American Journal of Life Sciences

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Isolation, Characterization and Identification of Endophytic Bacteria in Maize (Zea Mays L.) Cultivated on Acrisols of the Southeast of Vietnam

Received: 11 August 2014    Accepted: 19 August 2014    Published: 30 August 2014
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Abstract

Endophytic bacterial diversity in Maize plant cultivated on Acrisols of the Eastern of South Vietnam was studied. Maize material was collected from five sites (provinces/cities) of this region. Endophytic bacteria were isolated in two kinds of medium (LGI, NFb) together with 16S rRNA gene fragments amplified from DNA using eubacterial universal primers (p515FPL and p13B). A total of 301 isolates were isolated and all of them have ability of nitrogen fixation and phosphate solubilization together with IAA biosynthesis but there were 30 isolates having the best characteristics and they were identified as maize endophytes and nifH gene owners. The sequences from selected endophytic bacteria (30 isolates) showed high degrees of similarity to those of the GenBank references strains (between 97% and 100%). Among the selected isolates were 6 isolates belong to Bacillus (20.00%) and 24 isolates belong to Proteobacteria (80.00%) including 2 alpha-proteobacteria (7%), 6 beta-proteobacteria (20%), and 16 gamma-proteobacteria (53%). Based on Pi value (nucleotide diversity), Proteobacteria group had the highest Theta values and Theta values (per sequence) from S of SNP for DNA polymorphism were calculated for each group and Proteobacteria group had the highest values in comparison of two groups. From these results showed that 3 isolates including DTN1b (Azotobacter vinelandii), VTN2b (Bacillus subtilis) and VTN7 (Enterobacter cloacae) proposed as potential microbial inoculants or biofertilizers for sustainable corn production in poor Acrisols in Vietnam because of their benefit and biosafety.

DOI 10.11648/j.ajls.20140204.16
Published in American Journal of Life Sciences (Volume 2, Issue 4, August 2014)
Page(s) 224-233
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Acrisols, 16S rRNA Gene Sequence, Endophytic Bacteria, Maize, the Eastern of South Vietnam

References
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Author Information
  • Dept. Natural Science Teacher Training, Sai Gon University, HCM city, Vietnam

  • Dept. Microbiology Biotechnology, Biotechnology R&D Institute, Can Tho University, Can Tho City, Vietnam

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    Dang Thi Ngoc Thanh, Cao Ngoc Diep. (2014). Isolation, Characterization and Identification of Endophytic Bacteria in Maize (Zea Mays L.) Cultivated on Acrisols of the Southeast of Vietnam. American Journal of Life Sciences, 2(4), 224-233. https://doi.org/10.11648/j.ajls.20140204.16

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    Dang Thi Ngoc Thanh; Cao Ngoc Diep. Isolation, Characterization and Identification of Endophytic Bacteria in Maize (Zea Mays L.) Cultivated on Acrisols of the Southeast of Vietnam. Am. J. Life Sci. 2014, 2(4), 224-233. doi: 10.11648/j.ajls.20140204.16

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    Dang Thi Ngoc Thanh, Cao Ngoc Diep. Isolation, Characterization and Identification of Endophytic Bacteria in Maize (Zea Mays L.) Cultivated on Acrisols of the Southeast of Vietnam. Am J Life Sci. 2014;2(4):224-233. doi: 10.11648/j.ajls.20140204.16

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  • @article{10.11648/j.ajls.20140204.16,
      author = {Dang Thi Ngoc Thanh and Cao Ngoc Diep},
      title = {Isolation, Characterization and Identification of Endophytic Bacteria in Maize (Zea Mays L.) Cultivated on Acrisols of the Southeast of Vietnam},
      journal = {American Journal of Life Sciences},
      volume = {2},
      number = {4},
      pages = {224-233},
      doi = {10.11648/j.ajls.20140204.16},
      url = {https://doi.org/10.11648/j.ajls.20140204.16},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.ajls.20140204.16},
      abstract = {Endophytic bacterial diversity in Maize plant cultivated on Acrisols of the Eastern of South Vietnam was studied. Maize material was collected from five sites (provinces/cities) of this region. Endophytic bacteria were isolated in two kinds of medium (LGI, NFb) together with 16S rRNA gene fragments amplified from DNA using eubacterial universal primers (p515FPL and p13B). A total of 301 isolates were isolated and all of them have ability of nitrogen fixation and phosphate solubilization together with IAA biosynthesis but there were 30 isolates having the best characteristics and they were identified as maize endophytes and nifH gene owners. The sequences from selected endophytic bacteria (30 isolates) showed high degrees of similarity to those of the GenBank references strains (between 97% and 100%). Among the selected isolates were 6 isolates belong to Bacillus (20.00%) and 24 isolates belong to Proteobacteria (80.00%) including 2 alpha-proteobacteria (7%), 6 beta-proteobacteria (20%), and 16 gamma-proteobacteria (53%). Based on Pi value (nucleotide diversity), Proteobacteria group had the highest Theta values and Theta values (per sequence) from S of SNP for DNA polymorphism were calculated for each group and Proteobacteria group had the highest values in comparison of two groups. From these results showed that 3 isolates including DTN1b (Azotobacter vinelandii), VTN2b (Bacillus subtilis) and VTN7 (Enterobacter cloacae) proposed as potential microbial inoculants or biofertilizers for sustainable corn production in poor Acrisols in Vietnam because of their benefit and biosafety.},
     year = {2014}
    }
    

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  • TY  - JOUR
    T1  - Isolation, Characterization and Identification of Endophytic Bacteria in Maize (Zea Mays L.) Cultivated on Acrisols of the Southeast of Vietnam
    AU  - Dang Thi Ngoc Thanh
    AU  - Cao Ngoc Diep
    Y1  - 2014/08/30
    PY  - 2014
    N1  - https://doi.org/10.11648/j.ajls.20140204.16
    DO  - 10.11648/j.ajls.20140204.16
    T2  - American Journal of Life Sciences
    JF  - American Journal of Life Sciences
    JO  - American Journal of Life Sciences
    SP  - 224
    EP  - 233
    PB  - Science Publishing Group
    SN  - 2328-5737
    UR  - https://doi.org/10.11648/j.ajls.20140204.16
    AB  - Endophytic bacterial diversity in Maize plant cultivated on Acrisols of the Eastern of South Vietnam was studied. Maize material was collected from five sites (provinces/cities) of this region. Endophytic bacteria were isolated in two kinds of medium (LGI, NFb) together with 16S rRNA gene fragments amplified from DNA using eubacterial universal primers (p515FPL and p13B). A total of 301 isolates were isolated and all of them have ability of nitrogen fixation and phosphate solubilization together with IAA biosynthesis but there were 30 isolates having the best characteristics and they were identified as maize endophytes and nifH gene owners. The sequences from selected endophytic bacteria (30 isolates) showed high degrees of similarity to those of the GenBank references strains (between 97% and 100%). Among the selected isolates were 6 isolates belong to Bacillus (20.00%) and 24 isolates belong to Proteobacteria (80.00%) including 2 alpha-proteobacteria (7%), 6 beta-proteobacteria (20%), and 16 gamma-proteobacteria (53%). Based on Pi value (nucleotide diversity), Proteobacteria group had the highest Theta values and Theta values (per sequence) from S of SNP for DNA polymorphism were calculated for each group and Proteobacteria group had the highest values in comparison of two groups. From these results showed that 3 isolates including DTN1b (Azotobacter vinelandii), VTN2b (Bacillus subtilis) and VTN7 (Enterobacter cloacae) proposed as potential microbial inoculants or biofertilizers for sustainable corn production in poor Acrisols in Vietnam because of their benefit and biosafety.
    VL  - 2
    IS  - 4
    ER  - 

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