Variations in Biochemical Attributes of Cassia tora L. and C. auriculata L. under Temperature Stress
American Journal of Life Sciences
Volume 2, Issue 6-1, November 2014, Pages: 16-21
Received: Aug. 16, 2014;
Accepted: Sep. 3, 2014;
Published: Sep. 17, 2014
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Geetika Pant, Department of Biotechnology, Indian Academy Degree College, Centre for Research and Post Graduate Studies, Bangalore, INDIA
Sibi G., Department of Biotechnology, Indian Academy Degree College, Centre for Research and Post Graduate Studies, Bangalore, INDIA
Sangeetha Annie George, Department of Zoology, Indian Academy Degree College, Centre for Research and Post Graduate Studies, Bangalore, INDIA
Shubha Bhadran, Department of Genetics, Indian Academy Degree College, Centre for Research and Post Graduate Studies, Bangalore, INDIA
Ugam Chauhan, Department of Biotechnology, A. P. S. University, Madhya Pradesh, INDIA
Plants continuously struggle for survival under various environmental abiotic stress conditions, specifically high temperature. Fourteen-day-old seedlings of Cassia tora and Cassia auriculata were subjected to differential temperature stress treatments at 30ºC, 37ºC, 42°C and 44°C for 16 h each. Various biochemical parameters viz reducing sugars, total protein, chlorophyll content and antioxidant enzyme system were assessed and found to be increased under high temperature stress. The amount of reducing sugars, total protein and chlorophyll were remarkably increased in both the Cassia species at 42ºC. The POX activity was more profound in C. tora (0.41 U/mg) than C. auriculata (0.24 U/mg) at 42°C. However, the activity of Catalase in both the species recorded a similar effect with a maximum value of 0.39 and 0.43 U/mg in C. tora and C. auriculata respectively. Similarly, SOD percentage inhibition activity increased significantly at 42°C for C. tora and C. auriculata showing a noticeable trend of inhibition of 85.23% and 86.89% respectively. Thus it can be concluded that various osmolytes and an efficient antioxidative system play a key role in generating tolerance against temperature stress and maintaining homeostasis to withstand the maximum range for survival at 42ºC in Cassia seedlings.
Sangeetha Annie George,
Variations in Biochemical Attributes of Cassia tora L. and C. auriculata L. under Temperature Stress, American Journal of Life Sciences. Special Issue: Recent Developments in Health Care through Plants and Microbes.
Vol. 2, No. 6-1,
2014, pp. 16-21.
W. Wang, B. Vinocur, A. Altman, “Plant responses to drought, salinity and extreme temperatures towards genetic engineering for stress tolerance,” Planta, vol. 218, 1-14, 2007.
M.M. Chaves, J.P. Maroco, J.S. Pereira, “Understanding plant responses to drought from genes to the whole plant,” Funct. Plant Biol., vol. 30, 239-264, 2003.
S. Kotak, E. Vierling, H. Baumlein, P. Von Koskull-Doring, “A novel transcriptional cascade regulating expression of heat stress proteins during seed development of Arabidopsis,” Plant Cell, vol.19, 182-195, 2007a.
S. Kotak, J. Larkindale, U. Lee, P. Von Koskull-Doring, E. Vierling, K.D. Scharf, “Complexity of the heat stress response in plants,” Curr. Opin. Plant Biol, vol. 10, 310-316, 2007b.
W. Wang, B. Vinocur, O. Shoseyov, A. Altman, “Role of plant heat shock proteins and molecular chaperons in the abiotic stress response,” Trends in plant Science. vol. 9, 244-252, 2004.
K.V. Sumesh , P. Sharma-Natu, M.C. Ghildiyal, “Starch synthase activity and heat shock protein in relation to thermal tolerance of developing wheat grains,” Biol. Planta. vol.52, 749-753, 2008.
M.G Santos, R.V. Ribeiro, E.C. Machado, C. Pimentel, “Photosynthetic parameters and leaf water potential of five common bean genotypes under mild water deficit,” Biol. Planta., vol. 53, 2009, pp. 229-236, 2009.
B. Grigorova, I. Vaseva, K. Demrievska, U. Feller, “Combined drought and heat stress in wheat: changes in some heat shock proteins” Biol. Planta., vol. 55, 105-111, 2011.
C.D. Giaveno, J. Ferrero, “Introduction of tropical maize genotypes to increase silage production in the central area of Santa Fe, Argentina,” Crop Breed Appl. Biotech., vol. 3, 203-208, 2008.
J.H. Zhang, W.D. Huang, Y.P. Liu, Q.H. Pan, “Effect of temperature acclimation pretreatment on the ultra structure of mesophyll cells in young grape plants (Vitis vinifera) under cross temperature stresses,” J. Integ. Plant. Bio., vol. 47, 959-970, 2005.
J.L. Simoes-Araujo, N.G. Rumjanek, M. Margis-Pinheiro, “Small heat shock proteins genes are differentially expressed in distinct varieties of common bean,” Braz. J. Plant Physiol., vol. 15, 33-41, 2003.
P. Morales, J. Pace, J. Platt, T. Phillips, K. Morgan, A. Fazleabas, J. Hunt, “Placental cell expression of HLA-G2 isoforms is limited to the invasive trophoblast phenotype,” J Immunol , vol. 171, 6215-6224, 2003.
A.I. Cabanero, Y. Madrid, C. Camara, “Selenium and mercury bioaccessibility in fish samples: an in vitro digestion method,” Analytica Chimica Acta, vol. 526, 51-61, 2004.
P.D. Hare, W. Cresss, “A metabolic implication of stress-induced proline accumulation in plants,” Plant growth regulation., vol. 21, 79-102, 1998.
A. Sakamoto, N. Murata, “The role of glycine betaine in the protection of plants from stress: clues from transgenic plants,” Plant Cell Environ., vol. 25, 163-171, 2002.
V.E Sharkova, “The effect of heat shock on the capacity of wheat plants to restore their photosynthetic electron transport after photoinhibition or repeated heating,” Russ J Plant Physiol, vol. 48, 793-97, 2001.
R. Mittler, “Oxidative stress, antioxidants and stress tolerance,” Trends Plant Sci., vol. 7, 405-410, 2002.
M. Almeselmani, P.S. Deshmukh, R.K. Sairam, S.R. Kushwaha, T.P. Singh, “Protective role of antioxidant enzymes under high temperature stress,” Plant Sci., vol.171, 382-388, 2006.
F.V. Breusegem, E.Vranova, J.F Dat, D. Inze, “The role of active oxygen species in plant signal transduction,” Plant Sci., vol. 161, 405-414, 2001.
B. Asthir, A. Koundal, N.S. Bains, “Kinetic and thermodynamic behavior of wall-bound peroxidase from wheat leaves infected with stripe rust,” Plant Growth Regul., vol. 59, 117-124, 2009.
Le Roy Holm, J. Doll, E. H. Juan V, Pancho, P. James, Harberger, World Weeds: Natural histories and distribution, John Wiley and Sons. 67-69, 1997.
S. Ignacimuthu, A. Jeyasankar N. Raja, “A process for the preparation of an active compound 2,5 diacetoxy-2-benzyl, 4,4,6,6-tetra methyl 1-1, 3-cyclohexanedione having insecticidal property, 579/CHE/2005, (Patent No. 236541),” Patentee: Entomology Research Institute., 2004.
T. Bhakta, P.K. Mukherjee, K. Saha, M. Pal, B.P. Saha, “Studies on in vivo wound healing activity of Cassia fistula L. leaves (Fam. Leguminosae) in rats,” Natural Product Sciences, vol. 4, 84-87, 1998.
K.R. Kirtikar, B.D. Basu, “Indian Medicinal Plants” International book distributors, vol. 2, 856-860, 2006.
J. Hutchinson, J.M. Dalziel, “Flora of West Tropical Africa, Second Edition, Vol. 1, Part 2. Crown Agents for Oversea Governments and Administrations, London. pp. 450-455, 1958.
R. Prasanna, C. Harish, R. Pichai, D. Sakthisekaran, P. Gunasekaran, “Anti-cancer effect of Cassia auriculata leaf extract in vitro through cell cycle arrest and induction of apoptosis in human breast and larynx cancer cell lines,” Cell Biol. Int., vol 33,127-134, 2009.
P. Manickam, N. Namasivayam, V. Periyasamy, S.K. Rajagopal, “Effect of Cassia auriculata leaf extract on lipids in rats with alcoholic liver injury,” Asia Pacific Journal of Clinical Nutrition., vol. 11, 157-163, 2002.
International Rules for Seed Testing. ISTA, Seed Science & Technology. vol.13, 299-335, 1985.
D.T Arnon, “Copper enzymes in isolated chloroplast polyphenol oxidase in Beta vulgaris,” Plant Physiol. vol. 24, 1-15, 1949.
O.H. Lowry, N.J. Rosebrough, A.L. Farr, R.J. Randall, “Protein measurement with Folin- Phenol reagent,” J. Biol. Chem. vol. 193, 265-275, 1951.
S. Ranganna, Handbook of analysis and quality control for fruit and vegetable products. 2nd Edn. Tata Mc Graw Hill Publication Co. ltd, New Delhi, 1986.
K.B. Kumar, P.A. Khan, “Peroxidase and polyphenol oxidase in excised Ragi (Eleusine coracana cv. PR 202) leaves during senescence,” Ind J Exp Bot., vol. 20, 412-416, 1982.
J.M. Chandlee, J.G. Scandalios, “Analysis of variants affecting the catalase development program in maize scutellum,” Theor Appl Genet. vol. 69, 71-77, 1984.
C. Beauchamp, I. Fridovich, Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Anal. Biochem. vol. 44, 276-287, 1971.
T. Pasternak, V. Rudas, G. Potters, M.A.K. Jansen, “Morphogenic effects of abiotic stress: reorientation of growth in Arabidopsis thaliana seedlings, Environ. Exp. Bot, vol 53, 299-314, 2005.
L.E. Williams, R. Lemonie, N. Saucer, Sugars transporters in higher plants: a diversity of roles and complex regulation trends, Plant Sci., vol. 5, 283-290, 2000.
E.P. Murakeozy, Z. Nagy, C. Duhaze, A. Bouchereau, Z. Tuba, Seasonal changes in the levels of compatible osmolytes in three halophytic species of inland saline vegetation in Hungary, J. Plant Physiol., vol. 160, 395-401, 2003.
H. Nayar, “Putrescine increases floral retention, pod set and seed yield in cold stressed chickpea,” J Agron Crop Sci., vol. 191, 340-345, 2005.
Z.Z. Xu, G.S Zhou, “Combined effects of water stress and high temperature on photosynthesis nitrogen metabolism and lipid peroxidation of a perennial grass Leymus chinensis,” Planta., vol. 24, 1080-1090, 2006.
M.T.P. Isabela, P.P.L Gluseppina, A. Henrique, O.G. Brasil, “Effect of pH on proline contents and peroxidise activity in metabolism of Sugarcane cultivated in vitro,” Agronomia Trop. vol.53, 145-146, 2003.
K. Lichtenthler, G. Langsdorf, S. Lenk, C. Buschamann, “Chlorophyll fluorescence imaging of photosynthetic activity with the flesh lamp fluorescence imaging system,” Photosynthetic, vol. 43, 355-369, 2005.
A. Gur, U. Demirel, M. Ozden, A. Kahraman, O. Copur, “Diurnal gradual heat stress affects antioxidant enzymes, proline accumulation and some physiological components in Cotton,” Afr. J. Biotechnol., vol 9, 1008-1015, 2010.
G. Noctor, C.H. Foyer, “Ascorbate and glutathione: keeping active oxygen under control,” Annu Rev of Plant Physiol Plant Mol Biol., vol. 49, 249-279, 1998.
R.K Sairam, A. Tyagi, “Physiological and molecular biology of salinity stress tolerance in plants,” Curr. Sci., vol. 86, 407–420, 2004.
J.G. Scandalios, Oxygen stress and superoxide dismutase, Plant Physiology., vol 101, 712-726, 1993.
E. Esfandiari, F. Shekari, F. Shekari, M. Esfandiari, “The effect of salt stress on antioxidant enzymes activity and lipid peroxidation on the wheat seedling,” Not. Bot. Hort. Agrobot. Cluj., vol. 35, 48-56, 2007.
R. Nagesh Babu, V.R. Devaraj, “High temperature and salt stress response in French bean (Phaseolus vulgaris),” Aus. J. Crop Sci., vol. 2, 40-48, 2008.
J.H. Dat, C.H. Foyer, I.M. Scott, “Changes in salicyclic acid and antioxidants during induced thermotolerance in mustard seedlings,” Plant Physiol., vol 88, 1455-1461, 1998.
S. Agarwal, V. Pandey, “Antioxidant enzyme response to NaCl stress in Cassia angustifolia,” Biol. Plantarum., vol. 48, 555-560, 2004.