American Journal of Life Sciences

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To Investigate the Correlation of Proton Leak and Current Produced from Animal Cells by Microbial Fuel Cells

Received: 05 April 2014    Accepted: 26 April 2014    Published: 30 June 2014
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Abstract

Proton leak has been implicated in various chronic diseases like diabetes and cancer. In this study, current from intact cells, including mice liver cells, pig blood cells and human breast cancer cell MCF-7 were measured by microbial fuel cells (MFC). Positive current change in normal liver cells were induced by either 2,4-dinitrophenol (DNP) or Piceatonnol. The effect of DNP in enhancing the proton conductivity would increase the degree of positive current change, while Piceatonnol in improving the mitochondria membrane potential would support the sustainability of the positive current change with time. Piceatonnol was found to be more effective in inducing positive current change in cancer cells than in liver cells. The higher effectiveness of Piceatonnol to cancer cells would be explained by the high proton leak condition of the cells, and so increased the current production. Little positive current change could be induced in red blood cell by either DNP or Piceatonnol. Results supported the hypothesis of the high mitochondria membrane potential to support the positive current change in cells with time, while the proton conductivity determined the degree of positive current change. The condition of proton leak of cells seemed to be the limiting factor for the positive current change in cells.

DOI 10.11648/j.ajls.20140203.17
Published in American Journal of Life Sciences (Volume 2, Issue 3, June 2014)
Page(s) 176-181
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Proton Leak, Electron Leak, Microbial Fuel Cells, 2,4-Dinitrophenol, Piceatonnol

References
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Author Information
  • Program of Food Science and Technology, Division of Science and Technology, BNU-HKBU United International College, 28 Jinfeng Road, Tangjiawan, Zhuhai, Guangdong, China

  • Program of Food Science and Technology, Division of Science and Technology, BNU-HKBU United International College, 28 Jinfeng Road, Tangjiawan, Zhuhai, Guangdong, China

  • Program of Food Science and Technology, Division of Science and Technology, BNU-HKBU United International College, 28 Jinfeng Road, Tangjiawan, Zhuhai, Guangdong, China ; Department of Chemistry, Hong Kong Baptist University, 224 Waterloo Road, Kowloon Tong, Hong Kong SAR, P.R. China

  • Department of Gastroenterology, Shanghai Jiao Tong University affiliated Sixth People’s Hospital South Campus, 6600 Nanfeng Road, Fengxian District, Shanghai, China

Cite This Article
  • APA Style

    Karen Poon, Tse Chiu Chung, Chang Xu, Ruihua Wang. (2014). To Investigate the Correlation of Proton Leak and Current Produced from Animal Cells by Microbial Fuel Cells. American Journal of Life Sciences, 2(3), 176-181. https://doi.org/10.11648/j.ajls.20140203.17

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    ACS Style

    Karen Poon; Tse Chiu Chung; Chang Xu; Ruihua Wang. To Investigate the Correlation of Proton Leak and Current Produced from Animal Cells by Microbial Fuel Cells. Am. J. Life Sci. 2014, 2(3), 176-181. doi: 10.11648/j.ajls.20140203.17

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    AMA Style

    Karen Poon, Tse Chiu Chung, Chang Xu, Ruihua Wang. To Investigate the Correlation of Proton Leak and Current Produced from Animal Cells by Microbial Fuel Cells. Am J Life Sci. 2014;2(3):176-181. doi: 10.11648/j.ajls.20140203.17

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  • @article{10.11648/j.ajls.20140203.17,
      author = {Karen Poon and Tse Chiu Chung and Chang Xu and Ruihua Wang},
      title = {To Investigate the Correlation of Proton Leak and Current Produced from Animal Cells by Microbial Fuel Cells},
      journal = {American Journal of Life Sciences},
      volume = {2},
      number = {3},
      pages = {176-181},
      doi = {10.11648/j.ajls.20140203.17},
      url = {https://doi.org/10.11648/j.ajls.20140203.17},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.ajls.20140203.17},
      abstract = {Proton leak has been implicated in various chronic diseases like diabetes and cancer. In this study, current from intact cells, including mice liver cells, pig blood cells and human breast cancer cell MCF-7 were measured by microbial fuel cells (MFC). Positive current change in normal liver cells were induced by either 2,4-dinitrophenol (DNP) or Piceatonnol. The effect of DNP in enhancing the proton conductivity would increase the degree of positive current change, while Piceatonnol in improving the mitochondria membrane potential would support the sustainability of the positive current change with time. Piceatonnol was found to be more effective in inducing positive current change in cancer cells than in liver cells. The higher effectiveness of Piceatonnol to cancer cells would be explained by the high proton leak condition of the cells, and so increased the current production. Little positive current change could be induced in red blood cell by either DNP or Piceatonnol. Results supported the hypothesis of the high mitochondria membrane potential to support the positive current change in cells with time, while the proton conductivity determined the degree of positive current change. The condition of proton leak of cells seemed to be the limiting factor for the positive current change in cells.},
     year = {2014}
    }
    

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    T1  - To Investigate the Correlation of Proton Leak and Current Produced from Animal Cells by Microbial Fuel Cells
    AU  - Karen Poon
    AU  - Tse Chiu Chung
    AU  - Chang Xu
    AU  - Ruihua Wang
    Y1  - 2014/06/30
    PY  - 2014
    N1  - https://doi.org/10.11648/j.ajls.20140203.17
    DO  - 10.11648/j.ajls.20140203.17
    T2  - American Journal of Life Sciences
    JF  - American Journal of Life Sciences
    JO  - American Journal of Life Sciences
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    EP  - 181
    PB  - Science Publishing Group
    SN  - 2328-5737
    UR  - https://doi.org/10.11648/j.ajls.20140203.17
    AB  - Proton leak has been implicated in various chronic diseases like diabetes and cancer. In this study, current from intact cells, including mice liver cells, pig blood cells and human breast cancer cell MCF-7 were measured by microbial fuel cells (MFC). Positive current change in normal liver cells were induced by either 2,4-dinitrophenol (DNP) or Piceatonnol. The effect of DNP in enhancing the proton conductivity would increase the degree of positive current change, while Piceatonnol in improving the mitochondria membrane potential would support the sustainability of the positive current change with time. Piceatonnol was found to be more effective in inducing positive current change in cancer cells than in liver cells. The higher effectiveness of Piceatonnol to cancer cells would be explained by the high proton leak condition of the cells, and so increased the current production. Little positive current change could be induced in red blood cell by either DNP or Piceatonnol. Results supported the hypothesis of the high mitochondria membrane potential to support the positive current change in cells with time, while the proton conductivity determined the degree of positive current change. The condition of proton leak of cells seemed to be the limiting factor for the positive current change in cells.
    VL  - 2
    IS  - 3
    ER  - 

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