American Journal of Life Sciences

| Peer-Reviewed |

Ex Situ Biodegradation of Crude Oil Using Bacterial Isolates From Palm Oil Mill Effluent

Received: 18 May 2016    Accepted: 27 May 2016    Published: 14 June 2016
Views:       Downloads:

Share This Article

Abstract

Bioremediation of crude oil is quite unique, complex and relatively a slow process. They study area for this research work was a cottage industry located in Orumba North local government area of Anambra state, Nigeria. Evaluating the degradation potential of bacterial isolates from palm oil mill effluent on crude oil was conducted using standard methods, which includes screening, inoculums development and degradation studies to assess the most promising hydrocarbon degraders. Pooled samples collected from different points on the palm oil mill effluent disposal channels were inoculated mineral salt medium containing crude oil to assess degradation abilities of different bacterial species. Data indicated that these bacterial isolates were capable of thriving in the mineral salt medium between the pH range of 6.0-7.0 and bacterial proliferation were quantified by assessing the total viable count which ranged between 2 x 105-9 x 105 cfu/ml based on individual abilities to utilize crude oil as a sole carbon source. Cultural, morphological and biochemical characterizations were conducted on these isolates and Bergey’s manual of determinative bacteriology was used to suggest possible isolates, while molecular identification was done Centre for Agriculture and biosciences International (CABI), UK using partial 16S rDNA sequencing analysis and FASTA algorithm with prokaryote database from EBI. Escherichia fergosonii, Klebsiella variicola and Microccocus luteus were identified. Gas chromatographic analysis was applied to quantify the extent of degradation of crude oil by the isolates. All the isolated bacterial species displayed varying degree of crude hydrocarbon biodegradation.

DOI 10.11648/j.ajls.20160403.11
Published in American Journal of Life Sciences (Volume 4, Issue 3, June 2016)
Page(s) 71-75
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Palm Oil Mill Effluent, Crude Oil, Biodegradation, Bacteria, Ex Situ

References
[1] Kvenvolden K. A. Cooper C. K.. Natural seepage of crude oil into the marine environment”, Geo- marine letters. Vol. 23, 2003, pp. 140-146.
[2] Tanaka, O. H., Venkatswaran, K., Komukai, S., Toki, H., Iwabuchi, T. And Miyachi, S. Ecodynamics of oil degrading bacterial and significance of marine mixed population in the degradation of petroleum compounds. International oil spill conference. 1993, pp. 780-785.
[3] Yoshida, M. H. Michel, L. A. Sazanov, S. Yoshikawa, J. Barber, J. K. Lanyi, S. P. Balashov, V. A. Shuvalov, A. G. Yakovlev, T. A. Shkuropatova, L. G. Vasiheva, A. Y. Shkuropatov, P. Gast, S. D. Dunn, P. A. Delrizzo, Y. Bi, K. S. Wood, D. J. Cipriano, P. Turina, A. Biochimica et Biophsica Acta (BBA)-Bioenergetics, 2006, Vol. 1757, 151-155.
[4] Odu, C. I. I. Microbiology of soil contaminated with petroleum hydrocarbons. The extent of contamination of some soils and microbial properties after contamination. Journal of Institute of Petroleum. 1972, vol 58, pp. 202-208.
[5] Jameel, A. T, Olanrewwaju, A. A. Aerobic biodegradation of oil and grease in palm oil mill effluent using consortium of microorganisms. In: M. D. Z, Alam, A. T, Jameel and A, Amid (eds). Current research and development in biotechnology engineering at International Islamic University Malaysia (IIUM) Vol. III. IIUM Press, Kula Lumpur. 2011, pp. 43-51. ISBN 978967418444
[6] Nwankwegu, A. S., Onwosi, C. O., Orji, M. U., Anaukwu, C. G., Okafor, U. C., Azi, F., Marins, P. E. Reclamation of DPK hydrocarbon polluted agricultural soil using a selected bulking agent. Journal of Environmental Management. 2016, Vol. 172, pp. 136-142.
[7] Obayori, O. S., Ilori, M. O., O. S., Ilori., Adebusoye, M. O., Amund, O. O. and Oyetibe. Microbial population changes in tropical agricultural soil experimentally contaminated with crude oil petroleum. African Journal of Biotechnology. 2008, pp. 4512-4520.
[8] Okpokwasili, G. C and Okori, B. B. Biodeterioration potentials of Microorganisms isolated from car engine lubricating oil. Tribology International. 1988, Vol. 21, pp. 215-220.
[9] Obuekwe, C. O and Al-zarban. Bioremediation of crude oil polluted in Kuwaiti desert: The role of adherent microorganisms, Environmental International. 2011, Vol. 24, pp. 832-834.
[10] Bartha, R and Atlas, R. M. The Microbiology of Aquatic oil spills. Advance Applied Microbiology Vol. 22, pp. 225-226.
[11] Calomiris, J. T., Austin, B, Walker, J. D. and Colwell, R. R. Enrichment for Estuarine. Petroleum Degrading Bacterial using liquid and solid media. Journal of Applied Bacteriology. 1976. Vol. 41, pp. 135-144.
[12] Pacwa-Plociniczak, M., Plaza, G. A., Pitrowska-seget, Z. M. Monitoring the changes in a bacterial community in petroleum-polluted soil bioaugmented with hydrocarbon-degrading strains. Applied Soil Ecology. 2016, Vol. 105, pp. 76-85.
[13] Stordal, I. F., Olsen, A. J., Jenssen, B. M., Netzer, R., Hansen, B. H., Altin, D., Braskstad, O. G. Concentrations of viable oil-degrading microorganisms are increased in feces from Calanus finmarchicus feeding in petroleum oil dispersions. Marine pollution Bulletin. 2015, Vol. 98, pp. 69-77.
[14] Ntougias, S., Melidis, P., Navrozidou, E., Tzegkas, F. Diversity and efficiency of anthracene-degrading bacteria isolated from a denitrifying activated sludge system treating municipal wastewater. International Biodeterioration & Biodegradation. 2015, Vol. 97, pp. 151-158.
[15] Kurachi, K., Hosokawa, R., Takahashi, M., Okuyama, H. The potential of glycerol in freezing preservation of turbine oil-degrading bacterial consortium and the ability of the revised consortium to degrade petroleum wastes. International Biodeterioration & Biodegradation. 2014, Vol. 88, pp. 77-82.
Author Information
  • Department of Applied Microbiology and Brewing, Nnamdi Azikiwe University, Awka, Anambra State, Nigeria

  • Department of Applied Microbiology and Brewing, Nnamdi Azikiwe University, Awka, Anambra State, Nigeria

  • Department of Applied Microbiology and Brewing, Nnamdi Azikiwe University, Awka, Anambra State, Nigeria

  • Department of Applied Microbiology and Brewing, Nnamdi Azikiwe University, Awka, Anambra State, Nigeria

  • Department of Applied Microbiology and Brewing, Nnamdi Azikiwe University, Awka, Anambra State, Nigeria

Cite This Article
  • APA Style

    Amechi Sampson Nwankwegu, Emmanuel Michael Ikpe, Edna Ifeoma Chukwura, Richard Chidiebere Irondi, Emmanuel Ifeanyi Obika. (2016). Ex Situ Biodegradation of Crude Oil Using Bacterial Isolates From Palm Oil Mill Effluent. American Journal of Life Sciences, 4(3), 71-75. https://doi.org/10.11648/j.ajls.20160403.11

    Copy | Download

    ACS Style

    Amechi Sampson Nwankwegu; Emmanuel Michael Ikpe; Edna Ifeoma Chukwura; Richard Chidiebere Irondi; Emmanuel Ifeanyi Obika. Ex Situ Biodegradation of Crude Oil Using Bacterial Isolates From Palm Oil Mill Effluent. Am. J. Life Sci. 2016, 4(3), 71-75. doi: 10.11648/j.ajls.20160403.11

    Copy | Download

    AMA Style

    Amechi Sampson Nwankwegu, Emmanuel Michael Ikpe, Edna Ifeoma Chukwura, Richard Chidiebere Irondi, Emmanuel Ifeanyi Obika. Ex Situ Biodegradation of Crude Oil Using Bacterial Isolates From Palm Oil Mill Effluent. Am J Life Sci. 2016;4(3):71-75. doi: 10.11648/j.ajls.20160403.11

    Copy | Download

  • @article{10.11648/j.ajls.20160403.11,
      author = {Amechi Sampson Nwankwegu and Emmanuel Michael Ikpe and Edna Ifeoma Chukwura and Richard Chidiebere Irondi and Emmanuel Ifeanyi Obika},
      title = {Ex Situ Biodegradation of Crude Oil Using Bacterial Isolates From Palm Oil Mill Effluent},
      journal = {American Journal of Life Sciences},
      volume = {4},
      number = {3},
      pages = {71-75},
      doi = {10.11648/j.ajls.20160403.11},
      url = {https://doi.org/10.11648/j.ajls.20160403.11},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.ajls.20160403.11},
      abstract = {Bioremediation of crude oil is quite unique, complex and relatively a slow process. They study area for this research work was a cottage industry located in Orumba North local government area of Anambra state, Nigeria. Evaluating the degradation potential of bacterial isolates from palm oil mill effluent on crude oil was conducted using standard methods, which includes screening, inoculums development and degradation studies to assess the most promising hydrocarbon degraders. Pooled samples collected from different points on the palm oil mill effluent disposal channels were inoculated mineral salt medium containing crude oil to assess degradation abilities of different bacterial species. Data indicated that these bacterial isolates were capable of thriving in the mineral salt medium between the pH range of 6.0-7.0 and bacterial proliferation were quantified by assessing the total viable count which ranged between 2 x 105-9 x 105 cfu/ml based on individual abilities to utilize crude oil as a sole carbon source. Cultural, morphological and biochemical characterizations were conducted on these isolates and Bergey’s manual of determinative bacteriology was used to suggest possible isolates, while molecular identification was done Centre for Agriculture and biosciences International (CABI), UK using partial 16S rDNA sequencing analysis and FASTA algorithm with prokaryote database from EBI. Escherichia fergosonii, Klebsiella variicola and Microccocus luteus were identified. Gas chromatographic analysis was applied to quantify the extent of degradation of crude oil by the isolates. All the isolated bacterial species displayed varying degree of crude hydrocarbon biodegradation.},
     year = {2016}
    }
    

    Copy | Download

  • TY  - JOUR
    T1  - Ex Situ Biodegradation of Crude Oil Using Bacterial Isolates From Palm Oil Mill Effluent
    AU  - Amechi Sampson Nwankwegu
    AU  - Emmanuel Michael Ikpe
    AU  - Edna Ifeoma Chukwura
    AU  - Richard Chidiebere Irondi
    AU  - Emmanuel Ifeanyi Obika
    Y1  - 2016/06/14
    PY  - 2016
    N1  - https://doi.org/10.11648/j.ajls.20160403.11
    DO  - 10.11648/j.ajls.20160403.11
    T2  - American Journal of Life Sciences
    JF  - American Journal of Life Sciences
    JO  - American Journal of Life Sciences
    SP  - 71
    EP  - 75
    PB  - Science Publishing Group
    SN  - 2328-5737
    UR  - https://doi.org/10.11648/j.ajls.20160403.11
    AB  - Bioremediation of crude oil is quite unique, complex and relatively a slow process. They study area for this research work was a cottage industry located in Orumba North local government area of Anambra state, Nigeria. Evaluating the degradation potential of bacterial isolates from palm oil mill effluent on crude oil was conducted using standard methods, which includes screening, inoculums development and degradation studies to assess the most promising hydrocarbon degraders. Pooled samples collected from different points on the palm oil mill effluent disposal channels were inoculated mineral salt medium containing crude oil to assess degradation abilities of different bacterial species. Data indicated that these bacterial isolates were capable of thriving in the mineral salt medium between the pH range of 6.0-7.0 and bacterial proliferation were quantified by assessing the total viable count which ranged between 2 x 105-9 x 105 cfu/ml based on individual abilities to utilize crude oil as a sole carbon source. Cultural, morphological and biochemical characterizations were conducted on these isolates and Bergey’s manual of determinative bacteriology was used to suggest possible isolates, while molecular identification was done Centre for Agriculture and biosciences International (CABI), UK using partial 16S rDNA sequencing analysis and FASTA algorithm with prokaryote database from EBI. Escherichia fergosonii, Klebsiella variicola and Microccocus luteus were identified. Gas chromatographic analysis was applied to quantify the extent of degradation of crude oil by the isolates. All the isolated bacterial species displayed varying degree of crude hydrocarbon biodegradation.
    VL  - 4
    IS  - 3
    ER  - 

    Copy | Download

  • Sections