European Journal of Biophysics

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pH-Thermosensitive Oral Hydrogels Containing Phenobarbital for a Potiential Pediatric Use

Received: 22 September 2020    Accepted: 10 October 2020    Published: 16 October 2020
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Abstract

The purpose of this study was to develop a pH-thermosensitive oral chitosan-based hydrogels, able to release phenobarbital in the small intestine of the newborn. Phenobarbital is an active drug used in neonatal treatment of epilepsy. pH-thermosensitive hydrogels will improve its bioavailability and therapeutic efficiency with less side effects. This study allowed us to understand the free energy variation at the interface between chitosan chains themselves and the surrounding available molecules with its interactions behavour. Indeed, inverted tube method was used to prepare hydrogels containing 2.45 and 2.55% of chitosan, eudragitE100 and phenobarbital at 37+/-1°C via sol-gel transition. The characterization of their morphology was done by using XL SIRION 200 FEG SEM. In addition, conductivity, refractive index and density’s values were determinated. The phenobarbital release mechanism from hydrogels at different pH values, simulating the gastrointestinal tract of the newborn was also studed. UV / visible spectrophotometer SHIMADZU from the UV-2400PC series was used to determine the phenobarbital released amount as a function of time. The results showed that sol-gel transition time decreases when the chitosan concentration increases. In addition, it showed that the hydrogels structure was heterogeneous and the phenobarbital released amount were more important at pH simulating the small intestine at 2.45% of chitosan, final solution’s pH6.85 and with the presence of eudragitE100. These results were confirmed by conductivity’s values. The sols and hydrogels had a comparable refractive index and density’s values. The Korsmeyer-Peppas model was used to fit the phenobarbital release profiles. In short, the hydrogels formulated lend themselves to a phenobarbital pulsatile release usable in the newborn. The phenobarbital release profiles fitting makes possible to predict the phenobarbital amounts, which will be released at the action sites according to the need.

DOI 10.11648/j.ejb.20200802.15
Published in European Journal of Biophysics (Volume 8, Issue 2, December 2020)
Page(s) 43-51
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

pH-Thermosensitive, Oral Hydrogels, Phenobarbital, Pediatrics, Pulsatile Release

References
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Author Information
  • Physical Pharmaceutic Laboratory, Faculty of Medicine, Pharmacy and Odontology, Cheikh Anta Diop University of Dakar, Dakar, Senegal; National Center for Scientific Research, Bioactive Molecule Design and Application Laboratory, Joint Research Unit 7199, University of Strasbourg, Strasbourg, France

  • National Center for Scientific Research, Bioactive Molecule Design and Application Laboratory, Joint Research Unit 7199, University of Strasbourg, Strasbourg, France

  • Physical Pharmaceutic Laboratory, Faculty of Medicine, Pharmacy and Odontology, Cheikh Anta Diop University of Dakar, Dakar, Senegal; National Center for Scientific Research, Bioactive Molecule Design and Application Laboratory, Joint Research Unit 7199, University of Strasbourg, Strasbourg, France; Galenic and Industrial Pharmacy Laboratory, Healthy UFR of Thies, University of Thies, Thies, Senegal

  • Physical Pharmaceutic Laboratory, Faculty of Medicine, Pharmacy and Odontology, Cheikh Anta Diop University of Dakar, Dakar, Senegal; National Center for Scientific Research, Bioactive Molecule Design and Application Laboratory, Joint Research Unit 7199, University of Strasbourg, Strasbourg, France

  • Physical Pharmaceutic Laboratory, Faculty of Medicine, Pharmacy and Odontology, Cheikh Anta Diop University of Dakar, Dakar, Senegal

  • Physical Pharmaceutic Laboratory, Faculty of Medicine, Pharmacy and Odontology, Cheikh Anta Diop University of Dakar, Dakar, Senegal

  • Biophysics Laboratory, Healthy UFR of Thies, University of Thies, Thies, Senegal

  • Institute of Genetics and Molecular and Cellular Biology, National Institute of Health and Medical Research U1258, National Center for Scientific Research, Joint Research Unit 71104, University of Strasbourg, Strasbourg, France

  • Multidisciplinary Institute Hubert Curien, Multidisciplinary Institute Hubert Curien-Department of Analytical Sciences, National Center for Scientific Research, Joint Research Unit 7178, University of Strasbourg, Strasbourg, France

  • Physical Pharmaceutic Laboratory, Faculty of Medicine, Pharmacy and Odontology, Cheikh Anta Diop University of Dakar, Dakar, Senegal

Cite This Article
  • APA Style

    Alphonse Rodrigue Djiboune, Nicolas Anton, Sidy Mouhamed Dieng, Papa Mady Sy, Louis Augustin Diaga Diouf, et al. (2020). pH-Thermosensitive Oral Hydrogels Containing Phenobarbital for a Potiential Pediatric Use. European Journal of Biophysics, 8(2), 43-51. https://doi.org/10.11648/j.ejb.20200802.15

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    ACS Style

    Alphonse Rodrigue Djiboune; Nicolas Anton; Sidy Mouhamed Dieng; Papa Mady Sy; Louis Augustin Diaga Diouf, et al. pH-Thermosensitive Oral Hydrogels Containing Phenobarbital for a Potiential Pediatric Use. Eur. J. Biophys. 2020, 8(2), 43-51. doi: 10.11648/j.ejb.20200802.15

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    AMA Style

    Alphonse Rodrigue Djiboune, Nicolas Anton, Sidy Mouhamed Dieng, Papa Mady Sy, Louis Augustin Diaga Diouf, et al. pH-Thermosensitive Oral Hydrogels Containing Phenobarbital for a Potiential Pediatric Use. Eur J Biophys. 2020;8(2):43-51. doi: 10.11648/j.ejb.20200802.15

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  • @article{10.11648/j.ejb.20200802.15,
      author = {Alphonse Rodrigue Djiboune and Nicolas Anton and Sidy Mouhamed Dieng and Papa Mady Sy and Louis Augustin Diaga Diouf and Gora Mbaye and Mamadou Soumboundou and Nadia Messaddeq and Said Ennahar and Thierry François Vandamme and Mounibe Diarra},
      title = {pH-Thermosensitive Oral Hydrogels Containing Phenobarbital for a Potiential Pediatric Use},
      journal = {European Journal of Biophysics},
      volume = {8},
      number = {2},
      pages = {43-51},
      doi = {10.11648/j.ejb.20200802.15},
      url = {https://doi.org/10.11648/j.ejb.20200802.15},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.ejb.20200802.15},
      abstract = {The purpose of this study was to develop a pH-thermosensitive oral chitosan-based hydrogels, able to release phenobarbital in the small intestine of the newborn. Phenobarbital is an active drug used in neonatal treatment of epilepsy. pH-thermosensitive hydrogels will improve its bioavailability and therapeutic efficiency with less side effects. This study allowed us to understand the free energy variation at the interface between chitosan chains themselves and the surrounding available molecules with its interactions behavour. Indeed, inverted tube method was used to prepare hydrogels containing 2.45 and 2.55% of chitosan, eudragitE100 and phenobarbital at 37+/-1°C via sol-gel transition. The characterization of their morphology was done by using XL SIRION 200 FEG SEM. In addition, conductivity, refractive index and density’s values were determinated. The phenobarbital release mechanism from hydrogels at different pH values, simulating the gastrointestinal tract of the newborn was also studed. UV / visible spectrophotometer SHIMADZU from the UV-2400PC series was used to determine the phenobarbital released amount as a function of time. The results showed that sol-gel transition time decreases when the chitosan concentration increases. In addition, it showed that the hydrogels structure was heterogeneous and the phenobarbital released amount were more important at pH simulating the small intestine at 2.45% of chitosan, final solution’s pH6.85 and with the presence of eudragitE100. These results were confirmed by conductivity’s values. The sols and hydrogels had a comparable refractive index and density’s values. The Korsmeyer-Peppas model was used to fit the phenobarbital release profiles. In short, the hydrogels formulated lend themselves to a phenobarbital pulsatile release usable in the newborn. The phenobarbital release profiles fitting makes possible to predict the phenobarbital amounts, which will be released at the action sites according to the need.},
     year = {2020}
    }
    

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  • TY  - JOUR
    T1  - pH-Thermosensitive Oral Hydrogels Containing Phenobarbital for a Potiential Pediatric Use
    AU  - Alphonse Rodrigue Djiboune
    AU  - Nicolas Anton
    AU  - Sidy Mouhamed Dieng
    AU  - Papa Mady Sy
    AU  - Louis Augustin Diaga Diouf
    AU  - Gora Mbaye
    AU  - Mamadou Soumboundou
    AU  - Nadia Messaddeq
    AU  - Said Ennahar
    AU  - Thierry François Vandamme
    AU  - Mounibe Diarra
    Y1  - 2020/10/16
    PY  - 2020
    N1  - https://doi.org/10.11648/j.ejb.20200802.15
    DO  - 10.11648/j.ejb.20200802.15
    T2  - European Journal of Biophysics
    JF  - European Journal of Biophysics
    JO  - European Journal of Biophysics
    SP  - 43
    EP  - 51
    PB  - Science Publishing Group
    SN  - 2329-1737
    UR  - https://doi.org/10.11648/j.ejb.20200802.15
    AB  - The purpose of this study was to develop a pH-thermosensitive oral chitosan-based hydrogels, able to release phenobarbital in the small intestine of the newborn. Phenobarbital is an active drug used in neonatal treatment of epilepsy. pH-thermosensitive hydrogels will improve its bioavailability and therapeutic efficiency with less side effects. This study allowed us to understand the free energy variation at the interface between chitosan chains themselves and the surrounding available molecules with its interactions behavour. Indeed, inverted tube method was used to prepare hydrogels containing 2.45 and 2.55% of chitosan, eudragitE100 and phenobarbital at 37+/-1°C via sol-gel transition. The characterization of their morphology was done by using XL SIRION 200 FEG SEM. In addition, conductivity, refractive index and density’s values were determinated. The phenobarbital release mechanism from hydrogels at different pH values, simulating the gastrointestinal tract of the newborn was also studed. UV / visible spectrophotometer SHIMADZU from the UV-2400PC series was used to determine the phenobarbital released amount as a function of time. The results showed that sol-gel transition time decreases when the chitosan concentration increases. In addition, it showed that the hydrogels structure was heterogeneous and the phenobarbital released amount were more important at pH simulating the small intestine at 2.45% of chitosan, final solution’s pH6.85 and with the presence of eudragitE100. These results were confirmed by conductivity’s values. The sols and hydrogels had a comparable refractive index and density’s values. The Korsmeyer-Peppas model was used to fit the phenobarbital release profiles. In short, the hydrogels formulated lend themselves to a phenobarbital pulsatile release usable in the newborn. The phenobarbital release profiles fitting makes possible to predict the phenobarbital amounts, which will be released at the action sites according to the need.
    VL  - 8
    IS  - 2
    ER  - 

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