Histo-Cytological Study of the Graft union of the Chestnut (Castanea Sativa Mill)/Oak (Quercus Vulcanica Boiss)
Agriculture, Forestry and Fisheries
Volume 2, Issue 2, April 2013, Pages: 110-115
Received: Apr. 8, 2013; Published: May 20, 2013
Views 3298      Downloads 247
Selime ADA, Adnan Menderes University, Faculty of Agriculture, Department of Horticulture, 09100 Aydın, Turkey
Engin ERTAN, Adnan Menderes University, Faculty of Agriculture, Department of Horticulture, 09100 Aydın, Turkey
Article Tools
Follow on us
Graft compatibility is a complex mechanism with a wide range of different physiological, biochemical, and anatomical interactions. In the present paper, features of the graft union on the combination of the European chestnut (Castanea sativa Mill.) on an oak (Quercus vulcanica Boiss.) rootstock based on whip grafting was investigated. The trial was conducted in the greenhouse at the Faculty of Agriculture, Adnan Menderes University, in western Turkey during 2006-7. The grafts were made with scion from chestnut genotype N-3-4 on an oak rootstock. Cross and longitudinal sections of the graft union for examination were taken 30, 60, 90, 120, 150 and 210 days after grafting, and fixed in 70% ethanol. Microscopic observation of the anatomical and histo-cytological structure of the graft union area revealed that new cambium, xylem, and phloem tissues were formed in the samples 120 days after grafting. The results showed that, graft compatibility exists between chestnut and oaks. Callus formation and differentiation of vascular tissues started 150 days after grafting.
Chestnut, Oak, Anatomy, Histology, Graft Union
To cite this article
Selime ADA, Engin ERTAN, Histo-Cytological Study of the Graft union of the Chestnut (Castanea Sativa Mill)/Oak (Quercus Vulcanica Boiss), Agriculture, Forestry and Fisheries. Vol. 2, No. 2, 2013, pp. 110-115. doi: 10.11648/j.aff.20130202.18
Barnett, J. R. and I. Weatherhead, 1988. Graft formation in Sitka spruce: a scanning electron microscopy study. Annals of Botany. 61, 581-587.
Cohen, R., Burger, Y., Koern, A., Edelstein, M., 2007. Introducing grafted cucurbitsto modern agriculture; The Israeli experience. Plant Disease. 91, 916–923.
Craddock, J. H. and G. Bassi, 1999. Effect of clonally propagated interspecific hybrid chestnut rootstock on short-term graft compatibility with four cultivars of Italian "Marrone". Acta Horticulturae (ISHS), 494: 451-458.
Errea, P., A. Felipe and M. Herrero, 1994b. Graft establishment between compatible and incompatible Prunus spp. Journal of Expermantal Bottany. 45, 393-401.
Errea, P., D. Treutter and W. Feucht, 1994a. Characterization of flavanol-type polyphenols in apricot cultivar and rootstock. Advences in Horticulturel Science. 3, 165-169.
Errea, P., L. Garay and J. A. Marin, 2001. Early detection of graft incompatibility in apricot (Prunus armeniaca) using in vitro techniques. Physiol. Plant. 112, 135-141.
Ertan, E., G. Seferoğlu, G. G. Dalkılıç, F. E. Tekintaş, S. Seferoğlu, F. Babaeren, M. Önal and Z. Dalkılıç, "Selection of Chestnuts (Castanea sativa Mill.) Grown in Nazilli District, Turkey", Turkish Journal of Agriculture and Forestry, 31 (2), 115-123 (2007).
Feutch, W., 1988. Graft incompatibility of tree crops: An overview of the present scientific status. Acta Horticulturae 227: 33-41.
Goldsbrough, G., 1990. A beginner’s guide to chestnut growing. Hilton press, Auckland, New Zealand, 40pp.
Hartmann, H. T., D. E. Kester and F. T. Davies, 1990. Plant Propagation. Principles and Practices, 5th edn. Prentice-Hall, 647 pp.
Heitz, R. and C. Jacquiot, 1972. Etude anatomıque de la greffe d’un chataıgnıer sur chene. Annals of Forest Science, 29 (3), 391- 395.
Huang, H., J. D. Norton, G.E. Boyhan, and B.R. Abrahams, 1994. Graft compatibility among chestnut (Castanea) species. Journal of the American Society for Horticultural Science 119 (6): 1127–1132.
Jefree, C. E. and M. M. Yeoman, 1983. Development of intercellular connections between opposing cells in a graft union. New Phytologist. 93, 491-509.
McCully, M.E. 1983. Structural aspects of graft development. p.71-88 In: R. Moore (Editor). Vegetative Compatibility Responses in Plants. Baylor Univ. Press, Waco, TX.
Moore, R. and D. B. Walker, 1981: Studies of vegetative compatibility-incompatibility in higher plants. I. A Structural study of a compatible autograft in Sedum telephoides (Crassulaceae). American Journal of Botany, 68, (6): 820-830.
Mosse, B., 1962. Graft incompatibility in fruit trees. Technical Communication of the Commonwealth Bureau of Horticultural Plant Crops, 28: 1-36.
Oraguzie, N. C., D. L. McNeil and M. B. Thomas, 1998. Examination of graft failure in New Zealand chestnut (Castanea spp) selections. Scientia Horticulturae, 76: 89-103.
Özçağıran, R. 1974. Physiological Relationships on Rootstock and Scion on Fruit Trees. Ege University Faculty of Agriculture, No: 243, İzmir. (In Turkish)
Park, K.S. 1968. Studies on the juvenile tissue grafting of some special use- trees III. On the modified nurse seed grafting of some crop tree species (chestnut, ginkgo and oak). Res. Rpt. Inst. For. Gen. Korea 6:89-104.
Pina, A. and P. Errea, 2005. A review of new advances in mechanism of graft compatibility-incompatibility. Scientia Horticulturae, 106: 1-11.
Rutter, P. A., G. Miller and J. A. Payne, 1990. Chestnuts (Castanea). Acta Horticulturae. 20: 27-33.
Santamour, F.S. 1988. Graft incompatibility related to cambial peroxidaz isozymes in Chinese chestnut. Journal of Enviromental Horticulture, 6 (2), p. 33–39.
Seferoglu, G. and E. Ertan, 2003. Callus and graft union formation in Turkish chestnut selections. Bulletin of Pure Applied Science, 22B (2): 95-104.
Serdar, Ü. and A. Soylu, 2004. Investigation of anatomical structure of graft union for T and inverted T buddings and whip grafting in chestnut. Acta Horticulturae (ISHS), 693: 165-170
Serdar, Ü., B. Köse and F. Yilmaz, 2005. The structure of Graft Unions in European Chestnut Using Different Grafting Methods. Hortscience, 40 (5): 1474-1477.
Ufuk, S. and A. Soylu, 1999. Researches on stock-scion compatibility between some important chestnut cultivars and hybrid rootstocks. Acta Horticulturae, 494: 223-229.
Science Publishing Group
1 Rockefeller Plaza,
10th and 11th Floors,
New York, NY 10020
Tel: (001)347-983-5186