International Journal of Materials Science and Applications

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Can Anodic Aluminium Oxide Nanomembranes Treated with Nanometre Scale Hydroxyapatite be Used as a Cell Culture Substrate

Received: 28 August 2014    Accepted: 11 September 2014    Published: 30 October 2014
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Abstract

In this study we investigate the biomedical potential of composite membranes composed of anodic aluminium oxide (AAO) and nanometre scale hydroxyapatite (HAP). The nano-porous AAO membranes were produced using a temperature controlled two-step anodization technique. The AAO/HAP composite membranes were formed using the solution template wetting technique. The Cercopithecus aethiops (African green monkey) Kidney (Vero) epithelial cell line was used to demonstrate the biocompatibility of the synthesised membranes and composites. Investigating cell adhesion, morphology and proliferation over a 72 h period assessed cellular interactions and responses of the cell line to the various membranes types.

DOI 10.11648/j.ijmsa.20140306.19
Published in International Journal of Materials Science and Applications (Volume 3, Issue 6, November 2014)
Page(s) 331-338
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Biomaterials, Anodic Aluminium Oxide, Bioceramics, Regenerative Medicine

References
[1] G.E.J. Poinern, R. Shackleton, S.I. Mamun and D. Fawcett, Significance of novel bioinorganic anodic aluminium oxide nanoscaffolds for promoting cellular response. Nanotechnology, Science and Applications, vol. 3, pp. 1-14, 2010.
[2] A.P. Li, F. Muller, A. Birner, K. Nielsch, U. Gosele, Hexagonal pore arrays with a 50–420 inter-pore distance formed by self-organization in anodic alumina. J. Appl. Phys., vol. 84, pp. 6023-6026, 1998.
[3] J.P. O’Sullivan, G.C. Wood, Nucleation and growth of porous anodic films on aluminium. P. Roy. Lond. A. Mat, vol. A317, pp. 511-543, 1970.
[4] H. Masuda, K. Yada, A. Osaka, Self-ordering of cell configuration of anodic porous alumina with large-size pores in phosphoric acid solution. Jpn. J. Appl. Phys. Vol. 37: pp. L1340-L1342, 1998.
[5] M. Ghorbani, F. Nasirpouri, A. Iraji-zad, A. Saedi, On the growth sequence of highly ordered nano-porous anodic aluminium oxide. Mater. Des. Vol. 27: pp. 983-988, 2006.
[6] D.H. Choi, P.S. Lee, W. Hwang, K.H. Lee, H.C. Park, Measurement of pore sizes for anodic aluminium oxide (AAO). Curr. Appl. Phys, vol. 6S1:pp. e125-e129, 2006.
[7] G.E.J. Poinern, N. Ali, D. Fawcett, Progress in nano-engineered anodic aluminium oxide membrane development. Materials, vol. 4, pp. 487-526, 2011.
[8] H. Masuda, M. Satoh, Fabrication of gold nano-dot array using anodic porous alumina as an evaporation mask. Jpn. J. Appl. Phys, vol. 35: pp. L126-L129, 1996.
[9] S. Shingubara, Fabrication of nanomaterials using porous alumina templates. J. Nanoparticle Res, vol. 5, pp. 17-30, 2003.
[10] X.J. Wu, F. Zhu, C. Mu, Y. Liang, L. Xu, Q. Chen, R. Chen, D. Xu, Electrochemical synthesis and applications of oriented and hierarchically quasi-1D semiconducting nanos tructures. Coord. Chem. Rev. Vol. 254: pp. 1135-1150, 2010.
[11] Y. Kim, B. Jung, H. Lee, H. Kim, K. Lee, H. Park, Capacitive humidity sensor design based on aluminium oxide. Sensor. Actuator B, vol. 141, pp. 441-446, 2009.
[12] R. Bogue, The fabrication and assembly of nano-electronic devices. Assembly Automation, vol. 30, pp. 206-212, 2010.
[13] Martin CR, Membrane based synthesis of nanomaterials, Chem. Mater. Vol. 8, pp. 1739-1746, 1996.
[14] G.E.J. Poinern, D. Fawcett, Y.J. Ng, N. Ali, R.K. Brundavanam, Z.T. Jiang, Nanoengineering a biocompatible inorganic scaffold for skin wound healing. J. BioMed. Nanotech, vol. 6, pp. 497-510, 2010.
[15] D. Bruggemann, Nanoporous aluminium oxide membranes as cell substrates. Journal of Nanomaterials. Article ID 460870, pp. 1-18, 2013.
[16] G.E.J. Poinern, N. Ali, C. Berry, P. Singh, and D. Fawcett, Biocompatibility of synthesis nano-porous anodic aluminium oxide membranes for use as a cell culture substrate for Madin-Darby Canine Kidney Cells: A preliminary study. J. Tissue Sci Eng., vol. 3, No. 119, pp. 1-7, 2012.
[17] L.G. Parkinson, N.L. Giles, K.F. Adcroft, M.W. Fear, F.M. Wood, G.E.J. Poinern, The potential of nanoporous anodic aluminium oxide membranes to influence skin wound repair. Tissue Eng Part A. Vol. 15, No. 12, pp. 3753–3763, 2009.
[18] A. Hoess, N. Teuscher, A. Thormann, H. Aurich, A. Heilmann, Cultivation of hepatoma cell line HepG2 on nanoporous aluminum oxide membranes. Acta Biomater. Vol. 3, No. 1, pp. 43–50, 2007.
[19] D.C. Miller, A. Thapa, K.M. Haberstroh, T.J. Webster, Endothelial and vascular smooth muscle cell function on poly (lactic-co-glycolic acid) with nano-structure features. Biomaterials, vol. 25, No. 1, pp. 53–61, 2004.
[20] M. Karlsson, E. Pålsgård, P.R. Wilshaw, L. di Silvio, Initial in vitro interaction of osteoblasts with nano-porous alumina. Biomaterials, vol. 24, No. 18, pp. 3039–3046, 2003.
[21] P. Roach, D. Eglin, K. Rohde, C. Perry, Modern biomaterials: A review–Bulk properties and implications of surface modifications. J. Mater. Sci. Mater. Med, vol. 18, pp. 1263-1277, 2007.
[22] E. Damien, P.A. Revell, Coralline hydroxyapatite bone substitute: A review of experimental studies and biomedical applications, J. Appl. Biomater. Biomech, vol. 2, pp. 65-73, 2004.
[23] D.W. Hutmacher, J.T. Schantz, C.X.F. Lam, K.C. Tan, T.C. Lim, State of the art and future directions of scaffold-based bone engineering from a biomaterials perspective, J. Tissue Eng. Regen. Med, vol. 1, pp. 245-260, 2007.
[24] W.J.E.M. Habraken, J.G.C. Wolke, J.A. Jansen, Ceramic composites as matrices and scaffolds for drug delivery in tissue engineering, Adv. Drug Deli. Rev., vol. 59, pp. 234-248, 2007.
[25] A. Blom A, Which scaffold for which application, Curr. Orthop, vol. 21, pp. 280-287, 2007.
[26] P. Habibovic, K. de Groot, Osteoinductive biomaterials properties and relevance in bone repair, J. Tissue Eng and Regen. Med, vol. 1, pp. 25-32, 2007.
[27] S.J. Kalita, A. Bhardwaj, H.A. Bhatt, Nanocrystalline calcium phosphate ceramics in biomedical engineering, Mater. Sci. Eng. C, vol. 27, pp. 441-449, 2007.
[28] Virology Laboratory procedure VIW-17, Animal Health Laboratories, Animal Virology, Department of Agriculture and Food, 3 Baron Hay Court, Kensington, Western Australia 6151, Australia.
[29] G.E.J. Poinern, X. Le, M. O’Dea, T. Becker, D. Fawcett, Chemical Synthesis, Characterisation, and Biocompatibility of Nanometre Scale Porous Anodic Aluminium Oxide Membranes for Use as a Cell Culture Substrate for the Vero Cell Line: A Preliminary Study. BioMed Research International. Article ID 238762, pp. 1-10, 2014.
[30] G.E.J. Poinern, X. Le, M. Hager, T. Becker, D. Fawcett, Electrochemical Synthesis, Characterisation, and Preliminary Biological Evaluation of an Anodic Aluminium Oxide Membrane with a pore size of 100 nanometres for a Potential Cell Culture Substrate. American Journal of Biomedical Engineering, vol. 3, No. 6, pp. 119-131, 2013.
[31] C. Schafer, B. Borm, S. Born, C. Mohr, E.M. Eibl, B. Hoffman B, One step ahead: Role of filopodia in adhesion formation during cell migration of Keratinocytes. Experimental Cell Research, vol. 315, pp.1212-1224, 2009.
Author Information
  • Department of Physics, Energy Studies and Nanotechnology School of Engineering and Energy, Murdoch University, Perth, Western Australia, Australia

  • Department of Physics, Energy Studies and Nanotechnology School of Engineering and Energy, Murdoch University, Perth, Western Australia, Australia

  • Department of Agriculture and Food, State Government of Western Australia, Kensington, Western Australia, Australia

  • Department of Physics, Energy Studies and Nanotechnology School of Engineering and Energy, Murdoch University, Perth, Western Australia, Australia

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  • APA Style

    Gerrard Eddy Jai Poinern, Xuan Le, Mark O’Dea, Derek Fawcett. (2014). Can Anodic Aluminium Oxide Nanomembranes Treated with Nanometre Scale Hydroxyapatite be Used as a Cell Culture Substrate. International Journal of Materials Science and Applications, 3(6), 331-338. https://doi.org/10.11648/j.ijmsa.20140306.19

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    ACS Style

    Gerrard Eddy Jai Poinern; Xuan Le; Mark O’Dea; Derek Fawcett. Can Anodic Aluminium Oxide Nanomembranes Treated with Nanometre Scale Hydroxyapatite be Used as a Cell Culture Substrate. Int. J. Mater. Sci. Appl. 2014, 3(6), 331-338. doi: 10.11648/j.ijmsa.20140306.19

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    AMA Style

    Gerrard Eddy Jai Poinern, Xuan Le, Mark O’Dea, Derek Fawcett. Can Anodic Aluminium Oxide Nanomembranes Treated with Nanometre Scale Hydroxyapatite be Used as a Cell Culture Substrate. Int J Mater Sci Appl. 2014;3(6):331-338. doi: 10.11648/j.ijmsa.20140306.19

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  • @article{10.11648/j.ijmsa.20140306.19,
      author = {Gerrard Eddy Jai Poinern and Xuan Le and Mark O’Dea and Derek Fawcett},
      title = {Can Anodic Aluminium Oxide Nanomembranes Treated with Nanometre Scale Hydroxyapatite be Used as a Cell Culture Substrate},
      journal = {International Journal of Materials Science and Applications},
      volume = {3},
      number = {6},
      pages = {331-338},
      doi = {10.11648/j.ijmsa.20140306.19},
      url = {https://doi.org/10.11648/j.ijmsa.20140306.19},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.ijmsa.20140306.19},
      abstract = {In this study we investigate the biomedical potential of composite membranes composed of anodic aluminium oxide (AAO) and nanometre scale hydroxyapatite (HAP). The nano-porous AAO membranes were produced using a temperature controlled two-step anodization technique. The AAO/HAP composite membranes were formed using the solution template wetting technique. The Cercopithecus aethiops (African green monkey) Kidney (Vero) epithelial cell line was used to demonstrate the biocompatibility of the synthesised membranes and composites. Investigating cell adhesion, morphology and proliferation over a 72 h period assessed cellular interactions and responses of the cell line to the various membranes types.},
     year = {2014}
    }
    

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    AU  - Gerrard Eddy Jai Poinern
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    AB  - In this study we investigate the biomedical potential of composite membranes composed of anodic aluminium oxide (AAO) and nanometre scale hydroxyapatite (HAP). The nano-porous AAO membranes were produced using a temperature controlled two-step anodization technique. The AAO/HAP composite membranes were formed using the solution template wetting technique. The Cercopithecus aethiops (African green monkey) Kidney (Vero) epithelial cell line was used to demonstrate the biocompatibility of the synthesised membranes and composites. Investigating cell adhesion, morphology and proliferation over a 72 h period assessed cellular interactions and responses of the cell line to the various membranes types.
    VL  - 3
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