Pathogenic forms of Escherichia coli are commonly known to cause a variety of diarrheal diseases in hosts. Present study was designed to identify such pathogenic E. coli isolates from 7 different groups of street vended foods including cream, egg, non fried dry food and fried dry food, salad, cooked and non cooked items. Among 400 samples studied, almost all were found to be contaminated with an array of bacteria ranging between 3.24×102 -6.5×109 cfu/g. Results of multiplex polymerase chain reaction (PCR) assay revealed that out of 130 isolates of E. coli screened from all categories of food items, 52 (13%) were found to be enterotoxigenic E. coli, 32 (8%) were the Shiga toxin producing E. coli isolates, 14 (3.5%) were enteropathogenic and 6 (1.5%) were found to be enteroinvasive isolates. Results of plasmid profiling of the isolates was further in agreement to the presence of different pathogenic E. coli strains.
| DOI | 10.11648/j.ajls.20130106.15 |
| Published in | American Journal of Life Sciences (Volume 1, Issue 6, December 2013) |
| Page(s) | 267-272 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2024. Published by Science Publishing Group |
Street Vended Foods, Microbiological Spoilage, Escherichia coli, Antibiotic Resistance, Multiplex Polymerase Chain Reaction (PCR)
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APA Style
Nishat Sarker, Sharmin Islam, Mehedi Hasan, Farzana Kabir, Md. Aftab Uddin, et al. (2013). Use of Multiplex PCR Assay for Detection of Diarrheagenic Escherichia Coli in Street Vended Food Items. American Journal of Life Sciences, 1(6), 267-272. https://doi.org/10.11648/j.ajls.20130106.15
ACS Style
Nishat Sarker; Sharmin Islam; Mehedi Hasan; Farzana Kabir; Md. Aftab Uddin, et al. Use of Multiplex PCR Assay for Detection of Diarrheagenic Escherichia Coli in Street Vended Food Items. Am. J. Life Sci. 2013, 1(6), 267-272. doi: 10.11648/j.ajls.20130106.15
AMA Style
Nishat Sarker, Sharmin Islam, Mehedi Hasan, Farzana Kabir, Md. Aftab Uddin, et al. Use of Multiplex PCR Assay for Detection of Diarrheagenic Escherichia Coli in Street Vended Food Items. Am J Life Sci. 2013;1(6):267-272. doi: 10.11648/j.ajls.20130106.15
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Download@article{10.11648/j.ajls.20130106.15,
author = {Nishat Sarker and Sharmin Islam and Mehedi Hasan and Farzana Kabir and Md. Aftab Uddin and Rashed Noor},
title = {Use of Multiplex PCR Assay for Detection of Diarrheagenic Escherichia Coli in Street Vended Food Items},
journal = {American Journal of Life Sciences},
volume = {1},
number = {6},
pages = {267-272},
doi = {10.11648/j.ajls.20130106.15},
url = {https://doi.org/10.11648/j.ajls.20130106.15},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajls.20130106.15},
abstract = {Pathogenic forms of Escherichia coli are commonly known to cause a variety of diarrheal diseases in hosts. Present study was designed to identify such pathogenic E. coli isolates from 7 different groups of street vended foods including cream, egg, non fried dry food and fried dry food, salad, cooked and non cooked items. Among 400 samples studied, almost all were found to be contaminated with an array of bacteria ranging between 3.24×102 -6.5×109 cfu/g. Results of multiplex polymerase chain reaction (PCR) assay revealed that out of 130 isolates of E. coli screened from all categories of food items, 52 (13%) were found to be enterotoxigenic E. coli, 32 (8%) were the Shiga toxin producing E. coli isolates, 14 (3.5%) were enteropathogenic and 6 (1.5%) were found to be enteroinvasive isolates. Results of plasmid profiling of the isolates was further in agreement to the presence of different pathogenic E. coli strains.},
year = {2013}
}
TY - JOUR T1 - Use of Multiplex PCR Assay for Detection of Diarrheagenic Escherichia Coli in Street Vended Food Items AU - Nishat Sarker AU - Sharmin Islam AU - Mehedi Hasan AU - Farzana Kabir AU - Md. Aftab Uddin AU - Rashed Noor Y1 - 2013/12/20 PY - 2013 N1 - https://doi.org/10.11648/j.ajls.20130106.15 DO - 10.11648/j.ajls.20130106.15 T2 - American Journal of Life Sciences JF - American Journal of Life Sciences JO - American Journal of Life Sciences SP - 267 EP - 272 PB - Science Publishing Group SN - 2328-5737 UR - https://doi.org/10.11648/j.ajls.20130106.15 AB - Pathogenic forms of Escherichia coli are commonly known to cause a variety of diarrheal diseases in hosts. Present study was designed to identify such pathogenic E. coli isolates from 7 different groups of street vended foods including cream, egg, non fried dry food and fried dry food, salad, cooked and non cooked items. Among 400 samples studied, almost all were found to be contaminated with an array of bacteria ranging between 3.24×102 -6.5×109 cfu/g. Results of multiplex polymerase chain reaction (PCR) assay revealed that out of 130 isolates of E. coli screened from all categories of food items, 52 (13%) were found to be enterotoxigenic E. coli, 32 (8%) were the Shiga toxin producing E. coli isolates, 14 (3.5%) were enteropathogenic and 6 (1.5%) were found to be enteroinvasive isolates. Results of plasmid profiling of the isolates was further in agreement to the presence of different pathogenic E. coli strains. VL - 1 IS - 6 ER -
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