American Journal of Life Sciences

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Effect of Volatile and Non-Volatile Compounds of Trichoderma spp. on Botrytis Fabae the Causative Agent of Faba Bean Chocolate Spot

Received: 20 August 2014    Accepted: 25 August 2014    Published: 05 September 2014
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Abstract

Antagonistic fungi naturally occurring on faba bean leaf surface were isolated and evaluated for their activity as bioagents for Botrytis fabae the causative agent of chocolate spot disease. Thirty isolates were purified and identified as 26 isolates of Trichoderma species (Trichoderma album, T. aureoviride , T. hamatum, T. harzianum and T. viride) and 4 isolates belonging to the genera of Cladosporium, Gliocladium, Epicoccum and Paecilomyces. The inhibitory effect of these isolates was assessed in vitro against the growth of B. fabae, which decreased it's mycelial growth on PDA plates. The inhibitory effect of Trichoderma spp. ranged between 51.11 - 77.78%. In addition, T. album (Isolate 2) gave the highest inhibition followed by T. harzianum (Isolate 6). Furthermore, under greenhouse conditions spraying of faba bean plants with any of Trichoderma spp. and Bio- Zeid as a biofungicide, 24 h before inoculation with B. fabae significantly reduced the severity of the disease after 14 days in the range of 3.0 - 4% compared with the control (8.7%). T. album (Isolate 2) was the highest antagonistic isolate (3.0%) followed by T. harzianum (Isolate 6) then T. hamatum (Isolate 6) and T. viride (Isolate 2), being 3.24, 3.30 and 3.40%, respectively. Volatile and non-volatile compounds produced by T. album (Isolate 2) exhibited the highest inhibition to the mycelial growth of B. fabae followed by T. harzianum (Isolate 6).

DOI 10.11648/j.ajls.s.2014020602.12
Published in American Journal of Life Sciences (Volume 2, Issue 6-2, December 2014)

This article belongs to the Special Issue Role of Combination Between Bioagents and Solarization on Management of Crown-and Stem-Rot of Egyptian Clover

Page(s) 11-18
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Biological Control, Botrytis Fabae, Faba Bean Trichoderma Spp, Volatile and Non-Volatile Compounds

References
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Author Information
  • Plant Pathology Department, Faculty of Agriculture, Cairo University, Egypt

  • Plant Pathology Department, Faculty of Agriculture, Cairo University, Egypt

  • Plant Pathology Research Institute, ARC. Giza, Egypt

  • Plant Pathology Research Institute, ARC. Giza, Egypt

Cite This Article
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    Barakat F. M., Abada K. A., Abou-Zeid N. M., El-Gammal Y. H. E. (2014). Effect of Volatile and Non-Volatile Compounds of Trichoderma spp. on Botrytis Fabae the Causative Agent of Faba Bean Chocolate Spot. American Journal of Life Sciences, 2(6-2), 11-18. https://doi.org/10.11648/j.ajls.s.2014020602.12

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    Barakat F. M.; Abada K. A.; Abou-Zeid N. M.; El-Gammal Y. H. E. Effect of Volatile and Non-Volatile Compounds of Trichoderma spp. on Botrytis Fabae the Causative Agent of Faba Bean Chocolate Spot. Am. J. Life Sci. 2014, 2(6-2), 11-18. doi: 10.11648/j.ajls.s.2014020602.12

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    AMA Style

    Barakat F. M., Abada K. A., Abou-Zeid N. M., El-Gammal Y. H. E. Effect of Volatile and Non-Volatile Compounds of Trichoderma spp. on Botrytis Fabae the Causative Agent of Faba Bean Chocolate Spot. Am J Life Sci. 2014;2(6-2):11-18. doi: 10.11648/j.ajls.s.2014020602.12

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  • @article{10.11648/j.ajls.s.2014020602.12,
      author = {Barakat F. M. and Abada K. A. and Abou-Zeid N. M. and El-Gammal Y. H. E.},
      title = {Effect of Volatile and Non-Volatile Compounds of Trichoderma spp. on Botrytis Fabae the Causative Agent of Faba Bean Chocolate Spot},
      journal = {American Journal of Life Sciences},
      volume = {2},
      number = {6-2},
      pages = {11-18},
      doi = {10.11648/j.ajls.s.2014020602.12},
      url = {https://doi.org/10.11648/j.ajls.s.2014020602.12},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.ajls.s.2014020602.12},
      abstract = {Antagonistic fungi naturally occurring on faba bean leaf surface were isolated and evaluated for their activity as bioagents for Botrytis fabae the causative agent of chocolate spot disease. Thirty isolates were purified and identified as 26 isolates of Trichoderma species (Trichoderma album, T. aureoviride , T. hamatum, T. harzianum and T. viride) and 4 isolates belonging to the genera of Cladosporium, Gliocladium, Epicoccum and Paecilomyces. The inhibitory effect of these isolates was assessed in vitro against the growth of B. fabae, which decreased it's mycelial growth on PDA plates. The inhibitory effect of Trichoderma spp. ranged between 51.11 - 77.78%. In addition, T. album (Isolate 2) gave the highest inhibition followed by T. harzianum (Isolate 6). Furthermore, under greenhouse conditions spraying of faba bean plants with any of Trichoderma spp. and Bio- Zeid as a biofungicide, 24 h before inoculation with B. fabae significantly reduced the severity of the disease after 14 days in the range of 3.0 - 4% compared with the control (8.7%). T. album (Isolate 2) was the highest antagonistic isolate (3.0%) followed by T. harzianum (Isolate 6) then T. hamatum (Isolate 6) and T. viride (Isolate 2), being 3.24, 3.30 and 3.40%, respectively. Volatile and non-volatile compounds produced by T. album (Isolate 2) exhibited the highest inhibition to the mycelial growth of B. fabae followed by T. harzianum (Isolate 6).},
     year = {2014}
    }
    

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    T1  - Effect of Volatile and Non-Volatile Compounds of Trichoderma spp. on Botrytis Fabae the Causative Agent of Faba Bean Chocolate Spot
    AU  - Barakat F. M.
    AU  - Abada K. A.
    AU  - Abou-Zeid N. M.
    AU  - El-Gammal Y. H. E.
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    T2  - American Journal of Life Sciences
    JF  - American Journal of Life Sciences
    JO  - American Journal of Life Sciences
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    EP  - 18
    PB  - Science Publishing Group
    SN  - 2328-5737
    UR  - https://doi.org/10.11648/j.ajls.s.2014020602.12
    AB  - Antagonistic fungi naturally occurring on faba bean leaf surface were isolated and evaluated for their activity as bioagents for Botrytis fabae the causative agent of chocolate spot disease. Thirty isolates were purified and identified as 26 isolates of Trichoderma species (Trichoderma album, T. aureoviride , T. hamatum, T. harzianum and T. viride) and 4 isolates belonging to the genera of Cladosporium, Gliocladium, Epicoccum and Paecilomyces. The inhibitory effect of these isolates was assessed in vitro against the growth of B. fabae, which decreased it's mycelial growth on PDA plates. The inhibitory effect of Trichoderma spp. ranged between 51.11 - 77.78%. In addition, T. album (Isolate 2) gave the highest inhibition followed by T. harzianum (Isolate 6). Furthermore, under greenhouse conditions spraying of faba bean plants with any of Trichoderma spp. and Bio- Zeid as a biofungicide, 24 h before inoculation with B. fabae significantly reduced the severity of the disease after 14 days in the range of 3.0 - 4% compared with the control (8.7%). T. album (Isolate 2) was the highest antagonistic isolate (3.0%) followed by T. harzianum (Isolate 6) then T. hamatum (Isolate 6) and T. viride (Isolate 2), being 3.24, 3.30 and 3.40%, respectively. Volatile and non-volatile compounds produced by T. album (Isolate 2) exhibited the highest inhibition to the mycelial growth of B. fabae followed by T. harzianum (Isolate 6).
    VL  - 2
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