Toxicity, Anti-Lipid Peroxidation, Invitro and Invivo Evaluation of Antioxidant Activity of Annona Muricata Ethanol Stem Bark Extract
American Journal of Life Sciences
Volume 2, Issue 5, October 2014, Pages: 271-277
Received: Oct. 6, 2014; Accepted: Oct. 15, 2014; Published: Oct. 30, 2014
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Sanni Olakunle, Department of Biochemistry, Kogi State University, Anyigba, Nigeria
Obidoa Onyechi, Department of Biochemistry, Kogi State University, Anyigba, Nigeria
Omale James, Department of Biochemistry, Kogi State University, Anyigba, Nigeria
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The presence of natural antioxidant capacity in plants has been well documented world over. There is an increasing demand for natural antioxidant to replace synthetic additives in the food and pharmacologicals. The objective of this study is to evaluate the invivo antioxidant potential of ethanol extract of Annona muricata against CCl4- induced toxicity in rats as well as its invitro antioxidant effect and lipid peroxidation. The extract was prepared by cold maceration using absolute ethanol. The invitro antioxidant properties of the extract was determined using DPPH (2,2-diphenyl-1-picrylhdrazyl) radical and invivo antioxidant enzymes were assayed to evaluate the biological activities of the extract. The polyphenol content of the extract was determined and it contained alkaloids, tannin, flavonoids, phenol in appreciable amount. In the invivo studies, the animals were grouped into three (3) groups of 15 rats each. Group 1 served as control and received 1ml/kg b.w of olive oil orally for 28 days. Group 2 rats were orally administered 1ml/kg CCl4 mixed with olive oil (1:10) daily for 28 days while group 3 rats were administered 1ml/kg CCl4 and 200 mg/kg b.w of Annona muricata stem extract. Three of the rats from each group were sacrificed on days 1, 8, 15, 22 and 28. The plant extract showed remarkable hepatoprotective and antioxidant activity against carbon tetrachloride (CCl4) induced oxidative stress as revealed from serum enzyme markers. CCl4 induced a significant rise (p<0.001) in aspartate amino transferase (AST), alanine amino transferase (ALT), alkaline phosphatase (ALP) and MDA (malondialdehyde) level in the serum with a reduction in catalase activity. Treatment of rats with the plant extract (200mg/kg b.w) significantly altered both serum enzymes activities and oxidant levels to near normal against CCl4 – treated rats. The invivo and invitro rapid radical scavenging studies were positive for the stem bark extract. This study suggests that the possible mechanism of the exhibited biological activities of the extract may be due to free radical scavenging owing to the presence of polyphenols in the extract. The plant extract possesses, antioxidant, anti-lipid peroxidation effect and is hepatoprotective. These may be the rationale for its folkloric uses and pharmacological effects.
Annona muricata, Antioxidant, DPPH (2,2-Diphenyl-1-Picrylhydrazyl), Wistar Rats, Invitro And Invivo
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Sanni Olakunle, Obidoa Onyechi, Omale James, Toxicity, Anti-Lipid Peroxidation, Invitro and Invivo Evaluation of Antioxidant Activity of Annona Muricata Ethanol Stem Bark Extract, American Journal of Life Sciences. Vol. 2, No. 5, 2014, pp. 271-277. doi: 10.11648/j.ajls.20140205.14
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