Evaluation of Plant Growth Regulator, Immunity and DNA Fingerprinting of Biofield Energy Treated Mustard Seeds (Brassica juncea)
Agriculture, Forestry and Fisheries
Volume 4, Issue 6, December 2015, Pages: 269-274
Received: Oct. 11, 2015;
Accepted: Oct. 19, 2015;
Published: Nov. 16, 2015
Views 5807 Downloads 94
Mahendra Kumar Trivedi, Trivedi Global Inc., Henderson, USA
Alice Branton, Trivedi Global Inc., Henderson, USA
Dahryn Trivedi, Trivedi Global Inc., Henderson, USA
Gopal Nayak, Trivedi Global Inc., Henderson, USA
Sambhu Charan Mondal, Trivedi Science Research Laboratory Pvt. Ltd., Bhopal, Madhya Pradesh, India
Snehasis Jana, Trivedi Science Research Laboratory Pvt. Ltd., Bhopal, Madhya Pradesh, India
Among the oilseeds grown around the world, mustard is one of the important crop worldwide due to its wide adaptability and high yielding capacity. Owing to the importance of its utilities as condiment, cooking oil and some medical aids, the demand for its seed production is too high. The present study was carried out to evaluate the impact of Mr. Trivedi’s biofield energy treatment on mustard (Brassica juncea) for its growth-germination of seedling, glutathione (GSH) content in leaves, indole acetic acid (IAA) content in shoots and roots and DNA polymorphism by random amplified polymorphic-DNA (RAPD). The sample of B. juncea was divided into two groups. One group was remained as untreated and coded as control, while the other group was subjected to Mr. Trivedi’s biofield energy treatment and referred as the treated sample. The growth-germination of B. juncea seedling data exhibited that the biofield treated seeds were germinated faster on day 5 as compared to the control (on day between 7-10). The shoot and root length of seedling were slightly increased in the treated seeds of 10 days old with respect to untreated seedling. Moreover, the major plant antioxidant i.e. GSH content in mustard leaves was significantly increased by 206.72% (p<0.001) as compared to the untreated sample. Additionally, the plant growth regulatory constituent i.e. IAA level in root and shoot was increased by 15.81% and 12.99%, respectively with respect to the control. Besides, the DNA fingerprinting data using RAPD revealed that the treated sample showed an average 26% of DNA polymorphism as compared to the control. The overall results envisaged that the biofield energy treatment on mustard seeds showed a significant improvement in germination, growth of roots and shoots, GSH and IAA content in the treated sample. In conclusion, the biofield energy treatment of mustard seeds could be used as an alternative way to increase the production of mustard.
Mahendra Kumar Trivedi,
Sambhu Charan Mondal,
Evaluation of Plant Growth Regulator, Immunity and DNA Fingerprinting of Biofield Energy Treated Mustard Seeds (Brassica juncea), Agriculture, Forestry and Fisheries.
Vol. 4, No. 6,
2015, pp. 269-274.
Dubie J, Stancik A, Morra M, Nindo C (2013) Antioxidant extraction from mustard (Brassica juncea) seed meal using high-intensity ultrasound. J Food Sci 78: E542-E548.
Tsuruo I, Yoshida M, Hata T (1967) Studies on the myrosinase in mustard seed part I. the chromatographic behaviors of the myrosinase and some of its characteristics. Agr Biol Chem 31: 18-26.
Bones AM, Rossiter JT (1996) The myrosinase-glucosinolate system, its organization and biochemistry. Physiol Plant 97: 194-208.
Bassan P, Sharma S, Arora S, Vig AP (2013) Antioxidant and in vitro anti-cancer activities of Brassica juncea (L.) Czern. seeds and sprouts Int J Pharma Sci 3: 343-349.
Amarowicz R, Wanasundara UN, Karamac M, Shahidi F (1996) Antioxidant activity of ethanolic extract of mustard seed. Nahrung 40: 261-263.
Rance F, Dutau G, Abbal M (2000) Mustard allergy in children. Allergy 55: 496-500.
Morisset M, Moneret-Vautrin DA, Maadi F, Fremont S, Guenard L, et al. (2003) Prospective study of mustard allergy: first study with double-blind placebo-controlled food challenge trials (24 cases). Allergy 58: 295-299.
Parti RS, Deep V, Gupta SK (2003) Effect of salinity on lipid components of mustard seeds (Brassica juncea L.) Plant Food Hum Nutr 58: 1-10.
Munns R, Tester M (2008) Mechanisms of salinity tolerance. Ann Rev Plant Biol 59: 651-681.
Dionisio-Sese ML, Tobita S (1998) Antioxidant responses of rice seedlings to salinity stress. Plant Sci 135: 1-9.
Mittler R, Vanderauwera S, Gollery M, Van Breusegem F (2004) Reactive oxygen gene network of plants. Trends Plant Sci 9: 490-498.
Asada K (2006) Production and scavenging of reactive oxygen species in chloroplasts and their functions. Plant Physiol 141: 391-396.
Noctor G, Foyer CH (1998) Ascorbate and glutathione: Keeping active oxygen under control. Annu Rev Plant Physiol Plant Mol Biol 49: 249-729.
Khan MA, Rabbani MA, Munir M, Ajmal SK, Malik MA (2008) Assessment of genetic variation within Indian mustard (Brassica juncea) germplasm using random amplified polymorphic DNA markers. J Integr Plant Biol 50: 385-392.
Barnes PM, Powell-Griner E, McFann K, Nahin RL (2004) Complementary and alternative medicine use among adults: United States, 2002. Adv Data 343: 1-19.
Shinde V, Sances F, Patil S, Spence A (2012) Impact of biofield treatment on growth and yield of lettuce and tomato. Aust J Basic Appl Sci 6: 100-105.
Sances F, Flora E, Patil S, Spence A, Shinde V (2013) Impact of biofield treatment on ginseng and organic blueberry yield. Agrivita J Agric Sci 35.
Lenssen AW (2013) Biofield and fungicide seed treatment influences on soybean productivity, seed quality and weed community. Agricultural Journal 8: 138-143.
Nayak G, Altekar N (2015) Effect of biofield treatment on plant growth and adaptation. J Environ Health Sci 1: 1-9.
Trivedi MK, Tallapragada RM, Branton A, Trivedi D, Nayak G, et al. (2015) Characterization of physical, spectral and thermal properties of biofield treated 1,2,4-Triazole. J Mol Pharm Org Process Res 3: 128.
Moron MS, Depierre JW, Mannervik B (1979) Levels of glutathione, glutathione reductase and glutathione S-transferase activities in rat lung and liver. Biochim Biophys Acta 582: 67-78.
Tang YW, Bonner J (1947) The enzymatic inactivation of indoleacetic acid. I. Some charasteristics of the enzyme contained in pea seedlings. Arch Biochem 13: 11-25.
Green MR, Sambrook J (2012) Molecular cloning: A laboratory manual. (3rdedn), Cold Spring Harbor, N.Y. Cold Spring Harbor Laboratory Press.
Borges A, Rosa MS, Recchia GH, QueirozSilva JRD, Bressan EDA, et al. (2009) CTAB methods for DNA extraction of sweet potato for microsatellite analysis. Sci Agric (Piracicaba Braz) 66: 529-534.
Welsh JW, McClelland M (1990) Fingerprinting genomes using PCR with arbitrary primers. Nucleic Acids Res 18: 7213-7218.
Turk MA, Tawaha AM (2002) Inhibitory effects of aqueous extracts of barley on germination and growth of lentil. Pak J Agron 1: 28-30.
Sharma P, Sardana V, Banga SS (2013) Salt tolerance of Indian mustard (Brassica juncea) at germination and early seedling growth. Environ Exp Biol 11: 39-46.
Rausch T, Wachter A (2005) Sulfur metabolism: A versatile platform for launching defense operations. Trend Plant Sci 10: 503-509.
Gagandeep, Dhiman M, Mendiz E, Rao AR, Kale RK (2005) Chemopreventive effects of mustard (Brassica compestris) on chemically induced tumorigenesis in murine forestomach and uterine cervix. Hum Exp Toxicol 24: 303-312.
Zhu YL, Pilon-Smits EAH, Jouanin L, Terry N (1999) Overexpression of glutathione synthetase in Indian mustard enhances cadmium accumulation and tolerance. Plant Physiol 119: 73-80.
Black H (1995) Absorbing possibilities: Phytoremediation. Environ Health Perspect 103: 1106-1108.
Yamada T (1993) The role of auxin in plant-disease development. Annu Rev Phytopathol 31: 253-273.
Morris RO (1986) Genes specifying auxin and cytokinin biosynthesis in phytopathogens. Annu Rev Plant Physiol 37: 509-538.
Chen Q, Qi WB, Reiter RJ, Wei W, Wang BM (2009) Exogenously applied melatonin stimulates root growth and raises endogenous indoleacetic acid in roots of etiolated seedlings of Brassica juncea. J Plant Physiol 166: 324-328.
Kimura M (1983) The neutral theory of molecular evolution. Cambridge Univ. Press, Cambridge.
Bretting PK, Widrlechner MP (1995) Genetic markers and plant genetic resource management. John Wiley & Son Inc. Canada.
Williams JG, Kubelik AR, Livak KJ, Rafalski JA, Tingey SV (1990) DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res 18: 6531-6535.