European Journal of Biophysics

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Comparative Studies on the Interaction of Human and Bovine Serum Albumins with Vitamin C

Received: 25 March 2018    Accepted: 08 April 2018    Published: 11 May 2018
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Abstract

The interactions of human and bovine serum albumins (HSA and BSA) with various drugs using spectroscopic techniques have received great attention now a days due to their significant effect in the biomedical field. UV absorption and fluorescence spectroscopy are the most likable due to its high sensitivity and simplicity. The interaction of HSA and BSA with vitamin C was investigated. Results showed that the absorption and fluorescence intensities increased as the vitamin C concentration increases. The calculated binding constant (k ~104 M-1) showed a week binding of vitamin C with both serum albumins. The analysis of fluorescence quenching for HSA/BSA-vitamin C interaction (kq ~ 1011 L mol-1 s-1) reveals the dynamic quenching process and clearly confirms the existence of static mechanism of fluorescence quenching.

DOI 10.11648/j.ejb.20180601.13
Published in European Journal of Biophysics (Volume 6, Issue 1, June 2018)
Page(s) 17-22
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This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Vitamin C, Human Serum Albumin, Bovine Serum Albumin, UV-Absorption, Fluorescence Spectroscopy, Binding Constant, Binding Mode

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Author Information
  • Department of Physics, Al-Quds University, Jerusalem, Palestine

  • Department of Physics, Al-Quds University, Jerusalem, Palestine

  • Department of Physics, Al-Quds University, Jerusalem, Palestine

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    Husain Alsamamra, Sawsan Abusharkh, Musa Abuteir. (2018). Comparative Studies on the Interaction of Human and Bovine Serum Albumins with Vitamin C. European Journal of Biophysics, 6(1), 17-22. https://doi.org/10.11648/j.ejb.20180601.13

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    Husain Alsamamra; Sawsan Abusharkh; Musa Abuteir. Comparative Studies on the Interaction of Human and Bovine Serum Albumins with Vitamin C. Eur. J. Biophys. 2018, 6(1), 17-22. doi: 10.11648/j.ejb.20180601.13

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    AMA Style

    Husain Alsamamra, Sawsan Abusharkh, Musa Abuteir. Comparative Studies on the Interaction of Human and Bovine Serum Albumins with Vitamin C. Eur J Biophys. 2018;6(1):17-22. doi: 10.11648/j.ejb.20180601.13

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  • @article{10.11648/j.ejb.20180601.13,
      author = {Husain Alsamamra and Sawsan Abusharkh and Musa Abuteir},
      title = {Comparative Studies on the Interaction of Human and Bovine Serum Albumins with Vitamin C},
      journal = {European Journal of Biophysics},
      volume = {6},
      number = {1},
      pages = {17-22},
      doi = {10.11648/j.ejb.20180601.13},
      url = {https://doi.org/10.11648/j.ejb.20180601.13},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.ejb.20180601.13},
      abstract = {The interactions of human and bovine serum albumins (HSA and BSA) with various drugs using spectroscopic techniques have received great attention now a days due to their significant effect in the biomedical field. UV absorption and fluorescence spectroscopy are the most likable due to its high sensitivity and simplicity. The interaction of HSA and BSA with vitamin C was investigated. Results showed that the absorption and fluorescence intensities increased as the vitamin C concentration increases. The calculated binding constant (k ~104 M-1) showed a week binding of vitamin C with both serum albumins. The analysis of fluorescence quenching for HSA/BSA-vitamin C interaction (kq ~ 1011 L mol-1 s-1) reveals the dynamic quenching process and clearly confirms the existence of static mechanism of fluorescence quenching.},
     year = {2018}
    }
    

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    T1  - Comparative Studies on the Interaction of Human and Bovine Serum Albumins with Vitamin C
    AU  - Husain Alsamamra
    AU  - Sawsan Abusharkh
    AU  - Musa Abuteir
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    DO  - 10.11648/j.ejb.20180601.13
    T2  - European Journal of Biophysics
    JF  - European Journal of Biophysics
    JO  - European Journal of Biophysics
    SP  - 17
    EP  - 22
    PB  - Science Publishing Group
    SN  - 2329-1737
    UR  - https://doi.org/10.11648/j.ejb.20180601.13
    AB  - The interactions of human and bovine serum albumins (HSA and BSA) with various drugs using spectroscopic techniques have received great attention now a days due to their significant effect in the biomedical field. UV absorption and fluorescence spectroscopy are the most likable due to its high sensitivity and simplicity. The interaction of HSA and BSA with vitamin C was investigated. Results showed that the absorption and fluorescence intensities increased as the vitamin C concentration increases. The calculated binding constant (k ~104 M-1) showed a week binding of vitamin C with both serum albumins. The analysis of fluorescence quenching for HSA/BSA-vitamin C interaction (kq ~ 1011 L mol-1 s-1) reveals the dynamic quenching process and clearly confirms the existence of static mechanism of fluorescence quenching.
    VL  - 6
    IS  - 1
    ER  - 

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