017 Accepted: 18 October 2017 Published: 18 January 2018

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Abstract

In the present study, a novel bone implant (BI) was prepared using demineralized bone matrix (DBM) and hydroxyapatite (HA) isolated from Bluefin trevally (BT) bones, which was considered to be a marine industry food waste. Gelatin (GA) was used as a binder. Physico-chemical characterization and in vitro studies were carried out using this implant. Fourier transform infrared spectrum of BI exhibited the characteristic bands of all the three components viz., DBM, HA and GA, while scanning electron microscopic studies revealed the irregular shape of the particles. The mechanical properties of BI were also appreciable. In vitro studies were carried out using Human keratinocyte cell line (HaCaT), wherein MTT (3-(4,5-dimethylazol-2-yl)-2,5-diphenyl-tetrazolium bromide) assay proved the biocompatibility of BI. From the results obtained it could be stated that BI prepared from waste marine bones could serve as a promising biomaterial for bone tissue engineering applications.

Published in	American Journal of Materials Synthesis and Processing (Volume 3, Issue 1)
DOI	10.11648/j.ajmsp.20180301.11
Page(s)	1-6
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Fish Waste, Recycling, Bone Implant, Biomaterial

References

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APA Style

Rethinam Senthil, Sathyaraj Weslen Vedakumari, Thotapalli Parvathaleswara Sastry. (2018). Hydroxyapatite and Demineralized Bone Matrix from Marine Food Waste – A Possible Bone Implant. American Journal of Materials Synthesis and Processing, 3(1), 1-6. https://doi.org/10.11648/j.ajmsp.20180301.11

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ACS Style

Rethinam Senthil; Sathyaraj Weslen Vedakumari; Thotapalli Parvathaleswara Sastry. Hydroxyapatite and Demineralized Bone Matrix from Marine Food Waste – A Possible Bone Implant. Am. J. Mater. Synth. Process. 2018, 3(1), 1-6. doi: 10.11648/j.ajmsp.20180301.11

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AMA Style

Rethinam Senthil, Sathyaraj Weslen Vedakumari, Thotapalli Parvathaleswara Sastry. Hydroxyapatite and Demineralized Bone Matrix from Marine Food Waste – A Possible Bone Implant. Am J Mater Synth Process. 2018;3(1):1-6. doi: 10.11648/j.ajmsp.20180301.11

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@article{10.11648/j.ajmsp.20180301.11,
  author = {Rethinam Senthil and Sathyaraj Weslen Vedakumari and Thotapalli Parvathaleswara Sastry},
  title = {Hydroxyapatite and Demineralized Bone Matrix from Marine Food Waste – A Possible Bone Implant},
  journal = {American Journal of Materials Synthesis and Processing},
  volume = {3},
  number = {1},
  pages = {1-6},
  doi = {10.11648/j.ajmsp.20180301.11},
  url = {https://doi.org/10.11648/j.ajmsp.20180301.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajmsp.20180301.11},
  abstract = {In the present study, a novel bone implant (BI) was prepared using demineralized bone matrix (DBM) and hydroxyapatite (HA) isolated from Bluefin trevally (BT) bones, which was considered to be a marine industry food waste. Gelatin (GA) was used as a binder. Physico-chemical characterization and in vitro studies were carried out using this implant. Fourier transform infrared spectrum of BI exhibited the characteristic bands of all the three components viz., DBM, HA and GA, while scanning electron microscopic studies revealed the irregular shape of the particles. The mechanical properties of BI were also appreciable. In vitro studies were carried out using Human keratinocyte cell line (HaCaT), wherein MTT (3-(4,5-dimethylazol-2-yl)-2,5-diphenyl-tetrazolium bromide) assay proved the biocompatibility of BI. From the results obtained it could be stated that BI prepared from waste marine bones could serve as a promising biomaterial for bone tissue engineering applications.},
 year = {2018}
}

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TY  - JOUR
T1  - Hydroxyapatite and Demineralized Bone Matrix from Marine Food Waste – A Possible Bone Implant
AU  - Rethinam Senthil
AU  - Sathyaraj Weslen Vedakumari
AU  - Thotapalli Parvathaleswara Sastry
Y1  - 2018/01/18
PY  - 2018
N1  - https://doi.org/10.11648/j.ajmsp.20180301.11
DO  - 10.11648/j.ajmsp.20180301.11
T2  - American Journal of Materials Synthesis and Processing
JF  - American Journal of Materials Synthesis and Processing
JO  - American Journal of Materials Synthesis and Processing
SP  - 1
EP  - 6
PB  - Science Publishing Group
SN  - 2575-1530
UR  - https://doi.org/10.11648/j.ajmsp.20180301.11
AB  - In the present study, a novel bone implant (BI) was prepared using demineralized bone matrix (DBM) and hydroxyapatite (HA) isolated from Bluefin trevally (BT) bones, which was considered to be a marine industry food waste. Gelatin (GA) was used as a binder. Physico-chemical characterization and in vitro studies were carried out using this implant. Fourier transform infrared spectrum of BI exhibited the characteristic bands of all the three components viz., DBM, HA and GA, while scanning electron microscopic studies revealed the irregular shape of the particles. The mechanical properties of BI were also appreciable. In vitro studies were carried out using Human keratinocyte cell line (HaCaT), wherein MTT (3-(4,5-dimethylazol-2-yl)-2,5-diphenyl-tetrazolium bromide) assay proved the biocompatibility of BI. From the results obtained it could be stated that BI prepared from waste marine bones could serve as a promising biomaterial for bone tissue engineering applications.
VL  - 3
IS  - 1
ER  -

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Author Information

Rethinam Senthil

Biological Materials Lab, Central Leather Research Institute, Chennai, India
Sathyaraj Weslen Vedakumari

Faculty of Allied Health Science, Chettinad Academy of Research & Education, Chennai, India
Thotapalli Parvathaleswara Sastry

Biological Materials Lab, Central Leather Research Institute, Chennai, India

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APA Style

Rethinam Senthil, Sathyaraj Weslen Vedakumari, Thotapalli Parvathaleswara Sastry. (2018). Hydroxyapatite and Demineralized Bone Matrix from Marine Food Waste – A Possible Bone Implant. American Journal of Materials Synthesis and Processing, 3(1), 1-6. https://doi.org/10.11648/j.ajmsp.20180301.11

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ACS Style

Rethinam Senthil; Sathyaraj Weslen Vedakumari; Thotapalli Parvathaleswara Sastry. Hydroxyapatite and Demineralized Bone Matrix from Marine Food Waste – A Possible Bone Implant. Am. J. Mater. Synth. Process. 2018, 3(1), 1-6. doi: 10.11648/j.ajmsp.20180301.11

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AMA Style

Rethinam Senthil, Sathyaraj Weslen Vedakumari, Thotapalli Parvathaleswara Sastry. Hydroxyapatite and Demineralized Bone Matrix from Marine Food Waste – A Possible Bone Implant. Am J Mater Synth Process. 2018;3(1):1-6. doi: 10.11648/j.ajmsp.20180301.11

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@article{10.11648/j.ajmsp.20180301.11,
  author = {Rethinam Senthil and Sathyaraj Weslen Vedakumari and Thotapalli Parvathaleswara Sastry},
  title = {Hydroxyapatite and Demineralized Bone Matrix from Marine Food Waste – A Possible Bone Implant},
  journal = {American Journal of Materials Synthesis and Processing},
  volume = {3},
  number = {1},
  pages = {1-6},
  doi = {10.11648/j.ajmsp.20180301.11},
  url = {https://doi.org/10.11648/j.ajmsp.20180301.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajmsp.20180301.11},
  abstract = {In the present study, a novel bone implant (BI) was prepared using demineralized bone matrix (DBM) and hydroxyapatite (HA) isolated from Bluefin trevally (BT) bones, which was considered to be a marine industry food waste. Gelatin (GA) was used as a binder. Physico-chemical characterization and in vitro studies were carried out using this implant. Fourier transform infrared spectrum of BI exhibited the characteristic bands of all the three components viz., DBM, HA and GA, while scanning electron microscopic studies revealed the irregular shape of the particles. The mechanical properties of BI were also appreciable. In vitro studies were carried out using Human keratinocyte cell line (HaCaT), wherein MTT (3-(4,5-dimethylazol-2-yl)-2,5-diphenyl-tetrazolium bromide) assay proved the biocompatibility of BI. From the results obtained it could be stated that BI prepared from waste marine bones could serve as a promising biomaterial for bone tissue engineering applications.},
 year = {2018}
}

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TY  - JOUR
T1  - Hydroxyapatite and Demineralized Bone Matrix from Marine Food Waste – A Possible Bone Implant
AU  - Rethinam Senthil
AU  - Sathyaraj Weslen Vedakumari
AU  - Thotapalli Parvathaleswara Sastry
Y1  - 2018/01/18
PY  - 2018
N1  - https://doi.org/10.11648/j.ajmsp.20180301.11
DO  - 10.11648/j.ajmsp.20180301.11
T2  - American Journal of Materials Synthesis and Processing
JF  - American Journal of Materials Synthesis and Processing
JO  - American Journal of Materials Synthesis and Processing
SP  - 1
EP  - 6
PB  - Science Publishing Group
SN  - 2575-1530
UR  - https://doi.org/10.11648/j.ajmsp.20180301.11
AB  - In the present study, a novel bone implant (BI) was prepared using demineralized bone matrix (DBM) and hydroxyapatite (HA) isolated from Bluefin trevally (BT) bones, which was considered to be a marine industry food waste. Gelatin (GA) was used as a binder. Physico-chemical characterization and in vitro studies were carried out using this implant. Fourier transform infrared spectrum of BI exhibited the characteristic bands of all the three components viz., DBM, HA and GA, while scanning electron microscopic studies revealed the irregular shape of the particles. The mechanical properties of BI were also appreciable. In vitro studies were carried out using Human keratinocyte cell line (HaCaT), wherein MTT (3-(4,5-dimethylazol-2-yl)-2,5-diphenyl-tetrazolium bromide) assay proved the biocompatibility of BI. From the results obtained it could be stated that BI prepared from waste marine bones could serve as a promising biomaterial for bone tissue engineering applications.
VL  - 3
IS  - 1
ER  -

Copy | Download