Characterization of Myostatin Gene in Nile Tilapia (Oreochromis niloticus), the Possible Association of BsmI-exon 2 Polymorphism with Its Growth
American Journal of Life Sciences
Volume 4, Issue 3, June 2016, Pages: 82-86
Received: Apr. 30, 2016; Accepted: Jun. 1, 2016; Published: Jun. 17, 2016
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Nasema A. Elkatatny, Biotechnology Department, Animal Health Research Institute-Kafer El-Shik, Egypt
Zizy I. Elbialy, Biotechnology Department, Animal Health Research Institute-Kafer El-Shik, Egypt
Abeer F. El-Nahas, Animal Wealth and Animal Husbandry Department, Faculty of Veterinary Medicine, Alexandria University, Egypt
Shawky Mahmoud, Department of Physiology, Faculty of Veterinary Medicine, Kafer El Sheikh University, Egypt
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Myostatin (MSTN) gene is a negative regulator of skeletal muscle. In this study, we try to evaluate MSTN as a candidate gene for a marker assisted selection in Nile tilapia growth trait. Molecular characterization of MSTN gene was done using seven designed primers. We demonstrated that, O. niloticus MSTN sequence and its promoter is as for all known vertebrates. Novel SNPs were identified in coding and non-coding regions compared with MSTN gene of O. niloticus x O. aureus hybrid, three non-synonymous SNPs were found at MSTN coding region; two at exon one 369 C>A, 831 T>A, and one at exon 3 2637 G>A, altering Thr 38-Pro, Glu 121-Val and Tyr 375-Cys respectively. Phylogenetic analysis revealed high similarity (99.2) with MSTN gene of the hybrid with O. aurous. BsmI induced cutting pattern at MSTN-exon 2 (607-bp). Among two hundred monosex male fish, two different genotypes were reported; AB genotype (607-bp, 507-bp and 100-bp) and BB genotype (507-bp and 100-bp fragments), were produced. Most large-size fish are included in AB genotype with 0.8 frequency and significantly increased body weight compared with small size fish, which are mostly included in BB genotype with 0.9 frequencies. Novel BsmI-exon 2 polymorphism of MSTN gene can be used as a marker assisted selection for large body weight in heterozygous Nile tilapia fish.
Nile Tilapia, Myostatin Gene, Polymorphism, SNPs, RFLP
To cite this article
Nasema A. Elkatatny, Zizy I. Elbialy, Abeer F. El-Nahas, Shawky Mahmoud, Characterization of Myostatin Gene in Nile Tilapia (Oreochromis niloticus), the Possible Association of BsmI-exon 2 Polymorphism with Its Growth, American Journal of Life Sciences. Vol. 4, No. 3, 2016, pp. 82-86. doi: 10.11648/j.ajls.20160403.13
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