Profile of Dermatophyte and Non Dermatophyte Fungi in Patients Suspected of Dermatophytosis
American Journal of Life Sciences
Volume 3, Issue 5, October 2015, Pages: 352-357
Received: Aug. 1, 2015;
Accepted: Aug. 12, 2015;
Published: Aug. 21, 2015
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Gebreabiezgi Teklebirhan, Tikur Anbessa Hospital, College of Health Sciences, Addis Ababa University, Addis Ababa, Ethiopia
Adane Bitew, Department of Medical Laboratory Sciences, College of Health Sciences, Addis Ababa University, Addis Ababa, Ethiopia
Fungal infections of hair, nail and skin are common worldwide and continue to increase. The present study was undertaken to determine the prevalence of dermatophytosis, isolation rates and the profile of fungi associated with dermatophytosis. Samples were collected from 305 patients and a portion of each sample was examined microscopically and the remaining portion was cultured on to plates of Sabouraud’s Dextrose Agar containing chloramphinical with and without cychloheximide. Fungal cultures were identified by studying macroscopic and microscopic characteristics of their colonies. Of 305 clinical samples, fungi were detected in 265 (86.9%) samples by KOH and 224 (73.4%) clinical samples were culture positive. Dermatophytes were the most common isolates accounting 130 (58.0%) of the total isolates and this was followed by non- dermatophyte molds and yeasts that accounted 49 (21.9 %.) and 45 (20.0%) respectively. T. violaceum was the dominant species accounting for 49 (37.7%) dermatophyte isolates. Candida albicans was the dominant species accounting 30 (66.7%) of the total yeast isolates. The genus Aspergillus was the most common non-dermatophyte molds consisting of 13 (26.5%) species. Tinea unguium was the predominant clinical manifestation accounting 51.1% of the cases of which 119 (76.3%) were from females and 37 (23.7%) from males. T. violaceum was the most common pathogen in tinea unguium and tinea capitis, whereas T. mentagrophytes was the most common pathogen in tinea pedis and tinea manum. Seventy five percent of yeasts and 77.6% non dermatophyte molds were isolated from nails. Along with dermatophytes, non-dermatophyte fungi are also emerging as important causes of dermatophytosis. Both direct microscopy and culture are important tools for diagnosis of the fungal infections.
Profile of Dermatophyte and Non Dermatophyte Fungi in Patients Suspected of Dermatophytosis, American Journal of Life Sciences.
Vol. 3, No. 5,
2015, pp. 352-357.
Havlickova B, Czaika VA, Friedrich M. Epidemiological trends in skin mycoses worldwide. Mycoses 2008; 51:2-15.
Popoola SOT, OJO AD, Alabi OR. Prevalence of dermatophytosis in junior secondary school children in Ogun State, Nigeria. Mycoses 2006; 49: 499-503.
Ameen M. Epidemiology of superficial fungal infections. Clin. Dermatol. 2010; 28:197-201.
Nweze EI. Dermatophytosis among children of Fulani/Hausa living in South Eastern Nigeria. RevistaIberoamericana de Micol. 2010: 27: 191-194.
Adefemi SA, Odeigah LO, Alabi KM. Prevalence of dermatophytosis among primary school children in Oke-oyi community of Kwara state. Nigerian J. Clin. Practice 2011; 14: 23-28.
Al Shekh H. Epidemiology of dermatophtes in the eastern province of Saudi Arabia. Res. J. Micobiol 2009; 4: 229-234.
Erbagci Z, Tuncel AA, Yasemin ZY, Balci I. A prospective epidemiologic survey on the prevalence of onychomycosis and dermatophytosis in male boarding school residents Mycopathologia 2005; 159: 347–35.
Beena S, Sreeja. MV, Bhavana PR, SreenivasaBabu S. Onychomycosis: prevalence and its etiology in a tertiary care hospital, south India. Int. J. Health Sci. Res 2013; 3::81-85.
Satpathi P, Achar A, Banerjee D, Maiti A,Sengupta M, Mohata A. Onychomycosis in EasternIndia - study in a peripheral tertiary care centre. J. Pakistan Assoc. Dermatol 2013; 23:14-19.
Asadi MA, Dehghan R, Sharif. MR.Epidemiologic study of onychomycosis and tinea pedis in Kashan, Iran. Jundishapur J. Microbiol 2009; 2: 61-64.
Bramono K, Budjmlja U. Epidiomology of onychomycosis in Indonesia: Data obtained from three Indonian studies. Jpn. J. Mycol 2005; 46: 171-176.
Hay RJ. Fungal infections. In: Warrell DA, Cox TM, Firth JD and Benz EJ Jr. (eds). Oxford Textbook of Medicine, ed 4. Oxford University Press, Oxford, 2003; P. 7.12.1.
Ilkit M. Favus of the scalp: an overview and update. Mycopathologia 2010; 170: 143-154.
Figueroa JI, Hawranek T, Abraha A, Hay RJ. Tinea capitis in Southwestern Ethiopia: a study of risk factors for infection and carriage. Int. J. Dermatol 1997; 36: 661–6.
Figueroa JL, Hawranek T, Abraha A, Hay RJ. Dermatology in Southwestern Ethiopia: rationale for a community approach. Int. J. Dermatol 1998; 37: 752–8.
Woldeamanuel Y. Mengistu Y, Chryssanthou E, Petrini B. Dermatophytosis in Tulugudu Island, Ethiopia, Med. Mycol. 2005; 43; 79-82.
Woldeamanuel Y, Leekassa R, Chryssanthou E, Mengistu Y, Petrini B. Prevalence of Tinea capitis in Ethiopoan school children. Mycoses 2005; 45: 137-41.
Kern M. Medical mycology, a self instructional text. Philadelphia, F. D Davis Company, 2nd ed. 1985.
Mohanty JC, Mohanty SK, Sahoo RC et al. Diagnosis of superficial mycoses by direct microscopy – a statistical evaluation. IJDV, 1999; 65: 72 – 4.
Kannan P, Janaki C, Selvi GS. Prevalence of dermatophytes and other fungal agents isolated from clinical specimens. Indian J Med. Microbiol 2006; 24 (3):212-215.
Ndako JA, Osemwegie OO, Spencer THI, Olopade BK, Yunusa GA, Banda J. Prevalence of Dermatophytes and other associated Fungi among school children. Global Advanced Res. J. Medicine and Medical Sci 2012; 1(3): 049-056.
Gangoom AM, Elyazachi MB, Al-Ani SM, Duwb GA. Tinea capitis in Benghazi, Libya. Int. J.Dermatol 2000; 39: 263–5.
Ellabib MS, Agaj M, Khalifa Z, Kavanagh K. Trichophyton violaceum is the dominant cause of tinea capitis in children in Tripoli, Libya: results from a two-year survey. Mycopathologia 2002; 153: 145–7.
Ali-Shtayeh MS, Salameh AA, Abu-Ghdeib SI, Jamous RM, Khrahir H. Prevalence of tinea capitis as well as asymptomatic carriers in school children in Nablus area (Palestine). Mycoses 2002; 45: 188–94.
Hussain I, Aman S, Haroon TS, Jahangir M, Nagi AH. Tinea capitis in Lahore, Pakistan. Int.J.Dermatol 1994; 33: 255–7.
Chepchirchir A, BiiC,Ndinya -Achola JO.Dermatophyte infections in primary school children in Kibera Slums of Nairobi. East African Medical J. 2009; 86: 60-68.
Shelley S. M, Liliana M, Andrew S, Bernard C, William G. M. Isolation of Trichophyton violaceum and Trichophyton soudanense in Baltimore, Maryland. J. Clin. Microbiol. 2007; 45:461.
Shahla BN, Effat K, Mehdi AA. Study Of Dermatophytosis Infections In Dermatology Clinic Of Sina Hospital – Tabriz. Ege Tıp Dergisi, 2007; 46: 21 – 25.
Meritxell PG, Josep MT, Antoni M, Sonia S, Gemma G, Laura T, Marta P. Prevalence of tinea pedis, tinea unguium of toenails and tinea capitis in school Children from Barcelona. Rev Iberoam Micol 2009; 26:228–232.
Reddy KN, Srikanth BA, Ram Sharan TR, Biradar PM. American J. Dermatol. And Venereol 2012; 1: 35-40.
Lone R., Showkat HR, Bashir D, Khursheed S, Hussain A, Sarmast RH. ClinicoMycological Pattern of Onychomycosis. Eur J. Gen. Med 2013; 10:150-153.
Balakumar S, Rajan S,Thirunalasundari T, Solomon Jeeva S. Epidemiology of dermatophytosis in and around Tiruchirapalli, Tamilnadu, India. Asian Pac. J. Trop. Dis 2012; 2: 286-289.
Rassai1 S, Feily A, Sina N, Derakhshanmehr F. Some Epidemiological Aspects of Dermatophyte Infections in Southwest Iran. Acta Dermato venerol Croat.2011; 19:13-15.
Maraki S, Nioti E, Mantadakis E, Tselentis Y.A 7-year survey of dermatophytoses in Crete, Greece. Mycoses 2007; 50: 481-484.
Devliotou-Panagiotidou D, Koussidou-Eremondi T, Badillet G. Dermatophytosis in North Greece during the decade 1981-1990. Mycoses 1995; 38: 151- 157.
Vena GA, Chieco P, Posa F,Garofalo A, Bosco A, Cassano N. Epidemiology of dermatophytoses: retrospective analysis from 2005 - 2010 and comparison with previous data from 1975. New Microbilogica. 2012; 35: 207-213.
Wahab AS, Al-Fouzan, Nanda A, kubec K. Dermatophytosis of children in Kuwait: A prospective study. Int. J. Dermatol.1993; 32: 798- 801.
Attapattu MC. A study of tinea capitis in Sri-lanka. J. Medical and Veterinary Mycol 1989; 27: 27-32.
Oliveira JAA, Barros JA, Cortez ACA, Oliveira JSRL. Superficial mycoses in the city of Manaus. An. Bras Dermatol 2006; 238-243.
David G, Richard C.B et al. Medical Microbiology: a guide to microbial infections, pathogenesis, immunity, laboratory diagnosis and control. Churchill Livingstone. Ed 16. 2003; 568-579.
Kaur R, Kashyap B, Bhalla P. Onychomycosis – Epidemiology, diagnosis and management. IJMM 2008; 26: 108 – 16.
Lange M, Nowicki R, Baranska- RybakW, Bykowska B. Dermatophytosis in children and adolescents in Ghansk, Poland. Mycoses 2003; 47: 326-329.
Caputo R, De Boulle K, Del Rosso J, Nowicki R. Prevalence of superficial fungal infections among sports-active individuals: results from the Achilles survey, a review of the literature. J. Eur Acad. Dermatol. Venereol 2001; 15:312–6.