A Quantitative Study of Nocodazole’S Effect on HeLa Cells’ Growth Rate and F-actin Structure

Jiayi Lu; Jorge De La Torre; Carson McCann; Maureen Madar; Qiongqiong Zhou

doi:doi:10.11648/j.ajls.20180601.12

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A Quantitative Study of Nocodazole’S Effect on HeLa Cells’ Growth Rate and F-actin Structure

Jiayi Lu, Jorge De La Torre, Carson McCann, Maureen Madar, Qiongqiong Zhou

Received: 7 March 2018 Accepted: Published: 9 March 2018

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Abstract

HeLa cells were derived from human cervical cancer, which has been widely used as research model to study mammalian cell functions. In this work, nocodazole, a microtubule destabilizer, was used to treat HeLa cells with different concentrations. The results showed that nocodazole was able to inhibit HeLa cell’s growth rate significantly at the concentration of 100 nM. It suggested that nocodazole may inhibit cell growth through an alternative impacting effect other than destabilizing microtubules, since the effect of nocodazole destabilizing microtubule is usually not seen at micromolar range. However, at nanomolar concentration, nocodazole was not able to induce any changes in F-actin structure. Whereas, at concentration of 1 mM, nocodazole induced significant alterations of F-actin structure in HeLa cells. It indicated the strong relationship between microtubule and actin dynamics. Our work suggested that a molecule may exhibit different impacting mechanisms at different concentrations. Since nocodazole has been used as a chemotherapy reagent in cancer treatment, it will be beneficial to re-evaluate the effective concentration in terms of cancer treatment.

DOI	10.11648/j.ajls.20180601.12
Published in	American Journal of Life Sciences (Volume 6, Issue 1, February 2018)
Page(s)	7-12
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Nocodazole, F-actin, Microtubules, Growth Rate, HeLa Cells

References

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APA Style

Jiayi Lu, Jorge De La Torre, Carson McCann, Maureen Madar, Qiongqiong Zhou. (2018). A Quantitative Study of Nocodazole’S Effect on HeLa Cells’ Growth Rate and F-actin Structure. American Journal of Life Sciences, 6(1), 7-12. https://doi.org/10.11648/j.ajls.20180601.12

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ACS Style

Jiayi Lu; Jorge De La Torre; Carson McCann; Maureen Madar; Qiongqiong Zhou. A Quantitative Study of Nocodazole’S Effect on HeLa Cells’ Growth Rate and F-actin Structure. Am. J. Life Sci. 2018, 6(1), 7-12. doi: 10.11648/j.ajls.20180601.12

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AMA Style

Jiayi Lu, Jorge De La Torre, Carson McCann, Maureen Madar, Qiongqiong Zhou. A Quantitative Study of Nocodazole’S Effect on HeLa Cells’ Growth Rate and F-actin Structure. Am J Life Sci. 2018;6(1):7-12. doi: 10.11648/j.ajls.20180601.12

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@article{10.11648/j.ajls.20180601.12,
  author = {Jiayi Lu and Jorge De La Torre and Carson McCann and Maureen Madar and Qiongqiong Zhou},
  title = {A Quantitative Study of Nocodazole’S Effect on HeLa Cells’ Growth Rate and F-actin Structure},
  journal = {American Journal of Life Sciences},
  volume = {6},
  number = {1},
  pages = {7-12},
  doi = {10.11648/j.ajls.20180601.12},
  url = {https://doi.org/10.11648/j.ajls.20180601.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajls.20180601.12},
  abstract = {HeLa cells were derived from human cervical cancer, which has been widely used as research model to study mammalian cell functions. In this work, nocodazole, a microtubule destabilizer, was used to treat HeLa cells with different concentrations. The results showed that nocodazole was able to inhibit HeLa cell’s growth rate significantly at the concentration of 100 nM. It suggested that nocodazole may inhibit cell growth through an alternative impacting effect other than destabilizing microtubules, since the effect of nocodazole destabilizing microtubule is usually not seen at micromolar range. However, at nanomolar concentration, nocodazole was not able to induce any changes in F-actin structure. Whereas, at concentration of 1 mM, nocodazole induced significant alterations of F-actin structure in HeLa cells. It indicated the strong relationship between microtubule and actin dynamics. Our work suggested that a molecule may exhibit different impacting mechanisms at different concentrations. Since nocodazole has been used as a chemotherapy reagent in cancer treatment, it will be beneficial to re-evaluate the effective concentration in terms of cancer treatment.},
 year = {2018}
}

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TY - JOUR
T1 - A Quantitative Study of Nocodazole’S Effect on HeLa Cells’ Growth Rate and F-actin Structure
AU - Jiayi Lu
AU - Jorge De La Torre
AU - Carson McCann
AU - Maureen Madar
AU - Qiongqiong Zhou
Y1 - 2018/03/09
PY - 2018
N1 - https://doi.org/10.11648/j.ajls.20180601.12
DO - 10.11648/j.ajls.20180601.12
T2 - American Journal of Life Sciences
JF - American Journal of Life Sciences
JO - American Journal of Life Sciences
SP - 7
EP - 12
PB - Science Publishing Group
SN - 2328-5737
UR - https://doi.org/10.11648/j.ajls.20180601.12
AB - HeLa cells were derived from human cervical cancer, which has been widely used as research model to study mammalian cell functions. In this work, nocodazole, a microtubule destabilizer, was used to treat HeLa cells with different concentrations. The results showed that nocodazole was able to inhibit HeLa cell’s growth rate significantly at the concentration of 100 nM. It suggested that nocodazole may inhibit cell growth through an alternative impacting effect other than destabilizing microtubules, since the effect of nocodazole destabilizing microtubule is usually not seen at micromolar range. However, at nanomolar concentration, nocodazole was not able to induce any changes in F-actin structure. Whereas, at concentration of 1 mM, nocodazole induced significant alterations of F-actin structure in HeLa cells. It indicated the strong relationship between microtubule and actin dynamics. Our work suggested that a molecule may exhibit different impacting mechanisms at different concentrations. Since nocodazole has been used as a chemotherapy reagent in cancer treatment, it will be beneficial to re-evaluate the effective concentration in terms of cancer treatment.
VL - 6
IS - 1
ER -

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Author Information

Jiayi Lu

Department of Biological Sciences, Denison University, Granville, USA
Jorge De La Torre

Department of Biological Sciences, Denison University, Granville, USA
Carson McCann

Department of Biological Sciences, Denison University, Granville, USA
Maureen Madar

Department of Biological Sciences, Denison University, Granville, USA
Qiongqiong Zhou

Department of Biological Sciences, Denison University, Granville, USA

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Plain Text BibTeX RIS

APA Style

Jiayi Lu, Jorge De La Torre, Carson McCann, Maureen Madar, Qiongqiong Zhou. (2018). A Quantitative Study of Nocodazole’S Effect on HeLa Cells’ Growth Rate and F-actin Structure. American Journal of Life Sciences, 6(1), 7-12. https://doi.org/10.11648/j.ajls.20180601.12

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ACS Style

Jiayi Lu; Jorge De La Torre; Carson McCann; Maureen Madar; Qiongqiong Zhou. A Quantitative Study of Nocodazole’S Effect on HeLa Cells’ Growth Rate and F-actin Structure. Am. J. Life Sci. 2018, 6(1), 7-12. doi: 10.11648/j.ajls.20180601.12

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AMA Style

Jiayi Lu, Jorge De La Torre, Carson McCann, Maureen Madar, Qiongqiong Zhou. A Quantitative Study of Nocodazole’S Effect on HeLa Cells’ Growth Rate and F-actin Structure. Am J Life Sci. 2018;6(1):7-12. doi: 10.11648/j.ajls.20180601.12

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@article{10.11648/j.ajls.20180601.12,
  author = {Jiayi Lu and Jorge De La Torre and Carson McCann and Maureen Madar and Qiongqiong Zhou},
  title = {A Quantitative Study of Nocodazole’S Effect on HeLa Cells’ Growth Rate and F-actin Structure},
  journal = {American Journal of Life Sciences},
  volume = {6},
  number = {1},
  pages = {7-12},
  doi = {10.11648/j.ajls.20180601.12},
  url = {https://doi.org/10.11648/j.ajls.20180601.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajls.20180601.12},
  abstract = {HeLa cells were derived from human cervical cancer, which has been widely used as research model to study mammalian cell functions. In this work, nocodazole, a microtubule destabilizer, was used to treat HeLa cells with different concentrations. The results showed that nocodazole was able to inhibit HeLa cell’s growth rate significantly at the concentration of 100 nM. It suggested that nocodazole may inhibit cell growth through an alternative impacting effect other than destabilizing microtubules, since the effect of nocodazole destabilizing microtubule is usually not seen at micromolar range. However, at nanomolar concentration, nocodazole was not able to induce any changes in F-actin structure. Whereas, at concentration of 1 mM, nocodazole induced significant alterations of F-actin structure in HeLa cells. It indicated the strong relationship between microtubule and actin dynamics. Our work suggested that a molecule may exhibit different impacting mechanisms at different concentrations. Since nocodazole has been used as a chemotherapy reagent in cancer treatment, it will be beneficial to re-evaluate the effective concentration in terms of cancer treatment.},
 year = {2018}
}

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