American Journal of Life Sciences

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Determination of the Optimal Culture Medium and Salts for Bioassays with Neotropical Halophilic Cladocerans

Received: 15 February 2014    Accepted:     Published: 10 March 2014
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Abstract

The neotropical halophilic cladocerans Daphnia menucoensis and Moina eugeniae are common in shallow lakes of semi-arid central and northern Patagonia in Argentina. In the present study, we determined the tolerance of neonates of both species to salinity and different concentrations of Cl- and SO42-. However, the responses differed according to the demineralized water or groundwater employed to dissolve the salts. On the other hand, survival was lower when using analytical grade reagents instead of natural salts. As bioassays should be developed to determine the chronic physiological responses of both species, the objectives of this study were to compare three culture media and test the hypothesis that natural salts dissolved in aquifer water is the optimal combination for breeding. Acute bioassays were conducted with neonates of both species. To compare the media, NaCl solutions (5, 10, 15, 20 and 25 g.L-1) were prepared using demineralized water, aquifer water and EPA medium. For the second objective, solutions of equal concentrations were prepared with salts obtained from the natural environment, previously sterilized, and dissolved in phreatic water. Survival was higher in the aquifer water, whose chemical composition was relatively more representative of the lakes inhabited by both species. It was almost complete at 20 and 15 g.L-1 for D. menucoensis and M. eugeniae, respectively. Lower survival of D. menucoensis was found in EPA medium, although it was developed for breeding other species of Daphnia. In the second series of bioassays, survival was almost complete until 20 g.L-1 (D. menucoensis) and 25 g.L-1 (M. eugeniae). The combination natural salts/aquifer water was adequate to assess the tolerance of both cladocerans. However, the former was more important, since the results were consistent with previous studies when these salts were dissolved in demineralized water. In addition, survival was much higher than when we used salts of analytical grade.

DOI 10.11648/j.ajls.20140202.11
Published in American Journal of Life Sciences (Volume 2, Issue 2, April 2014)
Page(s) 40-45
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This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Bioassays, Daphnia Menucoensis, Moina Eugeniae, Halophilic Cladocerans Culture, Salinity Tolerance

References
[1] J. Paggi, "Cladocera (Anomopoda and Ctenopoda)", in Biodiversity of Argentinian Arthropods, S. Coscarón and J. J. Morrone, Eds. Sur Editions, La Plata, pp. 507-518, 1998. (In Spanish).
[2] G. Boxshall, and D. Defaye, "Global diversity of copepods (Crustacea: Copepoda) in freshwater", Hydrobiologia, vol. 595, pp. 195 – 207, 2008.
[3] L. Forró, N. Korovchinsky, A. Kotov and A. Petrusek, "Global diversity of cladocerans (Cladocera; Crustacea) in freshwater". Hydrobiologia, vol. 595, pp. 177–184, 2008.
[4] P. A. Battistoni, "Copepoda", in Biodiversity of Argentinian Arthropods, S. Coscarón and J. J. Morrone, Eds. Sur Editions, La Plata, pp. 519-530, 1998. (In Spanish).
[5] S. Menu-Marque, and C. Locascio de Mitrovich, "Geographical distribution of the species of genus Boeckella (Copepoda, Calanoida, Centropagidae) in the Argentina Republic", Physis, vol. 56 B, pp. 1-10, 1998. (In Spanish).
[6] S. Menu-Marque, J. Morrone and C. Locascio de Mitrovich, "Distributional patterns of the south american species of Boeckella (Copepoda: Centropagidae): a track analysis". J. Crustacean Biol., vol. 20, n° 2, pp. 262-272, 2000.
[7] S. Adamowicz, P. Hebert and M. C. Marinone, "Species diversity and endemism in the Daphnia of Argentina: a genetic investigation", Zool. J. Linn. Soc. London, vol. 140, pp. 171–205, 2004.
[8] S. A. Echaniz, A. M. Vignatti, S. José de Paggi , J. C. Paggi and A. Pilati, "Zooplankton seasonal abundance of South American saline shallow lakes", Int. Rev. Hydrobiol., vol. 91, n° 1, pp. 86-100, 2006.
[9] A. M. Vignatti, S. A. Echaniz and M. C. Martín, "The zooplankton of shallow lakes of different salinity and trophic status in the Pampean semi-arid region (La Pampa, Argentina)", Gayana, vol. 71, n° 1, pp. 38 – 48, 2007. (In Spanish).
[10] A. M.Vignatti, J. C Paggi, G. C. Cabrera and S. A. Echaniz, "Zooplankton diversity and its relationship with environmental changes after the filling of a temporary saline lake in the semi-arid region of La Pampa (Argentina)", Lat. Am. J. Aquat. Res., vol. 40, n° 4, pp. 1005-1016, 2012.
[11] M. Scheffer, "Ecology of shallow lakes". Chapman & Hall, London, 1998.
[12] E. Jeppesen, M. Søndergaard, E. Kanstrup, I. Pedersen, R. Henriksen, M. Hammershøj, E., Mortensen, J. Jensen and A. Have, "Does the impact of nutrients on the biological structure and function of brackish and freshwater lakes differ?", Hydrobiologia, vol. 275/376, pp. 15–30, 1994.
[13] E. Jeppesen, M. Søndergaard, A. Pedersen, K. Jürgens, A. Strzelczak, T. Lauridsen, and L. Johansson, "Salinity induced regime shift in shallow brackish lagoons, Ecosystems, vol. 10, pp. 47-57, 2007.
[14] R. H. Peters, "Metabolism in Daphnia", Mem. Inst. It. Idrobiol. vol. 45, pp. 193–243, 1987.
[15] M. Teschner, "Effects of salinity on the life history and fitness of Daphnia magna: variability within and between populations", Hydrobiologia, vol. 307, pp. 33-41, 1995.
[16] S. A. Echaniz, "Composition and abundance of zooplankton in lakes of different ionic composition of the province of La Pampa". PhD dissertation. National University of Río Cuarto, 2010. (In Spanish).
[17] S. A. Echaniz, A. M. Vignatti, G. C. Cabrera and S. B. José de Paggi, "Zooplankton richness, abundance and biomass of two hypertrophic shallow lakes with different salinity", Biota Neotrop., vol. 12, n° 2, pp. 37-44, 2012.
[18] M. Alonso, "Crustacea, Branchipoda", in Iberic fauna, Ramos, M. et. al. Eds, National Museum of Naturals Sciences. CSIC. Madrid, vol. 7, pp. 486, 1996. (In Spanish).
[19] L. Forró, "A new species of Moina from Australia (Crusta- cea: Cladocera)". Acta zool. hung. Vol. 31, pp. 111-118, 1985.
[20] C. Goulden, "The systematics and evolution of the Moinidae", T. Am. Phil. Soc., vol. 58, n° 6, pp. 1-101, 1968.
[21] S. A. Echaniz, G. C. Cabrera, C. Rodríguez, and A. M. Vignatti, "Do temporary lakes vary from year to year? A comparison of limnological parameters and zooplankton from two consecutive annual cycles in an Argentine temporary saline lake", Int. J. of Aquat. Sci., vol. 4, n° 1, pp. 44-61, 2013.
[22] S. E. Mitchell and W. Lampert, "Temperature adaptation in a geographically widespread zooplankter, Daphnia magna", J. of Evol. Biol., vol. 13, pp. 371-382, 2000.
[23] F. Martínez-Jerónimo and L. Martínez-Jerónimo, "Chronic effect of NaCl salinity on a freshwater strain of Daphnia magna Straus (Crustacea: Cladocera): A demographic study", Ecotox. Environ. Saf., vol. 67, pp. 411–416, 2007.
[24] M. Ghazy, M. Habashy, F. Kossa and E. Mohammady, "Effects of Salinity on Survival, Growth and Reproduction of the Water Flea, Daphnia magna", Nature and Science, vol. 7, n° 11, pp. 28-42, 2009.
[25] C. Hall and C. Burns, "Responses of crustacean zooplankton to seasonal and tidal salinity changes in the coastal Lake Waihola, New Zealand", New Zeal. J. Mar. Fresh, vol. 37, pp. 31-43, 2003.
[26] I. Heine-Fuster, C. Vega Retter, P. Sabat and R. Ramos Jiliberto, "Osmoregulatory and demographic responses to salinity of the exotic cladoceran Daphnia exilis", J. Plankton Res., vol. 32, n° 10, pp. 1405-1411, 2010.
[27] F. Martínez-Jerónimo and F. Espinosa-Chávez, "Notes on the reproduction and survival of Moina hutchinsoni Brehm, 1937 (Moinidae: Anomopoda) grown in media of varying salinity", Aquat. Ecol., vol. 39, pp. 113-118, 2005.
[28] S. S. Sarma, E. S. Nandini, J. Morales-Ventura, I. Delgado-Martínez and L. González-Valverde, "Effects of NaCl salinity on the population dynamics of freshwater zooplankton (rotifers and cladocerans)", Aquat. Ecol., vol. 40, pp. 349-360, 2006.
[29] S. A. Echaniz and A. M. Vignatti, "Seasonal variation and influence of turbidity and salinity on the zooplankton of a saline lake in central Argentina", Lat. Am. J. of Aquat. Res., vol. 39, n° 2, pp. 306-315, 2011.
[30] S. A Echaniz, A. M. Vignatti, S. B. José de Paggi and G. C. Cabrera, "The model of alternative states of shallow lakes in La Pampa: comparison of Bajo de Giuliani and El Carancho", Proceedings of 3° Pampean Water Congress, pp. 45-53, 2010. (In Spanish).
[31] A. Vignatti, R. Festa, G. Cabrera and S. Echaniz, "Comparison after a decade of limnological parameters, zooplankton richness and abundance of a shallow saline lake of the province of La Pampa", BioScriba, vol. 5, n° 1, pp. 23-35, 2012. (In Spanish).
[32] A. M. Vignatti, J. C. Paggi, S. A. Echaniz and G. C. Cabrera, "Salinity tolerance of two halophilic autochthonous cladocerans: Daphnia menucoensis y Moina eugeniae (Artropoda, Crustacea)", Biol. Acuát., vol. 27, pp. 219-231, 2012. (In Spanish).
[33] G. Cabrera, A. Vignatti and S. Echaniz, "Acute Effects of NaCl and Na2SO4 on Daphnia menucoensis Paggi, 1996 and Moina eugeniae Olivier, 1954 (Crustacea, Cladocera)", Res. in Zool., vol. 4, n° 1, in press.
[34] C. Weber (ed.), "Methods for measuring the acute toxicity of effluents and receiving waters to freshwater and marine organisms". US EPA/600/4-90/027F, Ohio, 1993.
[35] R. Sokal and F. Rohlf, "Biometrics. Principles and statistical methods in Biol. Res", Ed. Blume, Barcelona, 1995. (In Spanish).
[36] J. H. Zar, "Biostatistical analysis", 3º Ed. Prentice Hall, New Jersey, 1996.
[37] Ø. Hammer, D. Harper and P. Ryan, "PAST: Paleontological Statistics Software Package for Education and Data Analysis", Palaeont. Electr., vol. 4, n° 1, pp. 1-9, 2001.
[38] S. A. Echaniz and A. M. Vignatti, "Trophic status of shallow lakes of La Pampa (Argentina) and its relation with the land use in the basin and nutrient internal load", J. Environ. Protec., vol. 4, pp. 51-60, 2013.
Author Information
  • Facultad de Ciencias Exactas y Naturales, Universidad Nacional de La Pampa, Santa Rosa, La Pampa, República Argentina

  • Facultad de Ciencias Exactas y Naturales, Universidad Nacional de La Pampa, Santa Rosa, La Pampa, República Argentina

  • Facultad de Ciencias Exactas y Naturales, Universidad Nacional de La Pampa, Santa Rosa, La Pampa, República Argentina

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    Vignatti Alicia M., Cabrera Gabriela C., Echaniz Santiago A. (2014). Determination of the Optimal Culture Medium and Salts for Bioassays with Neotropical Halophilic Cladocerans. American Journal of Life Sciences, 2(2), 40-45. https://doi.org/10.11648/j.ajls.20140202.11

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    Vignatti Alicia M.; Cabrera Gabriela C.; Echaniz Santiago A. Determination of the Optimal Culture Medium and Salts for Bioassays with Neotropical Halophilic Cladocerans. Am. J. Life Sci. 2014, 2(2), 40-45. doi: 10.11648/j.ajls.20140202.11

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    Vignatti Alicia M., Cabrera Gabriela C., Echaniz Santiago A. Determination of the Optimal Culture Medium and Salts for Bioassays with Neotropical Halophilic Cladocerans. Am J Life Sci. 2014;2(2):40-45. doi: 10.11648/j.ajls.20140202.11

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  • @article{10.11648/j.ajls.20140202.11,
      author = {Vignatti Alicia M. and Cabrera Gabriela C. and Echaniz Santiago A.},
      title = {Determination of the Optimal Culture Medium and Salts for Bioassays with Neotropical Halophilic Cladocerans},
      journal = {American Journal of Life Sciences},
      volume = {2},
      number = {2},
      pages = {40-45},
      doi = {10.11648/j.ajls.20140202.11},
      url = {https://doi.org/10.11648/j.ajls.20140202.11},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.ajls.20140202.11},
      abstract = {The neotropical halophilic cladocerans Daphnia menucoensis and Moina eugeniae are common in shallow lakes of semi-arid central and northern Patagonia in Argentina. In the present study, we determined the tolerance of neonates of both species to salinity and different concentrations of Cl- and SO42-. However, the responses differed according to the demineralized water or groundwater employed to dissolve the salts. On the other hand, survival was lower when using analytical grade reagents instead of natural salts. As bioassays should be developed to determine the chronic physiological responses of both species, the objectives of this study were to compare three culture media and test the hypothesis that natural salts dissolved in aquifer water is the optimal combination for breeding. Acute bioassays were conducted with neonates of both species. To compare the media, NaCl solutions (5, 10, 15, 20 and 25 g.L-1) were prepared using demineralized water, aquifer water and EPA medium. For the second objective, solutions of equal concentrations were prepared with salts obtained from the natural environment, previously sterilized, and dissolved in phreatic water. Survival was higher in the aquifer water, whose chemical composition was relatively more representative of the lakes inhabited by both species. It was almost complete at 20 and 15 g.L-1 for D. menucoensis and M. eugeniae, respectively. Lower survival of D. menucoensis was found in EPA medium, although it was developed for breeding other species of Daphnia. In the second series of bioassays, survival was almost complete until 20 g.L-1 (D. menucoensis) and 25 g.L-1 (M. eugeniae). The combination natural salts/aquifer water was adequate to assess the tolerance of both cladocerans. However, the former was more important, since the results were consistent with previous studies when these salts were dissolved in demineralized water. In addition, survival was much higher than when we used salts of analytical grade.},
     year = {2014}
    }
    

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  • TY  - JOUR
    T1  - Determination of the Optimal Culture Medium and Salts for Bioassays with Neotropical Halophilic Cladocerans
    AU  - Vignatti Alicia M.
    AU  - Cabrera Gabriela C.
    AU  - Echaniz Santiago A.
    Y1  - 2014/03/10
    PY  - 2014
    N1  - https://doi.org/10.11648/j.ajls.20140202.11
    DO  - 10.11648/j.ajls.20140202.11
    T2  - American Journal of Life Sciences
    JF  - American Journal of Life Sciences
    JO  - American Journal of Life Sciences
    SP  - 40
    EP  - 45
    PB  - Science Publishing Group
    SN  - 2328-5737
    UR  - https://doi.org/10.11648/j.ajls.20140202.11
    AB  - The neotropical halophilic cladocerans Daphnia menucoensis and Moina eugeniae are common in shallow lakes of semi-arid central and northern Patagonia in Argentina. In the present study, we determined the tolerance of neonates of both species to salinity and different concentrations of Cl- and SO42-. However, the responses differed according to the demineralized water or groundwater employed to dissolve the salts. On the other hand, survival was lower when using analytical grade reagents instead of natural salts. As bioassays should be developed to determine the chronic physiological responses of both species, the objectives of this study were to compare three culture media and test the hypothesis that natural salts dissolved in aquifer water is the optimal combination for breeding. Acute bioassays were conducted with neonates of both species. To compare the media, NaCl solutions (5, 10, 15, 20 and 25 g.L-1) were prepared using demineralized water, aquifer water and EPA medium. For the second objective, solutions of equal concentrations were prepared with salts obtained from the natural environment, previously sterilized, and dissolved in phreatic water. Survival was higher in the aquifer water, whose chemical composition was relatively more representative of the lakes inhabited by both species. It was almost complete at 20 and 15 g.L-1 for D. menucoensis and M. eugeniae, respectively. Lower survival of D. menucoensis was found in EPA medium, although it was developed for breeding other species of Daphnia. In the second series of bioassays, survival was almost complete until 20 g.L-1 (D. menucoensis) and 25 g.L-1 (M. eugeniae). The combination natural salts/aquifer water was adequate to assess the tolerance of both cladocerans. However, the former was more important, since the results were consistent with previous studies when these salts were dissolved in demineralized water. In addition, survival was much higher than when we used salts of analytical grade.
    VL  - 2
    IS  - 2
    ER  - 

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