Recognition of Acute Toxoplasmosis with IgG Avidity ELISA Test in the Pregnant Women (The First Trimester) in Qom Province, Iran, during Two Years (2012-2013)

Mohammad Reza Haeri; Behnaz Jalalizadegan; Fatemeh Tabatabaie

doi:doi:10.11648/j.ajls.s.2014020603.14

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Recognition of Acute Toxoplasmosis with IgG Avidity ELISA Test in the Pregnant Women (The First Trimester) in Qom Province, Iran, during Two Years (2012-2013)

Mohammad Reza Haeri, Behnaz Jalalizadegan, Fatemeh Tabatabaie

Published in American Journal of Life Sciences (Volume 2, Issue 6-3)

Received: 5 December 2014 Accepted: 12 December 2014 Published: 27 December 2014

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Abstract

Toxoplasma gondii is an obligate intracellular protozoan parasite occurring with a global distribution. Congenital toxoplasmosis results when a non-immune woman gets a primary infection during pregnancy. Chronically infected women is not transmitted the infection to their offspring. The aim of this study was to perform IgG avidity test for detection of acute toxoplasmosis in the pregnant women (The first trimester). A total of 200 human serum samples were collected from different laboratories in Qom province. The anti-toxoplasma IgG, IgM and IgG avidity were evaluated by ELISA method. The samples were divided into 2 segregated groups: Group I consisted of 25 serum samples from patients in acute phase of Toxoplasma infection. All the pa¬tients had the symbol of lymphadenopathy. Group II consisted of 185 serum samples prepared from pa-tients in chronic phase of Toxoplasma infection. The data were analyzed by descriptive statistical methods. 20 out of 25 (80%) sera with acute toxoplasmosis showed low avidity levels and 178 out of 185 (96%) sera in chronic phase of infection showed high avidity index. 7sera had borderline ranges of AI. In this study, IgM ELISA and IgG avidity ELISA tests together could confirm the acute and chronic phases of disease completely. The IgG avidity test was created to help distinguish between past and recently acquired infection.

Published in	American Journal of Life Sciences (Volume 2, Issue 6-3) This article belongs to the Special Issue Microbiology Research
DOI	10.11648/j.ajls.s.2014020603.14
Page(s)	18-21
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Toxoplasmosis, IgG Avidity Test, ELISA, IgM

References

[1]	Jenum PA, Stray-Pedersen B, Gundersen AG., Improved diagnosis of primary Toxoplasma gondii infection in early pregnancy by determination of antitoxoplasma immunoglobulin G avidity. J Clin Microbiol. 1997; 35:1972-7. PubMed Central PMCID: PMC229886
[2]	Joynson DH, Payne RA, Rawal BK., Potential role of IgG avidity for diagnosing Toxoplasmosis. J Clin Pathol. 1990; 43: 1032-3. PubMed Central PMCID: PMC502981
[3]	Yasodhara P, Ramalakshmi BA, Sarma MK., A new approach to differentiate recent vs chronic Toxoplasma infection:avidity ELISA in Toxoplasma serology. Indian J Med Microbiol. 2001; 19:145-8. PubMed PMID:17664818
[4]	Roberts A, Hedman K, Luyasu V, Zufferey J, Bessières MH, Blatz RM et al ., Multicenter evaluation of strategies for serodi¬agnosis of primary infection with Toxoplasma gondii. Eur J Clin Microbiol Infect Dis; 2001, 20: 467-474. PubMed PMID:11561802
[5]	Crucerescu E, Lovin DR., Study on specific IgG avidity as a tool for recent primary Toxoplasma gondii infection diagnosis. J Prev Med; 2002, 10 (3): 56-62.
[6]	Marcolino PT, Silva DAO, Leser PG, Camargo ME, Mineo JR., Molecular markers in acute and chronic phases of human toxo¬plasmosis: determination of immunoglobulin G avidity by West¬ern blotting. Clin Diagn Lab Immunol; 2000,7: 384-389, PubMed Central PMCID: PMC95883
[7]	Rahbari A.H, Keshavarz H, Shojaee S, Mohebali M and Rezaeian M., IgG Avidity ELISA Test for Diagnosis of Acute Toxoplasmosis in Humans, Korean J Parasitol , 2012,50(2): 99-102. PubMed Central PMCID: PMC3375462
[8]	Hedman K, Lappalainen M, Seppaia I, Makela O., Recent primary toxoplasma infection indicated by a low avidity of specific IgG. J Infect Dis; 1989, 159: 736-740. PubMed PMID:2926163
[9]	Horvjth KN, Szénjsi Z, Danka J, Kucsera I., Value of the IgG avidity in the diagnosis of recent toxoplasmosis: A comparative study of four commercially available anti-Toxoplasma gondii IgG avidity assays. Acta Parasitologica.; 2005,50:255-60.
[10]	Tanyuksel M, Guney C, Araz E, Saracli MA, Doganci L., Performance of the Immunoglobulin G Avidity and Enzyme Immunoassay IgG/IgM Screening Tests for Differentiation of the Clinical Spectrum of toxoplasmosis. J Microbiol.; 2004,42:211-5. PubMed PMID:15459650
[11]	Candolfi E, Pastor R, Huber R, Filisetti D, Villard O., IgG avidity assay firms up the diagnosis of acute toxoplasmosis on the first serum sample in immunocompetent pregnant women. Diagn Microbiol Infect Dis.; 2007, 58: 83-8. PubMed PMID: 17368807
[12]	Hedman K, Seppala I., Recent rubella virus infection indicated by a low avidity of specific IgG. J Clin Immunol; 1988,8: 214-221. PubMed PMID:3292566
[13]	Remington JS, Thulliez P, Montoya JG., Recent developments for diagnosis of toxoplasmosis. J Clin Microbiol; 2004,42: 941-945. doi: 10.1128/JCM.42.3.941-945.2004,PubMed Central PMCID: PMC356902
[14]	Mechain B, Garin YJ, Robert-Gangneux F, Dupouy-Camet J, Derouin F., Lack of utility of specific immunoglobulin G antibody avidity for serodiagnosis of reactivated toxoplasmosis in immunocompromised patients. Clin Diagn Lab Immunol; 2000,7: 703-705. PubMed PMID:10882677 , PubMed CentralPMCID:PMC95939
[15]	Pour Abolghasem Sh, Bonyadi M .R, Babaloo Z, Porhasan A, Nagili B, Gardashkhani O.A et al ., IgG Avidity Test for the Diagnosis of Acute Toxoplasma gondii Infection in Early Pregnancy ,Iran. J. Immunol. December, 2011,8 (4), 251-255, doi: IJIv8i4A8, PubMed PMID:22201623
[16]	Liesenfeld O, Montoya JG, Kinney S, Press C and RemingtonJS., Effect of Testing for IgG Avidity in the Diagnosis oToxoplasmagondii Infection in Pregnant Women: Experience in aUS Reference Laboratory. J Infect Dis, 2001; 183: 1248-1253.
[17]	Tlamçani Z, Lemkhenete Z, Lmimouni E. B. ,Toxoplasmosis: The value of molecular methods in diagnosis compared to conventional methods Journal of Microbiology and Infectious Diseases, 2013; 3 (2): 93-99

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Mohammad Reza Haeri, Behnaz Jalalizadegan, Fatemeh Tabatabaie. (2014). Recognition of Acute Toxoplasmosis with IgG Avidity ELISA Test in the Pregnant Women (The First Trimester) in Qom Province, Iran, during Two Years (2012-2013). American Journal of Life Sciences, 2(6-3), 18-21. https://doi.org/10.11648/j.ajls.s.2014020603.14

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ACS Style

Mohammad Reza Haeri; Behnaz Jalalizadegan; Fatemeh Tabatabaie. Recognition of Acute Toxoplasmosis with IgG Avidity ELISA Test in the Pregnant Women (The First Trimester) in Qom Province, Iran, during Two Years (2012-2013). Am. J. Life Sci. 2014, 2(6-3), 18-21. doi: 10.11648/j.ajls.s.2014020603.14

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AMA Style

Mohammad Reza Haeri, Behnaz Jalalizadegan, Fatemeh Tabatabaie. Recognition of Acute Toxoplasmosis with IgG Avidity ELISA Test in the Pregnant Women (The First Trimester) in Qom Province, Iran, during Two Years (2012-2013). Am J Life Sci. 2014;2(6-3):18-21. doi: 10.11648/j.ajls.s.2014020603.14

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@article{10.11648/j.ajls.s.2014020603.14,
  author = {Mohammad Reza Haeri and Behnaz Jalalizadegan and Fatemeh Tabatabaie},
  title = {Recognition of Acute Toxoplasmosis with IgG Avidity ELISA Test in the Pregnant Women (The First Trimester) in Qom Province, Iran, during Two Years (2012-2013)},
  journal = {American Journal of Life Sciences},
  volume = {2},
  number = {6-3},
  pages = {18-21},
  doi = {10.11648/j.ajls.s.2014020603.14},
  url = {https://doi.org/10.11648/j.ajls.s.2014020603.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajls.s.2014020603.14},
  abstract = {Toxoplasma gondii is an obligate intracellular protozoan parasite occurring with a global distribution. Congenital toxoplasmosis results when a non-immune woman gets a primary infection during pregnancy. Chronically infected women is not transmitted the infection to their offspring. The aim of this study was to perform IgG avidity test for detection of acute toxoplasmosis in the pregnant women (The first trimester). A total of 200 human serum samples were collected from different laboratories in Qom province. The anti-toxoplasma IgG, IgM and IgG avidity were evaluated by ELISA method. The samples were divided into 2 segregated groups: Group I consisted of 25 serum samples from patients in acute phase of Toxoplasma infection. All the pa¬tients had the symbol of lymphadenopathy. Group II consisted of 185 serum samples prepared from pa-tients in chronic phase of Toxoplasma infection. The data were analyzed by descriptive statistical methods. 20 out of 25 (80%) sera with acute toxoplasmosis showed low avidity levels and 178 out of 185 (96%) sera in chronic phase of infection showed high avidity index. 7sera had borderline ranges of AI. In this study, IgM ELISA and IgG avidity ELISA tests together could confirm the acute and chronic phases of disease completely. The IgG avidity test was created to help distinguish between past and recently acquired infection.},
 year = {2014}
}

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TY - JOUR
T1 - Recognition of Acute Toxoplasmosis with IgG Avidity ELISA Test in the Pregnant Women (The First Trimester) in Qom Province, Iran, during Two Years (2012-2013)
AU - Mohammad Reza Haeri
AU - Behnaz Jalalizadegan
AU - Fatemeh Tabatabaie
Y1 - 2014/12/27
PY - 2014
N1 - https://doi.org/10.11648/j.ajls.s.2014020603.14
DO - 10.11648/j.ajls.s.2014020603.14
T2 - American Journal of Life Sciences
JF - American Journal of Life Sciences
JO - American Journal of Life Sciences
SP - 18
EP - 21
PB - Science Publishing Group
SN - 2328-5737
UR - https://doi.org/10.11648/j.ajls.s.2014020603.14
AB - Toxoplasma gondii is an obligate intracellular protozoan parasite occurring with a global distribution. Congenital toxoplasmosis results when a non-immune woman gets a primary infection during pregnancy. Chronically infected women is not transmitted the infection to their offspring. The aim of this study was to perform IgG avidity test for detection of acute toxoplasmosis in the pregnant women (The first trimester). A total of 200 human serum samples were collected from different laboratories in Qom province. The anti-toxoplasma IgG, IgM and IgG avidity were evaluated by ELISA method. The samples were divided into 2 segregated groups: Group I consisted of 25 serum samples from patients in acute phase of Toxoplasma infection. All the pa¬tients had the symbol of lymphadenopathy. Group II consisted of 185 serum samples prepared from pa-tients in chronic phase of Toxoplasma infection. The data were analyzed by descriptive statistical methods. 20 out of 25 (80%) sera with acute toxoplasmosis showed low avidity levels and 178 out of 185 (96%) sera in chronic phase of infection showed high avidity index. 7sera had borderline ranges of AI. In this study, IgM ELISA and IgG avidity ELISA tests together could confirm the acute and chronic phases of disease completely. The IgG avidity test was created to help distinguish between past and recently acquired infection.
VL - 2
IS - 6-3
ER -

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Author Information

Mohammad Reza Haeri

School of medicine, Qom University of medical sciences, Qom, Ira
Behnaz Jalalizadegan

Department of Parasitology and Mycology, School of Medicine, Ira
Fatemeh Tabatabaie

Department of Parasitology and Mycology, School of Medicine, Ira

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Plain Text BibTeX RIS

APA Style

Mohammad Reza Haeri, Behnaz Jalalizadegan, Fatemeh Tabatabaie. (2014). Recognition of Acute Toxoplasmosis with IgG Avidity ELISA Test in the Pregnant Women (The First Trimester) in Qom Province, Iran, during Two Years (2012-2013). American Journal of Life Sciences, 2(6-3), 18-21. https://doi.org/10.11648/j.ajls.s.2014020603.14

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ACS Style

Mohammad Reza Haeri; Behnaz Jalalizadegan; Fatemeh Tabatabaie. Recognition of Acute Toxoplasmosis with IgG Avidity ELISA Test in the Pregnant Women (The First Trimester) in Qom Province, Iran, during Two Years (2012-2013). Am. J. Life Sci. 2014, 2(6-3), 18-21. doi: 10.11648/j.ajls.s.2014020603.14

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AMA Style

Mohammad Reza Haeri, Behnaz Jalalizadegan, Fatemeh Tabatabaie. Recognition of Acute Toxoplasmosis with IgG Avidity ELISA Test in the Pregnant Women (The First Trimester) in Qom Province, Iran, during Two Years (2012-2013). Am J Life Sci. 2014;2(6-3):18-21. doi: 10.11648/j.ajls.s.2014020603.14

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@article{10.11648/j.ajls.s.2014020603.14,
  author = {Mohammad Reza Haeri and Behnaz Jalalizadegan and Fatemeh Tabatabaie},
  title = {Recognition of Acute Toxoplasmosis with IgG Avidity ELISA Test in the Pregnant Women (The First Trimester) in Qom Province, Iran, during Two Years (2012-2013)},
  journal = {American Journal of Life Sciences},
  volume = {2},
  number = {6-3},
  pages = {18-21},
  doi = {10.11648/j.ajls.s.2014020603.14},
  url = {https://doi.org/10.11648/j.ajls.s.2014020603.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajls.s.2014020603.14},
  abstract = {Toxoplasma gondii is an obligate intracellular protozoan parasite occurring with a global distribution. Congenital toxoplasmosis results when a non-immune woman gets a primary infection during pregnancy. Chronically infected women is not transmitted the infection to their offspring. The aim of this study was to perform IgG avidity test for detection of acute toxoplasmosis in the pregnant women (The first trimester). A total of 200 human serum samples were collected from different laboratories in Qom province. The anti-toxoplasma IgG, IgM and IgG avidity were evaluated by ELISA method. The samples were divided into 2 segregated groups: Group I consisted of 25 serum samples from patients in acute phase of Toxoplasma infection. All the pa¬tients had the symbol of lymphadenopathy. Group II consisted of 185 serum samples prepared from pa-tients in chronic phase of Toxoplasma infection. The data were analyzed by descriptive statistical methods. 20 out of 25 (80%) sera with acute toxoplasmosis showed low avidity levels and 178 out of 185 (96%) sera in chronic phase of infection showed high avidity index. 7sera had borderline ranges of AI. In this study, IgM ELISA and IgG avidity ELISA tests together could confirm the acute and chronic phases of disease completely. The IgG avidity test was created to help distinguish between past and recently acquired infection.},
 year = {2014}
}

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