Study of Antioxidant, Antibacterial and Anti-Inflammatory Activity of Cinnamon (Cinamomum Tamala), Ginger (Zingiber Officinale) and Turmeric (Curcuma Longa)
American Journal of Life Sciences
Volume 1, Issue 6, December 2013, Pages: 273-277
Received: Nov. 30, 2013;
Published: Dec. 20, 2013
Views 3678 Downloads 430
Ranjit Thakur, Department of Biochemistry, Universal Science College, Kathmandu, Nepal； Department of Biochemistry, People’s Dental College and Hospital Nayabazar, Kathmandu, Nepal
Kamlesh Yadav, Biochemistry, Pokhara University, Kathmandu, Nepal
Khim Bahadur Khadka, Biochemistry, Pokhara University, Kathmandu, Nepal
Ethanol extract of ginger, turmeric and cinnamon was assessed for its antioxidant, antimicrobial and anti-inflammatory activity. The antioxidant activity was determined by measuring FRAP (ferric reducing-antioxidant power) assay. The antibacterial efficacy was determined using paper disc method against different gram negative bacterial and sensitivity in terms of zones of inhibition of all extract were also determined. In vitro anti-inflammatory activity was evaluated using Proteinase inhibitory assay. Aspirin was used as a standard drug for the study of anti-inflammatory activity. The result shows that the ethanolic extract of the ginger and turmeric were effective against all the bacteria tested, where as the ethanolic extract of cinnamon was failure in inhibiting the growth of all bacteria tested. The ethanolic extract of ginger possessed strong antioxidant activity in FRAP method. The ethanolic extracts of ginger shows the largest antioxidant FRAP value where as the turmeric ethanolic extract showed the minimum antioxidant FRAP value which were given as 3.86 mM/100gm and 0.38 mM/100gm respectively. The FRAP value for the ethanolic cinnamon extract was found to be 0.40 mM/100gm. The ethanolic extract of ginger and turmeric also showed in vitro anti-inflammatory activity by inhibiting the proteinase activity. Proteinase activity was significantly inhibited by ginger (78.49%), turmeric (66.48%) and cinnamon (58.72%) at 800ug/ml concentration. From the result it is concluded that the ginger, turmeric and cinnamon ethanol extract showed the antioxidant and anti-inflammatory activity where as the ginger and turmeric ethanol extract exhibited the antibacterial activity.
Khim Bahadur Khadka,
Study of Antioxidant, Antibacterial and Anti-Inflammatory Activity of Cinnamon (Cinamomum Tamala), Ginger (Zingiber Officinale) and Turmeric (Curcuma Longa), American Journal of Life Sciences.
Vol. 1, No. 6,
2013, pp. 273-277.
Fabricant DS, Fansworth NR (2001). The value of plants used in traditional medicine for drug discovery. Environ. Health Persp, 109: 69-75.
Prachayasittikul S, Buraparuangsang P, Worachartcheewan A, Isarankura-Na-Ayudhya C, Ruchirawat S, Prachayasittikul V (2008). Antimicrobial and antioxidant activity of bioreactive constituents from Hydnophytum formicarum Jack. Molecular, 13: 904-921.
Halliwell B (1995). How to characterize antioxidant: an update. Biochem. Socie. Symp, 61:85-91.
Lewis DA (1989). In: anti-inflammatory drugs from plants and marine sources. Basel: Bikhauser Verlag. Pp.135.
Taylor RSL, Edel F, Manandhar NP, Towers GHN (1996). Antimicrobial activity of Southern Nepalese medicinal plants. J. Ethnopharmacol., 45: 67-70.
Adedapo AA, Jimoh FO, Koduru S, Afolayan AJ, Masika PJ (2008). Antibacterial and Antioxidant properties of the methanol extracts of the leaves and stems of Calpurnia aurea. BMC Compl. Altern Med., 8: 53.
Lai HY, Lim YY, Tan SP (2009). Antioxidative, tyrosinase inhibiting and antibacterial activities of leaf extracts from medicinal ferns. Biosci. Biotech. Biochem, 73: 1362-1366.
Halliwell B (1996). Antioxidants in human health and disease. Annu. Rev. Nutr., 6: 33-50.
Berahou AA, Auhmani A, Fdil N, Benharref A, Jana M, Gadhi CA (2007). Antibacterial activity of Quercus ilex bark's extracts. J. Ethnopharm., 112: 426-429.
Adedapo AA, Jimoh FO, Koduru S, Masika PJ, Afolayan AJ (2009). Assessment of the Medicinal potentials of the methanol extracts of the leaves and stems of Buddleja saligna. BMC Compl. Altern Med., 9:21.
Adesegun SA, Fajana A, Orabueze CI, Coker HAB (2009). Evaluation of antioxidant properties Of Phaulopsis fascisepala C.B.Cl. (Acanthaceae). Evid. Based Compl. Altern. Med., 6: 227- 231.
Lai HY, Yau YY, Kim KH (2010). Blechnum orientale Linn - a fern with potential as antioxidant, anticancer and antibacterial agent. BMC Complem. Altern. Med., 10: 15
Alcaraz LE, Blanco SE, Puig ON, Tomas F, Ferretti FH (2000). Antibacterial activity of flavonoids against methicillin-resistant Staphylococcus aureus strains. J. Theor. Biol., 205: 231-240.
Sakat S, Juvekar AR, Gambhire MN (2010). In vitro antioxidant and anti-inflammatory activity of methanol extract of Oxalis corniculata Linn. I. J. Pharm. Pharm. Sci., 2: 146-155.
Garg VKR, Jain M, Sharma PKR, Garg G (2010). Anti inflammatory activity of Spinacia oleracea. Intern. J. Pharma Prof. Res., 1: 4.
Lin Y, Shi R, Wang X, Shen HM (2008). Luteolin, a flavonoid with potential for cancer prevention and therapy. Curr. Can. Drug Targ. 8: 634- 46.
Lopez-Lazaro M (2009). Distribution and biological activities of the flavonoid luteolin. Mini Rev. Med. Chem., 9: 31-59.
Amaral S, Mira L, Nogueira JM, da Silva AP, Florencio MH (2009). Plant extracts with anti-inflammatory properties--a new approach for characterization of their bioactive compounds and establishment of structure-antioxidant activity relationships. Bioorg. Med. Chem., 17: 1876-1883.
Singh B, Singh S (2003). Antimicrobial activity of terpenoids from Trichodesma amplexicaule Roth. Phyto. Res., 17: 814-816.
Grassman J (2005). Terpenoids as plant antioxidants. Vitam. Horm, 72: 505-535.
Neukirch H, D'Ambrosio M, Sosa S, Altinier G, Loggia RD, Guerriero A (2005). Improved anti- inflammatory activity of three new terpenoids derived, by systematic chemical modifications, from the abundant triterpenes of the flowery plant Calendula officinalis. Chem. Biodiv., 2: 657-671.
Zhang LL, Lin YM (2008). Tannins from Canarium album with potent antioxidant activity. J. Zhejiang Univ. Sci. B., 9: 407-415.
Fawole OA, Amoo SO, Ndhlala AR, Light ME, Finnie JF, Van Staden J (2010). Anti-inflammatory, ant cholinesterase, antioxidant and phytochemical properties of medicinal plants used for pain-related ailments in South Africa. J. Ethnopharmacol., 127: 235-241.
Mandal P, Babu SSP, Mandal NC (2005). Antimicrobial activity of saponins from Acacia auriculiformis. Fitoterapia, 76: 462-465.
Gulcin L, Oktay M, Kufrevioglu IO, Aslan A (2004). Determination of antioxidant activity of Lichen Cetraria islandica (L.) Ach. J. Ethnopharm., 79: 325-329.
Gepdireman A, Mshvildadze V, Suleyman H, Elias R (2005). Acute anti-inflammatory activity of four saponins isolated from ivy: alphahederin, hederasaponin-C, hederacolchiside-E and hederacolchiside- F in carrageenan-induced rat paws edema. Phytomedicine, 12: 440-444.
Das SN, Chatterjee S (1995). Long term toxicity study of ART-400. Indian Indigenous Med., 16: 117-123.