Tensile Strength of Single Collagen Fibrils Isolated from Tendons
European Journal of Biophysics
Volume 5, Issue 1, February 2017, Pages: 1-6
Received: Nov. 28, 2016; Accepted: Jan. 7, 2017; Published: Feb. 16, 2017
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Author
Noritaka Yamamoto, Biomechanics Laboratory, Department of Mechanical Engineering, Ritsumeikan University, Shiga, Japan
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Abstract
Tensile failure properties of single collagen fibrils were determined using our original tensile test method. Fibrils were directly isolated from the fascicles of mouse tail tendons. Both the ends of each fibril were wound onto the tips of two microneedles several times using micromanipulators. The fibril and tips were immersed in physiological saline solution. Then, the fibril was stretched to failure by moving the one microneedle. During tensile testing, the fibril was firmly attached to the tips of the microneedles, and broken between the tips with no slippage observed. The diameter of tested 10 fibrils was 410±60 nm (Mean±S.D.). The stress-strain curves of these fibrils were almost linear. Their tensile strength and failure strain were 100±32 MPa and 34±11%, respectively. These values were approximately 480 and 190% of those of the fascicles with the diameter of 81±12 m, respectively.
Keywords
Collagen, Fibril, Tendon, Mechanical Properties, Tensile Strength
To cite this article
Noritaka Yamamoto, Tensile Strength of Single Collagen Fibrils Isolated from Tendons, European Journal of Biophysics. Vol. 5, No. 1, 2017, pp. 1-6. doi: 10.11648/j.ejb.20170501.11
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Copyright © 2017 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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