Evaluation of TSH, T4 and T3 in Human Serum: Standardization on Normal Individuals
American Journal of Modern Physics
Volume 2, Issue 4, July 2013, Pages: 202-207
Received: May 28, 2013;
Published: Jun. 20, 2013
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M. A. Samad, Department of Physics, Faculty of Science, University of Rajshahi, Rajshahi-6205, Bangladesh
M. M. Haque, Department of Physics, Faculty of Science, University of Rajshahi, Rajshahi-6205, Bangladesh
M. K. Shah, Center for Nuclear Medicine & Ultrasound, Rajshahi Medical College Hospital, Rajshahi, Bangladesh
M. R. Islam, Center for Nuclear Medicine & Ultrasound, Rajshahi Medical College Hospital, Rajshahi, Bangladesh
M. C. Mia, Department of Physics, Faculty of Science, University of Rajshahi, Rajshahi-6205, Bangladesh
A cross-sectional study of thyrotropin (TSH), thyroxine (T4) and triiodothyronine (T3) in human serum was performed aiming to define their normal values. In total, 201 assays were performed on 70 normal individuals, 26 men and 44 women, with ages ranging from 2 to 84 years. Serum TSH was measured with a highly sensitive immunoradiometric assay (IRMA) kit and T4 & T3 were determined with commercially available radioimmunoassay (RIA) kits. The normal values of TSH, T4 and T3 observed in the present study were 0.5 - 5.0 mIU/L, 55 - 169 nmol/L and 1.2 - 3.4 nmol/L, respectively, and their means (±SD) were 2.06±1.08 mIU/L, 113.21±27.62 nmol/L and 1.96±0.54 nmol/L, respectively. The results obtained were found to be in excellent agreement with the previously reported series. It is, therefore, expected that the present study will contribute some useful data in literature to set up normal values of serum TSH, T4 and T3.
M. A. Samad,
M. M. Haque,
M. K. Shah,
M. R. Islam,
M. C. Mia,
Evaluation of TSH, T4 and T3 in Human Serum: Standardization on Normal Individuals, American Journal of Modern Physics.
Vol. 2, No. 4,
2013, pp. 202-207.
S. Reza, Basic Human Anatomy and Physiology, Mediaplex publisher & Distributor: Dhaka, 2011, pp. 193.
W.F. Ganong, The Thyroid Gland in Review of Medical Phy sinology, The MeGraw Hill Companies: Newyork, 2011, pp. 307.
I.E. Griffin and S. R. Objeda, The Textbook of Endocrine Physiology, New York: Oxford University Press, 1988, pp.222 – 232.
T.E. Andreali, C.C. Bannet, F.C. Plum, Plum, Textbook of Medicine, 4th Edition, Philadelphia: Saunder & Co, 1997, p. 952–953.
R.K. Murry, P.K. Gerner, P.A. Mays, V.W. Harper's Rodwel, Textbook of Biochemistry, 25th Edition, Appleton & Lange: Stanford, 2000, pp. 8.
de Escobar G. Morreale. "The role of thyroid hormone in fetal neurodevelopment" J. Pediatr. Endocrinol Metab., 2001, vol. 14, No. (Suppl 6), pp. 1453-1462.
de Escobar G. Morreale, M. J. Obregon and del Rey F. Escobar. "Is neuropsychological development related to maternal hypothyroidism or to maternal hypothyroxinemia?" J. Clin. Endocrinol Metab., 2000, vol. 85, pp. 3975-3987.
G. Mirabella, D. Feig, E. Astzalos, K. Perlman and J.F. Rovet. "The effect of abnormal intrauterine thyroid hormone economies on infant cognitive abilities" J. Pediatr. Endocrinol Metab., 2000, vol. 13, pp. 191-194.
A.L. Dowling, E.A. Iannacone and R.T. Zoeller. Maternal hypothyroidism selectively affects the expression of neuroen-docrine-specific protein a messenger ribonucleic acid in the proliferative zone of the fetal rat brain cortex" Endocrinology, 2001, vol. 142, pp. 390-399.
P.A. Piosik, M. van Groenigen, J. van Doorn, F. Baas and J. J. de Vijlder. "Effects of maternal thyroid status on thyroid hormones and growth in congenitally hypothyroid goat fetuses during the second half of gestation endocrinology" 1997, vol. 138, pp. 5-11.
X. Y. Cao, X. M. Jiang, Z. H. Dou, M. A. Rakeman, M. L. Zhang, K. O’Donnell, T. Ma,K. Amette, N. DeLong and G. R. DeLong. "Timing of vulnerability of the brain to iodine deficiency in endemic cretinism" N. Engl. J. Med., 1994, vol. 331, pp. 1739-1744.
T. Vulsma, M. H. Gons and J. J. de Vijlder. "Maternal-fetal transfer of thyroxine in congenital hypothyroidism due to a total organification defect or thyroid agenesis" N. Engl. J. Med., 1989, vol. 321, pp. 13-16.
J. Bernal. "Action of thyroid hormone in brain" J. Endocrinol Invest., 2002. vol. 25, pp. 268-288.
C. C. Thompson and G. B. Potter. "Thyroid hormone action in neural development" Cereb. Cortex., 2000, vol. 10, pp. 939-945.
N. Koibuchi and W. W. Chin. "Thyroid hormone action and brain development trends" Endocrinol Metab, 2000, vol. 11, pp. 123-128.
P. C. Whybrow and M. Bauer, "Behavioral and psychiatric aspects of thyrotoxicosis," in The thyroid: fundamental and clinical text, L. E. Braverman and R. D. Utiger, Eds. Lippincott Williams and Wilkins: Phila-delphia, 2000), pp. 673.
S. C. Boyages, "The neuromuscular system and brain in thyrotoxicosis," in The thyroid: a fundamental and clinical text, L. E. Braverman, R. D. Utiger, Eds. Lippincott Williams and Wilkins, Philadelphia, 2000, pp. 631.
I. M. Baldini, A. Vita, M. C. Mauri, V. Amodei, M. Carrisi, S. Bravin and L. Cantalamessa. "Psychopathological and cognitive features in subclinical hypothyroidism" Prog. Neuropsy-chopharmacol Biol. Psychiatry., 1997. vol. 21, pp. 925-935.
D. Osterweil, K. Syndulko, S.N S. N. Cohen., P.D. P. D. Pettler-Jennings, J. M. Hershman, J. L. Cummings, W. W. Tourtellotte and D. H. Solomon. "Cognitive function in non-demented older adults with hypothyroidism" J. Am. Geriatr. Soc., 1992, vol. 40, pp. 325-335.
H. K. M. Yusuf, S. Quazi, M. R. Khan, M. Mahmuduzzaman, B. Nahar, M. M. Rahman, M. N. Islam, M. A. Khan, M. Shahidullah, I. Haque, M. Baquer and C. S. Panday. "Iodine deficiency disorders in Bangladesh" Indian J. Pediatr., 1996, vol. 63, pp. 105-110.
Q. Salamatullah, M. Rapid, H. K. M. Yusuf, N. Islam, M. N. Alam, M. A. Hasnat, M. Faruque et al. "Iodine disorder survey in Bangladesh" Dhaka University, 1999.
S. Glick. "Rosalyn sussman yalow (1921–2011)" Nature, 2011, vol. 474 (7353), pp. 580.
K. Y. Waite, C. F. Maberly and C. J. Eastman. "Immunoradiometric assay with use of magnetizable particles" Clin. Chem., 1986, vol. 32, No 10, pp. 1966-1968.
W. D. Odell, Z. Criffin and R. Zahradnik. "Two-monoclonal-antibody sandwish-type assay for thyrotropin with use of an avidin-biotin separation technique" Clin. Chem., 1986, vol. 32, No 10, pp. 1873-1878.
F. Thomas, Teddari and M. Edward Hoffmann. "Immunoradiometric assay for examination and quantitation of brucella abortus-specific antibodies reactive with the antigen(s) used in the indirect hemolysis test" J. Clin. Microbiol., 1981, vol.14, No 4, pp. 415-426.
A. V. Heal, "Radioassay," in Principles & practice of nuclear medicine, P. J. Early and D. B. Sodeel, Eds., Inc C.V. Mosby Company: St Louis, 1985, pp. 856.
Zahoor Ahmed, Mudassir Ahmad Khan, Amin ul Haq, Salma Attaullag and Zamil ur Rehman. "Effect of race, gender and age on thyroid and thyroid stimulating hormone levels in northwest frontier province, Pakistan" J Aub Med Coll abbottabad, 2009, vol. 21, No 9, pp. 21-24.
Pallavi Chaurasia, Bhautik Modi, Sarita Mangukiya, Pranay Jadav and Rita Shah. "Variation of thyroid hormones level among people of different age, gender and seasons, Piparia, Gujarat" National Journal of Medical Research, 2011, vol. 1, No 2, pp. 57-59.
I. N. Sills, M. N. B. Horlick and R. Rapaport. "Inappropriate suppression of thyrotropin during medical treatment of graves’ disease in childhood" J. Pediatr., 1992, vol. 163, pp. 262-263.
M. K. Shah, MPhil Thesis, University of Dhaka (Bangladesh), 2009.
Mary J. Shomon. "The TSH reference range wars: what’s "normal?" who is wrong, who is right, and what does it all mean for you and your health?" Sticking Out Our Necks, 2005, December 4, pp. 1-5.
A. Meller, T. J. Loy and A. J. Herle. "Diagnosis and management of solitary thyroid nodules" Br J Surg., 2002, vol. 78, pp. 90-93.
A. R. Spencer, B. M. Jaffe and J. C. Moron. "Hyperthyroidism and concurrent thyroid malignancies" Sur., 1990, vol. 106, pp. 6-10.
S. R. Joshi, "Laboratory evaluation of thyroid functions" JAPI, 2011, vol. 49, pp. 14-20.
M. C. Miah, MSc Thesis, University of Rajshahi (Bangladesh), 2012