Antagonistic Activity and Biofilm Studies on Streptomyces Species towards Nigrospora Sp
American Journal of Life Sciences
Volume 2, Issue 6-1, November 2014, Pages: 28-34
Received: Aug. 19, 2014;
Accepted: Sep. 10, 2014;
Published: Oct. 15, 2014
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Sudhakar Malla, Indian Academy Degree College, Centre for Research & Post Graduate Studies, Bangalore, India
Bathula Christopher, Indian Academy Degree College, Centre for Research & Post Graduate Studies, Bangalore, India
Yacharam shivakumar, Indian Academy Degree College, Centre for Research & Post Graduate Studies, Bangalore, India
Kakarapalli Nagalakshmi, Indian Academy Degree College, Centre for Research & Post Graduate Studies, Bangalore, India
Plants, in contrast with vertebrates, have no immune system. So they are affected by pathogens easily leading to remarkable yield loss altogether. Fungal phytopathogens pose serious problems worldwide in the agriculture sector, and harmful and costly chemical fungicides are nowhere a match to them. A detailed study was performed to screen the cooperative role of Streptomyces coelicolor and Streptomyces halstedii towards the fungal pathogen Nigrospora, a predominant pathogen of banana plantation. Many works were published stating the antagonistic activity of the Streptomyces species. For the first time we tried to study against the Nigrospora, which is a predominant pathogen of banana plant. Moreover we tried to find out the cooperative role of both the species towards the fungal pathogen. Biofilm formation studies were done using the ethanol method, and Chitinase activity was quantified using DNS method. Free radical scavenging activity was quantified using the standard DPPH method. The antioxidant profiles were screened using the TLC plate in mobile phase (5:4:3 (v/v/v) n-butanol/Methanol/16% aqueous ammonia). The antagonistic screening test done using the cup plate method proved the cooperative role of both the species. The activity of chitinase was observed for all the groups. Both the species showed chitinase activity, but when they are co cultured the activity was found to be enhanced. Even the co culture study also proved of the strong biofilm formation. Previous literature also showed of the exhibition of biofilm formation of the Streptomyces species. The increase or stability in the values proves of the possible cooperative role of both the species in the antagonistic activity against the Nigrospora species. A one way ANOVA was done to show the significance in the formation of biofilms. Peculiar results were obtained in the TLC. The Rf values obtained were compared with the standard antioxidant Rf values. The Rf values of 0.12 match with the catechin, and 0.4 match with the Quercetin, 06 match with Caffeic acid.
Antagonistic Activity and Biofilm Studies on Streptomyces Species towards Nigrospora Sp, American Journal of Life Sciences. Special Issue: Recent Developments in Health Care through Plants and Microbes.
Vol. 2, No. 6-1,
2014, pp. 28-34.
M Zarei, S Aminzadeh, H Zolgharnein, A Safahieh, M Daliri, K A Noghabi, A Ghoroghi, A Motallebi, 2011 , Characterization Of A Chitinase With Antifungal Activity From A Native Serratia Marcescens B4a, Brazilian Journal of Microbiology 42: 1017-1029
AL Garda, JMF Abalos, P Sanchez, A R Arribas, RI Santamaria, 1997, Two genes encoding an endoglucanase and a cellulose-binding protein are clustered and co-regulated by a TTA codon in Streptomyces halstedii JM8. Biochem J 324:403–411.
G Duan, N Christian, J Schwachtje, D Walther, and O Ebenhöh, 2013. The Metabolic Interplay between Plants and Phytopathogens, Metabolites, 3, 1-23.
G Vaaje-Kolstad, S J Horn, M. F Daan, B Synstad, and Vincent G. H. Eijsink 2005, The Non-catalytic Chitin-binding Protein CBP21 from Serratia marcescens Is Essential for Chitin Degradation J. Biol. Chem. 2005, 280:28492-28497.
B Nazari, M Kobayashi, A Saito, A Hassaninasa, K Miyashita, T Fujiia 2013, Chitin-Induced Gene Expression in Secondary Metabolic Pathways of Streptomyces coelicolor A3(2) Grown in Soil. Applied and Environmental Microbiology p. Volume 79, 707–713.
B Prapagdee, C Kuekulvong and S Mongkolsuk, 2008, Antifungal Potential of Extracellular Metabolites Produced by Streptomyces hygroscopicus against Phytopathogenic Fungi, Int. J. Biol. Sci., 4. 330-337.
B. B. Aam, E. B. Heggset , A. L Norberg, M. S. Kjell, M. Varum and V. G. H. Eijsink, 2010, Production of Chitooligosaccharides and their potential Applications in Medicine, Mar. Drugs, 8, 1482-1517
C Shi, P Yan, J Li, H Wu, Q Li and S Guan 2014. Biocontrol of Fusarium graminearum Growth and Deoxynivalenol Production in Wheat Kernels with Bacterial Antagonists, Int. J. Environ. Res. Public Health, 11, 1094-1105.
M. A Hannan, M. M. Hasan, I. Hossain1, S. M. E. Rahman, A M Ismail, and Deog-Hwan Oh, 2012, Integrated Management of Foot Rot of Lentil Using Biocontrol Agents under Field Condition. J. Microbiol. Biotechnol., 22(7), 883–888.
A. T. Arana, J. Valle, C. Solano, M.A Jesu´ S Arrizubieta, C Cucarella, M Lamata, B. Amorena, J. Leiva, Jose´ R. Penade, And In Igo Lasa, 2001, The Enterococcal Surface Protein, Esp, Is Involved in Enterococcus faecalis Biofilm Formation, Appl. Environ. Microbiol., 67(10):4538.
K.C Hoang, T. H Lai, C. S Lin, Y. T Chen and C. Y Liau, 2011. The Chitinolytic Activities of Streptomyces sp. TH-11, Int. J. Mol. Sci., 12, 56-65.
A. S. Magdalena E. Tenconi,b S. Rigali, and P. Gilles. VWezela 2011. Functional Analysis of the N-Acetylglucosamine Metabolic Genes of Streptomyces coelicolor and Role in Control of Development and Antibiotic Production. Journal of Bacteriology p. 1136–1144.
M. S Brzezinsk U. Jankiewicz., 2012, Production of Antifungal Chitinase by Aspergillus niger LOCK 62 and Its Potential Role in the Biological Control, Curr Microbiol 65:666–672.
R. Nandakumar, S. Babu1, T. Raguchander and R. Samiyappan, 2007, Chitinolytic Activity of Native Pseudomonas fluorescens Strains J. Agric. Sci. Technol. Vol. 9: 61-68.
T. Kawase, S. Yokokawa, A. Saito; T. Fujii, N. nikaidou, K. Miyashita, and Takeshi Watanabe, 2005. Comparison of Enzymatic and Antifungal Properties between Family 18 and 19 Chitinases from S. coelicolor A3(2), Biosci. Biotechnol. Biochem., 70 (4), 988–998, 2006.
P. N. Lipke And R. Ovalle, 1998, MINIREVIEW Cell Wall Architecture in Yeast: New Structure and New Challenges J. Bacteriol, 180(15):3735.
R. Hamid, M. A. Khan, M. Ahmad, M. M. Ahmad, M. Z Abdin, J. Musarrat, and S. Javed., 2013. Chitinases: An update J Pharm Bioallied Sci. Jan-Mar; 5(1): 21–29.
S. H Ji, N. C Paul, J. X Deng, Y. S Kim, B. S Yun and S. H Yu, 2013. Biocontrol Activity of Bacillus amyloliquefaciens CNU114001 against Fungal Plant Diseases, Mycobiology December, 41(4): 234-242.
S. Subramaniam, V. Ravi, G. K Narayanan. 2012. Studies on Production of Enzyme Chitinase from Streptomyces sp. and its anti-fungal activity. Journal of Pharmacy Research,5(3),1409-1413.
T. Watanabe, W. Oyanagi, K. Suzuki, and H. Tanaka, 1990. Chitinase System of Bacillus circulans WL-12 and Importance of Chitinase Al in Chitin Degradation, J. Bacteriol. vol. 172 no. 74017-4022.
S. Rigali, F. Titgemeyer, S. Barends, S. Mulder, A. W. Thomae, D. A Hopwood & G. P. van Wezel 2008. Feast or famine: the global regulator DasR links nutrient stress to antibiotic production by Streptomyces, EMBO reports, 9, 670–675.
D.L Crawford, J.M Lynch, J.M Whipps, M.A Ousley (1993). Isolation and characterization of Actinomycete antagonists of a fungal root pathogen. Appl. Environ. Microbiol. 59: 3899-3905.
Sutthinan Khamna, Akira Yokota, John F Peberdy, Saisamorn Lumyong 1, 2009. Antifungal activity of Streptomyces spp. isolated from rhizosphere of Thai medicinal plants, International Journal of Integrative Biology. Vol. 6, No. 3, 143.
K. Kadokura , A. Rokutani, M. Yamamoto, T. Ikegami, H. Sugita, S. Itoi, W. Hakamata, T. Oku, T. Nishio. 2007. Purification and characterization of Vibrio parahaemolyticus extracellular chitinase and chitin oligosaccharide deacetylase involved in the production of heterodisaccharide from chitin. Appl Microbiol Biotechnol. 75: 357–365.
J. H. Benson. Microbiological Applications : Laboratory Manual in General Microbiology, Short Version, 8th Edition; Published by McGraw Hill.
O. H Lowry, N.J Rosebrough, A.L Farr, R. J Randall (November 1951). "Protein measurement with the Folin phenol reagent". J. Biol. Chem. 193 (1): 265–75.