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Potential of Silver Nano Particles Synthesized from Ficus sycomorus Linn Against Multidrug Resistant Shigella species Isolated from Clinical Specimens
American Journal of Life Sciences
Volume 8, Issue 4, August 2020, Pages: 82-90
Received: Mar. 3, 2020; Accepted: Mar. 24, 2020; Published: Aug. 25, 2020
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Authors
Yunusa Saheed, Desert Research Monitoring and Control Centre, Yobe State University, Damaturu, Nigeria
Ahmed Faruk Umar, Microbiology Department, Abubakar Tafawa Balewa University, Bauchi, Nigeria
Mahmud Yerima Iliyasu, Microbiology Department, Abubakar Tafawa Balewa University, Bauchi, Nigeria
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Abstract
Ficus sycomorus Plant was known traditionally for its medicinal properties, Shigella species as a bacterial was also known for their resistance to orthodox medicine. Hence the synthesis of silver nanoparticles from Ficus sycomorus. This study was carried out to investigate the anti-shigellosis potential of silver nanoparticles synthesized from Ficus sycomorus Linn stem bark aqueous extract against Multi-drug Resistant (MDR) Shigella species isolated from clinical specimen collected from patients attending Yobe State Specialist Hospital Damaturu, Nigeria. A total of 400 diarrhoeagenic stools were screened for isolation of Shigella species and determined their antibiotic susceptibility pattern using standard methods. Phytochemical constituents of Ficus sycomorus extract were used to synthesize silver nanoparticles using green synthesis approach. The nanoparticles was analyzed for transmittance, functional groups, sizes and shapes using Uv-vis, FTIR and Scanning Electron Microscopy (SEM), and was tested for antibacterial activities on MDR Shigella isolates. There is no significant difference in Shigella recovery relation to patients gender (P<0.05). The age group, 0 - 10 years were more susceptible, 40% (36), followed by >30 years (21). Shigella were also found to be sensitive to Ciprofloxacin (92%), Augmentin (87%), Cefuroxime (85%), Streptomycin (83.5%) while the most frequent resistance was showcased against Nalidixic Acid (48%), and Tetracycline (27%). Phytochemicals detected include saponins, flavonoids, alkaloids, cardiac glycoside and tannin. Uv-vis showed broad peaks around 460nm, the FTIR showed C-H stretch of hydroxyl group of alkanes and the SEM showed nanoparticles with wide range of shapes and sizes. Anti-Shigella activities of silver nanoparticles is higher at zones of inhibition between 10mm and 30mm higher compared to the activities of crude aqueous extract and AgNO3 solution against the MDR Shigella species which showed an enhanced activities. The high prevalence of shigellosis among children in this study, indicated that improved hygiene is needed for children in the area and detailed examination is required for the treatment of diarrhoea in adults. Ciprofloxacin and Amoxicillin Clavulanate, Nalidixic acid could be used only where culture and sensitivity results prevailed. Enhanced traditional medicine should be given priority because of its potentials. This study have demonstrated feasibility of the green synthesis of F. sycomorus as a potent anti-shigellosis to combat the global burden of the disease. This is the first study On Stem bark aqueous extracts of F. sycomorus against Shigella species in the area.
Keywords
Nanoparticles, Shigellosis, Diarrhoeagenic, Damaturu, Ficus sycomorus, MDR
To cite this article
Yunusa Saheed, Ahmed Faruk Umar, Mahmud Yerima Iliyasu, Potential of Silver Nano Particles Synthesized from Ficus sycomorus Linn Against Multidrug Resistant Shigella species Isolated from Clinical Specimens, American Journal of Life Sciences. Vol. 8, No. 4, 2020, pp. 82-90. doi: 10.11648/j.ajls.20200804.16
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Copyright © 2020 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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