Isolation, Cloning and Expression of Rhamnolipid Operon from Pseudomonas aeroginosa ATCC 9027 in Logarithmic Phase in E. coli BL21
American Journal of Life Sciences
Volume 2, Issue 6-3, December 2014, Pages: 22-30
Received: Oct. 19, 2014; Accepted: Dec. 8, 2014; Published: Mar. 23, 2017
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Authors
Amin Jafari, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran
Jamshid Raheb, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran
Hassan Bardania, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran
Behnam Rasekh, Department of Petroleum Biotechnology, Biotechnology Research Center, Research Institute of Petroleum Industry, Tehran, Iran
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Abstract
The rhamnolipid operon from Pesudomonas strain with the native promoter was not expressed in logarithmic phase of Ecoli. The expression of rhamnolipid in logarithmic phase of growth whether the regulatory elements of the operon are eliminated or not was investigated. The rhamnolipid operon was identified in Pseudomonas aeruginosa ATCC 9027 and the rhlAB genes related to rhamnolipid were isolated and amplified by PCR. The PCR product was cloned in pET 23a expression vector and transferred into the E. coli BL21. The expression of rhlAB genes was analyzed and our results showed that the synthesis of monorhamnolipid occurred in logarithmic phase. In addition this data demonstrated a higher production of rhamnolipid in recombinant Ecoli Bl21compared to that indigenous Pseudomonas aeruginosa ATCC 9027.
Keywords
MEOR, Manipulated E. coli BL21, Monorhamnolipid, Logarithmic Phase
To cite this article
Amin Jafari, Jamshid Raheb, Hassan Bardania, Behnam Rasekh, Isolation, Cloning and Expression of Rhamnolipid Operon from Pseudomonas aeroginosa ATCC 9027 in Logarithmic Phase in E. coli BL21, American Journal of Life Sciences. Special Issue: Microbiology Research. Vol. 2, No. 6-3, 2014, pp. 22-30. doi: 10.11648/j.ajls.s.2014020603.15
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