American Journal of Life Sciences

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Antagonistic Activity and Biofilm Studies on Streptomyces Species towards Nigrospora Sp

Received: 19 August 2014    Accepted: 10 September 2014    Published: 15 October 2014
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Abstract

Plants, in contrast with vertebrates, have no immune system. So they are affected by pathogens easily leading to remarkable yield loss altogether. Fungal phytopathogens pose serious problems worldwide in the agriculture sector, and harmful and costly chemical fungicides are nowhere a match to them. A detailed study was performed to screen the cooperative role of Streptomyces coelicolor and Streptomyces halstedii towards the fungal pathogen Nigrospora, a predominant pathogen of banana plantation. Many works were published stating the antagonistic activity of the Streptomyces species. For the first time we tried to study against the Nigrospora, which is a predominant pathogen of banana plant. Moreover we tried to find out the cooperative role of both the species towards the fungal pathogen. Biofilm formation studies were done using the ethanol method, and Chitinase activity was quantified using DNS method. Free radical scavenging activity was quantified using the standard DPPH method. The antioxidant profiles were screened using the TLC plate in mobile phase (5:4:3 (v/v/v) n-butanol/Methanol/16% aqueous ammonia). The antagonistic screening test done using the cup plate method proved the cooperative role of both the species. The activity of chitinase was observed for all the groups. Both the species showed chitinase activity, but when they are co cultured the activity was found to be enhanced. Even the co culture study also proved of the strong biofilm formation. Previous literature also showed of the exhibition of biofilm formation of the Streptomyces species. The increase or stability in the values proves of the possible cooperative role of both the species in the antagonistic activity against the Nigrospora species. A one way ANOVA was done to show the significance in the formation of biofilms. Peculiar results were obtained in the TLC. The Rf values obtained were compared with the standard antioxidant Rf values. The Rf values of 0.12 match with the catechin, and 0.4 match with the Quercetin, 06 match with Caffeic acid.

DOI 10.11648/j.ajls.s.2014020601.16
Published in American Journal of Life Sciences (Volume 2, Issue 6-1, November 2014)

This article belongs to the Special Issue Recent Developments in Health Care through Plants and Microbes

Page(s) 28-34
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Stretomyces Coelicolor, Streptmyces Halstedii, Antifungal Antagonistic Activity, Nigrospora, TLC

References
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[2] AL Garda, JMF Abalos, P Sanchez, A R Arribas, RI Santamaria, 1997, Two genes encoding an endoglucanase and a cellulose-binding protein are clustered and co-regulated by a TTA codon in Streptomyces halstedii JM8. Biochem J 324:403–411.
[3] G Duan, N Christian, J Schwachtje, D Walther, and O Ebenhöh, 2013. The Metabolic Interplay between Plants and Phytopathogens, Metabolites, 3, 1-23.
[4] G Vaaje-Kolstad, S J Horn, M. F Daan, B Synstad, and Vincent G. H. Eijsink 2005, The Non-catalytic Chitin-binding Protein CBP21 from Serratia marcescens Is Essential for Chitin Degradation J. Biol. Chem. 2005, 280:28492-28497.
[5] B Nazari, M Kobayashi, A Saito, A Hassaninasa, K Miyashita, T Fujiia 2013, Chitin-Induced Gene Expression in Secondary Metabolic Pathways of Streptomyces coelicolor A3(2) Grown in Soil. Applied and Environmental Microbiology p. Volume 79, 707–713.
[6] B Prapagdee, C Kuekulvong and S Mongkolsuk, 2008, Antifungal Potential of Extracellular Metabolites Produced by Streptomyces hygroscopicus against Phytopathogenic Fungi, Int. J. Biol. Sci., 4. 330-337.
[7] B. B. Aam, E. B. Heggset , A. L Norberg, M. S. Kjell, M. Varum and V. G. H. Eijsink, 2010, Production of Chitooligosaccharides and their potential Applications in Medicine, Mar. Drugs, 8, 1482-1517
[8] C Shi, P Yan, J Li, H Wu, Q Li and S Guan 2014. Biocontrol of Fusarium graminearum Growth and Deoxynivalenol Production in Wheat Kernels with Bacterial Antagonists, Int. J. Environ. Res. Public Health, 11, 1094-1105.
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[11] K.C Hoang, T. H Lai, C. S Lin, Y. T Chen and C. Y Liau, 2011. The Chitinolytic Activities of Streptomyces sp. TH-11, Int. J. Mol. Sci., 12, 56-65.
[12] A. S. Magdalena E. Tenconi,b S. Rigali, and P. Gilles. VWezela 2011. Functional Analysis of the N-Acetylglucosamine Metabolic Genes of Streptomyces coelicolor and Role in Control of Development and Antibiotic Production. Journal of Bacteriology p. 1136–1144.
[13] M. S Brzezinsk U. Jankiewicz., 2012, Production of Antifungal Chitinase by Aspergillus niger LOCK 62 and Its Potential Role in the Biological Control, Curr Microbiol 65:666–672.
[14] R. Nandakumar, S. Babu1, T. Raguchander and R. Samiyappan, 2007, Chitinolytic Activity of Native Pseudomonas fluorescens Strains J. Agric. Sci. Technol. Vol. 9: 61-68.
[15] T. Kawase, S. Yokokawa, A. Saito; T. Fujii, N. nikaidou, K. Miyashita, and Takeshi Watanabe, 2005. Comparison of Enzymatic and Antifungal Properties between Family 18 and 19 Chitinases from S. coelicolor A3(2), Biosci. Biotechnol. Biochem., 70 (4), 988–998, 2006.
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[18] S. H Ji, N. C Paul, J. X Deng, Y. S Kim, B. S Yun and S. H Yu, 2013. Biocontrol Activity of Bacillus amyloliquefaciens CNU114001 against Fungal Plant Diseases, Mycobiology December, 41(4): 234-242.
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[20] T. Watanabe, W. Oyanagi, K. Suzuki, and H. Tanaka, 1990. Chitinase System of Bacillus circulans WL-12 and Importance of Chitinase Al in Chitin Degradation, J. Bacteriol. vol. 172 no. 74017-4022.
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Author Information
  • Indian Academy Degree College, Centre for Research & Post Graduate Studies, Bangalore, India

  • Indian Academy Degree College, Centre for Research & Post Graduate Studies, Bangalore, India

  • Indian Academy Degree College, Centre for Research & Post Graduate Studies, Bangalore, India

  • Indian Academy Degree College, Centre for Research & Post Graduate Studies, Bangalore, India

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    Sudhakar Malla, Bathula Christopher, Yacharam shivakumar, Kakarapalli Nagalakshmi. (2014). Antagonistic Activity and Biofilm Studies on Streptomyces Species towards Nigrospora Sp. American Journal of Life Sciences, 2(6-1), 28-34. https://doi.org/10.11648/j.ajls.s.2014020601.16

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    ACS Style

    Sudhakar Malla; Bathula Christopher; Yacharam shivakumar; Kakarapalli Nagalakshmi. Antagonistic Activity and Biofilm Studies on Streptomyces Species towards Nigrospora Sp. Am. J. Life Sci. 2014, 2(6-1), 28-34. doi: 10.11648/j.ajls.s.2014020601.16

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    AMA Style

    Sudhakar Malla, Bathula Christopher, Yacharam shivakumar, Kakarapalli Nagalakshmi. Antagonistic Activity and Biofilm Studies on Streptomyces Species towards Nigrospora Sp. Am J Life Sci. 2014;2(6-1):28-34. doi: 10.11648/j.ajls.s.2014020601.16

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  • @article{10.11648/j.ajls.s.2014020601.16,
      author = {Sudhakar Malla and Bathula Christopher and Yacharam shivakumar and Kakarapalli Nagalakshmi},
      title = {Antagonistic Activity and Biofilm Studies on Streptomyces Species towards Nigrospora Sp},
      journal = {American Journal of Life Sciences},
      volume = {2},
      number = {6-1},
      pages = {28-34},
      doi = {10.11648/j.ajls.s.2014020601.16},
      url = {https://doi.org/10.11648/j.ajls.s.2014020601.16},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.ajls.s.2014020601.16},
      abstract = {Plants, in contrast with vertebrates, have no immune system. So they are affected by  pathogens easily leading to remarkable yield loss altogether. Fungal phytopathogens pose serious problems worldwide in the agriculture sector, and harmful and costly chemical fungicides are nowhere a match to them. A detailed study was performed to screen the cooperative role of Streptomyces coelicolor and Streptomyces halstedii towards the fungal pathogen Nigrospora, a predominant pathogen of banana plantation. Many works were published stating the antagonistic activity of the Streptomyces species. For the first time we tried to study against the Nigrospora, which is a predominant pathogen of banana plant. Moreover we tried to find out the cooperative role of both the species towards the fungal pathogen. Biofilm formation studies were done using the ethanol method, and Chitinase activity was quantified using DNS method. Free radical scavenging activity was quantified using the standard DPPH method. The antioxidant profiles were screened using the TLC plate in mobile phase (5:4:3 (v/v/v) n-butanol/Methanol/16% aqueous ammonia). The antagonistic screening test done using the cup plate method proved the cooperative role of both the species. The activity of chitinase was observed for all the groups. Both the species showed chitinase activity, but when they are co cultured the activity was found to be enhanced. Even the co culture study also proved of the strong biofilm formation. Previous literature also showed of the exhibition of biofilm formation of the Streptomyces species. The increase or stability in the values proves of the possible cooperative role of both the species in the antagonistic activity against the Nigrospora species. A one way ANOVA was done to show the significance in the formation of biofilms. Peculiar results were obtained in the TLC. The Rf values obtained were compared with the standard antioxidant Rf values. The Rf values of 0.12 match with the catechin, and 0.4 match with the Quercetin, 06 match with Caffeic acid.},
     year = {2014}
    }
    

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  • TY  - JOUR
    T1  - Antagonistic Activity and Biofilm Studies on Streptomyces Species towards Nigrospora Sp
    AU  - Sudhakar Malla
    AU  - Bathula Christopher
    AU  - Yacharam shivakumar
    AU  - Kakarapalli Nagalakshmi
    Y1  - 2014/10/15
    PY  - 2014
    N1  - https://doi.org/10.11648/j.ajls.s.2014020601.16
    DO  - 10.11648/j.ajls.s.2014020601.16
    T2  - American Journal of Life Sciences
    JF  - American Journal of Life Sciences
    JO  - American Journal of Life Sciences
    SP  - 28
    EP  - 34
    PB  - Science Publishing Group
    SN  - 2328-5737
    UR  - https://doi.org/10.11648/j.ajls.s.2014020601.16
    AB  - Plants, in contrast with vertebrates, have no immune system. So they are affected by  pathogens easily leading to remarkable yield loss altogether. Fungal phytopathogens pose serious problems worldwide in the agriculture sector, and harmful and costly chemical fungicides are nowhere a match to them. A detailed study was performed to screen the cooperative role of Streptomyces coelicolor and Streptomyces halstedii towards the fungal pathogen Nigrospora, a predominant pathogen of banana plantation. Many works were published stating the antagonistic activity of the Streptomyces species. For the first time we tried to study against the Nigrospora, which is a predominant pathogen of banana plant. Moreover we tried to find out the cooperative role of both the species towards the fungal pathogen. Biofilm formation studies were done using the ethanol method, and Chitinase activity was quantified using DNS method. Free radical scavenging activity was quantified using the standard DPPH method. The antioxidant profiles were screened using the TLC plate in mobile phase (5:4:3 (v/v/v) n-butanol/Methanol/16% aqueous ammonia). The antagonistic screening test done using the cup plate method proved the cooperative role of both the species. The activity of chitinase was observed for all the groups. Both the species showed chitinase activity, but when they are co cultured the activity was found to be enhanced. Even the co culture study also proved of the strong biofilm formation. Previous literature also showed of the exhibition of biofilm formation of the Streptomyces species. The increase or stability in the values proves of the possible cooperative role of both the species in the antagonistic activity against the Nigrospora species. A one way ANOVA was done to show the significance in the formation of biofilms. Peculiar results were obtained in the TLC. The Rf values obtained were compared with the standard antioxidant Rf values. The Rf values of 0.12 match with the catechin, and 0.4 match with the Quercetin, 06 match with Caffeic acid.
    VL  - 2
    IS  - 6-1
    ER  - 

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