Bacteriological Quality of Tilapia Fish from Treated Wastewater in Peri-Urban Areas, Morogoro, Tanzania
Agriculture, Forestry and Fisheries
Volume 5, Issue 5, October 2016, Pages: 202-206
Received: Aug. 16, 2016; Accepted: Sep. 5, 2016; Published: Oct. 6, 2016
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Authors
Ofred Jonas Mhongole, Department of Veterinary Medicine and Public Health, Sokoine University of Agriculture, Chuo Kikuu, Morogoro, Tanzania
Robinson Hammerthon Mdegela, Department of Veterinary Medicine and Public Health, Sokoine University of Agriculture, Chuo Kikuu, Morogoro, Tanzania
Lughano Jeromey Moses Kusiluka, Department of Veterinary Medicine and Public Health, Sokoine University of Agriculture, Chuo Kikuu, Morogoro, Tanzania; Office of the Vice Chancellor, Mzumbe University, Morogoro, Tanzania
Anders Dalsgaard, Department of Veterinary Disease Biology, Copenhagen University, Frederiksberg C, Denmark
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Abstract
The aim of this study was to assess faecal bacterial contamination in tilapia fish from wastewater treatment ponds at Mzumbe and in pristine water in Mindu dam. Tilapia fish (fish flesh and fish intestines) and water samples were analysed for Escherichia coli and total plate count. The concentration of E. coli in fish intestines ranged from about 1 - 3.5 log cfu/g and <1 log cfu/g in fish flesh. Total plate count ranged from 1 – 3 log cfu/g in fish flesh and 2 – 6 log cfu/g in fish intestines. The concentration of E. coli in pristine water in Mindu dam ranged from 0 to 15 cfu/mL. From the inlet to outlet of Mzumbe wastewater treatment pond, there was a significant reduction (p<0.05) of E. coli concentration along the gradient from 2.05 to 0.55 log cfu/mL, respectively. These findings demonstrated that tilapia fish from the two study sites were of good quality for human consumption based on microbial faecal contamination. On the other hand treated wastewater of the quality found in this study may be used for aquaculture. However, continuous monitoring for bacteriological quality and safety parameters including E. coli and total plate count is required.
Keywords
Escherichia coli, Fish Culture, Fish Flesh, Fish Intestines, Water
To cite this article
Ofred Jonas Mhongole, Robinson Hammerthon Mdegela, Lughano Jeromey Moses Kusiluka, Anders Dalsgaard, Bacteriological Quality of Tilapia Fish from Treated Wastewater in Peri-Urban Areas, Morogoro, Tanzania, Agriculture, Forestry and Fisheries. Vol. 5, No. 5, 2016, pp. 202-206. doi: 10.11648/j.aff.20160505.19
Copyright
Copyright © 2016 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
References
[1]
FAO 2005. Cultured Aquatic Species Information Programme Oreochromis niloticus. Text by Rakocy, J. E. In: FAO Fisheries and Aquaculture Department [online], Rome.
[2]
Kour, R. Bhatia, S. Sharma, K. K. 2014. Nile Tilapia (Oreochromis niloticus) as a successful biological invader in Jammu (J & K) and its impacts on native ecosystem, Int. J. Interdiscip. Multidiscip. Stud. 1: 1–5.
[3]
Bunting, S. W. 2004. Wastewater aquaculture: perpetuating vulnerability or opportunity to enhance poor livelihoods?, Aquat. Resour. Cult. Dev. 1:51–75.
[4]
Mizyed, N. R. 2012. Challenges to treated wastewater reuse in arid and semi-arid areas, Environ. Sci. Policy. 25: 186–195.
[5]
Sato, T. Qadir, M. Yamamoto, S. Endo, T. Zahoor, A. 2013. Global, Regional, and Country level need for data on wastewater generation, treatment, and use, Agric. Water Manag. 130: 1–13.
[6]
Mateo-Sagasta, J. Medlicott, K. Qadir, M. Raschid-Sally, L. Drechsel, P. Liebe, J. 2013. Proceedings of the UN-Water project on the Safe Use of Wastewater in Agriculture, In: R. Liebe, J., Ardakanian (Ed.), UN-Water Decad. Program. Capacit. Dev. (UNW-DPC). Proceedings Ser. No. 11., UNW-DPC, Bonn, Germany. pp. 1–79.
[7]
WHO. 2006. WHO Guidelines for the Safe Use of Wastewater, Excreta and Greywater: Volume III - Wastewater and Excreta Use in Aquaculture, Geneva, Swilzerland.
[8]
Valipour, M. 2013. Use of surface water supply index to assessing of water resources management in Colorado and Oregon, US, Adv. Agric. Sci. Eng. Res. 3: 631–640.
[9]
Valipour, M. 2012. Hydro-Module Determination for Vanaei Village in Eslam Abad Gharb, Iran, ARPN J. Agric. Biol. Sci. 7: 968–976.
[10]
Valipour, M. 2013. Evolution of irrigation-equipped areas as share of cultivated areas, Irrig. Drain. Syst. Eng. 02: 1–2.
[11]
Hossain, M.A. 2011. Fish as Source of n-3 Polyunsaturated Fatty Acids ( PUFAs ), Which One is Better-Farmed or Wild?, Adv. J. Food Sci. Technol. 3 (6): 455–466.
[12]
Buras, N. Duek, L. Niv, S. Hepher, B. Sandbank, E. 1987. Microbiological aspects of fish grown in treated wastewater, Water Res. 21: 1–10.
[13]
Budiati, T. Rusul, G. Wan-abdullah, W.N. Ahmad, R. Arip, Y. M. 2015. Microbiological Quality of Catfish (Clarias Gariepinus) and Tilapia (Tilapia Mossambica) Obtained from Wet Markets and Ponds in Malaysia, J Aquac Res Dev. 6: 1–5.
[14]
Ampofo, J. A. Clerk, G. C. 2010. Diversity of Bacteria Contaminants in Tissues of Fish Cultured in Organic Waste-Fertilized Ponds: Health Implications, Open Fish Sci. J. 3: 142–146.
[15]
Njoku, O. E. Agwa, O. K. Ibiene, A. A. 2015. An investigation of the microbiological and physicochemical profile of some fish pond water within the Niger Delta region of Nigeria, African J. Food Sci. 9: 155–162.
[16]
URT. 2007. The Environmental Management (Water Quality Standards) Regulations. The Environmental Management Act (CAP.191). United Republic of Tanzania.
[17]
Fox, B. Tamaru, C. Hollyer, J. Castro, L. 2012. A Preliminary Study of Microbial Water Quality Related to Food Safety in Recirculating Aquaponic Fish and Vegetable Production Systems, Food Saf. Technol. 51: 1–11.
[18]
ICMSF. 1986. Sampling for Microbiological Analysis: Principles and Specific Applications, In: Micro-Organisms Foods 2, Second ed, International Commission on Microbiological Specifications for Foods. Blackwell Scientific Publications. pp. 1–131.
[19]
Lan, N. T. P. Dalsgaard, A. Cam, P. D. Mara, D. 2007. Microbiological quality of fish grown in wastewater-fed and non-wastewater-fed fishponds in Hanoi, Vietnam: influence of hygiene practices in local retail markets, J. Water Health. 5: 209–218.
[20]
Silva, F. J. A. Mara, D. D. Pearson, H. W. Mota, S. E. 1997. Informal fish culture in the Maracanaú waste stabilisation ponds in Fortaleza, Brazil, Water Sci. Technol. 42: 393–398.
[21]
Dang, S. T. T. Dalsgaard, A. 2012. Escherichia coli Contamination of Fish Raised in Integrated Pig-Fish Aquaculture Systems in Vietnam, J. Food Prot. 75: 1317–1319.
[22]
Austin, B. 2006. The Bacterial Microflora of Fish, Revised, Sci. World J. 6: 931–945.
[23]
El-Shafai, S. A. Gijzen, H. J. Nasr, F. A. El-Gohary, F. A. 2004. Microbial quality of tilapia reared in fecal-contaminated ponds, Environ. Res. 95: 231–238.
[24]
Mkali, A. H. Ijumba, J. Njau, K.N. 2014. Effects of Wastewater Characteristics on Fish Quality from Integrated Wastewater Treatment System and Fish Farming in Urban Areas, Tanzania, Agric. For. Fish. 3: 292-298.
[25]
Ringø, E. Birkbeck, T. H. 1999. Intestinal microflora of fish larvae and fry, Aquac. Res. 30: 73–93.
[26]
Mandal, S. C. Hasan, M. Rahman, M.S. Manik, M.H. Mahmud, Z. H. Islam, M. D. S. 2009. Coliform bacteria in Nile tilapia, Oreochromis niloticus of shrimp-gher, pond and fish market, World J. Fish Mar. Sci. 1: 160–166.
[27]
Al-Harbi, A. H. 2003. Faecal coliforms in pond water, sediments and hybrid tilapia Oreochromis niloticus x Oreochromis aureus in Saudi Arabia, Aquac. Res. 34: 517–524.
[28]
Hussong, D. Damaré, J.M. Limpert, R. J. Sladen, W.J. Weiner, R. M. Colwell, R. R. 1979. Microbial impact of Canada geese (Branta canadensis) and whistling swans (Cygnus columbianus columbianus) on aquatic ecosystems., Appl. Environ. Microbiol. 37: 14–20.
[29]
Surendraj, A. Farvin, K. H. S. Yathavamoorthi, R. Thampuran, N. 2009. Enteric Bacteria Associated with Farmed Freshwater Fish and its Culture Environment in Kerala, India, Res. J. Microbiol. 4: 334–344.
[30]
Olugbojo, J. A. Ayoola, S. O. 2015. Comparative studies of bacterial load in fish species of commercial importance at the Aquaculture unit and lagoon front of the University of Lagos., Int. J. Fish. Aquac. 7: 37 – 43.
[31]
Al-Harbi, A. H. Uddin, N. 2005. Bacterial diversity of tilapia (Oreochromis niloticus) cultured in brackish water in Saudi Arabia, Aquaculture. 250 566–572.
[32]
Shinkafi, S. A. Ukwaja, V. C. 2010. Bacteria Associated with Fresh Tilapia Fish ( Oreochromis niloticus ) Sold at Sokoto Central Market in Sokoto, Nigeria., Niger. J. Basic Appl. Sci. 18: 217–221.
[33]
Turcios, A. E. Papenbrock, J. 2014. Sustainable treatment of aquaculture effluents-What can we learn from the past for the future?, Sustain. 6: 836–856.
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