Shigella Serogroups, Entro-Hemoragic E. coli and Their Antibiogram Pattern Among Food Handlers in Food-Handling Establishments in Southern Ethiopia
American Journal of Life Sciences
Volume 5, Issue 2, April 2017, Pages: 46-51
Received: Feb. 8, 2017; Accepted: Feb. 18, 2017; Published: Mar. 11, 2017
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Fiseha Wadillo Wada, School of Medicine, College of Health Sciences and Medicine, Wolaita Sodo University, Wolaita Sodo, Ethiopia
Tesfahun Molla Bobe, School of Medicine, College of Health Sciences and Medicine, Wolaita Sodo University, Wolaita Sodo, Ethiopia
Habtamu Azene Tekle, School of Medicine, College of Health Sciences and Medicine, Wolaita Sodo University, Wolaita Sodo, Ethiopia
Tamene Naba Yaya, School of Medicine, College of Health Sciences and Medicine, Wolaita Sodo University, Wolaita Sodo, Ethiopia
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Food-borne illnesses have a dramatic impact both in developing and developed countries. Food handling personnel take part in the transmission of pathogenic food born bacteria in the community. Shigella and E. coli 157:H7 are more significant and well-recognized foodborne pathogens for reasons of their severe consequences of all age groups, high antibiotic resistance and their low infectious dose. Accordingly, food-handlers employing in meal serving facilities could be potential sources of infections of these enteric bacterial pathogens. Community based cross-sectional study was carried out from July 2014 to June 2015 to assess the sero-group and antimicrobial resistance pattern of enteric bacterial pathogens in Wolaita Sodo town among 398 food-handlers working in selected food handling establishments. Pre-tested structured questionnaire was used to collect Socio-demographic characteristics and associated factors. Stool specimens were collected by a clean, dry, wide-mouthed container. Stool culture was done using differential, selective and enrichment medium. Analytical Profile Index 20E biochemical panel was used for identification and differentiation of members of enteric bacterial pathogens. Antibiotic susceptibility testing was done by single disk diffusion technique. Data entry and analysis were done using SPSS version 20. 11 Shigella species and 24 E. coli O157:H7 isolates were detected. S. flexneri, S. sonnei, S. dysenteriae, and S. boydii isolates were isolated. A significant proportion of Ampicillin and Amoxicillin were noticed for all enteric bacterial pathogens. Multidrug resistances prevalence of 72.7%, and 58.3% were observed for Shigella, and E. coli O157: H7 respectively. Raw meat eating habit, hand washing after toilet and hand washing after touching dirty materials have shown significant association with enteric bacterial pathogens prevalence. Shigella and E. coli species were identified from fecal specimen. Significant proportion of multidrug resistances was detected in Shigella and E. coli O157: H7 respectively. Thus screening of food handles is important in order to prevent the transmission of enteric bacterial pathogens and treatment needs to be based on accurate laboratory detection of etiologic agents to mitigate the spread of drug resistant strains.
E. coli O157:H7, Shigella, Food Handlers, Antibiotics
To cite this article
Fiseha Wadillo Wada, Tesfahun Molla Bobe, Habtamu Azene Tekle, Tamene Naba Yaya, Shigella Serogroups, Entro-Hemoragic E. coli and Their Antibiogram Pattern Among Food Handlers in Food-Handling Establishments in Southern Ethiopia, American Journal of Life Sciences. Vol. 5, No. 2, 2017, pp. 46-51. doi: 10.11648/j.ajls.20170502.12
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Nigusse, D and Kumie, A. (2012): Food hygiene practices and prevalence of intestinal parasites among food handlers working in Mekelle University student’s cafeteria, Mekelle. Global Advanced Research Journal of Social Science (GARJSS), 1 (4): 65-71.
Tefera, T, and Mebrie, G. (2014): Prevalence and Predictors of Intestinal Parasites among Food Handlers in Yebu Town, Southwest Ethiopia. PLoS ONE. 9 (10):.
Centers for disease control and prevention. Food borne Germs and Illnesses. Available at Accessed march 13, 2015.
Neal G. M. (1996): Escherichia, Klebsiella, Enterobacter, Serratia, Citrobacter, and Proteus. Medical Microbiology. 4th edition.
Mokhtari, W., Nsaibia, S., Majouri, D., Hassen, A. B., Gharbi A, and Aouni M.(2012): Detection and characterization of Shigella species isolated from food and human stool samples in Nabeul, Tunisia, by molecular methods and culture techniques. Journal of Applied Microbiology. 113: 209–222.
Trevejo T., Abbott L., Wolfe I.,Meshulam J., Yong D., and Flores G. R. (1999): An UntypeableShigellaflexneri strain associated with an outbreak in California. Journal of Clinical Microbiology. 37 (7): 2352-53.
Centers for Disease Control and Prevention (2003): Preliminary Food Net data on the incidence of food-borne illnesses-selected sites, United States, 2002. Morb Mortal Wkly Rep.52: 340–3.
Dagnew, M, Tiruneh, M, and Moges, F (2013): Bacterial Profile and Antimicrobial Susceptibility Pattern among Food Handlers at Gondar University Cafeteria, Northwest Ethiopia. J Infect Dis Ther.1: 105.
Andargie.,G, Kassu., A, Moges., F, and Huruy, K.(2008): Prevalence of bacteria and intestinal parasites among food-handlers in Gondar town, northwest Ethiopia. J Health PopulNutr. 26: 451–5.
Omemu, A. and Oloyede, F. (2014): Assessment of the hygienic practices and the incidence of enteric bacteria in food handlers in small businesses in an urban area in Abeokuta. 5 (3): 41-49.
Maritha, M., Nelene, C., and Demetre, L(2007): Small and micro enterprises – aspects of knowledge, attitudes and practices of managers’ and food handlers’ knowledge of food safety in the proximity of Tygerberg Academic Hospital, Western Cape. SAJCN. 20 (2): 50-61.
Identification system for Enterobacteriaceae and other non-fastidious Gram-negative rodsREF 20 100 / 20 160.api®20 E 07584D- GB - 2002/10.
CLSI 2014 Performance Standards for Antimicrobial Susceptibility Testing; Twenty-Second Informational Supplements. CLSI Document M100-S22. Wayne. Pa: Clinical and Laboratory Standards Institute.
Aklilu, A., Kahase, D., and Dessalegn, M. (2015): Prevalence of intestinal parasites, salmonella and shigella among apparently health food handlers of Addis Ababa University student’s cafeteria, Addis Ababa, Ethiopia. BMC Research Notes. 8: 17.
Muna, A, Renad, A,andRehan B.(2014): Microbiological and parasitological investigation among food handlers in hotels in the Dead Sea area, Jordan. Journal of Microbiology, Immunology and Infection. 47: 377-80.
Andualem, T. D., Tiruneh, M. W., Moges, F. Y., Muluye, D. F., Ayalew G. G (2014): Prevalence and antimicrobial susceptibility patterns of Shigella and Salmonella Species among patients with diarrhea attending Gondar town health institutions, Northwest Ethiopia. Science Journal of Public Health. 2 (5): 469-75.
Tiruneh, M. (2009): Sero-diversity and antimicrobial resistance pattern of Shigella isolates at Gondar University Teaching Hospital, Northwest Ethiopia. JpnJ Infect Dis.62: 93-7.
Asrat, D. (2008): Shigella and Salmonella serogroups and their antibiotic susceptibility patterns in Ethiopia. East Mediterr Health J. 14: 760-7.
Opintan, J, and Newman, M. J. (2007): Distribution of serogroups and serotypes of multiple drug resistant Shigella isolates. Ghana Med J. 41: 8-29.
Mengistu, G., Mulugeta, G., LemaT.,andAssefa A (2014): Prevalence and Antimicrobial Susceptibility Patterns of Salmonella serovars and Shigella species. J MicrobBiochemTechnol.
Gebrekidan, A, Asmelash T, and Kahsay G. (2015). GebreysusA. W. Prevalence and antimicrobial susceptibility patterns of Shigella among acute diarrheal outpatients in Mekelle hospital, Northern Ethiopia. BMC Res Notes. 8: 611.
Mamuye,Y., Metaferia, G., Birhanu, A., Desta, K., and Fantaw, S.(2015) Isolation and Antibiotic Susceptibility Patterns of Shigella and Salmonella among Under 5 Children with Acute Diarrhoea: A Cross-Sectional Study at Selected Public Health Facilities in Addis Ababa, Ethiopia. ClinMicrobiol. 4: 186.
Adugna, A., Kibret, M., Nibret, E., and Abera, B. (2015). Antibiogram of E. coli serotypes isolated from children aged under five with acute diarrhea in Bahir Dar town. African Health Sciences. 15 (2): 656-64.
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